LOCUS       sequence-472207-        9907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mHEL and OT2-peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Guo Z, et al.
  TITLE     Sentinel Node B Cells Drive Epitope Spreading and Anti-Tumor T Cell 
            Immunity by Recognition and Presentation of Extracellular 
            Vesicle-Linked Antigens
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p67.uni.pA.OTIIp.mHEL.flag.mCMV.PGK.CD63GFP.WPRE
FEATURES             Location/Qualifiers
     source          1..9907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..715
                     /codon_start=1
                     /product="human CD63 tetraspanin"
                     /label=CD63
                     /note="enriched in intraluminal vesicles of late endosomes 
                     (multivesicular bodies) and in exosomes"
                     /translation="MAVEGGMKCVKFLLYVLLLAFCACAVGLIAVGVGAQLVLSQTIIQ
                     GATPGSLLPVVIIAVGVFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAAAIAG
                     YVFRDKVMSEFNNNFRQQMENYPKNNHTASILDRMQADFKCCGAANYTDWEKIPSMSKN
                     RVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEVLGIV
                     FACCLVKSIRSGYEVM"
     regulatory      755..764
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(761..763,764..766,767..1480)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(761..763,764..766,767..1477)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(806..827)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1067..1086)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1414..1435
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1549..1569)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1967..1978)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2127..2360
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2432..2553
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2593..2728
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2748..2767)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2749..2767)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2777..2794)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2777..2793)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2786..2808)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2935..3390
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3022..3041)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3232..3253
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3416..3520
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3521..3589,3590..4381)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3739..3758)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4552..5140
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5041..5060
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5294..5311
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5428..5449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5464..5469,5470..5487,5488..5494)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5502..5518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5507..5529
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5526..5542
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5526..5542
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5561..5581
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5563..5581
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        5682..6061
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6062..6265
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             6280..6460
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6507..6632
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7125..7358
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7543..7587
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7854..7971
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    8108..8242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(9036..9059)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(9318..9356)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        9384..9894
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9813..9831
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 catggcggtg gaaggaggaa tgaaatgtgt gaagttcttg ctctacgtcc tcctgctggc
       61 cttttgcgcc tgtgcagtgg gactgattgc cgtgggtgtc ggggcacagc ttgtcctgag
      121 tcagaccata atccaggggg ctacccctgg ctctctgttg ccagtggtca tcatcgcagt
      181 gggtgtcttc ctcttcctgg tggcttttgt gggctgctgc ggggcctgca aggagaacta
      241 ttgtcttatg atcacgtttg ccatctttct gtctcttatc atgttggtgg aggtggccgc
      301 agccattgct ggctatgtgt ttagagataa ggtgatgtca gagtttaata acaacttccg
      361 gcagcagatg gagaattacc cgaaaaacaa ccacactgct tcgatcctgg acaggatgca
      421 ggcagatttt aagtgctgtg gggctgctaa ctacacagat tgggagaaaa tcccttccat
      481 gtcgaagaac cgagtccccg actcctgctg cattaatgtt actgtgggct gtgggattaa
      541 tttcaacgag aaggcgatcc ataaggaggg ctgtgtggag aagattgggg gctggctgag
      601 gaaaaatgtg ctggtggtag ctgcagcagc ccttggaatt gcttttgtcg aggttttggg
      661 aattgtcttt gcctgctgcc tcgtgaagag tatcagaagt ggctacgagg tgatgatgga
      721 gagcgacgag agcggcctgc ccgccatgcc ggtcgccacc atggtgagca agggcgagga
      781 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      841 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      901 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
      961 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     1021 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1081 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1141 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1201 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1261 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1321 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     1381 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1441 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg tcgacaatca
     1501 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     1561 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     1621 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     1681 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     1741 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     1801 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     1861 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     1921 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     1981 agcggacctt ccttcccgcg gcctgctgcc ggctctagag cctcttccgc gtcttcgcct
     2041 tcccgggtcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     2101 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     2161 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     2221 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     2281 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     2341 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     2401 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     2461 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     2521 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     2581 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     2641 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     2701 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     2761 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     2821 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     2881 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     2941 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3001 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3061 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3121 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     3181 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3241 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3301 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3361 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     3421 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3481 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3541 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3601 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3661 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3721 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3781 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3841 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3901 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3961 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4021 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4081 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4141 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4201 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4261 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4321 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4381 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4441 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4501 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4561 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4621 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4681 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4741 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4801 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4861 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4921 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4981 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5041 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5101 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5161 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5221 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5281 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     5341 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     5401 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     5461 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     5521 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     5581 ggaacaaaag ctggagctgc aagcttggcc attgcatacg ttgtatccat atcataatat
     5641 gtacatttat attggctcat gtccaacatt accgccatgt tgacattgat tattgactag
     5701 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     5761 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     5821 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     5881 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     5941 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     6001 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     6061 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     6121 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     6181 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     6241 gtgggaggtc tatataagca gagctcgttt agtgaaccgg ggtctctctg gttagaccag
     6301 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6361 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6421 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     6481 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     6541 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     6601 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     6661 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     6721 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     6781 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     6841 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     6901 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     6961 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     7021 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     7081 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     7141 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     7201 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     7261 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     7321 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     7381 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     7441 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     7501 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     7561 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     7621 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     7681 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     7741 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     7801 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa
     7861 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     7921 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac
     7981 gagactagcc tcgagagatc tgatcataat cagccatacc acatttgtag aggttttact
     8041 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     8101 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaggcaata gcatcacaaa
     8161 tttcacaaat aaggcatttt tttcactgca ttctagtttt ggtttgtcca aactcatcaa
     8221 tgtatcttat catgtctgga tctcaaatcc ctcggaagct gcgcctgtca tcgaattcct
     8281 gcagcccggt gcatgactaa gctagcttag gcaaggcgtc cagcttcatt aatttcagcg
     8341 tgggcagcat ggactgcttg tgagatactc cctgaggcaa gtgaatgggg gtcatgaacc
     8401 attaccttgc aatctggaag ggacaagtcg ctagtctgag accctggggc aagagcgtag
     8461 tcaccgcctt tgccccctgt gttcctcctc ctcatcttca tcacgaatgc cacaactgct
     8521 ccggttacta tagcagctcc caagaccacg agtacggcga cagtagccat attagagact
     8581 gtgctggggg gaggttccca gcgcagggtc agaggttcgg gcaacccctg gtgatcttcc
     8641 agtctacaac ctcgaatcca ggcttggaca tctgtgccct tgcacctatt tcgccaagcg
     8701 acccaggcgt tcataccgtt cccatcggac actatctttt tggcacaatt cacgctggcg
     8761 gtaatgtcgg aactgagcaa agctgagcag gggatattac aaaggttgcg ggagcctggt
     8821 gttcgcccat cgttgcacca ccatcgggag ttgatctgca aaatgccata gtcagtacta
     8881 ccatctgtgt ttcgattggt ggcctgagta ttgaagttgg attcaaattt ggcggcgcac
     8941 acccagttcc ccaaggaata ccctctgtag ttatcgaggc cgtgtctttt cattgctgct
     9001 gccagctcgc atcggccgaa taccttgccg gaccctttat catcgtcgtc cttgtagtcc
     9061 ccgagggcag ccagtggcag aaagcaaagt acgagtatca gcaagctgcg catggtggcg
     9121 catgcagcgc tcgacttcca gctcggtccg ctttgcggac tgatggggct gcgctgcgct
     9181 gcgctccagc gccccccctg cccgccggag ctggccgcgg cccgaattcc gcggaggctg
     9241 gatcggtccc ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc
     9301 tgacggttca ctaaacgagc tctgcttata taggcctccc accgtacacg cctaccctcg
     9361 agaagcttga tatcgaattc cacggggttg gggttgcgcc ttttccaagg cagccctggg
     9421 tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct
     9481 gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt
     9541 gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc
     9601 gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga
     9661 cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc
     9721 tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg
     9781 gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag
     9841 cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat
     9901 ccaccgg
//
