LOCUS       sequence-472126-        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli expression of BirA tagged HLA-B*07:02 containing a cysteine 
            mutation for disulfide linkage to mutant beta-2 microglobulin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Sun Y, Young MC, Woodward CH, Danon JN, Truong HV, Gupta S, Winters 
            TJ, Font-Burgada J, Burslem GM, Sgourakis NG
  TITLE     Universal open MHC-I molecules for rapid peptide loading and 
            enhanced complex stability across HLA allotypes.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jun 20;120(25):e2304055120. doi: 
            10.1073/pnas.2304055120. Epub 2023 Jun 13.
  PUBMED    37310998
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-06-20"; volume: "120(25):e2304055120. 
            doi"; pages: " 10.1073/pnas.2304055120. Epub 2023 Jun 13"
COMMENT     Sequence Label: OPEN-HLA-B*07:02-BirA
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             32..54
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gtttaacsgt acattcccct ctagaaataa ttttgtttaa ctttaagaag gagatataca
       61 tatgggttct cattctatga gatatttcta cacctccgtg tcccggcccg gccgcgggga
      121 gccccgcttc atctcagtgg gctacgtgga cgacacccag ttcgtgaggt tcgacagcga
      181 cgccgcgagt ccgagagagg agccgcgggc gccgtggata gagcaggagg ggccggagta
      241 ttgggaccgg aacacacaga tctacaaggc ccaggcacag actgaccgag agagcctgcg
      301 gaacctgcgc ggctactaca accagagcga ggccgggtct cacaccctcc agagcatgta
      361 cggctgcgac gtggggccgg acgggcgcct cctccgcggg catgaccagt acgcctacga
      421 ctgcaaggat tacatcgccc tgaacgagga cctgcgctcc tggaccgccg cggacacggc
      481 ggctcagatc acccagcgca agtgggaggc ggcccgtgag gcggagcagc ggagagccta
      541 cctggagggc gagtgcgtgg agtggctccg cagatacctg gagaacggga aggacaagct
      601 ggagcgcgct gaccccccaa agacacacgt gacccaccac cccatctctg accatgaggc
      661 caccctgagg tgctgggccc tgggtttcta ccctgcggag atcacactga cctggcagcg
      721 ggatggcgag gaccaaactc aggacactga gcttgtggag accagaccag caggagatag
      781 aaccttccag aagtgggcag ctgtggtggt gccttctgga gaagagcaga gatacacatg
      841 ccatgtacag catgaggggc tgccgaagcc cctcaccctg agatgggagc cgtcttccca
      901 gtccggatcc ctgcatcata ttctggatgc acagaaaatg gtgtggaatc atcgttaaaa
      961 gcttgcgtgc actcgagcac acacacacac tgagatcgct gctacaaagc cgaaggagct
     1021 gagtgctgct gcacgctgag cattacctag cataacccct tgggggtcay a
//
