LOCUS       sequence-472268-       10205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Study the role and mechanism of the 3' UTR in regulating DVL3-P2 
            gene function..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10205)
  TITLE     Study the role and mechanism of the 3' UTR in regulating gene 
            function.
REFERENCE   2  (bases 1 to 10205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DVL3-P2
FEATURES             Location/Qualifiers
     source          1..10205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(747..1538,1539..1607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1370..1389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1608..1712)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1868..1916
                     /note="synthetic polyadenylation signal"
     CDS             complement(1980..2771)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2091..2110)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2701..2720
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(2823..3755)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     regulatory      3752..3761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3820..3955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        3972..4163
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4120..4140
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4445..4464)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4499..4518
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(7552..9204)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      9201..9210
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9251..9750)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9299..9318)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     9692..9715
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    9807..10090
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgacttaa taataataat agctaccatt
     4681 tattgagcac ctactctact gtgtgccagg cactgtgcta ggcacttttc gtacaatatc
     4741 taatttaaat cctcacaaca accctgtgag gtaggtatta ttatcatcat tttacatacg
     4801 agggaactga ggctcagaga ggttaaataa ctctatcaca caaccaaaaa tagtagagct
     4861 ggaattcaaa cccaaaacca cctaactcca aatcccatgc tccaatgaat taaagtagag
     4921 aggggagagg gagggccaaa ttcaagacag agtgttggag tgaaatggat atgatatgct
     4981 gataagatat gggaagaagg aatgtaagct cataaatgtt tctagctggt gagcctatgt
     5041 agattgtgat gaagtaacca ggagaggaat tcaggaggaa catacttggt tgggcagatg
     5101 agttgagtct atacggaact gtccatagag atgtccagta ggtaatacca atacagatct
     5161 aaagtctggg ggaagttggg ggttggagct atgtatttgg gaatcaccag catatcagca
     5221 gcaattgagg acatcaggat gaatgactcc attttgggag taaatagaaa ggaaagagaa
     5281 gtgaatcaaa gatggaacga tggaagcact gatactgaca ggcatgagca atcgttgaga
     5341 aattctcaag agagtcggac aagacagagt cagagagaga agtacatgaa acagccctgt
     5401 agtggtggct agaagccatg gactgggttg ggaacaattc tagagctccc agaaggcagg
     5461 caagattgag tcacacttcc aacaaagccc tctgcacacc aaaaccccag gaagtattgt
     5521 ttttctttgt tagtttgctt ttgagacaga atctcactct gtcacccagg ctggagtgcg
     5581 atctcaactc actgcaacct ccgcttgctt cctggattca agcgattctt gtgcctcagc
     5641 ctcctgagta gctgagatta caggcaggtg ccaaaacacc catctaattt ttgtattttt
     5701 agtagagaca gggttttgct atgttggcca ggctggtctc gaactcccga cctcaagtga
     5761 tccacctgtc tcagcctccc aaagtgctgg gattacagac gtgagccacc gcacccgtcc
     5821 aaggaagtat tgttaattga aataaatgcc aaaatgttcc taccttggtt taccccaaaa
     5881 cctggagcta agaacttcat ctcacttttg acaccccagc ccccaaaata tggaagccca
     5941 ggagagccag gagaatttat agcagaggct taaagagaaa gttatgattt gtttaaagta
     6001 gagaataagg tgaaaaataa aacctggtac tctgtctgga agtcctggaa gtctccttgc
     6061 ccaacctcaa ctggcctgtg ggctcctgtg tccttgctct gggatgccat ggtgaatgtg
     6121 aaaacagggg aggttgtgtg tgggggtggg aatggcctgt cggttgcaag gcgagtcctt
     6181 tgctgagccc agcctgagac ccagcttatg ggctttatcc aggtgagaaa atgctgggga
     6241 catgtgtgcg aggtttaagg tctaccagga aggggagaag ggtgtcaggg gaccagcccc
     6301 acaacctagg agaggattag gagggtccag ccctttgggc agggaccagc cctgaggctt
     6361 aaatgaactt gcataggcac ctgtgtatgc atctctgtgc atgtatgcat acatgagtga
     6421 gagagaaaga taaagagagc taaagaggcc cccctccccc cgccgcaggc tgccacacgt
     6481 ggtgcgatcc gattctctct ggaaaggagg agagtctctc atgcatgggt gaagttgggc
     6541 cctccccaga agcctagtga ctcattatgt taacgtgtgg gtgtgggggc tggacatcct
     6601 tgggaagccc tgaggggctg ctgaagcagg gttcctggtc tcctcggagg ctgggcctcc
     6661 cctcgctggg ggctcacaga aagtcaaaca gcccgaaatt cttggctgct ccaggcccag
     6721 ggtaaattgg gggaggaaaa aagaaagaaa agaaatgttt caggcctgga ggtgagatga
     6781 gcctgctggt tagtagggtt aggggtctga aggaaccagc acgcagggca ggcatggggc
     6841 aggggtagag cagagatgct ccctggaagc caccagctgc tcaggcaact gagggggccc
     6901 tctgggcctc tgatctaggc acaggcaggg atggcatgga tggctattgg aggcgactac
     6961 agctttaggt tgcaggactg aggcagcagg cagggggatg gttagtaggg gcagatgaag
     7021 caaatatatt ttggtttcag taatgtgggg taaagtcaag aggtttgcac acgtgcctga
     7081 tggggtgggg ttaatagagg gatgatgtag ctgaataaat ggtgaaaggg agctaaaggg
     7141 gaaacgagag aatgagggag tgagcgtgcc accaaggtca ttgctggggt caactcccaa
     7201 aatgggggtt gcagagggga gtaaggggta gcaccaacaa gtctggtccc agagacaaaa
     7261 ggggtcctgg gaagtcctgc gcagggatta gtgggcagag gaaacgaact ggggccagag
     7321 gcaggttagg gggctgcaga gccaatcggt aggtgcaccc caccctcgca gcaccctgcg
     7381 agcagttagc gcacctggat ttagttcctt ttatttccct ttcaggtacc agacaaagta
     7441 aaaaagacgg acggatggag agaggaacgc agccggctgg ggtgggagcg gaatggagct
     7501 gggggagggg ccctgcactc gaggctagcg agctcgttta aacaactaga attacacggc
     7561 gatcttgccg cccttcttgg ccttaatgag aatctcgcgg atcttgcggg cgtccaactt
     7621 gccggtcagt cctttaggca cctcgtccac gaacacaaca ccaccgcgca gcttcttggc
     7681 ggttgtaacc tggctggcca catagtccac gatctccttc tcggtcatgg ttttaccgtg
     7741 ttccagcacg acgactgcgg cgggcagctc gccggcatcg tcgtcgggca ggccggcgac
     7801 cccggcgtcg aagatgttgg ggtgttgcag caggatgctc tccagttcgg ctggggctac
     7861 ctggtagccc ttgtatttga tcaggctctt cagccggtcc acgatgaaga agtgctcgtc
     7921 ctcgtcccag taggcgatgt cgccgctgtg cagccagccg tccttgtcga tgagagcgtt
     7981 tgtagcctcg gggttgttaa cgtagccgct catgatcatg gggccacgga cgcacagctc
     8041 gccgcgctgg ttcacaccca gtgtcttacc ggtgtccaag tccaccacct tagcctcgaa
     8101 gaagggcacc accttgccta ctgcgccagg cttgtcgtcc ccttcggggg tgatcagaat
     8161 ggcgctggtt gtttctgtca ggccgtagcc ctggcggatg cctggtaggt ggaagcgttt
     8221 ggccacggcc tcacctacct ccttgctgag cggcgccccg ccgctggcga tctcgtgcaa
     8281 gttgcttagg tcgtacttgt cgatgagagt gctcttagcg aagaagctaa atagtgtggg
     8341 caccagcagg gcagattgaa tcttatagtc ttgcaagctg cgcaagaata gctcctcctc
     8401 gaagcggtac atgagcacga cccgaaagcc gcagatcaag tagcccagcg tggtgaacat
     8461 gccgaagccg tggtgaaatg gcaccacgct gaggatagcg gtgtcgggga tgatctggtt
     8521 gccgaagatg gggtcgcggg catgactgaa tcggacacaa gcggtgcggt gcggtagggc
     8581 tacgcccttg ggcaatccgg tactgccact actgttcatg atcagggcga tggttttgtc
     8641 ccggtcgaag ctctcgggca cgaagtcgta ctcgttgaag ccgggtggca aatgggaagt
     8701 cacgaaggtg tacatgcttt ggaagccctg gtagtcggtc ttgctatcca tgatgatgat
     8761 cttttgtatg atcggtagct tcttttgcac gttgaggatc ttttgcagcc ctttcttgct
     8821 cacgaatacg acggtgggct ggctgatgcc catgctgttc agcagctcgc gctcgttgta
     8881 gatgtcgtta gctggggcca cagccacacc gatgaacagg gcacccaaca cgggcatgaa
     8941 gaactgcaag ctattctcgc tgcacaccac gatccgatgg tttgtattca gcccatagcg
     9001 cttcatagct tctgccagcc gaacgctcat ctcgaagtac tcggcgtagg taatgtccac
     9061 ctcgatatgt gcgtcggtaa aggcgatggt gccgggcacc agggcgtagc gcttcatggc
     9121 tttgtgcagc tgctcgccgg cggtcccgtc ttcgagtggg tagaatggcg ctgggccctt
     9181 cttaatgttt ttggcatctt ccatggtggc tttaccaaca gtaccggatt gccaagcttg
     9241 ggctgcaggt cgaaaggccc ggagatgagg aagaggagaa cagcgcggca gacgtgcgct
     9301 tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc ccgcccctga
     9361 gcccgcccct gagcccgccc ccggacccac cccttcccag cctctgagcc cagaaagcga
     9421 aggagcaaag ctgctattgg ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg
     9481 tgctgtccat ctgcacgaga ctagtgagac gtgctacttc catttgtcac gtcctgcacg
     9541 acgcgagctg cggggcgggg gggaacttcc tgactagggg aggagtagaa ggtggcgcga
     9601 aggggccacc aaagaacgga gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag
     9661 gccagaggcc acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg catgctccag
     9721 actgccttgg gaaaagcgcc tcccctaccc ggtagatctc gatcctctac gccggacgca
     9781 tcgtggccgg agtactgcgg tttatctcga tggctacgag ggcagacagt aagtggattt
     9841 accataatcc cttaattgta cgcaccgcta aaacgcgttc agcgcgatca cggcagcaga
     9901 caggtaaaaa tggcaacaaa ccacccgaaa aactgccgca atcgcgcctg ataaatttta
     9961 accgtatgaa tacctatgca accagagggt acaggccaca ttacccccac ttaatccact
    10021 gaagctgcca tttttcatgg tttcaccatc ccagcgaagg gccatccagc gtgcgttcct
    10081 gtatttccgg actataaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    10141 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10201 tcttc
//
