LOCUS       sequence-472145-        2751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Terminator; BEVA2.0 T15m terminator with DF extension for CIDAR 
            MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly 
            of PCR amplicon into POGG006, SpR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Geddes BA, Williamson R, Schumacher J, Ardi A, Levin G, Cervenka E, 
            Huang R, diCenzo G
  TITLE     BEVA2.0: modular assembly of golden gate-compatible vectors with 
            expanded utility for genetic engineering.
  JOURNAL   Can J Microbiol. 2025 Jan 1;71:1-13. doi: 10.1139/cjm-2024-0246.
  PUBMED    39919271
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Can J 
            Microbiol."; date: "2025-01-1"; volume: "71:1-13. doi"; pages: " 
            10.1139/cjm-2024-0246"
COMMENT     Sequence Label: pNDGG039
FEATURES             Location/Qualifiers
     source          1..2751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      500..594
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(741..758)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(851..1439)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(931..950)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(2624..2728)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggcctaggc gcgccacact ctgtgcgtct catgcctgcc cggccgcttc tagagactag
      421 tgggtctcaa ggtccaatta ttgaacaccc ttcggggtgt ttttttgttt ctggtctgcc
      481 cgcttgagac cgggacttgg actcctgttg atagatccag taatgacctc agaactccat
      541 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaggc
      601 aatgagacgc acgaagtgat taattaactg gcctcatggg ccttcacttc actgcccgct
      661 ttccagtcgg gaaacctgtc gtgccagctg cattaacatg gtcatagctg tttccttgcg
      721 tattgggcgc tctccgcttc ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag
      781 cctggggtgc ctaatgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      841 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      901 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      961 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1021 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1081 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1141 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1201 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1261 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1321 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1381 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1441 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1501 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1561 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt tattatttac
     1621 ccaccacttt ggtaatttcg cctttcacgt aatgcacaaa ttcttccagc tgatccgcac
     1681 ggctggccag acgatcttct tcctggccca gatacgcctg acgcgcttcc agaatcaccg
     1741 gctgatactg cgccggcaga cgttccatgg cccaatccgc cgccacatct ttcggcgcaa
     1801 ttttgccggt caccgcgcta taccaaatac ggctcagggt cagcaccacg ttgcgttcat
     1861 cacccgccca atccggcggg ctgttccaca gggtcagggt ttcgttcagc gcttcaaaca
     1921 ggtcctgttc cggcaccgga tcaaacagtt cttccgctgc cggaccaacc agcgccacgc
     1981 tatgttcacg cgctttggtc agcagaatgg ccagatcgat atcaatggtc gccggttcaa
     2041 aaatgccggc cagaatatcg ttacgctgcc attcgccaaa ctgcagctca cgtttcgccg
     2101 gataacgcca cggaataatg tcatcatgca ccacgatggt cacttccacc gcacgcagaa
     2161 tttcgctttc acccgggctc gcgctggttt ccagcagatc gttaatcagc gcacgacggg
     2221 tggtttcatc cagacgcacg gtcacggtca ccagcagatc aatatcgcta tgcggtttca
     2281 ggccgccatc caccgcgctg ccatacagat gcacggccag cagggtcggt tccagatgac
     2341 gttcaatcac gcccaccact tcagacagct gggtgctcac ttccgcaatc accgcttcac
     2401 gcataatgtt cagtttggtt ttcggacggc tgccctgctg ggtcacatcg ttgctgctcc
     2461 acagcatcag gcaacggccc accgcataac gcgcctgctg tttgctcgca cgcggcatgc
     2521 tctgcacgcc aaaaaagcaa tcatagcacg ccataaacac cgccaccgca ccattaccgc
     2581 cgctacgttc ggtcagggta cggctccaat tacggctacg catactcttc ctttttcaat
     2641 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     2701 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
