LOCUS       sequence-472128-        4104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-MFSD13A_STOP
FEATURES             Location/Qualifiers
     source          1..4104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     465..484
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     718..735
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(894..921)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1013..1099)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1148..1170
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1162..1179
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1163..1179
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1195..1294
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2855..2954)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2971..2990)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2972..2990)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2995..3011)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2995..3011)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3113..3132)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3124..3933
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3191..3210)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(4080..4104,1..564)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
       61 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      121 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      181 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      241 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      301 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      361 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      421 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      481 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      541 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      601 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      661 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      721 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
      781 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
      841 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
      901 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
      961 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     1021 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     1081 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     1141 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata
     1201 atgattttat tttgactgat agtgacctgt tcgttgcaac acattgatga gcaatgcttt
     1261 tttataatgc caactttgta caaaaaagca ggctccacca tgggactggg acagcctcag
     1321 gcatggctgc tgggactgcc aaccgcagtg gtgtacggaa gcctggccct gtttaccaca
     1381 atcctgcaca acgtgttcct gctgtactat gtggatacct tcgtgagcgt gtacaagatc
     1441 aataagatgg ccttttgggt gggcgagaca gtgttcctgc tgtggaacag cctgaatgac
     1501 ccactgttcg gctggctgtc cgatagacag tttctgagct cccagcccag gagcggagca
     1561 ggactgtcta gcagggcagt ggtgctggcc cgggtgcagg ccctgggctg gcacggacca
     1621 ctgctggccc tgtccttcct ggccttttgg gtgccatggg cacctgcagg actgcagttc
     1681 ctgctgtgcc tgtgcctgta cgacggcttt ctgaccctgg tggatctgca ccaccacgca
     1741 ctgctggcag acctggccct gtccgcccac gatcggacac acctgaactt ttactgcagc
     1801 ctgttctccg ccgcaggatc tctgagcgtg tttgcctctt atgccttctg gaataaggag
     1861 gacttctcct cttttagagc cttctgcgtg accctggccg tgagctccgg actgggcttc
     1921 ctgggagcaa cacagctgct gcggagaagg gtggaggcag caaggaagga ccctggatgc
     1981 tccggactgg tggtggattc tggactgtgc ggagaggagc tgctggtggg atctgaggag
     2041 gccgacagca tcaccctggg cagatacctg aggcagctgg cccgccaccg gaactttctg
     2101 tggttcgtga gcatggatct ggtgcaggtg tttcactgcc acttcaactc taatttcttt
     2161 cctctgttcc tggagcacct gctgagcgac cacatctccc tgtctacagg cagcatcctg
     2221 ctgggcctgt cctacgtggc cccacacctg aacaatctgt atttcctgtc tctgtgccgc
     2281 cggtggggcg tgtatgcagt ggtgagaggc ctgtttctgc tgaagctggg actgagcctg
     2341 ctgatgctgc tggcaggacc agatcacctg tccctgctgt gcctgtttat cgcctctaac
     2401 cgcgtgttca ccgagggcac ctgtaagctg ctgaccctgg tggtgacaga cctggtggac
     2461 gaggatctgg tgctgaatca caggaagcag gcagcatccg ccctgctgtt cggaatggtg
     2521 gccctggtga ccaagccagg ccagacattt gccccactgc tgggaacctg gctgctgtgc
     2581 ttttacacag gccacgatct gttccagcag tctctgatca cccctgtggg cagcgcccac
     2641 ccatggccag agccacctgc acctgcacca gcacaggccc ccaccctgcg ccagggctgc
     2701 ttctatctgc tggtgctggt gcctatcaca tgtgccctgc tgcagctgtt tacctggtcc
     2761 cagttcacac tgcacggcag aaggctgcac atggtgaagg cccagcggca gaatctgtct
     2821 caggcccaga ccctggacgt gaagatggtg taagacccag ctttcttgta caaagttggc
     2881 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     2941 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     3001 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     3061 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     3121 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     3181 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     3241 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     3301 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     3361 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     3421 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     3481 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     3541 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     3601 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     3661 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     3721 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     3781 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     3841 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     3901 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     3961 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     4021 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4081 tgagatcctt tttttctgcg cgta
//
