LOCUS       sequence-472170-        5648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_MERS-CoV-nsp12
FEATURES             Location/Qualifiers
     source          1..5648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2959..3058)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3075..3094)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3076..3094)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3099..3115)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3099..3115)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3546..4337
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4430..5018
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4919..4938
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5172..5189
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5348..5375)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5467..5553)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5602..5624
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5616..5633
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5617..5633
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatcct ctaaagattc caatttttta aaccgagtcc ggggttctat tgtaaatgcc
      181 cgaatagaac cctgttcaag tggtttgtcc actgatgtcg tctttagggc atttgacatc
      241 tgcaactata aggctaaggt tgctggtatt ggaaaatact acaagactaa tacttgtagg
      301 tttgtagaat tagatgacca agggcatcat ttagactcct attttgtcgt taagaggcat
      361 actatggaga attatgaact agagaagcac tgttacgact tgttacgtga ctgtgatgct
      421 gtagctcccc atgatttctt catctttgat gtagacaaag ttaaaacacc tcatattgta
      481 cgtcagcgtt taactgagta cactatgatg gatcttgtat atgccctgag gcactttgat
      541 caaaatagcg aagtgcttaa ggctatctta gtgaagtatg gttgctgtga tgttacctac
      601 tttgaaaata aactctggtt tgattttgtt gaaaatccca gtgttattgg tgtttatcat
      661 aaacttggag aacgtgtacg ccaagctatc ttaaacactg ttaaattttg tgaccacatg
      721 gtcaaggctg gtttagtcgg tgtgctcaca ctagacaacc aggaccttaa tggcaagtgg
      781 tatgattttg gtgacttcgt aatcactcaa cctggttcag gagtagctat agttgatagc
      841 tactattctt atttgatgcc tgtgctctca atgaccgatt gtctggccgc tgagacacat
      901 agggattgtg attttaataa accactcatt gagtggccac ttactgagta tgattttact
      961 gattataagg tacaactctt tgagaagtac tttaaatatt gggatcagac gtatcacgca
     1021 aattgcgtta attgtactga tgaccgttgt gtgttacatt gtgctaattt caatgtattg
     1081 tttgctatga ccatgcctaa gacttgtttc ggacccatag tccgaaagat ctttgttgat
     1141 ggcgtgccat ttgtagtatc ttgtggttat cactacaaag aattaggttt agtcatgaat
     1201 atggatgtta gtctccatag acataggctc tctcttaagg agttgatgat gtatgccgct
     1261 gatccagcca tgcacattgc ctcctctaac gcttttcttg atttgaggac atcatgtttt
     1321 agtgtcgctg cacttacaac tggtttgact tttcaaactg tgcggcctgg caattttaac
     1381 caagacttct atgatttcgt ggtatctaaa ggtttcttta aggagggctc ttcagtgacg
     1441 ctcaaacatt ttttctttgc tcaagatggt aatgctgcta ttacagatta taattactat
     1501 tcttataatc tgcctactat gtgtgacatc aaacaaatgt tgttctgcat ggaagttgta
     1561 aacaagtact tcgaaatcta tgacggtggt tgtcttaatg cttctgaagt ggttgttaat
     1621 aatttagaca agagtgctgg ccatcctttt aataagtttg gcaaagctcg tgtctattat
     1681 gagagcatgt cttaccagga gcaagatgaa ctttttgcca tgacaaagcg taacgtcatt
     1741 cctaccatga ctcaaatgaa tctaaaatat gctattagtg ctaagaatag agctcgcact
     1801 gttgcaggcg tgtccatact tagcacaatg actaatcgcc agtaccatca gaaaatgctt
     1861 aagtccatgg ctgcaactcg tggagcgact tgcgtcattg gtactacaaa gttctacggt
     1921 ggctgggatt tcatgcttaa aacattgtac aaagatgttg ataatccgca tcttatgggt
     1981 tgggattacc ctaagtgtga tagagctatg cctaatatgt gtagaatctt cgcttcactc
     2041 atattagctc gtaaacatgg cacttgttgt actacaaggg acagatttta tcgcttggca
     2101 aatgagtgtg ctcaggtgct aagcgaatat gttctatgtg gtggtggtta ctacgtcaaa
     2161 cctggaggta ccagtagcgg agatgccacc actgcatatg ccaatagtgt ctttaacatt
     2221 ttgcaggcga caactgctaa tgtcagtgca cttatgggtg ctaatggcaa caagattgtt
     2281 gacaaagaag ttaaagacat gcagtttgat ttgtatgtca atgtttacag gagcactagc
     2341 ccagacccca aatttgttga taaatactat gcttttctta ataagcactt ttctatgatg
     2401 atactgtctg atgacggtgt cgtttgctat aatagtgatt atgcagctaa gggttacatt
     2461 gctggaatac agaattttaa ggaaacgctg tattatcaga acaatgtctt tatgtctgaa
     2521 gctaaatgct gggtggaaac cgatctgaag aaagggccac atgaattctg ttcacagcat
     2581 acgctttata ttaaggatgg cgacgatggt tacttccttc cttatccaga cccttcaaga
     2641 attttgtctg ccggttgctt tgtagatgat atcgttaaga ctgacggtac actcatggta
     2701 gagcggtttg tgtctttggc tatagatgct taccctctca caaagcatga agatatagaa
     2761 taccagaatg tattctgggt ctacttacag tatatagaaa aactgtataa agaccttaca
     2821 ggacacatgc ttgacagtta ttctgtcatg ctatgtggtg ataattctgc taagttttgg
     2881 gaagaggcat tctatagaga tctctatagt tcgcctacca ctttgcagct taattaagac
     2941 gcgatcgcgg cctcatgggc ccagctttct tgtacaaagt tggcattata aaaaataatt
     3001 gctcatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     3061 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     3121 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     3181 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     3241 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     3301 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     3361 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     3421 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     3481 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     3541 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     3601 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     3661 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     3721 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     3781 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     3841 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     3901 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     3961 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     4021 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     4081 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     4141 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     4201 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     4261 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     4321 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     4381 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4441 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4501 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4561 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4621 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4681 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4741 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4801 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4861 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4921 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4981 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5041 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5101 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5161 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     5221 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     5281 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     5341 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     5401 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     5461 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     5521 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt
     5581 taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc agtcttaagc
     5641 tcgggccc
//
