LOCUS       sequence-472163-        4370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_MERS-CoV-nsp4
FEATURES             Location/Qualifiers
     source          1..4370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1681..1780)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1797..1816)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1798..1816)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1821..1837)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1821..1837)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2268..3059
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3152..3740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3641..3660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3894..3911
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4070..4097)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4189..4275)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4324..4346
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4338..4355
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4339..4355
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatccg ctcctacatg gtttaatgcg ttgcgtgact ttacgttaaa gggttatgtt
      181 cttgctacca ttattgtgtt tctgtgtgct gtactgatgt atttgtgttt acctacattt
      241 tctatggcac ctgttgaatt ttatgaagac cgcatcttgg actttaaagt tcttgataat
      301 ggtatcatta gggatgtaaa tcctgatgat aagtgctttg ctaataagca ccggtccttc
      361 acacaatggt atcatgagca tgttggtggt gtctatgaca actctatcac atgcccattg
      421 acagttgcag taattgctgg agttgctggt gctcgcattc cagacgtacc tactacattg
      481 gcttgggtga acaatcagat aattttcttt gtttctcgag tctttgctaa tacaggcagt
      541 gtttgctaca ctcctataga tgagataccc tataagagtt tctctgatag tggttgcatt
      601 cttccatctg agtgcactat gtttagggat gcagagggcc gtatgacacc atactgccat
      661 gatcctactg ttttgcctgg ggcttttgcg tacagtcaga tgaggcctca tgttcgttac
      721 gacttgtatg atggtaacat gtttattaaa tttcctgaag tagtatttga aagtacactt
      781 aggattacta gaactctgtc aactcagtac tgccggttcg gtagttgtga gtatgcacaa
      841 gagggtgttt gtattaccac aaatggctcg tgggccattt ttaatgacca ccatcttaat
      901 agacctggtg tctattgtgg ctctgatttt attgacattg tcaggcggtt agcagtatca
      961 ctgttccagc ctattactta tttccaattg actacctcat tggtcttggg tataggtttg
     1021 tgtgcgttcc tgactttgct cttctattat attaataaag taaaacgtgc ttttgcagat
     1081 tacacccagt gtgctgtaat tgctgttgtt gctgctgttc ttaatagctt gtgcatctgc
     1141 tttgttacct ctataccatt gtgtatagta ccttacactg cattgtacta ttatgctaca
     1201 ttctatttta ctaatgagcc tgcatttatt atgcatgttt cttggtacat tatgttcggg
     1261 cctatcgttc ccatatggat gacctgcgtc tatacagttg caatgtgctt tagacacttc
     1321 ttctgggttt tagcttattt tagtaagaaa catgtagaag tttttactga tggtaagctt
     1381 aattgtagtt tccaggacgc tgcctctaat atctttgtta ttaacaagga cacttatgca
     1441 gctcttagaa actctttaac taatgatgcc tattcacgat ttttggggtt gtttaacaag
     1501 tataagtact tctctggtgc tatggaaaca gccgcttatc gtgaagctgc agcatgtcat
     1561 cttgctaaag ccttacaaac atacagcgag actggtagtg atcttcttta ccaaccaccc
     1621 aactgtagca taacctctgg cgtgttgcaa cttaattaag acgcgatcgc ggcctcatgg
     1681 gcccagcttt cttgtacaaa gttggcatta taaaaaataa ttgctcatca atttgttgca
     1741 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
     1801 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     1861 atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc
     1921 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     1981 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     2041 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     2101 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     2161 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     2221 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     2281 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     2341 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     2401 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     2461 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     2521 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     2581 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     2641 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     2701 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     2761 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     2821 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     2881 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     2941 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     3001 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     3061 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     3121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3781 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3841 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3901 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3961 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     4021 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
     4081 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     4141 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
     4201 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
     4261 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt
     4321 tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
