LOCUS       sequence-472130-        2794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Terminator; BEVA2.0 T12m terminator with DF extension for CIDAR 
            MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly 
            of PCR amplicon into POGG006, SpR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Geddes BA, Williamson R, Schumacher J, Ardi A, Levin G, Cervenka E, 
            Huang R, diCenzo G
  TITLE     BEVA2.0: modular assembly of golden gate-compatible vectors with 
            expanded utility for genetic engineering.
  JOURNAL   Can J Microbiol. 2025 Jan 1;71:1-13. doi: 10.1139/cjm-2024-0246.
  PUBMED    39919271
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Can J 
            Microbiol."; date: "2025-01-1"; volume: "71:1-13. doi"; pages: " 
            10.1139/cjm-2024-0246"
COMMENT     Sequence Label: pNDGG038
FEATURES             Location/Qualifiers
     source          1..2794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      543..637
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(784..801)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(894..1482)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(974..993)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(2667..2771)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggcctaggc gcgccacact ctgtgcgtct catgcctgcc cggccgcttc tagagactag
      421 tgggtctcaa ggtttcagcc aaaaaactta agaccgccgg tcttgtccac taccttgcag
      481 taatgcggtg gacaggatcg gcggttttct tttctcttct caacgcttga gaccgggact
      541 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
      601 gctcggttgc cgccgggcgt tttttattgg tgagaatcca ggcaatgaga cgcacgaagt
      661 gattaattaa ctggcctcat gggccttcac ttcactgccc gctttccagt cgggaaacct
      721 gtcgtgccag ctgcattaac atggtcatag ctgtttcctt gcgtattggg cgctctccgc
      781 ttcctcgctc actgactcgc tgcgctcggt cgttcgggta aagcctgggg tgcctaatga
      841 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      901 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      961 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1021 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1081 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1141 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1201 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1261 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1321 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1381 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1441 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1501 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1561 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1621 taaagtatat atgagtaaac ttggtctgac agttattatt tacccaccac tttggtaatt
     1681 tcgcctttca cgtaatgcac aaattcttcc agctgatccg cacggctggc cagacgatct
     1741 tcttcctggc ccagatacgc ctgacgcgct tccagaatca ccggctgata ctgcgccggc
     1801 agacgttcca tggcccaatc cgccgccaca tctttcggcg caattttgcc ggtcaccgcg
     1861 ctataccaaa tacggctcag ggtcagcacc acgttgcgtt catcacccgc ccaatccggc
     1921 gggctgttcc acagggtcag ggtttcgttc agcgcttcaa acaggtcctg ttccggcacc
     1981 ggatcaaaca gttcttccgc tgccggacca accagcgcca cgctatgttc acgcgctttg
     2041 gtcagcagaa tggccagatc gatatcaatg gtcgccggtt caaaaatgcc ggccagaata
     2101 tcgttacgct gccattcgcc aaactgcagc tcacgtttcg ccggataacg ccacggaata
     2161 atgtcatcat gcaccacgat ggtcacttcc accgcacgca gaatttcgct ttcacccggg
     2221 ctcgcgctgg tttccagcag atcgttaatc agcgcacgac gggtggtttc atccagacgc
     2281 acggtcacgg tcaccagcag atcaatatcg ctatgcggtt tcaggccgcc atccaccgcg
     2341 ctgccataca gatgcacggc cagcagggtc ggttccagat gacgttcaat cacgcccacc
     2401 acttcagaca gctgggtgct cacttccgca atcaccgctt cacgcataat gttcagtttg
     2461 gttttcggac ggctgccctg ctgggtcaca tcgttgctgc tccacagcat caggcaacgg
     2521 cccaccgcat aacgcgcctg ctgtttgctc gcacgcggca tgctctgcac gccaaaaaag
     2581 caatcatagc acgccataaa caccgccacc gcaccattac cgccgctacg ttcggtcagg
     2641 gtacggctcc aattacggct acgcatactc ttcctttttc aatattattg aagcatttat
     2701 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2761 ggggttccgc gcacatttcc ccgaaaagtg ccac
//
