LOCUS       sequence-472261-       15363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression in Nicotiana benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15363)
  AUTHORS   Sung YC, Li Y, Bernasconi Z, Baik S, Asuke S, Keller B, Fahima T, 
            Coaker G
  TITLE     Wheat tandem kinase RWT4 directly binds a fungal effector to 
            activate defense.
  JOURNAL   Nat Genet. 2025 Apr 14. doi: 10.1038/s41588-025-02162-w.
  PUBMED    40229601
REFERENCE   2  (bases 1 to 15363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2025 Apr 14. doi: 10.1038/s41588-025-02162-w."
COMMENT     Sequence Label: pTA7001-AvrPWT4-GFP
FEATURES             Location/Qualifiers
     source          1..15363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1044..1277)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        complement(1539..1884)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    2399..2423
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2888..3538
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(3726..3743)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3977..3996)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            4219..4328
                     /note="incP origin of transfer"
     CDS             4361..4732
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      4859..5570
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  6193..6960
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             7152..7946
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             8245..9393
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    10989..11013
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(11260..11281)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11472..11491
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11616..11638
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11630..11647
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11631..11647
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11857..11903
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    11935..11959
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(12193..12195,12196..12198,12199..12909)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(12238..12259)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(12499..12518)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12846..12867
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(12910..12934)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             12942..13007
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      complement(13533..13785)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(14900..15083)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(14936..15115)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 aaaaaacaaa taatattaat ttgagaatga acaaaaggac catatcattc attaactctt
       61 ctccatccat ttccatttca cagttcgata gcgaaaaccg aataaaaaac acagtaaatt
      121 acaagcacaa caaatggtac aagaaaaaca gttttcccaa tgccataata ctcgaacaag
      181 ctagcttact cagttaggtc gagtcatttt tgatgaaaca gaagcttttt gatatttcca
      241 tttgaatatt ttggtatctg attagtgatg atttcagcta acatctctgg gaattcaata
      301 ctcatggtct tatccaaaaa tgtctggaag cagtaggtaa ggagattctc aaccacctca
      361 tgcatggagt ccagaagctt tgtcagttgg taaaaccgtt gccagttctg actggagttc
      421 ccttcccttt tgacgatggc ttttcctagc tctttgatat aagtcattcg aatctcatca
      481 aataactctt ggctcttcag accttcctta ggaactgagg agagaagcag taaggttttc
      541 atacagagat actcttcata ggatacctgc aatctttgta attcagagga gacaaacagc
      601 atgtgtttac attggtcata catgcagggt agagacattc tctgctcatt aataatcaga
      661 tcaggagcaa agcagagcag gtttccactt gattgtctgt atgatctcca acccagggca
      721 aatgccatga gaaacatcca tgagtactgt agcagggtca tttggtcatc caggtgtaag
      781 tttctgaagc ctggtatcgc ctttgcccat ttcactgctg caatcacttg acgcccacct
      841 aacatgttga gtgtggtcat aattctccat gctgaatctg gaacagagct atcatatcct
      901 gcatacaaca cctcgggttc aatcacctcc agcagtgaca ccaaggtagg ggtgagctgt
      961 ggtaatgcag caggaactat tgttttgtta ggattttccg aagtgtcttg tgagactcct
     1021 gcagtggctt gctgaattgg atccccaccg tactcgtcaa ttccaagggc atcggtaaac
     1081 atctgctcaa actcgaagtc ggccatatcc agagcgccgt agggggcgga gtcgtggggg
     1141 gtaaatcccg gacccgggga atccccgtcc cccaacatgt ccagatcgaa atcgtctagc
     1201 gcgtcggcat gcgccatcgc cacgtcctcg ccgtctaagt ggagctcgtc ccccaggctg
     1261 acatcggtcg ggggggcgct cgacctagga aaaatcagta gaaatagctg ttccagtctt
     1321 tctagccttg attccacttc tgtcagatgt gccctagtca gcggagacct tttggttttg
     1381 ggagagtagc gacactccca gttgttcttc agacacttgg cgcacttcgg tttttctttg
     1441 gagcacttga gctttttaag tcggcaaata tcgcatgctt gttcgataga agacagtagc
     1501 ttcattggat ctgccattgt ttaaactaga gtcccccgtg ttctctccaa atgaaatgaa
     1561 cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg
     1621 tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
     1681 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
     1741 tcaacgatgg cctttccttt atcgcaatga tggcatttgt aggagccacc ttccttttcc
     1801 actatcttca caataaagtg acagatagct gggcaatgga atccgaggag gtttccggat
     1861 attacccttt gttgaaaagt ctcaattgcc ctttggtctt ctgagactgt atctttgata
     1921 tttttggagt agacaagtgt gtcgtgctcc accatgttga cgaagatttt cttcttgtca
     1981 ttgagtcgta agagactctg tatgaactgt tcgccagtct ttacggcgag ttctgttagg
     2041 tcctctattt gaatctttga ctccatggcc tttgattcag tgggaactac ctttttagag
     2101 actccaatct ctattacttg ccttggtttg tgaagcaagc cttgaatcgt ccatactgga
     2161 atagtacttc tgatcttgag aaatatatct ttctctgtgt tcttgatgca gttagtcctg
     2221 aatcttttga ctgcatcttt aaccttcttg ggaaggtatt tgatttcctg gagattattg
     2281 ctcgggtaga tcgtcttgat gagacctgct gcgtaagcct ctctaaccat ctgtgggtta
     2341 gcattctttc tgaaattgaa aaggctaatc tggggacctg caggaaacta tcagtgtttg
     2401 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat
     2461 ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg
     2521 gttccccaga tctggcgccg gccagcgaga cgagcaagat tggccgccgc ccgaaacgat
     2581 ccgacagcgc gcccagcaca ggtgcgcagg caaattgcac caacgcatac agcgccagca
     2641 gaatgccata gtgggcggtg acgtcgttcg agtgaaccag atcgcgcagg aggcccggca
     2701 gcaccggcat aatcaggccg atgccgacag cgtcgagcgc gacagtgctc agaattacga
     2761 tcaggggtat gttgggtttc acgtctggcc tccggaccag cctccgctgg tccgattgaa
     2821 cgcgcggatt ctttatcact gataagttgg tggacatatt atgtttatca gtgataaagt
     2881 gtcaagcatg acaaagttgc agccgaatac agtgatccgt gccgccctgg acctgttgaa
     2941 cgaggtcggc gtagacggtc tgacgacacg caaactggcg gaacggttgg gggttcagca
     3001 gccggcgctt tactggcact tcaggaacaa gcgggcgctg ctcgacgcac tggccgaagc
     3061 catgctggcg gagaatcata cgcattcggt gccgagagcc gacgacgact ggcgctcatt
     3121 tctgatcggg aatgcccgca gcttcaggca ggcgctgctc gcctaccgcg atggcgcgcg
     3181 catccatgcc ggcacgcgac cgggcgcacc gcagatggaa acggccgacg cgcagcttcg
     3241 cttcctctgc gaggcgggtt tttcggccgg ggacgccgtc aatgcgctga tgacaatcag
     3301 ctacttcact gttggggccg tgcttgagga gcaggccggc gacagcgatg ccggcgagcg
     3361 cggcggcacc gttgaacagg ctccgctctc gccgctgttg cgggccgcga tagacgcctt
     3421 cgacgaagcc ggtccggacg cagcgttcga gcagggactc gcggtgattg tcgatggatt
     3481 ggcgaaaagg aggctcgttg tcaggaacgt tgaaggaccg agaaagggtg acgattgatc
     3541 aggaccgctg ccggagcgca acccactcac tacagcagag ccatgtagac aacatcccct
     3601 ccccctttcc accgcgtcag acgcccgtag cagcccgcta cgggcttttt catgccctgc
     3661 cctagcgtcc aagcctcacg gccgcgctcg gcctctctgg cggccttctg gcgctcttcc
     3721 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3781 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3841 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3901 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3961 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4021 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4081 gcgcttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc
     4141 atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg
     4201 tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc
     4261 cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg
     4321 ctggccggct accgccggcg taacagatga gggcaagcgg atggctgatg aaaccaagcc
     4381 aaccaggaag ggcagcccac ctatcaaggt gtactgcctt ccagacgaac gaagagcgat
     4441 tgaggaaaag gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca
     4501 gggctacaaa atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa
     4561 tggcgacctg ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg acccgcgcac
     4621 ggcgcggttc ggtgatgcca cgatcctcgc cctgctggcg aagatcgaag agaagcagga
     4681 cgagcttggc aaggtcatga tgggcgtggt ccgcccgagg gcagagccat gactttttta
     4741 gccgctaaaa cggccggggg gtgcgcgtga ttgccaagca cgtccccatg cgctccatca
     4801 agaagagcga cttcgcggag ctggtgaagt acatcaccga cgagcaaggc aagaccgagc
     4861 gcctttccga cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc
     4921 cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt
     4981 tgtggatacc tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact
     5041 tgaggggccg actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg
     5101 gcgacgtgga gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc
     5161 ccacagatga tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc
     5221 gcgactactg acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga
     5281 tgaggggcgc acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc
     5341 aagggtttcc gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca
     5401 atatttataa accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg
     5461 aaggggggtg cccccccttc tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc
     5521 ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag cgctggcagt
     5581 ccttgccatt gccgggatcg gggcagtaac gggatgggcg atcagcccga gcgcgacgcc
     5641 cggaagcatt gacgtgccgc aggtgctggc atcgacattc agcgaccagg tgccgggcag
     5701 tgagggcggc ggcctgggtg gcggcctgcc cttcacttcg gccgtcgggg cattcacgga
     5761 cttcatggcg gggccggcaa tttttacctt gggcattctt ggcatagtgg tcgcgggtgc
     5821 cgtgctcgtg ttcgggggtg cgataaaccc agcgaaccat ttgaggtgat aggtaagatt
     5881 ataccgaggt atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat
     5941 ttaaaaagct accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat
     6001 attgacaata ctgataagat aatatatctt ttatatagaa gatatcgccg tatgtaagga
     6061 tttcaggggg caaggcatag gcagcgcgct tatcaatata tctatagaat gggcaaagca
     6121 taaaaacttg catggactaa tgcttgaaac ccaggacaat aaccttatag cttgtaaatt
     6181 ctatcataat tgggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc
     6241 gatgactttg tcatgcagct ccaccgattt tgagaacgac agcgacttcc gtcccagccg
     6301 tgccaggtgc tgcctcagat tcaggttatg ccgctcaatt cgctgcgtat atcgcttgct
     6361 gattacgtgc agctttccct tcaggcggga ttcatacagc ggccagccat ccgtcatcca
     6421 tatcaccacg tcaaagggtg acagcaggct cataagacgc cccagcgtcg ccatagtgcg
     6481 ttcaccgaat acgtgcgcaa caaccgtctt ccggagactg tcatacgcgt aaaacagcca
     6541 gcgctggcgc gatttagccc cgacatagcc ccactgttcg tccatttccg cgcagacgat
     6601 gacgtcactg cccggctgta tgcgcgaggt taccgactgc ggcctgagtt ttttaagtga
     6661 cgtaaaatcg tgttgaggcc aacgcccata atgcgggctg ttgcccggca tccaacgcca
     6721 ttcatggcca tatcaatgat tttctggtgc gtaccgggtt gagaagcggt gtaagtgaac
     6781 tgcagttgcc atgttttacg gcagtgagag cagagatagc gctgatgtcc ggcggtgctt
     6841 ttgccgttac gcaccacccc gtcagtagct gaacaggagg gacagctgat agaaacagaa
     6901 gccactggag cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc
     6961 cataattgtg gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac
     7021 aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg
     7081 gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa
     7141 ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat
     7201 accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag
     7261 aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg
     7321 tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc
     7381 tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc
     7441 tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg
     7501 agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag
     7561 acagccgctt agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg
     7621 aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga
     7681 cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct
     7741 ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca
     7801 agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt
     7861 atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt
     7921 atattttact ggatgaattg ttttagtacc tagatgtggc gcaacgatgc cggcgacaag
     7981 caggagcgca ccgacttctt ccgcatcaag tgttttggct ctcaggccga ggcccacggc
     8041 aagtatttgg gcaaggggtc gctggtattc gtgcagggca agattcggaa taccaagtac
     8101 gagaaggacg gccagacggt ctacgggacc gacttcattg ccgataaggt ggattatctg
     8161 gacaccaagg caccaggcgg gtcaaatcag gaataagggc acattgcccc ggcgtgagtc
     8221 ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg accggaaggc atacaggcaa
     8281 gaactgatcg acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc
     8341 atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga tggtccagca agctacggcc
     8401 aagatcgagc gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc atcggccgcc
     8461 gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt tggcgaagtc gatgaccatc
     8521 gacacgcgag gaactatgac gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa
     8581 caggtcagcg aggccaagca ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa
     8641 atgcagcttt ccttgttcga tattgcgccg tggccggaca cgatgcgagc gatgccaaac
     8701 gacacggccc gctctgccct gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg
     8761 caaaacaagg tcattttcca cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag
     8821 ctgcgggccg acgatgacga actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc
     8881 cctatcggcg agccgatcac cttcacgttc tacgagcttt gccaggacct gggctggtcg
     8941 atcaatggcc ggtattacac gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg
     9001 atgggcttca cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc
     9061 cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc
     9121 gtcgtgctgt ttgctggcga ccactacacg aaattcatat gggagaagta ccgcaagctg
     9181 tcgccgacgg cccgacggat gttcgactat ttcagctcgc accgggagcc gtacccgctc
     9241 aagctggaaa ccttccgcct catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc
     9301 gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga acacgcctgg
     9361 gtcaatgatg acctggtgca ttgcaaacgc tagggccttg tggggtcagt tccggctggg
     9421 ggttcagcag ccagcgcttt actggcattt caggaacaag cgggcactgc tcgacgcact
     9481 tgcttcgctc agtatcgctc gggacgcacg gcgcgctcta cgaactgccg ataaacagag
     9541 gattaaaatt gacaattgtg attaaggctc agattcgacg gcttggagcg gccgacgtgc
     9601 aggatttccg cgagatccga ttgtcggccc tgaagaaagc tccagagatg ttcgggtccg
     9661 tttacgagca cgaggagaaa aagcccatgg aggcgttcgc tgaacggttg cgagatgccg
     9721 tggcattcgg cgcctacatc gacggcgaga tcattgggct gtcggtcttc aaacaggagg
     9781 acggccccaa ggacgctcac aaggcgcatc tgtccggcgt tttcgtggag cccgaacagc
     9841 gaggccgagg ggtcgccggt atgctgctgc gggcgttgcc ggcgggttta ttgctcgtga
     9901 tgatcgtccg acagattcca acgggaatct ggtggatgcg catcttcatc ctcggcgcac
     9961 ttaatatttc gctattctgg agcttgttgt ttatttcggt ctaccgcctg ccgggcgggg
    10021 tcgcggcgac ggtaggcgct gtgcagccgc tgatggtcgt gttcatctct gccgctctgc
    10081 taggtagccc gatacgattg atggcggtcc tgggggctat ttgcggaact gcgggcgtgg
    10141 cgctgttggt gttgacacca aacgcagcgc tagatcctgt cggcgtcgca gcgggcctgg
    10201 cgggggcggt ttccatggcg ttcggaaccg tgctgacccg caagtggcaa cctcccgtgc
    10261 ctctgctcac ctttaccgcc tggcaactgg cggccggagg acttctgctc gttccagtag
    10321 ctttagtgtt tgatccgcca atcccgatgc ctacaggaac caatgttctc ggcctggcgt
    10381 ggctcggcct gatcggagcg ggtttaacct acttcctttg gttccggggg atctcgcgac
    10441 tcgaacctac agttgtttcc ttactgggct ttctcagccc cagatctggg gtcgatcagc
    10501 cggggatgca tcaggccgac agtcggaact tcgggtcccc gacctgtacc attcggtgag
    10561 caatggatag gggagttgat atcgtcaacg ttcacttcta aagaaatagc gccactcagc
    10621 ttcctcagcg gctttatcca gcgatttcct attatgtcgg catagttctc aagatcgaca
    10681 gcctgtcacg gttaagcgag aaatgaataa gaaggctgat aattcggatc tctgcgaggg
    10741 agatgatatt tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
    10801 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
    10861 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
    10921 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
    10981 ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac
    11041 acattgcgga cgtttttaat gtactggggt ggtttttctt ttcaccagtg agacgggcaa
    11101 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
    11161 ttgccccagc aggcgaaaat cctgtttgat ggtggttccg aaatcggcaa aatcccttat
    11221 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca
    11281 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
    11341 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta
    11401 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
    11461 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcca ttcaggctgc gcaactgttg
    11521 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    11581 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    11641 ggccagtgaa ttcctcgaag cttgcatgcc ggtcgactct agaggatccg ggtgacagcc
    11701 ctccgacggg tgacagccct ccgacgggtg acagccctcc gaattctaga ggatccgggt
    11761 gacagccctc cgacgggtga cagccctccg acgggtgaca gccctccgaa ttcgagctcg
    11821 gtacccgggg atctgtcgac ctcgatcgag atcttcgcaa gacccttcct ctatataagg
    11881 aagttcattt catttggaga ggacacgctg aagctagtcg actctagcct cgagacaagt
    11941 ttgtacaaaa aagcaggctc caccatgaaa cccccctccg attcgacgga tgcctcaaaa
    12001 atcccgactt acaaaattaa acatccaggg cctcgttaca ttataactga cgatggtcga
    12061 gttgttgagg tcgaacgcaa ggaatcagga cgctatcaat taatcgatgg ggttttagta
    12121 cgtgtggaat atgaaaagaa aaccgggggt tcggaagggt caaaaacatc caccaacacg
    12181 cgcggtccga aaatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
    12241 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
    12301 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
    12361 ccctggccca ccctcgtgac caccttcacc tacggcgtgc agtgcttcag ccgctacccc
    12421 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
    12481 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
    12541 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
    12601 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
    12661 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
    12721 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
    12781 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
    12841 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactcacgg catggacgag
    12901 ctgtacaaga cccagctttc ttgtacaaag tggtgctcga cgattataag gatcatgatg
    12961 gagattataa ggatcatgat attgattata aggatgatga tgataagtaa actagtcgat
    13021 ccaggcctcc cagctttcgt ccgtatcatc ggtttcgaca acgttcgtca agttcaatgc
    13081 atcagtttca ttgcccacac accagaatcc tactaagttt gagtattatg gcattggaaa
    13141 agctgttttc ttctatcatt tgttctgctt gtaatttact gtgttctttc agtttttgtt
    13201 ttcggacatc aaaatgcaaa tggatggata agagttaata aatgatatgg tccttttgtt
    13261 cattctcaaa ttattattat ctgttgtttt tactttaatg ggttgaattt aagtaagaaa
    13321 ggaactaaca gtgtgatatt aaggtgcaat gttagacata taaaacagtc tttcacctct
    13381 ctttggttat gtcttgaatt ggtttgtttc ttcacttatc tgtgtaatca agtttactat
    13441 gagtctatga tcaagtaatt atgcaatcaa gttaagtaca gtataggctt tttgtgtcga
    13501 gggggtaccg agtcgagggg ggatcaattc ccgatctagt aacatagatg acaccgcgcg
    13561 cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa
    13621 ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat
    13681 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac
    13741 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatcgggga aattcggggg
    13801 atcagcttgt gatcatgtta catcacctcc aagatcccgg acgagtgctg gggcgtcggt
    13861 ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg
    13921 cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct
    13981 gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac
    14041 caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct
    14101 cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg
    14161 cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc
    14221 ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt
    14281 cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga
    14341 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga
    14401 aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg
    14461 tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag
    14521 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc
    14581 aataggtcag gctctcgctg aattccccaa tgtcaagcac ttccggaatc gggagcgcgg
    14641 ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta
    14701 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct
    14761 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag
    14821 acgtcgcggt gagttcaggc tttttcatat ctcattgccc cccgggatct tgcaggtcga
    14881 gatcctctag cggatccggt gcagattatt tggattgaga gtgaatatga gactctaatt
    14941 ggataccgag gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta
    15001 gctgatagtg accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt
    15061 agctcattaa actccagaaa cccgcggctg agtggctcct tcaacgttgc ggttctgtca
    15121 gttccaaacg taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt
    15181 aattctccgc tcatgatcag attgtcgttt cccgccttca gtttcaagct tgtttgggga
    15241 tctagtgttt tactcctcat attaacttcg gtcattagag gccacgattt gacacatttt
    15301 tactcaaaac aaaatgtttg catatctctt ataatttcaa attcaacaca caacaaataa
    15361 gag
//
