LOCUS       sequence-472123-        2504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BEVA Golden Gate cloning vector; Narrow host range plasmid. 
            L1-pMB1-Gm-ELT3; pNDGG021, pOGG009, pNDGG007, pOGG013. GmR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Geddes BA, Williamson R, Schumacher J, Ardi A, Levin G, Cervenka E, 
            Huang R, diCenzo G
  TITLE     BEVA2.0: modular assembly of golden gate-compatible vectors with 
            expanded utility for genetic engineering.
  JOURNAL   Can J Microbiol. 2025 Jan 1;71:1-13. doi: 10.1139/cjm-2024-0246.
  PUBMED    39919271
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Can J 
            Microbiol."; date: "2025-01-1"; volume: "71:1-13. doi"; pages: " 
            10.1139/cjm-2024-0246"
COMMENT     Sequence Label: pNDGG014
FEATURES             Location/Qualifiers
     source          1..2504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    71..92
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(107..112,113..130,131..137)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    145..161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     150..172
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     169..185
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     169..185
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             181..504
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    198..254
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(255..272)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(255..271)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(264..286)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(480..499)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(609..614,615..631,632..637)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             826..1359
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     oriT            1553..1661
                     /note="incP origin of transfer"
     rep_origin      complement(1731..2319)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1811..1830)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2413..2499)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tgccggagtg agaccgcagc tggcacgaca ggtttgccga ctggaaagcg ggcagtgagc
       61 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      121 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      181 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta
      241 ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      301 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      361 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
      421 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      481 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
      541 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gacggtctca
      601 cgctgcaatt gacataagcc tgttcggttc gtaaactgta atgcaagtag cgtatgcgct
      661 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
      721 tcatggcttg ttatgactgt ttttttgtac agcctatgcc tcgggcatcc aagcagcaag
      781 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagc
      841 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taggtggctc aagtatgggc
      901 atcattcgca catgtaggct cggccctgac caagtcaaat ccatgcgggc tgctcttgat
      961 cttttcggtc gtgagttcgg cgacgtagcc acctactccc aacatcagcc ggactccgat
     1021 tacctcggga acttgctccg tagtaagaca ttcatcgcgc ttgctgcctt cgaccaagaa
     1081 gcggttgttg gcgctctcgc ggcttacgtt ctgcccaagt ttgagcagcc gcgtagtgag
     1141 atctatatct atgatctcgc agtctccgga gagcaccgga ggcagggcat tgccaccgcg
     1201 ctcatcaatc tcctcaagca tgaggccaac gcgcttggtg cttatgtgat ctacgtgcaa
     1261 gcagattacg gtgacgatcc cgcagtggct ctctatacaa agttgggcat acgggaagaa
     1321 gtgatgcact ttgatatcga cccaagtacc gccacctaac aattcgttca agccgagatc
     1381 ggcttcccgg ccgcggagtt gttcggtaaa ttgactactt ttccgctgca taaccctgct
     1441 tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat
     1501 tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga
     1561 taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc
     1621 tggcggtgct caacgggaat cctgctctgc gaggctggcc gataagctag cttgtgagca
     1681 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1741 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1801 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1861 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1921 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1981 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2041 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2101 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2161 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2221 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2281 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2341 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgattact
     2401 ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     2461 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcc
//
