LOCUS       sequence-47228-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3040-04
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..762)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
ORIGIN
        1 aaatcataaa tcataagaaa ttcgcttatt tagaagtggc gcgccttatt tgtacaattc
       61 atcaatacca tgggtaatac cagcagcagt aacaaattct aacaagacca tgtggtctct
      121 cttttcgttt ggatctttgg ataaggcaga ttgagtggat aagtaatggt tgtctggtaa
      181 caagactgga ccatcaccaa ttggagtatt ttgttgataa tggtcagcta attgaacaga
      241 accatcttca atgttgtgtc taattttgaa gttaactttg ataccattct tttgtttgtc
      301 agccatgatg taaacattgt gagagttata gttgtattcc aatttgtgac ctaaaatgtt
      361 accatcttct ttaaaatcaa taccttttaa ttcgattcta ttaactaagg tatcaccttc
      421 aaacttgact tcagctctgg tcttgtagtt accgtcatct ttgaaaaaaa tagttctttc
      481 ttgaacataa ccttctggca tggcagactt gaaaaagtca tgttgtttca tatgatctgg
      541 gtatctagaa aaacattgaa caccataagt taaagtagtg actaaggttg gccatggaac
      601 tggcaattta ccagtagtac aaataaattt taaggtcaat ttaccgtaag tagcatcacc
      661 ttcaccttca ccggagacag aaaatttgtg accattaaca tcaccatcta attcaaccaa
      721 aattgggaca acaccagtga ataattcttc acctttagac atgttaatta aaccagcacc
      781 gtcac
//
