LOCUS       sequence-472279-       12869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Study the role and mechanism of the 3' UTR in regulating C6orf89-P3 
            gene function..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12869)
  TITLE     Studying the role and mechanism of the 3' UTR in regulating gene 
            function. Batch 3.
REFERENCE   2  (bases 1 to 12869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C6orf89-P3
FEATURES             Location/Qualifiers
     source          1..12869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(747..1538,1539..1607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1370..1389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1608..1712)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1868..1916
                     /note="synthetic polyadenylation signal"
     CDS             complement(1980..2771)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2091..2110)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2701..2720
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(2823..3755)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     regulatory      3752..3761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3820..3955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        3972..4163
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4120..4140
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4445..4464)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4499..4518
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     regulatory      5358..5367
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(10216..11868)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      11865..11874
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(11915..12414)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11963..11982)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     12356..12379
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    12471..12754
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactttc ttatcaacca acacttttaa
     4681 ttgcaaaaag taaaaacctt cattacaata aactttaatt tacatacatt cttcacatgg
     4741 tggaagtctt taccagtttg tagtatcctg cacttgaaca catactaaaa gctcaagcca
     4801 ttcagagccg ctgtggctgt gacagctgcc tgcctccctc tgctccctgg gaccatccag
     4861 ggccaccaca ccctgctccc ggaaggagag ctgcagctga ccctggcacc tgtccccgat
     4921 gatggcggga gcactgcgct gagtccctgt cttgcctgtg actcctcctt ccccgaccct
     4981 cggcattggt tactgaccct tccccagggt ggaggtgaag gaagggaaga ctgcagccaa
     5041 catttttctg agggtaaaac tgtggaaaga agtatggagg gaagcctgtc tggataagat
     5101 ggggctttct gggaatgtca tgtgtctgct ggctcctgtc acctccctgg aagggtccac
     5161 agtgctgggg ggttgcagtg tgaagaatgg gatgctactg tgagtaaggg tggctgcaca
     5221 ggtgagcccc ctgccccaag gagctgctgt ggggagcgat ggtgtcaatg aagcctggca
     5281 cagagaagat gtcccaactc cccagtttct gtctgaaact cagacctcgg ctgggttcct
     5341 ttctccacac agtgcttgcc gccatggctc attcttggat tgatggctgc tgcctgcctg
     5401 acagacacac taggcagagg cggaagggtc tctggtccag ccctgtcaaa tcctgacagt
     5461 tccaggcctc cacttagtta tgtaacattc cttccctcca ctagtctgaa aaagtctgga
     5521 catttcgcag tctccaggga gaggaatatg acagatgtat ttggcactaa tgcttaaatt
     5581 atgcagggca gggaaaccag agacactgag ggacctggac aacagacaag ctggctgagg
     5641 gtttctcttc aaggccagca tttagtcaca ctgcgtccac ggtgggtgga cttggatgtg
     5701 gtgcacgaga aggagagcac ctgtccgtct ccctgacatg cttctggaag aggctgctgg
     5761 ggatgtggag actgggggtg gggaggacag ggaaatgcct gctgctggtg acacaaacac
     5821 attcccggag gcttccgtgt ttcatttgct acagagctgt ctccacaagc gccttcactc
     5881 tcctagcttc ctgaccctgc catgcatcgg ttctgtacac aacatctgca ggtccaaata
     5941 cagagagaat ttcagcctaa agtacaactt taaagcatat ctctgacact cccttccttc
     6001 aacaaattgt agtgggtgat gggtcaccac tgaaaggtaa tgagtgtgga acttgtatac
     6061 tcaagagagc ttttccttca ttgtcaggag agctcaaaat cctaagaggc ccttctgttt
     6121 ataactgatt ctcgaaaggt agacaaaatt gcctctctct gtgcaaacag acatgcgcct
     6181 ttctctctct ttctcctata acctggcttt ctcctttatg gacaaggaga tgcaaacatt
     6241 tgctttgggg tttgaacatt cactacgaaa gtgactgggt aataagcaaa ctcattccag
     6301 gctgtattaa aatgactgag gccatcacct gcagctaaga aatgtgagtt tttgttttaa
     6361 agtaagtgta cactgagtcc tatgctgggt tttggtagaa gggaacagaa aaccaagaat
     6421 tgaaaaacaa gtgatgtaaa ctccaagggg acccacaact ggaattgagc agagtccctc
     6481 ctctgtcaca catctggaag aatgtggaga caggaaaatg gctggacaca gttgtgtttc
     6541 agctacattc actcttaaaa agagttttca aatggggagg ggaaaagtga aaggattttt
     6601 ataaaagtca tatggttaga ttctttcctc ctccacttct gtccagcaga gttttaaaaa
     6661 caaaccctgc ggagttcctg gcttgctgac ccccatccct gggttctccc accccaacgg
     6721 aggcacatct gagtcaacga catccacagc tgtcttcttc ggcaccatgc cgtgatgccg
     6781 caggaaaatg atgtgctatg gaaccagcta cctacctgcc agcggttaac tgctctaagc
     6841 ctccacttat ttacttgcaa aatggggatc taacacctac tttacaggat tgggtgaaga
     6901 ttaaaggaga tcatgtatat caggcacttt gtaaactgga atgtactaga caaatgctat
     6961 tattcctttc tctcagaaca tgagcccccc caatctgctc tcctctcctc ccctcccctc
     7021 ccttcccttt ccctcccctc ccctcttctt ttctctctct cttttttttt ttcttttctt
     7081 tcctttcctt tgacagagtt tcgctctgtc gcccaggctg gaatgaagtg gcacaatctt
     7141 ggctcactgc aatctccgcc tccccggttc aagcaatttt cctgcctcag cttcctgtgt
     7201 agctgggatt acaggcgccc accatcacgc ctgggtaatt tttgtatttt tagtagagat
     7261 ggggtttcac cctgttggcc aggctggtct tgaactcctg acctcaagtg atctgcctgc
     7321 ctcagcctcc caaagtgctg caattacagg tgtgagccac catgcccagc ttgaaccact
     7381 tttattttat tccttctctt ccctaaaaaa aattgaggat aagctctgcc ttttttcatc
     7441 aaccataata agtatggtat ccattcacta cttgactgct ggagagctca ggacatttgg
     7501 acagaatgct tcaaactggg gccaaagtag cctgtctcct cagagagctg acttctgggc
     7561 atacgcatct ctgtagtggg aggccacatg aaagtgaacc taactcatgt acagaggtca
     7621 tcgccatgtc acttcctagt aaatgctcaa taaacttgta aaccaccccc tctctttaga
     7681 gccctacagt tttgttctta ttgtggtggt ggaggtgggg agttggttag gtggtcttat
     7741 cgtcaccctg cagccccact ttctcatcct gacaaccctc tttcccacac cgggtcatac
     7801 agagggactt ttgatgcttc cagctagatg gcaggggcat cctgtggcat cctgctgtgc
     7861 agggccttga tcatcagaaa gggactggct ggcaacccct agccactcct gtcgctctgg
     7921 ccagtagtag attctgctta tcaaaattaa cttgagaagt tgaactgctt ctgtgtatgt
     7981 agaaaaccct ctttggagaa ttttccccta atacaagttt acattcaagt ctgaactggg
     8041 tagattttaa gtaaaatgag tagtatatcc ataaaagtct ccacttatct tctgctacag
     8101 tcattttttg ttgaagtaca aaatgggact taggggactt tcccacggga tgcctcagaa
     8161 taccaacaca tgcaagggtt tcttatggac atttaagatg tgaagggcaa gagtgaaatg
     8221 gggaaaggaa tacagagtaa ctcacagaaa caaaacagag atctcagtaa tcaaaatagt
     8281 tgctttgttc ctaagaaacc ctctcttttg ttaatgggtc tgatttttaa aaactctcag
     8341 aggcctctaa agcacttgtt tacacatagc ccttccccat gtacagccag ggtaaggaca
     8401 ctgcagaagg ctgtaaaagc ttgcacaaag ccggcctcca gatgatccct ctgacttaaa
     8461 acctcttaaa tcataactgc ttaacaacat gactttcata cgtacacaag ttttgttgtt
     8521 cctgtattca tccacacaag gggttgatta agaaaaaatt ccctcaggta cagttcatcg
     8581 gagtttcact gaagcccgtg gaggaggctc tgatgtttct atcctgagct ctgtgaactc
     8641 tgcttgtcct ttgcaagccc atagacaaac aggccattca agtgctcaat ggtccccact
     8701 gctgttcatg agacccacag agaaagaggg cctgagagat gagatgcagg ctcctccagg
     8761 aatgaagagg gcttcacaac catcttcact cgtcattgtc tgcctctgat ccataattct
     8821 gacatagaca cctactgcat cctttgattt ctgcaattca gaaccagagg ccaaagtgta
     8881 gtttgctttt tcttcatctt ggcaatggca tgaccagtgg ccagctatct agtggtgtca
     8941 ataaaagaat aataaagaga gaaaaaaata aaaggaacag gaaaagaaca tccttattta
     9001 caccatttga ttgctaccta ctaaaagcac ttaggagaaa acaatgcaca tagtgaatca
     9061 tccaatcaga aacacacagc tttatattat tacaaaaaag aaaaacacct caccttgtct
     9121 ttgtttcgca gcacagacag aaatcatttt aagacagagg atcacatagg cagttgtaca
     9181 cccagctgac aaacactttg ggtctggatt tcctccccca accacattac atcagctttg
     9241 acacatctgt aaaacatctt ctctgcagaa agtcagaccc taaagatcaa acccagggag
     9301 gcaccctctc cccaggccag ctactctaac tcataaccct ctgaagacac ttggtaaagg
     9361 caacacagct gacgagagca gtgctgcctc agcagtagtg ggctctggcc ccgccaaggc
     9421 agggaggact agggccgagc ttctgagaag ccatccagat aggcaaggga gaaggggagg
     9481 gggtgcagtg gggataaaag aacctgatga aatggaagaa agcattacct tgggagttac
     9541 cattttgaat tattatataa tcagactcta gcctggcatc acggagactc caagagccac
     9601 ggacaatggg aaagaagtgt acttcttgaa tgcaattcaa agttatctga acttttttca
     9661 ctgtttctag attccacttt gcttgggaaa ctacaagaac ttctttaatc agccatggcc
     9721 ttccaaaaag tagagtgatg tggcaggcaa gacagggaca agcaggccca cctggtaaag
     9781 gatggctctg acattagaac ctgttggctg ccctctccac tgccagggaa ggaagctggc
     9841 catgcaggtg aagaaaggca ccagccctgt ttcctgcctg aagggcagca tcaaggtcat
     9901 agtgtccaaa ggcccggaag aggtcagtct cagatgggtg ctggcagagt cctagccaag
     9961 tggccaggct gaatccttcc tgagccacct gcaggaagag tcaggagggc gaggctctgg
    10021 ccactgcctt gcagctcaca gctggttcac acatgcatgc aaagggcagg taactaagta
    10081 accaaccccc agaaagcagt ttcatcgttt ttgtttttat ttttcccctt tcaacctggt
    10141 tttcggctct ggaagctgtt cctgtgcaca gttctatttc actcgaggct agcgagctcg
    10201 tttaaacaac tagaattaca cggcgatctt gccgcccttc ttggccttaa tgagaatctc
    10261 gcggatcttg cgggcgtcca acttgccggt cagtccttta ggcacctcgt ccacgaacac
    10321 aacaccaccg cgcagcttct tggcggttgt aacctggctg gccacatagt ccacgatctc
    10381 cttctcggtc atggttttac cgtgttccag cacgacgact gcggcgggca gctcgccggc
    10441 atcgtcgtcg ggcaggccgg cgaccccggc gtcgaagatg ttggggtgtt gcagcaggat
    10501 gctctccagt tcggctgggg ctacctggta gcccttgtat ttgatcaggc tcttcagccg
    10561 gtccacgatg aagaagtgct cgtcctcgtc ccagtaggcg atgtcgccgc tgtgcagcca
    10621 gccgtccttg tcgatgagag cgtttgtagc ctcggggttg ttaacgtagc cgctcatgat
    10681 catggggcca cggacgcaca gctcgccgcg ctggttcaca cccagtgtct taccggtgtc
    10741 caagtccacc accttagcct cgaagaaggg caccaccttg cctactgcgc caggcttgtc
    10801 gtccccttcg ggggtgatca gaatggcgct ggttgtttct gtcaggccgt agccctggcg
    10861 gatgcctggt aggtggaagc gtttggccac ggcctcacct acctccttgc tgagcggcgc
    10921 cccgccgctg gcgatctcgt gcaagttgct taggtcgtac ttgtcgatga gagtgctctt
    10981 agcgaagaag ctaaatagtg tgggcaccag cagggcagat tgaatcttat agtcttgcaa
    11041 gctgcgcaag aatagctcct cctcgaagcg gtacatgagc acgacccgaa agccgcagat
    11101 caagtagccc agcgtggtga acatgccgaa gccgtggtga aatggcacca cgctgaggat
    11161 agcggtgtcg gggatgatct ggttgccgaa gatggggtcg cgggcatgac tgaatcggac
    11221 acaagcggtg cggtgcggta gggctacgcc cttgggcaat ccggtactgc cactactgtt
    11281 catgatcagg gcgatggttt tgtcccggtc gaagctctcg ggcacgaagt cgtactcgtt
    11341 gaagccgggt ggcaaatggg aagtcacgaa ggtgtacatg ctttggaagc cctggtagtc
    11401 ggtcttgcta tccatgatga tgatcttttg tatgatcggt agcttctttt gcacgttgag
    11461 gatcttttgc agccctttct tgctcacgaa tacgacggtg ggctggctga tgcccatgct
    11521 gttcagcagc tcgcgctcgt tgtagatgtc gttagctggg gccacagcca caccgatgaa
    11581 cagggcaccc aacacgggca tgaagaactg caagctattc tcgctgcaca ccacgatccg
    11641 atggtttgta ttcagcccat agcgcttcat agcttctgcc agccgaacgc tcatctcgaa
    11701 gtactcggcg taggtaatgt ccacctcgat atgtgcgtcg gtaaaggcga tggtgccggg
    11761 caccagggcg tagcgcttca tggctttgtg cagctgctcg ccggcggtcc cgtcttcgag
    11821 tgggtagaat ggcgctgggc ccttcttaat gtttttggca tcttccatgg tggctttacc
    11881 aacagtaccg gattgccaag cttgggctgc aggtcgaaag gcccggagat gaggaagagg
    11941 agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggcct ccggaggacc
    12001 ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc gcccccggac ccaccccttc
    12061 ccagcctctg agcccagaaa gcgaaggagc aaagctgcta ttggccgctg ccccaaaggc
    12121 ctacccgctt ccattgctca gcggtgctgt ccatctgcac gagactagtg agacgtgcta
    12181 cttccatttg tcacgtcctg cacgacgcga gctgcggggc gggggggaac ttcctgacta
    12241 ggggaggagt agaaggtggc gcgaaggggc caccaaagaa cggagccggt tggcgcctac
    12301 cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa gtgcccagcg
    12361 gggctgctaa agcgcatgct ccagactgcc ttgggaaaag cgcctcccct acccggtaga
    12421 tctcgatcct ctacgccgga cgcatcgtgg ccggagtact gcggtttatc tcgatggcta
    12481 cgagggcaga cagtaagtgg atttaccata atcccttaat tgtacgcacc gctaaaacgc
    12541 gttcagcgcg atcacggcag cagacaggta aaaatggcaa caaaccaccc gaaaaactgc
    12601 cgcaatcgcg cctgataaat tttaaccgta tgaataccta tgcaaccaga gggtacaggc
    12661 cacattaccc ccacttaatc cactgaagct gccatttttc atggtttcac catcccagcg
    12721 aagggccatc cagcgtgcgt tcctgtattt ccggactata aaaggatcta ggtgaagatc
    12781 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    12841 gaccccgtag aaaagatcaa aggatcttc
//
