LOCUS       sequence-472280-       13335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Study the role and mechanism of the IVNS1ABP-P3 3' UTR in regulating
            gene function..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13335)
  TITLE     Studying the role and mechanism of the 3' UTR in regulating gene 
            function. Batch 2.
REFERENCE   2  (bases 1 to 13335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IVNS1ABP-P3
FEATURES             Location/Qualifiers
     source          1..13335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(747..1538,1539..1607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1370..1389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1608..1712)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1868..1916
                     /note="synthetic polyadenylation signal"
     CDS             complement(1980..2771)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2091..2110)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2701..2720
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(2823..3755)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     regulatory      3752..3761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3820..3955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        3972..4163
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4120..4140
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4445..4464)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4499..4518
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(10682..12334)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      12331..12340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(12381..12880)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(12429..12448)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     12822..12845
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    12937..13220
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactcaa atttaagacc ctctcaaact
     4681 aacaggctta gtgatgtaat tatggttagt agaggtacac ttgtgaataa agagggtggg
     4741 tgggtataga tgttgctaac agcaacacaa agcttttgca tattgcatac tattaaacat
     4801 gctgtacata ctttttgggt ttatttggaa aggaatgcaa agatgaaggt ctgttttgtg
     4861 tacttttaag actttggtta ttttactttt tggaaaagaa taaaccaaga attgattggg
     4921 cacatcattt caagaagtcc cctctcctcc acatttgttt tgccaatttg cacattaaat
     4981 gactcttccc tcaaatgtgt actatggggt aaaaggggta gggtttaaag atgtagacag
     5041 ttgggttttt taagggccct ttttcaataa ctggaacact ctataacaaa ggatacttat
     5101 ttaaatagat gacattgact atttttgttt ttattaaaag gaagcttaca tgcctaccaa
     5161 tatttaatct tttatgattg cctttttata actttttata ttctcagcag agtgctttac
     5221 caattgaagt aaaatgtggc aggctggagt tattgaagca gagtggcagt cttcagtttg
     5281 cagagtaggg gtctgtcttt taaactctga gtgcaaactt cagagttctt gccttggctg
     5341 cagttttttt ccttcaagaa tgcagtacta acatttattt gagtggagtt actgaacagt
     5401 aacatagctg tgatttttgg tatttgaaac actggtttta aatattttga cttgttgagg
     5461 gtatgtttta tatagcaaga cattatatag cagtaaaaaa tggtgtttta tcttctatat
     5521 aattcctgtt tttattatta acaaaacagt cctaaatagc agccctcaat tgtgaaaaaa
     5581 tttactttaa actacattag gttgtgaatg caggttttat cagaactatg tttttgttca
     5641 gtttatctgt tcatatggat aaatattggt tgggatgact tggtgtctaa tgtgtagtgc
     5701 tacacaccta acttatgggg ccaaaatagc atgtcctaat gcttgctgct gatttaaaca
     5761 cattaaaggt actttgcagg aaatccttgc accatgggat taatatccaa ttgctgcttg
     5821 tacactcatt cattactaaa agttttgaga aatttttttt tccagtaatg agcttaagaa
     5881 atttgtggaa aataactcac ctggcatctt acatctgaaa taaggaatga tataaggttt
     5941 ttttttctca cagaagatga agcacacagg aacctaatgg gccaactggg atgaggtgac
     6001 tattctgaga tgactattca gtggctaact tgggttagga agaaaataat taggtatttt
     6061 ctccaaatgt tcactggtac tctgccactt tatttctctc atctgttaca cagagaacca
     6121 ccaggaaagc aaatcagttt ggttggtaac tctgtaattc ctaactatca ctggtttggt
     6181 tctggactaa aactacattg acagattgaa tttgcctaat atgatgactg tttttaatat
     6241 ggatctgtat gtgttctatt cagcacaagg aaataaaatt ttagttgagg attcagcact
     6301 aaaatccttg agatcacttc tcatttcata agtgacatgc ttaatgcaaa taatatatgg
     6361 gaatactttg ggtcaaagtt ttcctgcatt accttggatg ccaaagaggt agtttggtgg
     6421 ccaaaaagta tcttttttta agaagctgtt gtagctagtc tgcatgaaca tgagaagttt
     6481 ccataaagga aaaaaagcag tgctcagtgg ccatttgtta ctgttaatct gtatctaaaa
     6541 ggtacatact ttcttcagag agggagaaaa tggcatagcc tcagcacaat ttaaaaaaaa
     6601 aaaaaagctt tttaaccagt aggaattttt gaatagtttc aaaataatat tgataatacc
     6661 acacttctgg tcaatttaat aaaacactct aaatttccta aaaaggtaga aaagctataa
     6721 aatatctaaa ccaaatatta ttggttatac tcgggtggat cttttattat gaactttaaa
     6781 tgtagggttc tgatttttgg cttctagtgt gtcatgtact ttaccactgc tcaacatgtt
     6841 ttcaaattcc caatataata atcatatttt agggaaaatc caggatttga gtatgtgata
     6901 tatcattaga atccttgctt aattaacaaa ttaggtgtgt gccctattac ctctcctgac
     6961 ctcagaattc ttaaaattga agtaggcaaa ggaggagccc cagtaggaca tttctgtaac
     7021 tgcatgcaga gttgccttca gaaaagtgaa tatgattcat ggaaaattca gttgctgtcg
     7081 taatttacac gcagctgctt tttgttccaa aaaaaaaggt ctgaagagtc agcaagtgag
     7141 acataagata caaggttaat taactaggtg taaaggagaa agaaaaacac tcatatatga
     7201 atccttaaat ccttggtaag actgagctac aagtttcatc ctataaacag gttttattaa
     7261 gttaaatcag tgtctctagg ttaaaaagga gtaattgcta tgaattgcag atatccttca
     7321 taataaaact gccagtttct caagaatatt aatgacactt tgaacaagta gagtttataa
     7381 acaggaacat acaaactcat gtgaatgtca tgggttcttc aacctatgag catcaataat
     7441 cttggccaat aaaataatga actttattaa cagctccaca ccttactggg tggagagaac
     7501 aaaggtgaga agcaaaactc aatcattatg ctggatctgt gtggaatgcc tttaacagag
     7561 gcatatatct gaatagaggc cacacacttg ctcaggatga ccaaaggaag taacataatt
     7621 ttagccagaa aaaaaaggca cacagaaaga cctaacttgg agtaatgaaa ctactgagat
     7681 gaaatgtcca gcaaggtaat aagatgagat ctgttaaaac gatttttaaa tgggtaaaat
     7741 atttgaagac actttgcaaa agaagttgat gccataagaa cacaggaaaa tactatcata
     7801 aatcattaag aaaatgtaaa ttaaaaccaa aatggcatac acctattgaa actgaatata
     7861 ccaaattctg cttacgatgc agagcaactt gaactctcat acattgctgg aggaactgca
     7921 aaggtgcaca ggcagtttaa aaaacaatcc atccattttc taataatctg tgacaaagca
     7981 atcccattcg taggtattta cccaagtgaa ggaacatatt cacacaaacc tgtatgtgaa
     8041 tatagcaact ttattcataa tcaccaaaaa ctgaattcag tgaagtagcc ttccacctgg
     8101 tgaatgaata aatttgtaca tccatggcaa tgcaatactt cttgggaata aaataatgaa
     8161 ctactgataa tatggataag tctcaagcat catggtaaat aaaaagccag actcaaaagc
     8221 ctccattagc tgggcatagt ggcatgcact tgtagtccca gctactcagg aggctgaggt
     8281 gagaggattg cctgagccca ggagttcaag gctgtagtat gctataatgt ctgaacagcc
     8341 actgcacttg ggcaacatag caagaccctg tctcttaaaa aaacagtctc catttatata
     8401 gcattcagga ataggaaaaa ctacagggaa ggcaaacaca tcaatggcct cctgagctgg
     8461 gaatgggagt taattacaaa gggacatgag ggaattttta ggggtggtgt gacttcaata
     8521 tcttgactgc tgatgattga ttgattacat gtttgacaga actcacagaa ctctacactt
     8581 acgaaggaaa aaaagttttg tttttctagc ttaaccaaag tggtaccaaa cctttaaaat
     8641 atcaagctta gaagaagggc tagaacatgt ccatatttta cctcattaga aataaagaat
     8701 aggatgttga aaatggtttg tgctttaaga agactgaacc aagctagtag ctcggatttg
     8761 ggtgtccacc acctcacctt ccaggcttat aactggtaat tctgctcaag aatctgtcct
     8821 cagtgttttc aacatcgaga attaatttga gacaaactac atctagttca taaacaaagc
     8881 tagaattaaa aaggattcct gtcacaggcc aaaactagtc tacaaaccct cagggtggcc
     8941 aattcttgct tttaacaaca gccaaagata ttaaagaatt ccatgaatac agagatggat
     9001 ttttaaaaat acaagtatat ataatactaa aaccctcagg aaagccaaca tcttgaggta
     9061 ctctggctcc agaagaatcc atttctgtgg gccatattcc aagatctaag aggtgtatct
     9121 gtttgacttt actgagtggc ttttcacctg acagaccaga caaacttaaa agctttcaag
     9181 tcaaactttg gtaggcaaag gtaaagtatg tcttttgttt tagagtaggt gagcctgcag
     9241 gtcaatttgt caaaaatatt aactaaccac aactatgaga cactacacac agtgtagttc
     9301 ctgccttcat ggggctcagt tctgttcact ggagtgggtt ttatatatac ccaataaata
     9361 gaagtgtgac tggcctgtcc aatctcgaag tcagaaaagg gccatgggta gcatcacatc
     9421 cttagagact ggatgcagtc accagacttt gaggaaagag ggaaattcat cccaacctgt
     9481 catgctagct aagagagcaa aaagtcaaga atatgaacaa tgtgacctag gctctgaaag
     9541 ctgaccaaga caccatctaa ataaacctgg actgacaatg aaaggacttt cgttctccct
     9601 taaaataatg ttcaaaatag caaggaaata gagcttcaga tctgtcttgt gtcacctggt
     9661 ctttcatgtg acagtcaaag atccacaatg tgaaagtggg attaacccat ctgtgaagtt
     9721 ctagtctgtg cagtcacaat tgaccactgc aggttttaac aagaggaccc tgtggggaca
     9781 caacagactt caattcttta gccagctttt ccttggctca gaatatggag agagctggag
     9841 gccctccaaa ccagaggatg ttcgctggtc ttcccaggag agaaatgaac tatcttttgt
     9901 aaaagtaagt ttcaaaggcc aagtgtagtg gctcatgcct gtaatcccag cactttggga
     9961 ggccaaggca ggtggataac ttgaggtcag gagttcaaaa ccagcttggc aaacatggtg
    10021 agatcctgtg tctactaaaa atacaaaaaa aaattagcca ggcatactgg tacatgcctg
    10081 taatcccagc tacttgggaa gctgaggtat gagaactgct tgaatccaag aggcggaggt
    10141 tgcagtgagc cgagatcgag ccactacgct ccagcctggg tgacagagtg agactctgcc
    10201 tcaaaaaaaa aaaaaaaaag gaagttgcaa aacagtaaag gaactctcaa cccctgcatt
    10261 acatgattag gacaacaaat agaaaagtaa taaagtttac gatcataaaa aaaaaaaaaa
    10321 agcaacaaag aagggagagg gaaaaacaaa caaaaaaaag caagacctga catttcaaat
    10381 gatttcaaat ggaagaaata aaagctaaaa caaaaatttt aaaacaatgt ttttgaataa
    10441 aaaagactag aaagttaaaa aaggacatct tcacatctca ataatattga aaaagttttt
    10501 tatataagac tgaagtaggc ataagggtaa atatgtgata agactgaaaa gaaattttaa
    10561 ggaaaggatt aacctatatt aaagcgatta aaaaaaaaaa aaagaggagt taccagaaca
    10621 aggaaaaaca aaaacccaac aataacactc gaggctagcg agctcgttta aacaactaga
    10681 attacacggc gatcttgccg cccttcttgg ccttaatgag aatctcgcgg atcttgcggg
    10741 cgtccaactt gccggtcagt cctttaggca cctcgtccac gaacacaaca ccaccgcgca
    10801 gcttcttggc ggttgtaacc tggctggcca catagtccac gatctccttc tcggtcatgg
    10861 ttttaccgtg ttccagcacg acgactgcgg cgggcagctc gccggcatcg tcgtcgggca
    10921 ggccggcgac cccggcgtcg aagatgttgg ggtgttgcag caggatgctc tccagttcgg
    10981 ctggggctac ctggtagccc ttgtatttga tcaggctctt cagccggtcc acgatgaaga
    11041 agtgctcgtc ctcgtcccag taggcgatgt cgccgctgtg cagccagccg tccttgtcga
    11101 tgagagcgtt tgtagcctcg gggttgttaa cgtagccgct catgatcatg gggccacgga
    11161 cgcacagctc gccgcgctgg ttcacaccca gtgtcttacc ggtgtccaag tccaccacct
    11221 tagcctcgaa gaagggcacc accttgccta ctgcgccagg cttgtcgtcc ccttcggggg
    11281 tgatcagaat ggcgctggtt gtttctgtca ggccgtagcc ctggcggatg cctggtaggt
    11341 ggaagcgttt ggccacggcc tcacctacct ccttgctgag cggcgccccg ccgctggcga
    11401 tctcgtgcaa gttgcttagg tcgtacttgt cgatgagagt gctcttagcg aagaagctaa
    11461 atagtgtggg caccagcagg gcagattgaa tcttatagtc ttgcaagctg cgcaagaata
    11521 gctcctcctc gaagcggtac atgagcacga cccgaaagcc gcagatcaag tagcccagcg
    11581 tggtgaacat gccgaagccg tggtgaaatg gcaccacgct gaggatagcg gtgtcgggga
    11641 tgatctggtt gccgaagatg gggtcgcggg catgactgaa tcggacacaa gcggtgcggt
    11701 gcggtagggc tacgcccttg ggcaatccgg tactgccact actgttcatg atcagggcga
    11761 tggttttgtc ccggtcgaag ctctcgggca cgaagtcgta ctcgttgaag ccgggtggca
    11821 aatgggaagt cacgaaggtg tacatgcttt ggaagccctg gtagtcggtc ttgctatcca
    11881 tgatgatgat cttttgtatg atcggtagct tcttttgcac gttgaggatc ttttgcagcc
    11941 ctttcttgct cacgaatacg acggtgggct ggctgatgcc catgctgttc agcagctcgc
    12001 gctcgttgta gatgtcgtta gctggggcca cagccacacc gatgaacagg gcacccaaca
    12061 cgggcatgaa gaactgcaag ctattctcgc tgcacaccac gatccgatgg tttgtattca
    12121 gcccatagcg cttcatagct tctgccagcc gaacgctcat ctcgaagtac tcggcgtagg
    12181 taatgtccac ctcgatatgt gcgtcggtaa aggcgatggt gccgggcacc agggcgtagc
    12241 gcttcatggc tttgtgcagc tgctcgccgg cggtcccgtc ttcgagtggg tagaatggcg
    12301 ctgggccctt cttaatgttt ttggcatctt ccatggtggc tttaccaaca gtaccggatt
    12361 gccaagcttg ggctgcaggt cgaaaggccc ggagatgagg aagaggagaa cagcgcggca
    12421 gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc
    12481 ccgcccctga gcccgcccct gagcccgccc ccggacccac cccttcccag cctctgagcc
    12541 cagaaagcga aggagcaaag ctgctattgg ccgctgcccc aaaggcctac ccgcttccat
    12601 tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac gtgctacttc catttgtcac
    12661 gtcctgcacg acgcgagctg cggggcgggg gggaacttcc tgactagggg aggagtagaa
    12721 ggtggcgcga aggggccacc aaagaacgga gccggttggc gcctaccggt ggatgtggaa
    12781 tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg
    12841 catgctccag actgccttgg gaaaagcgcc tcccctaccc ggtagatctc gatcctctac
    12901 gccggacgca tcgtggccgg agtactgcgg tttatctcga tggctacgag ggcagacagt
    12961 aagtggattt accataatcc cttaattgta cgcaccgcta aaacgcgttc agcgcgatca
    13021 cggcagcaga caggtaaaaa tggcaacaaa ccacccgaaa aactgccgca atcgcgcctg
    13081 ataaatttta accgtatgaa tacctatgca accagagggt acaggccaca ttacccccac
    13141 ttaatccact gaagctgcca tttttcatgg tttcaccatc ccagcgaagg gccatccagc
    13201 gtgcgttcct gtatttccgg actataaaag gatctaggtg aagatccttt ttgataatct
    13261 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    13321 gatcaaagga tcttc
//
