LOCUS       sequence-472259-       16812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression in Nicotiana benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16812)
  AUTHORS   Sung YC, Li Y, Bernasconi Z, Baik S, Asuke S, Keller B, Fahima T, 
            Coaker G
  TITLE     Wheat tandem kinase RWT4 directly binds a fungal effector to 
            activate defense.
  JOURNAL   Nat Genet. 2025 Apr 14. doi: 10.1038/s41588-025-02162-w.
  PUBMED    40229601
REFERENCE   2  (bases 1 to 16812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2025 Apr 14. doi: 10.1038/s41588-025-02162-w."
COMMENT     Sequence Label: pTA7001-RWT4Ca_noKDup
FEATURES             Location/Qualifiers
     source          1..16812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..642
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  1335..2102
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             2294..3088
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             3387..4535
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    6131..6155
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(6402..6423)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6614..6633
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6758..6780
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6772..6789
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6773..6789
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6999..7045
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    7077..7101
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9432..9497
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(9501..9525)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9533..9598
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      complement(10124..10376)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(11491..11674)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(11527..11706)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             complement(12998..13231)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        complement(13493..13838)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    14353..14377
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             14842..15492
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(15680..15697)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(15931..15950)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            16173..16282
                     /note="incP origin of transfer"
     CDS             16315..16686
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 gcgcctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agcgctggca
      721 gtccttgcca ttgccgggat cggggcagta acgggatggg cgatcagccc gagcgcgacg
      781 cccggaagca ttgacgtgcc gcaggtgctg gcatcgacat tcagcgacca ggtgccgggc
      841 agtgagggcg gcggcctggg tggcggcctg cccttcactt cggccgtcgg ggcattcacg
      901 gacttcatgg cggggccggc aatttttacc ttgggcattc ttggcatagt ggtcgcgggt
      961 gccgtgctcg tgttcggggg tgcgataaac ccagcgaacc atttgaggtg ataggtaaga
     1021 ttataccgag gtatgaaaac gagaattgga cctttacaga attactctat gaagcgccat
     1081 atttaaaaag ctaccaagac gaagaggatg aagaggatga ggaggcagat tgccttgaat
     1141 atattgacaa tactgataag ataatatatc ttttatatag aagatatcgc cgtatgtaag
     1201 gatttcaggg ggcaaggcat aggcagcgcg cttatcaata tatctataga atgggcaaag
     1261 cataaaaact tgcatggact aatgcttgaa acccaggaca ataaccttat agcttgtaaa
     1321 ttctatcata attgggtaat gactccaact tattgatagt gttttatgtt cagataatgc
     1381 ccgatgactt tgtcatgcag ctccaccgat tttgagaacg acagcgactt ccgtcccagc
     1441 cgtgccaggt gctgcctcag attcaggtta tgccgctcaa ttcgctgcgt atatcgcttg
     1501 ctgattacgt gcagctttcc cttcaggcgg gattcataca gcggccagcc atccgtcatc
     1561 catatcacca cgtcaaaggg tgacagcagg ctcataagac gccccagcgt cgccatagtg
     1621 cgttcaccga atacgtgcgc aacaaccgtc ttccggagac tgtcatacgc gtaaaacagc
     1681 cagcgctggc gcgatttagc cccgacatag ccccactgtt cgtccatttc cgcgcagacg
     1741 atgacgtcac tgcccggctg tatgcgcgag gttaccgact gcggcctgag ttttttaagt
     1801 gacgtaaaat cgtgttgagg ccaacgccca taatgcgggc tgttgcccgg catccaacgc
     1861 cattcatggc catatcaatg attttctggt gcgtaccggg ttgagaagcg gtgtaagtga
     1921 actgcagttg ccatgtttta cggcagtgag agcagagata gcgctgatgt ccggcggtgc
     1981 ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga gggacagctg atagaaacag
     2041 aagccactgg agcacctcaa aaacaccatc atacactaaa tcagtaagtt ggcagcatca
     2101 cccataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa
     2161 acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat
     2221 tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg
     2281 aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     2341 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     2401 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     2461 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     2521 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     2581 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
     2641 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
     2701 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
     2761 cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
     2821 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
     2881 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
     2941 caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca
     3001 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
     3061 ttatatttta ctggatgaat tgttttagta cctagatgtg gcgcaacgat gccggcgaca
     3121 agcaggagcg caccgacttc ttccgcatca agtgttttgg ctctcaggcc gaggcccacg
     3181 gcaagtattt gggcaagggg tcgctggtat tcgtgcaggg caagattcgg aataccaagt
     3241 acgagaagga cggccagacg gtctacggga ccgacttcat tgccgataag gtggattatc
     3301 tggacaccaa ggcaccaggc gggtcaaatc aggaataagg gcacattgcc ccggcgtgag
     3361 tcggggcaat cccgcaagga gggtgaatga atcggacgtt tgaccggaag gcatacaggc
     3421 aagaactgat cgacgcgggg ttttccgccg aggatgccga aaccatcgca agccgcaccg
     3481 tcatgcgtgc gccccgcgaa accttccagt ccgtcggctc gatggtccag caagctacgg
     3541 ccaagatcga gcgcgacagc gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg
     3601 ccgtggagcg ttcgcgtcgt ctcgaacagg aggcggcagg tttggcgaag tcgatgacca
     3661 tcgacacgcg aggaactatg acgaccaaga agcgaaaaac cgccggcgag gacctggcaa
     3721 aacaggtcag cgaggccaag caggccgcgt tgctgaaaca cacgaagcag cagatcaagg
     3781 aaatgcagct ttccttgttc gatattgcgc cgtggccgga cacgatgcga gcgatgccaa
     3841 acgacacggc ccgctctgcc ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc
     3901 tgcaaaacaa ggtcattttc cacgtcaaca aggacgtgaa gatcacctac accggcgtcg
     3961 agctgcgggc cgacgatgac gaactggtgt ggcagcaggt gttggagtac gcgaagcgca
     4021 cccctatcgg cgagccgatc accttcacgt tctacgagct ttgccaggac ctgggctggt
     4081 cgatcaatgg ccggtattac acgaaggccg aggaatgcct gtcgcgccta caggcgacgg
     4141 cgatgggctt cacgtccgac cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct
     4201 tccgcgtcct ggaccgtggc aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa
     4261 tcgtcgtgct gtttgctggc gaccactaca cgaaattcat atgggagaag taccgcaagc
     4321 tgtcgccgac ggcccgacgg atgttcgact atttcagctc gcaccgggag ccgtacccgc
     4381 tcaagctgga aaccttccgc ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc
     4441 gcgagcaggt cggcgaagcc tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct
     4501 gggtcaatga tgacctggtg cattgcaaac gctagggcct tgtggggtca gttccggctg
     4561 ggggttcagc agccagcgct ttactggcat ttcaggaaca agcgggcact gctcgacgca
     4621 cttgcttcgc tcagtatcgc tcgggacgca cggcgcgctc tacgaactgc cgataaacag
     4681 aggattaaaa ttgacaattg tgattaaggc tcagattcga cggcttggag cggccgacgt
     4741 gcaggatttc cgcgagatcc gattgtcggc cctgaagaaa gctccagaga tgttcgggtc
     4801 cgtttacgag cacgaggaga aaaagcccat ggaggcgttc gctgaacggt tgcgagatgc
     4861 cgtggcattc ggcgcctaca tcgacggcga gatcattggg ctgtcggtct tcaaacagga
     4921 ggacggcccc aaggacgctc acaaggcgca tctgtccggc gttttcgtgg agcccgaaca
     4981 gcgaggccga ggggtcgccg gtatgctgct gcgggcgttg ccggcgggtt tattgctcgt
     5041 gatgatcgtc cgacagattc caacgggaat ctggtggatg cgcatcttca tcctcggcgc
     5101 acttaatatt tcgctattct ggagcttgtt gtttatttcg gtctaccgcc tgccgggcgg
     5161 ggtcgcggcg acggtaggcg ctgtgcagcc gctgatggtc gtgttcatct ctgccgctct
     5221 gctaggtagc ccgatacgat tgatggcggt cctgggggct atttgcggaa ctgcgggcgt
     5281 ggcgctgttg gtgttgacac caaacgcagc gctagatcct gtcggcgtcg cagcgggcct
     5341 ggcgggggcg gtttccatgg cgttcggaac cgtgctgacc cgcaagtggc aacctcccgt
     5401 gcctctgctc acctttaccg cctggcaact ggcggccgga ggacttctgc tcgttccagt
     5461 agctttagtg tttgatccgc caatcccgat gcctacagga accaatgttc tcggcctggc
     5521 gtggctcggc ctgatcggag cgggtttaac ctacttcctt tggttccggg ggatctcgcg
     5581 actcgaacct acagttgttt ccttactggg ctttctcagc cccagatctg gggtcgatca
     5641 gccggggatg catcaggccg acagtcggaa cttcgggtcc ccgacctgta ccattcggtg
     5701 agcaatggat aggggagttg atatcgtcaa cgttcacttc taaagaaata gcgccactca
     5761 gcttcctcag cggctttatc cagcgatttc ctattatgtc ggcatagttc tcaagatcga
     5821 cagcctgtca cggttaagcg agaaatgaat aagaaggctg ataattcgga tctctgcgag
     5881 ggagatgata tttgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
     5941 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
     6001 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
     6061 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
     6121 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
     6181 acacattgcg gacgttttta atgtactggg gtggtttttc ttttcaccag tgagacgggc
     6241 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     6301 gtttgcccca gcaggcgaaa atcctgtttg atggtggttc cgaaatcggc aaaatccctt
     6361 ataaatcaaa agaatagccc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     6421 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     6481 gcccactacg tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     6541 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     6601 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc cattcaggct gcgcaactgt
     6661 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     6721 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     6781 acggccagtg aattcctcga agcttgcatg ccggtcgact ctagaggatc cgggtgacag
     6841 ccctccgacg ggtgacagcc ctccgacggg tgacagccct ccgaattcta gaggatccgg
     6901 gtgacagccc tccgacgggt gacagccctc cgacgggtga cagccctccg aattcgagct
     6961 cggtacccgg ggatctgtcg acctcgatcg agatcttcgc aagacccttc ctctatataa
     7021 ggaagttcat ttcatttgga gaggacacgc tgaagctagt cgactctagc ctcgagacaa
     7081 gtttgtacaa aaaagcaggc tccaccatgg aaggcaaaat ttgtgcacaa atggacggct
     7141 acgagttcca gcgtgctgag cttgatgcat tggagagagt tgttagagat acctctgccg
     7201 agccaatgtc cttgacactg ccccttttgc gtcatatcac aaatgatttc tcagatgaat
     7261 ccaggatcgg acgtggagga ttcgctgttg tctatcttgg tgttttgcca tccggcctga
     7321 gaatagctgt taagaggttg tcaaatatag cttatatgaa tgaaagtgca ttccagaacg
     7381 aagtattcat cacaatgaaa gccacacata aaaatacagt gagattcatg ggatactgct
     7441 cacagattca aggcaaacta atagaacatg atggacaaca tgtctttgca cagcttgaag
     7501 agaggctaat ttgtgtggaa tacgcaccta agggaacatt agacgctcac atcggagatt
     7561 atggtgaact ggactggaac caaagatatc agattctgaa aggcatatgt cagggtttac
     7621 atcatctaca tgacgaaatg catgtctttc atggagatat caaacccgcc aacattctta
     7681 tcggcgataa cctagttcct aaaatctatg atttcggatt gagtcaaatg ttcgaggagg
     7741 aggaaacgga aagaattgtg gaaaacattg ccggtacatt cggctacatg gcacctgagt
     7801 tctgtacaaa caacatggta tccttcaagg ccgagattta cagtttaggc gttgtaatag
     7861 gagaacttct tatcggtaaa aaaggatggt ttgacgagga cgtgcggaag ctttttgttc
     7921 agcagttgaa aggccttcgc aaaactttgg tgaaggaagg cgcgttttcc agctgggaga
     7981 ataaatacca tcaagtgcgc acgtgtatgg agattggtca ggattgcatc gatccaaatc
     8041 ctcataaacg gcctacgctt ctcgaaatta ttcaacgcct taacgaagcc gaagacatga
     8101 attatagtgc ggcatcactc tggcaggttc agtctggtga tgaagaatca gatctctccg
     8161 atactgaagc gttggagaca gagaccacga gcgagtttct tccgtccgat gaagaaccgg
     8221 cgtccgtcgg taagaccggc gagacctcta cacaggaacc ggacaaaccc gatcttatat
     8281 ctaaacttcc cgcttcggtt gacctttctg atctcaaggt gcttgaaaag ataaccgatg
     8341 attttagcca tgagagaata gtcgggaaag acgggacttt taaaggatgc cataaggctt
     8401 tcgtttataa aggtgacatt cctctgaggg agatgattgc agttaaacgg ctcattggtg
     8461 ttgagatacc cttcgagaag ttcaagagag aggctgaaca attcatcagt ttggatcata
     8521 agaacatagt taaggttgca agttattgcc acgaccagag ccggggacat agactcgtcc
     8581 agttcaaggg gaagcctctg ccccaacttt ttaatgggcc tgagcaactg ttgtgttacg
     8641 agtacatgca caatggttct cttagagatt acttgatggg ccaaggctcc cgggtcatag
     8701 attggcagat gcggtacaag ctgataaaag ggacctgcgc aggcttgcat tatcttcata
     8761 agggtcgcgc aggatgtccg atcgttcacc ttaatctgag tcccagcaac gtgcttttgg
     8821 atcataatta cattccgcgg ataactggct tcgacttttc caagcttata ggagaaaaaa
     8881 acactaaatc tgtggtcttg aagctcaatg gacccatcgc ttatctgccc cccgacttct
     8941 tccacagcaa aggtacagat ctcaagtacc tggcgacagt tgatatatac tctttgggcc
     9001 tgatgatact cgagattgca acacagcagg aaatcaaggg catacatggt gttctgataa
     9061 agtcaatcga ggaaaactgg cgggaagagt cccaaatcac acgcctctat acctctcttg
     9121 gggctgatga gctgaggcag gtgaagatgt gtattgatat aggacttgac tgtgtcaaga
     9181 gtaacccaga gaagcgcccc actgcaggag caattatgtt gtggcttgat aaagaatcta
     9241 agccagtccc cgtgtcacgg gccggagcgg gcgttttgcc acggccccca gttccgacca
     9301 acatcaacca tgcggggcgc atccagggta tcccaatcga gaaatcacaa gtccttattc
     9361 cttcattcgc ttgtaatgaa tatagctctg tcaaaattca aaatttgcac ttgctgctct
     9421 ttatgggtag cgactacaag gaccacgacg gtgactacaa ggaccacgac atcgactaca
     9481 aggacgacga cgacaagatc acccagcttt cttgtacaaa gtggtgctcg acgattataa
     9541 ggatcatgat ggagattata aggatcatga tattgattat aaggatgatg atgataagta
     9601 aactagtcga tccaggcctc ccagctttcg tccgtatcat cggtttcgac aacgttcgtc
     9661 aagttcaatg catcagtttc attgcccaca caccagaatc ctactaagtt tgagtattat
     9721 ggcattggaa aagctgtttt cttctatcat ttgttctgct tgtaatttac tgtgttcttt
     9781 cagtttttgt tttcggacat caaaatgcaa atggatggat aagagttaat aaatgatatg
     9841 gtccttttgt tcattctcaa attattatta tctgttgttt ttactttaat gggttgaatt
     9901 taagtaagaa aggaactaac agtgtgatat taaggtgcaa tgttagacat ataaaacagt
     9961 ctttcacctc tctttggtta tgtcttgaat tggtttgttt cttcacttat ctgtgtaatc
    10021 aagtttacta tgagtctatg atcaagtaat tatgcaatca agttaagtac agtataggct
    10081 ttttgtgtcg agggggtacc gagtcgaggg gggatcaatt cccgatctag taacatagat
    10141 gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat
    10201 taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat
    10261 tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca
    10321 agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatcgggg
    10381 aaattcgggg gatcagcttg tgatcatgtt acatcacctc caagatcccg gacgagtgct
    10441 ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc
    10501 gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc
    10561 gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag
    10621 ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg
    10681 atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa
    10741 gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac
    10801 cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag
    10861 gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac
    10921 ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat
    10981 cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc
    11041 gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg
    11101 ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg
    11161 gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat
    11221 cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc
    11281 gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga
    11341 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa
    11401 cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc ccccgggatc
    11461 ttgcaggtcg agatcctcta gcggatccgg tgcagattat ttggattgag agtgaatatg
    11521 agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga
    11581 aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga
    11641 cgtatgtgct tagctcatta aactccagaa acccgcggct gagtggctcc ttcaacgttg
    11701 cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac
    11761 gtgactccct taattctccg ctcatgatca gattgtcgtt tcccgccttc agtttcaagc
    11821 ttgtttgggg atctagtgtt ttactcctca tattaacttc ggtcattaga ggccacgatt
    11881 tgacacattt ttactcaaaa caaaatgttt gcatatctct tataatttca aattcaacac
    11941 acaacaaata agagaaaaaa caaataatat taatttgaga atgaacaaaa ggaccatatc
    12001 attcattaac tcttctccat ccatttccat ttcacagttc gatagcgaaa accgaataaa
    12061 aaacacagta aattacaagc acaacaaatg gtacaagaaa aacagttttc ccaatgccat
    12121 aatactcgaa caagctagct tactcagtta ggtcgagtca tttttgatga aacagaagct
    12181 ttttgatatt tccatttgaa tattttggta tctgattagt gatgatttca gctaacatct
    12241 ctgggaattc aatactcatg gtcttatcca aaaatgtctg gaagcagtag gtaaggagat
    12301 tctcaaccac ctcatgcatg gagtccagaa gctttgtcag ttggtaaaac cgttgccagt
    12361 tctgactgga gttcccttcc cttttgacga tggcttttcc tagctctttg atataagtca
    12421 ttcgaatctc atcaaataac tcttggctct tcagaccttc cttaggaact gaggagagaa
    12481 gcagtaaggt tttcatacag agatactctt cataggatac ctgcaatctt tgtaattcag
    12541 aggagacaaa cagcatgtgt ttacattggt catacatgca gggtagagac attctctgct
    12601 cattaataat cagatcagga gcaaagcaga gcaggtttcc acttgattgt ctgtatgatc
    12661 tccaacccag ggcaaatgcc atgagaaaca tccatgagta ctgtagcagg gtcatttggt
    12721 catccaggtg taagtttctg aagcctggta tcgcctttgc ccatttcact gctgcaatca
    12781 cttgacgccc acctaacatg ttgagtgtgg tcataattct ccatgctgaa tctggaacag
    12841 agctatcata tcctgcatac aacacctcgg gttcaatcac ctccagcagt gacaccaagg
    12901 taggggtgag ctgtggtaat gcagcaggaa ctattgtttt gttaggattt tccgaagtgt
    12961 cttgtgagac tcctgcagtg gcttgctgaa ttggatcccc accgtactcg tcaattccaa
    13021 gggcatcggt aaacatctgc tcaaactcga agtcggccat atccagagcg ccgtaggggg
    13081 cggagtcgtg gggggtaaat cccggacccg gggaatcccc gtcccccaac atgtccagat
    13141 cgaaatcgtc tagcgcgtcg gcatgcgcca tcgccacgtc ctcgccgtct aagtggagct
    13201 cgtcccccag gctgacatcg gtcggggggg cgctcgacct aggaaaaatc agtagaaata
    13261 gctgttccag tctttctagc cttgattcca cttctgtcag atgtgcccta gtcagcggag
    13321 accttttggt tttgggagag tagcgacact cccagttgtt cttcagacac ttggcgcact
    13381 tcggtttttc tttggagcac ttgagctttt taagtcggca aatatcgcat gcttgttcga
    13441 tagaagacag tagcttcatt ggatctgcca ttgtttaaac tagagtcccc cgtgttctct
    13501 ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg
    13561 cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg
    13621 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
    13681 cggcagaggc atcttcaacg atggcctttc ctttatcgca atgatggcat ttgtaggagc
    13741 caccttcctt ttccactatc ttcacaataa agtgacagat agctgggcaa tggaatccga
    13801 ggaggtttcc ggatattacc ctttgttgaa aagtctcaat tgccctttgg tcttctgaga
    13861 ctgtatcttt gatatttttg gagtagacaa gtgtgtcgtg ctccaccatg ttgacgaaga
    13921 ttttcttctt gtcattgagt cgtaagagac tctgtatgaa ctgttcgcca gtctttacgg
    13981 cgagttctgt taggtcctct atttgaatct ttgactccat ggcctttgat tcagtgggaa
    14041 ctaccttttt agagactcca atctctatta cttgccttgg tttgtgaagc aagccttgaa
    14101 tcgtccatac tggaatagta cttctgatct tgagaaatat atctttctct gtgttcttga
    14161 tgcagttagt cctgaatctt ttgactgcat ctttaacctt cttgggaagg tatttgattt
    14221 cctggagatt attgctcggg tagatcgtct tgatgagacc tgctgcgtaa gcctctctaa
    14281 ccatctgtgg gttagcattc tttctgaaat tgaaaaggct aatctgggga cctgcaggaa
    14341 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
    14401 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
    14461 atgccaacca cagggttccc cagatctggc gccggccagc gagacgagca agattggccg
    14521 ccgcccgaaa cgatccgaca gcgcgcccag cacaggtgcg caggcaaatt gcaccaacgc
    14581 atacagcgcc agcagaatgc catagtgggc ggtgacgtcg ttcgagtgaa ccagatcgcg
    14641 caggaggccc ggcagcaccg gcataatcag gccgatgccg acagcgtcga gcgcgacagt
    14701 gctcagaatt acgatcaggg gtatgttggg tttcacgtct ggcctccgga ccagcctccg
    14761 ctggtccgat tgaacgcgcg gattctttat cactgataag ttggtggaca tattatgttt
    14821 atcagtgata aagtgtcaag catgacaaag ttgcagccga atacagtgat ccgtgccgcc
    14881 ctggacctgt tgaacgaggt cggcgtagac ggtctgacga cacgcaaact ggcggaacgg
    14941 ttgggggttc agcagccggc gctttactgg cacttcagga acaagcgggc gctgctcgac
    15001 gcactggccg aagccatgct ggcggagaat catacgcatt cggtgccgag agccgacgac
    15061 gactggcgct catttctgat cgggaatgcc cgcagcttca ggcaggcgct gctcgcctac
    15121 cgcgatggcg cgcgcatcca tgccggcacg cgaccgggcg caccgcagat ggaaacggcc
    15181 gacgcgcagc ttcgcttcct ctgcgaggcg ggtttttcgg ccggggacgc cgtcaatgcg
    15241 ctgatgacaa tcagctactt cactgttggg gccgtgcttg aggagcaggc cggcgacagc
    15301 gatgccggcg agcgcggcgg caccgttgaa caggctccgc tctcgccgct gttgcgggcc
    15361 gcgatagacg ccttcgacga agccggtccg gacgcagcgt tcgagcaggg actcgcggtg
    15421 attgtcgatg gattggcgaa aaggaggctc gttgtcagga acgttgaagg accgagaaag
    15481 ggtgacgatt gatcaggacc gctgccggag cgcaacccac tcactacagc agagccatgt
    15541 agacaacatc ccctccccct ttccaccgcg tcagacgccc gtagcagccc gctacgggct
    15601 ttttcatgcc ctgccctagc gtccaagcct cacggccgcg ctcggcctct ctggcggcct
    15661 tctggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    15721 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    15781 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    15841 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    15901 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    15961 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    16021 cttcgggaag cgtggcgctt ttccgctgca taaccctgct tcggggtcat tatagcgatt
    16081 ttttcggtat atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta
    16141 gactttcctt ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc
    16201 gcgagcgggt gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat
    16261 cctgctctgc gaggctggcc ggctaccgcc ggcgtaacag atgagggcaa gcggatggct
    16321 gatgaaacca agccaaccag gaagggcagc ccacctatca aggtgtactg ccttccagac
    16381 gaacgaagag cgattgagga aaaggcggcg gcggccggca tgagcctgtc ggcctacctg
    16441 ctggccgtcg gccagggcta caaaatcacg ggcgtcgtgg actatgagca cgtccgcgag
    16501 ctggcccgca tcaatggcga cctgggccgc ctgggcggcc tgctgaaact ctggctcacc
    16561 gacgacccgc gcacggcgcg gttcggtgat gccacgatcc tcgccctgct ggcgaagatc
    16621 gaagagaagc aggacgagct tggcaaggtc atgatgggcg tggtccgccc gagggcagag
    16681 ccatgacttt tttagccgct aaaacggccg gggggtgcgc gtgattgcca agcacgtccc
    16741 catgcgctcc atcaagaaga gcgacttcgc ggagctggtg aagtacatca ccgacgagca
    16801 aggcaagacc ga
//
