LOCUS       sequence-47218-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3140-02
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..71
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        128..471
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 ggatccccgg gcttaagatc ggtgacggtg ctggtttaat taactatcca tatgatgttc
       61 ctgactatgc ttaaggcgcg ccagatctgt ttagcttgcc tcgtccccgc cgggtcaccc
      121 ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg
      181 ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc
      241 atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcggac
      301 ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct
      361 gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt
      421 taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac catgggtaaa
      481 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
      541 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
      601 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
      661 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
      721 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
      781 ctgcctgaaa ccgaactgcc cgctgttctg cagcc
//
