LOCUS       sequence-472167-        3503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_MERS-CoV-Membrane
FEATURES             Location/Qualifiers
     source          1..3503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(387..486)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(502..519)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(502..518)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(511..533)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      582..668
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      760..787
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(946..963)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1117..1705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1197..1216)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1798..2589)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     3020..3036
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3020..3036
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3041..3060
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3041..3059
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3077..3176
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gttcaaaagg caattagtct caggattgaa tgaccaccat gatccagttc tcataaacag
       61 ccggatactc tgcacaaagt aggaaatcca catcatagct gaaacagctg ctacaatgcc
      121 agagattatc tgggaagcta gatcaattgg ataaacggcg ctaaatattg atagcgccat
      181 ggaagatggc cataggagcc ataaaacaaa cattttaaag acatagacag tcatactacg
      241 ggatgggtat ccatactgta gtacgatagt aattaagaga aagatcaggg accatgcaaa
      301 gttccagtct ttaataatgg caataatctg cgcctcagtg agttgcgtca tattagagga
      361 tcccatggat cctggccttg acggcccttc tgcttttttg tacaaagttg gcattataaa
      421 aaagcattgc tcatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
      481 tatttggggc ccgagcttaa gactggccgt cgttttacaa cgtcgtgact gggaaaacat
      541 ccatgctagc gttaacgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
      601 agtcggaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
      661 ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca acggcccgga gggtggcggg
      721 caggacgccc gccataaact gccaggcatc aaactaagca gaaggccatc ctgacggatg
      781 gcctttttgc gtttctacaa actcttcctg gctagcggta cgcgtattaa ttgcgttgcg
      841 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      901 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      961 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1021 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1081 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1141 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1201 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1261 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1321 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1381 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1441 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1501 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1561 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1621 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1681 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1741 tcagtggaac gacgcgtaac tcacgttaag ggattttggt catgggtggc tcgagggtta
     1801 tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc
     1861 tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat
     1921 gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg
     1981 cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg
     2041 ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc
     2101 aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc
     2161 aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg
     2221 ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt
     2281 agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg
     2341 gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg
     2401 ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg
     2461 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
     2521 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
     2581 ttccctcata atgtttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct
     2641 gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg
     2701 catagactgt accccaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     2761 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     2821 ttacagctta cgaaccgaac aggcttatgt ccactgggtt cgtgccttca tccgtttcca
     2881 cggtgtgcgt cacccggcaa ccttgggtag cagcgaagtc gaggcatttc tgtcctggct
     2941 ggtctagagg aggcatgatg atatattttt atcttgtgca atgtaacatc agagattttg
     3001 agacacgggc cagagctgcc aggaaacagc tatgaccatg taatacgact cactataggg
     3061 gatatcagct ggatggcaaa taatgatttt attttgactg atagtgacct gttcgttgca
     3121 acaaattgat gagcaattat tttttataat gccaactttg tacaagaaag ctgggcccat
     3181 gaggccgcga tcgcgtctta attaagagct cgaagcaatg caagttcaat atccgccgta
     3241 ataggcggac tcctgtaatt acctgcctta tatctatggt aaatggcaac gccggaatta
     3301 gttccgtagc tttgccgctt caccattttt aaagcaatca gcacattggg tttggccacg
     3361 gtgacttcat taggaagtct gtcgtagtca caagcaccga aatgcatgcc agccattttg
     3421 aggtggccat tggttacaac agcagttaca ctggtagagt cctctacgag tggacgtacg
     3481 acagttgtac caccaaatgg aac
//
