LOCUS       sequence-472143-       25104 bp    DNA     circular UNA 15-DEC-2025
DEFINITION  Optimized thiosulfate sensor with acoustic reporter genes (bARGSer).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 25104)
  AUTHORS   Buss MT, Zhu L, Kwon JH, Tabor JJ, Shapiro MG
  TITLE     Probiotic acoustic biosensors for noninvasive imaging of gut 
            inflammation.
  JOURNAL   Nat Commun. 2025 Aug 25;16(1):7931. doi: 10.1038/s41467-025-62569-1.
  PUBMED    40855083
REFERENCE   2  (bases 1 to 25104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-08-25"; volume: "16(1):7931. doi"; pages: " 
            10.1038/s41467-025-62569-1"
COMMENT     Sequence Label: MB 38t3 thsS(t3)R-bARGSer
FEATURES             Location/Qualifiers
     source          1..25104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1241..1275
                     /label=BBa_J23104
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.72"
     terminator      3111..3182
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3198..3225
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     3292..3310
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3331..3365
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     terminator      4029..4100
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4116..4143
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             13561..13572
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(20199..20210)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(21317..21334)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      21487..21573
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      21665..21692
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        21711..21802
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      22260..22715
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(22347..22366)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     22557..22578
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(23271..23930)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWCEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     23845..23864
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(23931..24033)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(24425..24442)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(24559..25104)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(24639..24658)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcatc ttattaatca gataaaatag ggttatttcg atgaaaggag agagtactaa
       61 tgccaattgc aataggaaat aaacggttac ccgttacgtt ggatgaaaag agacaaaaag
      121 aattgcagca actaaagcag aagtacggca aaagtgaatc caggattatg tgtattgcgt
      181 tagatttatt gattgcccaa gaaaaagcag gatttgaggt accagcactc aaaaagtgac
      241 gtcacttttt atcctaaaaa ctaaaagtga tagcactttt aattataaga agttagaata
      301 ttaatcattt gcttaattgt acaatataat gtacaattgt tttatagaaa taaataaggg
      361 gtgaaaggaa tggaagcagt agcttattca aatttccgcc aaaatttacg tagttatatg
      421 aaacaagtta atgaggatgc tgaaacactt attgtaacaa gtaaagatgt agaagataca
      481 gttgttgtat tatcaaaaag agattatgat tctatgcaag aaacgttgag aacactttct
      541 aataattacg tcatggaaaa aattcgtcga ggagatgaac aattctccaa aggtgcattt
      601 aaaacacatg acttaatcga ggttgaatct gatgattaag gcttggtctg atgatgcttg
      661 ggatgattat ctttattggc atgagcaagg aaacaaaagc aatataaaaa agattaacaa
      721 gttaataaaa gatatcgatc gttccccctt tgctggatta ggaaaacctg agccattaaa
      781 gcatgattta tctggaaaat ggtccagaag aattacagat gaacatagac tgatatatag
      841 agttgaaaat gaaacgatat ttatttattc tgcaaaagat cactattaac caatcggaag
      901 taaggaaagg gtcagaaact taaaagtttt tgatccttat tttatttacc ctagtcattt
      961 aaaaagctaa tatagcttag tgttgattgt tattaatgaa tgtgtttgtt acgcgtatta
     1021 cggatataag gttagtaaaa tcatttctaa agttgaggaa aagtaaatat aaatggctta
     1081 aatttcaaca atttgaagtt gaatagatat gttataatac tattgtagtg tgggatgtta
     1141 gttactaaag gatgacgctt atatatatga ctgaatagaa taagcaatag gtttaataat
     1201 ctattttaaa ttttttgtac tagttttagt taattagcgg ttgacagcta gctcagtcct
     1261 aggtattgtg ctagcctagt atcgatctcc ataactatcc tatagatcat gtcccgcctg
     1321 ctgctgtgta tctgtgttct gctgttctct tctgtggcgt ggtctaaacc gcagcagttt
     1381 tatgtgggcg tactggctaa ctggggtcat cagcaagccg ttgaacgttg gaccccgatg
     1441 atggagtatc tgaacgaaca cgtgccggac gcggaatttc acgtctaccc gggcaacttc
     1501 aaagcactga acctggcaat ggaactgggc cagattcagt tcattatcac taacccgggc
     1561 caatatctgt acctgagcaa tcagtacccg ctgtcttggc tggcgaccat gcgttctaag
     1621 cgtcacgatg gtaccacttc tgcgatcggt tccgccatta ttgtccgcgc ggacagcgac
     1681 taccgcaccc tgtacgacct gaaaggtaaa gtggtggctg cgtccgaccc gcatgctctg
     1741 ggtggctacc aagcgaccgt cggtctgatg cattccctgg gcatggatcc ggacaccttc
     1801 ttcggtgaaa ccaagtttct gggctttcca ctggatccgc tgctgtacca agttcgtgat
     1861 ggcaacgttg acgcggccat taccccactg tgcactctgg aggacatggt tgcacgcggc
     1921 gtactgaaat cttccgattt tcgtgtgctg aaccctagcc gcccggatgg tgtagaatgc
     1981 cagtgctcta ccaccctgta cccgaactgg tctttcgctg cgactgagtc tgtatccacc
     2041 gaactgtcta aagaaatcac gcaggcactg ctggaactgc catccgacag cccggcagct
     2101 atcaaagcgc aactgaccgg ctggaccagc ccgatctccc aactggcggt aatcaaactg
     2161 ttccaagagc tgcacgtaaa aaccccggac tctagccgtt gggaagccgt taagaagtgg
     2221 ctggaagaaa accgtcactg gggtatcctg tctgttctgg tgttcatcat tgcaacgctg
     2281 tatcacctgt ggattgaata ccgcttccac caaaaaagct cctctctgat cgaatctgaa
     2341 cgtcagctga aacagcatgc tgttgccctg gaacgtctgc aatctgctag catcgttggt
     2401 gaaattggtg cgggtctggc ccacgagatt aatcagccga tcgctgcaat tacctcttat
     2461 tctgaaggtg gcatcatgcg cctgcaaggt aaagaacagg cggatacgga tagctgcatc
     2521 gaactgctgg aaaaaatcca caaacagagc actcgcgcag gcgaagtggt gcaccgcatc
     2581 cgtggtctgc tgaaacgtcg tgaagcggtg atggtagatg ttaacatcct gaccctggtg
     2641 gaagaatcca tcagcctgct gcgtctggag ctggcacgtc gcgaaatcca gatcaacact
     2701 cagatcaaag gtgaaccgtt cttcattact gccgaccgcg ttggcctgct gcaagttctg
     2761 attaacctga tcaaaaactc cctggacgcg atcgctgaat ctgataatgc ccgttctggt
     2821 aaaatcaaca tcgaactgga ctttaaagag taccaggtaa acgtctccat catcgataac
     2881 ggtccgggcc tggcgatgga ttctgacact ctgatggcta cgttttacac taccaaaatg
     2941 gatggcctgg gcctgggtct ggcagtctgc cgcgaagtta tcagcaacca cgacggccac
     3001 atcctgctgt ccaaccgtga cgacggcgtt ctgggctgtg tggcaaccct gaatctgaaa
     3061 aaacgcggtt ctgaagtgcc gatcgaagtc taataatcta gaccaggcat caaataaaac
     3121 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     3181 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat aggtacccgg
     3241 ggatcctcta gcgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     3301 atcacgaggc cctttcgtct tcacctcgag tttacggcta gctcagtcct aggtactatg
     3361 ctagcagaaa tataaagaac gatctattta tccgcgtaca tgcagcagca aatcaacggc
     3421 ccggtctacc tggtggatga tgatgaagcc attatcgact ccatcgattt tttgatggag
     3481 ggctacggtt acaaactgaa ctcgtttaac tgcggcgatc gctttttggc agaagtcgat
     3541 ctcacccagg caggatgtgt aattctggat gcgcgtatgc caggcttaac tggtcctcag
     3601 gtgcaacagc tgctgagcga cgcgaaaagc ccgcttgcgg tcatcttcct gaccggccat
     3661 ggcgatgttc cgatggcggt tgatgcgttc aaaaatggcg cgttcgattt ctttcaaaaa
     3721 cctgtgccgg gtagcttgct cagtcagtca attgccaaag gcttgactta ttcaatcgat
     3781 caacatctga aacgtactaa ccaagcgtta atcgacacgc tctcggaacg cgaagctcaa
     3841 atttttcaac tggtgattgc aggcaacacc aacaaacaga tggctaacga gctttgcgtg
     3901 gctattcgta ccattgaggt tcaccgtagc aaactgatga ccaaactggg tgttaacaac
     3961 ctggctgaac tggttaaact ggcgccgctg ctggcacata aatccgaata ataatctaga
     4021 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     4081 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     4141 gcgtttatag taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata
     4201 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctggttagc
     4261 tagtcattca agcattatta tgctgttttt tgaagtgaat gtgcggccat ctagccgcac
     4321 attttgcatc taaaacatgc agtcatcagc aaaataataa acttttcccc aatatgtggt
     4381 ttaccacaat ttacaggaat tcactcctgt ggtggtgcaa atttgaactg tgaattgctt
     4441 cacaaacgcc gctatcgcaa tgtcagtatg tggtttacca caatatctaa tatcactctg
     4501 ctcaataaca atgatgaaaa ccttaggaag aagttaattg tgttaaacag ttaactaggg
     4561 gctttatcta acgctctcct aaggacaact gtcattggga gatttaactt cattgtggtg
     4621 gatttattaa cattaataga gcgctgatgc ctataggttg gcgcatgagt tgcattctac
     4681 atgggcgtgc agtaacaagt ctgtacgtag aacctaatta catatgtaat ttattaacat
     4741 tttctaggag acgccatcat ggctaaagta caaaaatcaa cagactcttc cagcttggct
     4801 gaagtagtag accgtattct ggacaaaggc attgtgattg acgcttgggt caaagtctca
     4861 ttggttggta ttgaactgtt atccatcgaa gcacgtgttg ttattgcttc tgttgaaact
     4921 tatttgaaat acgcagaagc gattggcctt acagccagtg cagcgactcc agcgtaatta
     4981 agtaagcaga ataaaagtgc ccaagtggct gagtcattca gctgcttgag tacttttcaa
     5041 cgcttcaaga gtgctctatc acataaggag aatcctatgg gatgcttaac agacggtatg
     5101 gcgcagctgc gtaagaatat tgatgacagt catgaatctc gcatagcaca gcaaaatgcg
     5161 cgggtttcca gtgtgagtgc gcagattgcc ggtttttcta caactcgggc tagaaacgcg
     5221 gctcaagacg cacgcgcacg cgcgacattc gttgccgata atgtcagagg cgttaatcgg
     5281 atgttgagtg acttctgtca tacccgcgag gtaatgagtc gtcaacagag tgaagaacgt
     5341 gccaccttcg tgacggatat gtcgaagaaa acgctagctt tattagacgg ttttaatgct
     5401 gagcgtaaaa gtatggctga acgctgtgct aaagaacgag ctgatttcat tgccaacgtg
     5461 gcaaacgatg tcgctgcttt tcttagtgct tctgagaagg acagaatggc tgcccatgct
     5521 gtatttttcg gcatgaccct ggctaaaaaa aaaacgagcc tcgcagtcta gccactcgtc
     5581 ctcttgataa tactctggcg gtaagtagtg aagatgaaaa aaggtaagta gcggtattag
     5641 cctctgtcgt ccatggaggg tgaggtggat gaacagtctg ctcacaaaca cggagtgaca
     5701 ttattgggca tacagtttcg attaatcgct aagttaaatc gaaatataag aatccgctaa
     5761 aatcgaccga ttctagcggc taagacgatc actgatttgg ccatttcgta gaactggtgt
     5821 tggctagggc gtacacggtt tggcacagga gtatttccat gattaagcaa aataccgttt
     5881 cgcagtatac cgtagacgat gatcttgtcg ttccggaagc gagtgaacat tttgtagcga
     5941 cttcttatgt gaacgacatt atagaacgcg cattggttta tttacgcgct ggctatcccg
     6001 tgcattttgc cggcccatct gggataggga aaacaaccct ggcatttcat ctcgcagcac
     6061 tatggggtcg tcccgttacc atgttgcaag gtaacgaaga atttgtgagc tccgatttga
     6121 cggggaaaga catcggctat cgtaaaagca gcctggtgga taactacatt cattcagtgc
     6181 ttaaaactga agaacagatg aaccgcatgt gggtggataa ccgtttgacc accgcgtgtc
     6241 gtaacggcga catgctgatc tatgatgagt tcaatcgttc caaagcagaa accaataatg
     6301 tgctgttatc cgtattatcc gaagggattt taaaccttcc tggtttacgt ggtgtagggg
     6361 aagggtatct cgatgtacac ccagaatttc gcgctatttt tacctccaat ccagaagagt
     6421 atgccggtac gcataagacc caggacgctc tgatggatcg tatgattact atcaacattg
     6481 gtcttgtcga tcgggatact gaactacaga tactgcatgc gcgctctgaa ttggaattaa
     6541 aagaagcggc atatatcgtt gatataatca gagagttgcg tggtaacgaa cacgaaacca
     6601 aacatgggtt acgtgcagga attgccatcg cccacatcct acaccagcaa ggaataaaac
     6661 cacgttatgg tgacaaatta ttccatgcta tctgctacga cgtcttgagt atggacgctg
     6721 ccaaaataca acacgctgga cgttctatat accgtgaaat ggtcgatggc gttattcgta
     6781 aaatctgtcc accaataggt tcagacacgg ttaaagcatc cacacaaaaa ataaaagcag
     6841 tggagtaatt atcatggcta taagtactcg tcctctcaga acactgtcag acataaaaac
     6901 tcattcaggt cgagtgagtg gtgagcatca aacctatcgt gattattttc agataggtgc
     6961 gctggaatta gagcgttggc ggcgaacacg agaacgtgag gctgcctcat cgcgtattgc
     7021 cagtattgat gagcgtatcg cggatattga taaagaaaaa gcagcgctgc ttgcagacgc
     7081 gacagcggcg agtgctgtgg ctgaaaataa tgataaatca gaagcggccg agaagaagaa
     7141 aaagtcttct ggtttgcgaa tcaagtattg aggttgtcat tatgaacaaa attcgcaccc
     7201 tcgctcatat ccgtacaccg cgtatggtga agagctttcg cacatgcgtc aacatgacgg
     7261 ggaagtcaca aattctcgct gattttggac gtatttattt agaagaatat cgacgattga
     7321 accgtgtgca atgcgccaaa agtaaacgta tttcattgag gagtgcatga tgagtattga
     7381 taaatccaga aatcatcgtg ccaaggtgct ttatgcgctc tgtgtttccg atgactcaac
     7441 gcctaactat aaaatacgcg gattggaagc agcaccggtt tattctattg atcaagatgg
     7501 cttaagagct gtggtcagcg atacgctcag tacccggtta cgtccagaaa gacgtaatat
     7561 cacggcgcat caggctgtat tacataagtt gactgaagaa ggtaccgtat tgccgatgcg
     7621 ctttggggtt attgcccgta atgctgaagc agtaaagaat ttgctggtcg ctaatcagga
     7681 tacgatcaga gaacattttg aacgcttgga tggctgtgtg gaaatggggc tacgtgtttc
     7741 ctgggatgtt actaatattt atgaatattt tgttgctaca tatccagtct taagtgaaac
     7801 gcgcgatgaa atctggaatg gaaattccaa tgccaataat caccgtgaag agaagattcg
     7861 gctgggtaat ttgtacgagt cattgcgtag tggagacaga aaagaatcga cggaaaaagt
     7921 aaaagaagtt ctgttggatt attgcgaaga gattattgaa aacccggtta aaaaagaaaa
     7981 agacgtaatg aatctagcct gtttggtagc aagagaacgg atggatgaat ttgccaaagg
     8041 cgtttttgag gcatcaaagt tattcgataa cgtttatctg ttcgattata ccggcccatg
     8101 ggcaccgcat aatttcgtta ctctggattt acatgctcct acagcgaaga agaaaacgtt
     8161 gacccgcgct ggtactctct ctgactgagg ctaagccatg ctgctgatcg atgacatcct
     8221 gttttctcct gtcaaaggcg tcatgtggat ttttcggcaa attcacgaac tggcggaaga
     8281 tgaattggct ggtgaggcgg atcgtattcg tgaaagtttg actgatcttt atatgttact
     8341 ggagactggc cagattaccg aagatgaatt tgaacaacag gaagctgtac tgctggatag
     8401 attagacgca ctggatgaag aagatgacat gctaggcgac gagcctgggg atgacgaaga
     8461 cgacgaatac gaagaggacg acgacgaaga ggacgacgac gaagaggatg acgacgacga
     8521 ggatgacgac gacgaggatg acgacgacga agaggatgac gacgacgacg aggatgacga
     8581 cgacgaggat gagccagagg gtacgacgaa atgaaacctg ctatttatcc caagttctta
     8641 ctggagtcgc cgctgaaact ggttttcttc ggcggtaagg gaggagtagg taaaagtacc
     8701 tgtgcgacgt cgacggcgtt aaggctggcg caggaacagc cgcagcacca ttttttgctg
     8761 gtttctaccg atccagcaca ttccctgcaa aatattctct ccgatctggt attgccaaaa
     8821 aatctggacg tacgtgaatt aaatgccgca gcctccttgc atgaatttaa atcgcagcat
     8881 gaaggtgttc tgaaagagat agcgtatcgc ggcactgttc tcgatcaaaa cgacgtacaa
     8941 ggattgatgg ataccgcgct tcccggcatg gatgaattgg ccgcttatct tgaaatagct
     9001 gaatggattc aaaaagatac ttactatcgt attattattg atactgctcc tacaggtcat
     9061 acattgcggc ttttggaaat gccggatttg atttatcggt ggttgaccgc tctggatacc
     9121 ctgcttgcca aacagcgtta tatccggaaa cgttttgccg gtgataatcg attggatcat
     9181 ctggatcatt ttctgctgga catgaatgat tcacttaaag ctatgcacga gctggtgacg
     9241 gattccacgc gttgttgttt tgtgttagtg atgctggcgg aagcgatgag tgtggaagaa
     9301 agcattgatc ttgctggcgc gttgaatcag caacgggtgt ttctgtccga tttagtggtc
     9361 aatcgactat ttcctgaaaa tgactgtcca acctgttgtg ttgagcgtaa cagacaaatg
     9421 cttgcgctcc agaacggata ccaacgtttg cctgggcacg tattctggac gttgcctttg
     9481 cttgccatcg aaccgcgtgg tgcgttgctt cacgaatttt ggtccggggt ccgtttactt
     9541 gatgaaaacg aagtcatggc tacgacgtgt catcatcaac tgcctttacg tgtagagagt
     9601 tccatttcgc tgcctgccag cacattccga ctgctgattt ttgcaggtaa aggtggggta
     9661 ggtaaaacga cgctggcctg tgctacggca ttgcgtctga acagtgaata tccagaattg
     9721 cgtattctgt tgttctcagc cgatcctgct cactcgctga gtgattgtct gggtgtaacc
     9781 ctacagcagc agcctatatc ggtgttggtt aacattgatg cccaggaaat taatgcacaa
     9841 gctgattttg acaaaatccg gcagggatat cgtgcggaat tagaagcttt cttgctggat
     9901 acgttaccca atctggatat tacttttgat cgtgaagtgt tggagcattt gctcgatctc
     9961 gcgccccccg gattagatga aatcatggca ttgactgcga tcatggatca tctagacagc
    10021 ggtcgttatg acatggtcat cgtggatggc gcacctagcg gacatttgct acgtttatta
    10081 gagttgcctg aattgattcg ggattggcta aaacagtttt tctcgttatt gctgaaatat
    10141 cgcaaagtca tgcgtttccc gcatttatcg gaacgtttgg tacagctttc acgcgaatta
    10201 aaaaatctac gcgctctact gcaggatacc aaacaaacgg ggttgtatgc agttactgtt
    10261 ccgacgcatt tggcacttga gaagacctat gaaatgacat gcgcattgca gcgattaggc
    10321 ttaacggcaa acgcgttgtt tatcaaccag attacacctc ccagcgattg cacactgtgc
    10381 caggcaatca cgagtcgaga gtcgttggag ctgaagtgcg cagatgagat gttccacagc
    10441 caaccacacg cgcagatttt ccggcaaaca gaacctacgg gattgagcaa gctaaagaca
    10501 ttgggttcgg cattgttttt ataaactgtg agcgtacaca ttatgccagt gaacaaacag
    10561 tatcaggatg aacagcaaca ggtctctctg tgtgaggcct tggaccgcgt tctgaacaaa
    10621 ggtgtggtga ttgtcgctga tatcaccatt tcagtcgcta atattgattt gatttattta
    10681 agcctacagg ctttggtttc ttctgtagaa gcgaaaaacc gtttacccgg tagggagtaa
    10741 cactatgaca accaatcagt tatcgcacca tagtcctgtg ttcggcccga catcgccagc
    10801 aatacaacgg ccaattactg aagctaatcg acataaaata gacatcgatg gagaacgcgt
    10861 ccgtgatggt ttggcgcaac tcgtcctgac cctggtcaaa ctgctgcacg aactactgga
    10921 gcgtcaagcc ataaggcgga tggattcggg ttcgcttagt gatgaagaag tcgagcgttt
    10981 gggtttggcg ctaatgcgcc aagccgagga gttgacacat ttgtgcgacg tttttggctt
    11041 caaggatgat gatttgaact tggatttagg cccgctaggc cgattgctgt aaggaaattg
    11101 tatgatgaac aacaacatac gattattttt tagttcccat ataaatgtta tcagtctgtt
    11161 ttcggcagag ggtgtgtacc atggttaata ctaccaatga cataaatgcg gctacgcgtg
    11221 gtcttctact gcgtatgggt aatgcatggt ttgaacaaga tgaactgcga caagcagtgg
    11281 atatctatct gaaaataatc gagcaatatc ctgacagcaa ggagagtaaa accgcgcaaa
    11341 cggcgctgtt gaccatttca caacgttatg aacgagatgg tttgttccgg ctgtcacttg
    11401 atatccttga acgcgttggg gaaataacac cgacatccat ataagtttca ggatattaac
    11461 gtggcctatt tatcaggttt gaatcctata ttaagtcgca agcgatatcg ccagcagtgt
    11521 tttctacgca gtgagatcca tgttcaggat accctggatc caagaattaa gagcatcagt
    11581 acggtataga tcatctttca agttgcaggt gcgttgtctg cccacgctaa ccccagtcac
    11641 ttactggagt aagccttggg gatttactcg attgtcgcct tcctgtgtgt tgaaatttat
    11701 tgggtatata tgcttttgat tttagagaga aggggatgag gatttgaggt ctttaggttg
    11761 tttaccctat aaggatttgc accatgagcg gcaacaaaaa actaacccat tcaaccgatt
    11821 cgaccaccgt agcggatttg ttagagcgat tactggacaa aggggttgtc atcagcggtg
    11881 acattcgcat cagactggtt gaagtcgaat tgctgacact ggaaattcgc ctgcttatct
    11941 gttccgtaga taaagcggta gaaatggggc tggattggtg gagtggcaat ccggcgtttg
    12001 attccagagc cagagtgtca tcatcagcgc ctgctccaga gttggaagaa cgtctgcagc
    12061 ggctagaggc tcgtttggaa gcggcaccgt ccgtcataga agaaactcat ttataaggat
    12121 tatctttttt atgcgagcgc tgattcattt tcccattatt catagcccta aagatttagg
    12181 gacattgagc gaagcggcaa gtcatttgcg aaccgaaact caaacgcgtg cgtatcttgc
    12241 tgccgtcgag gggttctgga caatgattac cactacgatt gagggtttag atctggacta
    12301 tacacatctg aaactttatc aggatggtct gccggtttgt ggaaaagaaa atgaaatagt
    12361 gaccgatgtt gctaacgctg gtagccagaa ttacaagctc ttgcttacgt tgcaacataa
    12421 aggcgcaatt ctgatgggaa cagagtcccc tgaactattg ttgcaagagc gcgatctgat
    12481 gacgcagttg ttacaatcta cggagcaaac cgaagcgtcc ttggaaacag caaaaacatt
    12541 attgaaccgg cgggatgatt atattgccca gcgcattgat gaaacgctgc aagatggcga
    12601 gatggcgata cttttcctgg ggcttatgca caatattgaa gcgaagctgc ccgcagatat
    12661 tgtttttatt caaccgttag gtaaaccccc aggaggggaa agtatttagt gactacattc
    12721 cttgttctgc attatcgttg gcattagaag tggatttatt caccgagaat attagaggta
    12781 gtataaatat aatttatatg tcattttaat agactacata ataatgggac tttgttgctt
    12841 tttttatatt tttatgcgtt tttttgactg atgaaaaacg taataataac caactatgtt
    12901 tgtttgtaaa agtatcggca tagcagcaat atagtgcgta tacggtgata aataaatatt
    12961 atggaacccg tggtcggaat ttccatcacg ggatgatttt gttaatacta cccttcctgt
    13021 ttgtctgttt cagccttcat ttttgtgtaa agaaatcgaa atcaatattc attattttgt
    13081 tagataatat atggtgcttc tagtggaatt gatgccagat gtgacatagg caaacgtttt
    13141 ttggcggtgg tttattagct atttatgtga tgtatgtctc ttagttatta ccagttttat
    13201 cgatagccct atgatctttt gatactaaaa atgtgaccta atcggctaaa aagggagtgt
    13261 cgcccttgat atggctgata gccatcagta gacagatgat gggatgatgt ttatcatgtc
    13321 cagaataaag tctctcgctt atttgaccgg gtagttaaaa tattcgttaa taaatagaaa
    13381 tacttgccta aattattgtc acaaaatcga aaagaaaatt gtgttatctt aaatttgtac
    13441 tataagtatt aaggatgcta ttcattaaaa ttaggcaccg tctaggcgat gattaggcgg
    13501 ttgtgttaaa aaaataacta gagtgttaat aattcttttt ttgcgtcaaa tatatacgtg
    13561 atagaaggga gatttatcct cttcttcccc gtatctttta actattatta aaaatgaaat
    13621 aaccaattgg atgtagtcga tgaaaagtat tgatcctggc tatgttttaa taattgatga
    13681 cgacaaagat atttgtgacc tgctatcaca cttgctggaa aaggctggtt gtgaaactca
    13741 gcaaggttac gatggcgaca tggcaataaa gttgttgtct cagcgtgaac cggatgtact
    13801 gttacttgat agtattctcc ctaaacacag tggcatggag gtgttagcca atgcccatgc
    13861 tctgtatccg caactccccg ttatcattat tactggcaat gtcggcattc ttagcgcggt
    13921 tggtgctatc aaagctggtg catgggatta cattcccaag ccgtttgata acaaacgagt
    13981 attagatctg gtgaatcgtg caatgcaaat gcgttggggt aactacgatt caaactgcaa
    14041 agccagcaag gatactaaaa cgcgtatcgc tgccatgatg gggaatagcc gcgagattca
    14101 gtcggtgatc agagatgtcg ttaacgtggc tcatacggat ttttctgtcg ttattcaggg
    14161 tgaaacggga actggcaaag aattggttgc gaaaaacatc catttagcca gtcagcatgc
    14221 cacaggggtt tttgttccca ttgattgtgg ctccataccc gactcgttga tcgaaaatga
    14281 actatttggt catgaaaaag gctcgtacac cggggctgat caggttcatg cgggtaagtt
    14341 tgaagcggct gacggcggta cgttattcct ggatgaaatc gccaacatgt ctttatccgc
    14401 acaggccaag ctactgcgtg tgctacaaga gcacgttgtc tatcgtattg gcgcgactaa
    14461 acccattcct gtcaatgtgc gagtcctggc tgccagcaat gatgatctac tggacgcggt
    14521 agttaaaggg aaatttcgcg aggatctcta ttatcgattg aatgagtatg tcattcggat
    14581 tccgccgtta cgcaatcggc acgaggatat tctgtttttg tccaatcggt ttatcgccga
    14641 aaccagcgtc gaattgaaca aaacaccgcc tgatttgtcc gttgcggcta aagaaacact
    14701 gttacgttat ccatggccgg gtaacgtgcg agagttacgc gctgtcatcc gccgtgctgt
    14761 tttggtatcc gacaaaagag taggggttga tgatttaggc ctggtcatca aagaagatca
    14821 ctttccgcaa tcaacgaaaa atccgtatct gagaacgata cagaatttct gtctggaagg
    14881 ttcgtctctt aaagagatca ctcaactcaa tattgatcaa atagagcgaa ttatcattca
    14941 agacacatta gagaaaacag gcaataacaa ggcagaagca gcgcggcgtt tgaatattga
    15001 ctataaaacg ctgcactcta aactcaaaaa gatcaatgca gccgaggtaa acaatcatga
    15061 gtagtaaaaa aaaaatgatg tgacagggaa tgttgaagga attttgcgcg gtttgggcga
    15121 tctggtggaa aaattggttg aaaccggcga acagataaag cgtagcggtg cgttcgatat
    15181 tgacacgaac gacggtaaga atgccaaagc cgtgtatggt ttctccatca agatgggttt
    15241 ggatggaaac caggaaaatc gggtagaacc ttttggcaac atacgccggg atgagcaaac
    15301 cggggaagct acagtgcagg aagtgtctga accgttggtt gatgttatcg aagaaagtga
    15361 tcatgtgctg gtactggcag aaatgccggg ggtcgctgat gaagatgtgc aagtcgaatt
    15421 gaatggcgac attctgacgt tacactcgga aagaggcagt aaaaaatatc ataaagaaat
    15481 cgtgttgccg tgctcattcg atgacaaggc gatggaacgg tcttgccgta atggcatttt
    15541 ggaagtcaaa ttaggaaaat gaggcagtca tgtctgaaga actcaagctc aaggtcgccg
    15601 aggctctgcc aaaggatgct gggcgcggtt atgctcgctt agatccggcc gatatggcca
    15661 ggttaaatct ggctgtcggt gacatagtgc agttaaccag taaaaaaggc accgggattg
    15721 ctaaattgat gccgacttat cccgatatgc ggaataaagg catcgtacaa ctggatggtt
    15781 tgacgcggcg taataccagc ctgagtttag atgaaaaagt gcagattgaa cccgcttcct
    15841 gcaaacacgc cacacagatc gtcctcattc ccaccacgat tacgcccaac caacgtgacc
    15901 tgtattatat cggtagcctg ctcgatgggt tgcccgtaca gaaaggcgat ctgctgcgcg
    15961 cgcacctgtt tggcagtcgt tctgctgatt ttaaagtcga aagcaccata cctgatggtg
    16021 cggtactgat tgatccgacc acgacactgg tgattggcaa atcaaatgct gtcgggaatt
    16081 cgagtcatag cacacaacgt ttgtcgtatg aggatgttgg cggccttaaa aaccaggtgc
    16141 ggcgtattcg agaaatgatc gaattaccat tacgctatcc tgaggtcttt gaacgtttag
    16201 gtatagatgc ccccaaaggt gtactgctct ctggtccccc gggatgtggt aaaaccctca
    16261 tcgcccgcat tattgcgcaa gaaaccgatg cgcaattttt taccatcagc ggcccggaaa
    16321 ttgtgcataa gttttatggt gaaagtgaag cccacttacg caaaattttt gaggaagccg
    16381 gacgcaaagg gccaagtatc atttttctgg atgaaattga ctctattgca ccacaccgcg
    16441 acaaggtcgt cggtgatgta gaaaaacgta tcgttgcgca actgctcgca ttaatggatg
    16501 gtctcaagaa tcgaggcaaa gtcattgtca ttgccgcgac caatctacct aatgcgattg
    16561 atcccgcgtt gcgccgtccg ggacgctttg accgtgaaat cagcattcct attccagacc
    16621 gtgaaggtcg ccgcgagatt atcgaaattc atagcacggg aatgccgctg aatgctgatg
    16681 tcgatctgaa cgtgcttgcc gatatcactc acggttttgt cggtgccgat ctggaagcgt
    16741 tatgccgtga agccgcgatg agtgcgttac gccgtttact gcctgagatc gatttcagtt
    16801 cggcggagct accttatgat cgtctggccg aattaacggt gatgatggat gattttcgtg
    16861 ccgccttgtg tgaagtatca ccgtcagcga ttcgtgagct gttcgtcgat attcccgatg
    16921 tgcgttggga agatgtcggc ggtttggatg acgtacgccg tcgattgatc gaatccgtcg
    16981 agtggcctat caagtaccct gagctctatg aacaagcggg tgttaagccg cctaaaggtc
    17041 tgctgttagc tgggcctccc ggcgttggga aaacgctgat cgccaaagcc gttgctaatg
    17101 aaagtggcgt taatgtcatt tcagttaaag gtccggcgct gatgtcgcgt tatgtcggtg
    17161 attcggaaaa aggtgtccga gagctgtttc tcaaagctcg tcaggctgcg ccctgcatta
    17221 ttttccttga tgaagtcgat tctgtgattc ctgcccgtaa cgagggcgct atcgactcac
    17281 atgtcgcaga acgggtgttg agccaattct tatctgaaat ggatgggctg gaagaactca
    17341 aaggtgtttt cgttatgggg gcgaccaatc gtgcggactt gatcgatccc gcgatgctgc
    17401 gaccaggacg ttttgatgaa ataatcgaat tgggcctgcc cgacgaggac gcccgtcggc
    17461 agattctggc ggtgcattta cgtaataaac cgctgggtga caatattcat gctgacgatt
    17521 tggcagaacg ttgtgatggt gccagcggtg cagaattggc cgcggtgtgt aatcgtgccg
    17581 cattagcggc attgcgtcgt gcaatccagc agtctgaaga ggctgttctg agcccgtcaa
    17641 cggtaggaga aacccctgtg gcgctgacgg tacgcattga acagcatgat tttgctgaag
    17701 tgattgccga aatgtttggc gatgatgcat gacgatgaac acagaagcac agactgagca
    17761 ggccatttat ctgtatggcc tgacgttgcc cgatttagcc gcaccgccaa ttttgggcgt
    17821 tgataatcag catccgatta atacacatca atgtgccgga ttgaatgcgg tgattagccc
    17881 agtggcactc tccgatttca cgggtgaaaa gggtgaagac aatgtacaga atgtcacttg
    17941 gctaacgccg cgtatttgtc ggcatgcgca gattattgac agcctgatgg cccaaggtcc
    18001 ggtctatcct ttgcccttcg gcacgctgtt ttccagtcag aacgcgttgg aacaggaaat
    18061 gaaaagccgc gctactgacg tatttgtgtc gctacgtcgc ataaccggct gtcaggaatg
    18121 ggcactggaa gcgacactgg atcgtaaaca ggccgttgac gttttattca ccgaaggtct
    18181 ggactccggt cgcttttgcc tacccgaagc cataggccgc cgccatttgg aagagcagaa
    18241 attacgccgc aggttaacca ctgagttgag tgactggttg gcacatgctt tgactgccat
    18301 gcagaacgaa ttacatcctt tggtacgtga tttccgttca cgtcggttgt tggacgataa
    18361 aattttgcat tgggcgtatt tactcccggt ggaggacgtt gccgcgtttc aacagcaggt
    18421 tgctgacatt gttgaacgct atgaagctta cggttttagt tttcgcgtga caggcccgtg
    18481 ggcagcctac agtttttgcc aacctgacga atcatgagct tactactgta cggcattgtt
    18541 gccgaggata ctcaactggc gcttgaacca gacggttcgc cgcacgccgg tgaagagccg
    18601 atgcagcttg ttaaagcggc aacgttggcg gcgctggtca agccttgtga agcagacgtc
    18661 agtcgcgagc ccgcggcagc gtttgctttt ggccaacaga taatgcatgt tcatcaacag
    18721 accacgatta ttccgattcg ttatggttgt gtgttggcag atgaagatgc ggtaacacag
    18781 catttactca accatgaggc acattatcaa acgcaattgg ttgagctgga aaattgtgac
    18841 gaaatgggta ttcggttatc cctggcgtcg gctgaggata atgccgttac tacgccgcaa
    18901 gcgagtggtc tggactattt gcggagtcga aaactcgctt atgccgtacc tgaacacgct
    18961 gaacggcagg ctgcactact gaataatgcg tttacggggt tgtatcgccg acactgcgcg
    19021 gaaatcagta tgtttaatgg tcagcggact tacctgttga gctatctggt gccacgaacc
    19081 gggttacagg cgtttcgcga ccaatttaat actctggcca acaacatgac ggatataggg
    19141 gttatcagtg ggccttggcc tccctataac ttcgccagct aatttgttaa cgacaggcta
    19201 ccatgtcaga taatcctttt gaacagcagt cagaaaacca ctttgaactt ctttacaaca
    19261 tgctattgga tgcaattcct tcttccgtgt taatggtgga tgagaaactg catgtggttt
    19321 cagtcaatca gaatttttta atcaagggtc atcgcggcaa cccggataca attggtcgcc
    19381 cgttatcgga cgtctttccg agcgtcattt tggaaaatac gggtcttgaa gcacatatcc
    19441 gggatgtatt ccgtagcgga ttacccgtta atggtcagaa aatgacctac cgtgcgccag
    19501 gcgtaccgat tcggatttat tactattcta ttctacccat ccccattaac cagattatgc
    19561 tgttcatgga ggatgtcacc gagcaggttc gcctgtctga agaagtccat cgagtagaac
    19621 ggcacctcgc ttcagttgta gaaaacgcca gcgacattgt tctttcgacc gatattgaag
    19681 gatgcatatt gacgtggaat tcagcggcgg aaaagctgtc aggtttaccc acgcatgaag
    19741 tctatgggcg atactttttt gagttctgcg ataccaacca aaaggaggcg gtgcgtctga
    19801 ttttcatgcg tatgaagaac agcaatgaat cacatacgac tgaatttcat ttattaacgc
    19861 ctaacaatgg tggcagcata ttgatttcct gggtattttc accaatgaag gatgactatg
    19921 ggcaaacggt gggcaccgta gttgtcggtc gtgatttggc tgaacgtcgg aaattggaac
    19981 tgcaattacg tcaatcacag aagttgaccg cgttgggcgt catggcgggc ggtatcgctc
    20041 atgaaattcg taatcctctt gctgtttgtt cttcagcggc gcaatttttg ctggaagacg
    20101 agattacggc tgaatttcat aaagagtgtt caagaaaaat acagactaac attcaaaaag
    20161 cctcggccat cattgagaat ttactccgtt ttgcccgttc ttccctcgat accgaaatgg
    20221 tagaggtcaa tctgatcacg gtgctgaaag aaactatcga cctgatcgcc aaccaggcca
    20281 aagtgcagaa aattcagatc ctgttgccgt tgcaaaaagg aaatatattg gtgtcaggtg
    20341 tgtcgggctt actgcaacag gtgttcatga atatttttct taatgccatt aatgcgatgc
    20401 cggaaggcgg tgtgttaagt attgcggtag agacctttga agaccgggtt attgtgcgta
    20461 tttctgatac tggcgtaggc attcccgccg gcgagttgga taaaattttc gatccgttcc
    20521 acacgtcttc acccgtaggc caaggtactg gtttgggttt gtccatttgt tacagcatta
    20581 tccaacagca ccttggttcc atacaggcag agagttgtct gggtgaaggt tccactatta
    20641 ttgttacctt acctatactc taaaatgatg aacgcaccca ttctgatagt tgatgatgaa
    20701 cctgacatgt gttgggccat ttcccatatt attcaatcac aaggcatgag tgtcattacc
    20761 gcttacagtg gggaagatgc ttgcgttaag ctgatgaccg gcaatttttc ctttgtgttt
    20821 ttagatgcca agttaccgga tatggacggt ctggaagtgg cgcgtcgagc cagtaccttg
    20881 ctagagcgaa gaccgcgtgt agtgctggtt tccggttatc actattacga tgatccggtt
    20941 attcgccatg ctatcgttga aggcattatc tgcggttttc tggcgaagcc ttttaccaat
    21001 aaagagctgc tgaaaacatt acagacacta tcacagccga gcgagtagtt gcgtcgctag
    21061 cttaatcaac gctctgcttc ttcgggggga attactgaca cgctgcacct taaaacgggc
    21121 attatgacga tagtgctcgt tttaaggtgc aaatggtgtt ttatcgttag cgtgatgtga
    21181 tgagatgtgc agcttctttt ttcgatacat gagcggagtc atcttcattt cttttcgaaa
    21241 ctgattaata aaatagcttg tactattaaa ccctacttga taggcgactt ccgtcacatt
    21301 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
    21361 agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag
    21421 tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc
    21481 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
    21541 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
    21601 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt
    21661 aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt
    21721 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
    21781 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
    21841 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
    21901 tgctgaagat cagttgggtg cagcaaacta ttaactggcg aactacttac tctagcttcc
    21961 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    22021 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    22081 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    22141 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    22201 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    22261 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
    22321 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
    22381 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
    22441 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
    22501 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
    22561 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
    22621 agggattttg ccgatttcgg catattggtt aaaaaatgag ctgatttaac aaaaatttaa
    22681 cgcgaatttt aacaaaatat taacgtttac aatttaaaag gatctaggtg aagatccttt
    22741 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    22801 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    22861 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    22921 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
    22981 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    23041 tgctaatcct gttaccagtc aggcatttga gaagcacacg gtcacactgc ttccggtagt
    23101 caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc
    23161 gacgaccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt atcacttatt
    23221 caggcgtagc aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg
    23281 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
    23341 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
    23401 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
    23461 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
    23521 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
    23581 agaaactgcc ggaaatcgtc gtggtattca caccagagcg atgaaaacgt ttcagtttgc
    23641 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
    23701 attgccatac ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
    23761 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
    23821 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
    23881 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
    23941 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
    24001 tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc
    24061 cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc
    24121 gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt actgatttag
    24181 tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg
    24241 ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctagcg
    24301 gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg
    24361 gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg
    24421 cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt
    24481 acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag
    24541 ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg
    24601 acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    24661 tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct
    24721 gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct
    24781 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta
    24841 actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg
    24901 gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg
    24961 acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct
    25021 cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt
    25081 acgcgcagac caaaacgatc tcaa
//
