LOCUS       sequence-472199-        3140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-1-ORF9b
FEATURES             Location/Qualifiers
     source          1..3140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(451..550)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(567..586)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(568..586)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(591..607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(591..607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1038..1829
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1922..2510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2411..2430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2664..2681
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2840..2867)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2959..3045)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3094..3116
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3108..3125
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3109..3125
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatccg accccaatca aaccaacgta gtgccccccg cattacattt ggtggaccca
      181 cagattcaac tgacaataac cagaatggag gacgcaatgg ggcaaggcca aaacagcgcc
      241 gaccccaagg tttacccaat aatactgcgt cttggttcac agctctcact cagcatggca
      301 aggaggaact tagattccct cgaggccagg gcgttccaat caacaccaat agtggtccag
      361 atgaccaaat tggctactac cgaagagcta cccgacgagt tcgtggtggt gacggcaaaa
      421 cttaattaag acgcgatcgc ggcctcatgg gcccagcttt cttgtacaaa gttggcatta
      481 taaaaaataa ttgctcatca atttgttgca acgaacaggt cactatcagt caaaataaaa
      541 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
      601 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
      661 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
      721 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
      781 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
      841 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
      901 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
      961 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     1021 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     1081 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     1141 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     1201 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     1261 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     1321 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     1381 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     1441 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     1501 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     1561 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     1621 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     1681 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     1741 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     1801 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     1861 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1921 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1981 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2041 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     2101 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2161 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2221 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2281 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2341 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2401 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2461 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2521 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2581 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2641 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     2701 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     2761 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     2821 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     2881 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     2941 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     3001 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     3061 ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt aaaacgacgg
     3121 ccagtcttaa gctcgggccc
//
