LOCUS       sequence-472133-        4071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression in BL21(DE3) of WT T4 clamp loader consisting of gp44 and
            gp62.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4071)
  TITLE     Escherichia phage T4 Expression Constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLY1244-T4gp44/62
FEATURES             Location/Qualifiers
     source          1..4071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..85
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     172..191
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        172..190
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1897..1915)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1898..1915)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1996..2015)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2115..2137
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2175..2193)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2261..2365
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2366..2434,2435..3226)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2584..2603)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3397..3985
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3886..3905
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
       61 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
      121 ccccgcgcgt tggccgattc attaatgcag gttaacctgg cttatcgaaa ttaatacgac
      181 tcactatagg gagaccggcc tcgagtttta atagagggct tcggcccttt ataatttaca
      241 ctaaaacttg aatgaggaaa ttatgattac tgtaaatgaa aaagaacaca ttcttgaaca
      301 gaaatatcgt ccatctacta tcgatgaatg tattcttccc gcctttgata aagaaacctt
      361 taaatctatt acaagtaaag gtaagattcc acatattatt cttcattctc cttctccagg
      421 aacaggtaaa acaactgtag caaaagcatt atgtcatgat gtaaatgctg atatgatgtt
      481 tgtgaatggg tcagattgta aaattgattt cgttcgtggt cctttgacta attttgccag
      541 cgccgcttca tttgatggtc gtcaaaaagt aatcgttatt gatgaatttg accgttcagg
      601 gttagcagag tctcagcgac atcttcgttc ctttatggaa gcttatagtt caaactgtag
      661 tattattatt actgctaata atattgatgg tattattaaa ccgcttcagt cacgctgccg
      721 agttattaca ttcggtcaac caactgatga agataaaatt gaaatgatga agcagatgat
      781 tcgtcgattg actgaaatct gcaagcatga aggaattgct atagctgata tgaaagttgt
      841 agcagctttg gttaaaaaga attttcctga ttttcgtaaa actattggcg agctcgatag
      901 ttattcgtct aaaggtgttt tggatgctgg tattttatca ctggttacta acgatcgtgg
      961 tgctattgat gatgttcttg agtctctcaa aaataaagat gttaaacaac tcagagcttt
     1021 agcaccaaaa tatgctgcag attattcgtg gttcgtgggt aaacttgccg aagaaatcta
     1081 ttcacgtgta actccacaaa gtattattcg tatgtacgaa attgtcggcg aaaataatca
     1141 gtatcatggt attgcagcta atactgaatt gcatttagct tatcttttca ttcaattagc
     1201 atgcgaaatg cagtggaagt gatatgagct tatttaaaga tgatattcaa ttaaacgagc
     1261 atcaagttgc ttggtattca aaagattgga cagctgtcca atccgctgct gattctttta
     1321 aagaaaaagc agaaaatgaa ttttttgaaa taattggagc tattaataat aaaactaaat
     1381 gctctattgc tcaaaaagac tattcaaaat tcatggttga aaatgcatta tcacaatttc
     1441 cagagtgtat gccagctgta tatgctatga atttaattgg atcaggctta agtgatgaag
     1501 ctcattttaa ttatctaatg gctgcagttc ctcgtggtaa aagatatggt aaatgggcaa
     1561 aactggttga agattccacc gaagtattga ttattaagtt acttgctaag cggtatcaag
     1621 ttaatacaaa tgatgcaatt aactataaat caattcttac taaaaatgga aaacttcctt
     1681 tagtattaaa agaactaaaa ggtttagtca cggatgattt tttgaaagaa gtgactaaga
     1741 acgtaaaaga acagaaacaa ctcaaaaaac tagcattgga atggtaaaat gattgaaatt
     1801 actcttaaaa aacctgaaga ttttcggaaa gtaaaagaaa gcttgactcg tatggggggt
     1861 accgagctcg aattcatcga tgatatcaga tctggttcta tagtgtcacc taaatcgtat
     1921 gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtccat
     1981 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     2041 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     2101 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     2161 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     2221 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2281 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2341 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2401 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2461 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2521 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2581 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     2641 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2701 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2761 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2821 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     2881 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     2941 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3001 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3061 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3121 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3181 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3241 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3301 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3361 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3421 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3481 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3541 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3601 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3661 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3721 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3781 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3841 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3901 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3961 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4021 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt c
//
