LOCUS       sequence-47214-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3110-01
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..726
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="AIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKEG
                     GPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIARND
                     ITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLLEG
                     NAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDNA
                     RR"
     primer_bind     complement(802..826)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 ggatccccgg gcttaagatc ggtgacggtg ctggtttaat taacacngan tgcgattaag
       61 ccagacatga agatcaaact ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc
      121 gacggagatg gtacaggcaa gccttttgag ggaaaacaga gtatggatct tgaagtcaaa
      181 gagggcggac ctctgccttt tgcctttgat atcctgacca ctgcattcca ttacggcaac
      241 agggtattcg ccaaatatcc agacaacata caagactatt ttaagcagtc gtttcctaag
      301 gggtattcgt gggaacgaag cttgactttc gaagacgggg gcatttgcat tgccagaaac
      361 gacataacaa tggaagggga cactttctat aataaagttc gattttatgg taccaacttt
      421 cccgccaatg gtccagttat gcagaagaag acgctgaaat gggagccctc cactgagaaa
      481 atgtatgtgc gtgatggagt gctgacgggt gatattcata tggctttgtt gcttgaagga
      541 aatgcccatt accgatgtga cttcagaact acttacaaag ctaaggagaa gggtgtcaag
      601 ttaccaggct accactttgt ggaccactgc attgagattt taagccatga caaagattac
      661 aacaaggtta agctgtatga gcatgctgtt gctcattctg gattgcctga caatgccaga
      721 cgatagggcg cgccacttct aaataagcga atttcttatg atttatgatt tttattatta
      781 aataagttat aaaaaaaata agtgtataca aattttaaag tgactc
//
