LOCUS       sequence-472127-        2767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Terminator; BEVA2.0 T2m terminator with DF extension for CIDAR MoClo
            Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR 
            amplicon into POGG006, SpR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Geddes BA, Williamson R, Schumacher J, Ardi A, Levin G, Cervenka E, 
            Huang R, diCenzo G
  TITLE     BEVA2.0: modular assembly of golden gate-compatible vectors with 
            expanded utility for genetic engineering.
  JOURNAL   Can J Microbiol. 2025 Jan 1;71:1-13. doi: 10.1139/cjm-2024-0246.
  PUBMED    39919271
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Can J 
            Microbiol."; date: "2025-01-1"; volume: "71:1-13. doi"; pages: " 
            10.1139/cjm-2024-0246"
COMMENT     Sequence Label: pNDGG037
FEATURES             Location/Qualifiers
     source          1..2767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      516..610
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(757..774)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(867..1455)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(947..966)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(2640..2744)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggcctaggc gcgccacact ctgtgcgtct catgcctgcc cggccgcttc tagagactag
      421 tgggtctcaa ggtctaacgc atgagaaagc ccccggaaga tcaccttccg ggggcttttt
      481 tattgcgccc ttgagacgct tgagaccggg acttggactc ctgttgatag atccagtaat
      541 gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat
      601 tggtgagaat ccaggcaatg agacgcacga agtgattaat taactggcct catgggcctt
      661 cacttcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aacatggtca
      721 tagctgtttc cttgcgtatt gggcgctctc cgcttcctcg ctcactgact cgctgcgctc
      781 ggtcgttcgg gtaaagcctg gggtgcctaa tgagcaaaag gccagcaaaa ggccaggaac
      841 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      901 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      961 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1021 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1081 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1141 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1201 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1261 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1321 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1381 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1441 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1501 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1561 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1621 gacagttatt atttacccac cactttggta atttcgcctt tcacgtaatg cacaaattct
     1681 tccagctgat ccgcacggct ggccagacga tcttcttcct ggcccagata cgcctgacgc
     1741 gcttccagaa tcaccggctg atactgcgcc ggcagacgtt ccatggccca atccgccgcc
     1801 acatctttcg gcgcaatttt gccggtcacc gcgctatacc aaatacggct cagggtcagc
     1861 accacgttgc gttcatcacc cgcccaatcc ggcgggctgt tccacagggt cagggtttcg
     1921 ttcagcgctt caaacaggtc ctgttccggc accggatcaa acagttcttc cgctgccgga
     1981 ccaaccagcg ccacgctatg ttcacgcgct ttggtcagca gaatggccag atcgatatca
     2041 atggtcgccg gttcaaaaat gccggccaga atatcgttac gctgccattc gccaaactgc
     2101 agctcacgtt tcgccggata acgccacgga ataatgtcat catgcaccac gatggtcact
     2161 tccaccgcac gcagaatttc gctttcaccc gggctcgcgc tggtttccag cagatcgtta
     2221 atcagcgcac gacgggtggt ttcatccaga cgcacggtca cggtcaccag cagatcaata
     2281 tcgctatgcg gtttcaggcc gccatccacc gcgctgccat acagatgcac ggccagcagg
     2341 gtcggttcca gatgacgttc aatcacgccc accacttcag acagctgggt gctcacttcc
     2401 gcaatcaccg cttcacgcat aatgttcagt ttggttttcg gacggctgcc ctgctgggtc
     2461 acatcgttgc tgctccacag catcaggcaa cggcccaccg cataacgcgc ctgctgtttg
     2521 ctcgcacgcg gcatgctctg cacgccaaaa aagcaatcat agcacgccat aaacaccgcc
     2581 accgcaccat taccgccgct acgttcggtc agggtacggc tccaattacg gctacgcata
     2641 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     2701 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     2761 gtgccac
//
