LOCUS       sequence-47219-e         630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Ikeda M, Kawata A, Nishikawa M, Tateishi Y, Yamaguchi M, Nakagawa K,
            Hirabayashi S, Bao Y, Hidaka S, Hirata Y, Hata Y
  TITLE     Hippo pathway-dependent and -independent roles of RASSF6.
  JOURNAL   Sci Signal. 2009 Sep 29;2(90):ra59.
  PUBMED    19797269
REFERENCE   2  (bases 1 to 630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2009-09-29"; volume: "2(90)"; pages: "ra59"
COMMENT     Sequence Label: pClneoFH human RASSF1A
FEATURES             Location/Qualifiers
     source          1..630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             61..84
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             91..108
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attacagctc ttaaggctag agtacttaat acgactcact ataggctagc cggggccatg
       61 gactacaaag acgatgacga caaggaattg caccatcatc atcatcatga attctcgggg
      121 gagcctgagc tcattgagct gcgggagctg gcacccgctg ggcgcgctgg gaagggccgc
      181 acccggctgg agcgtgccaa cgcgctgcgc atcgcgcggg gcaccgcgtg caaccccaca
      241 cggcagctgg tccctggccg tggccaccgc ttccagcccg cggggcccgc cacgcacacg
      301 tggtgcgacc tctgtggcga cttcatctgg ggcgtcgtgc gcaaaggcct gcagtgcgcg
      361 cattgcaagt tcacctgcca ctaccgctgc cgcgcgctcg tctgcctgga ctgttgcggg
      421 ccccgggacc tgggctggga acccgcggtg gagcgggaca cgaacgtgga cgagcctgtg
      481 gagtgggaga cacctgacct ttctcaagct gagattgagc agaagatcaa ggagtacaat
      541 gcccagatca acagcaacct cttcatgagc ttgaacaagg acggttctta cacaggcttc
      601 atcaaggttc agctgaagct ggtgcgccct
//
