LOCUS       sequence-472286-       10609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Study the role and mechanism of the QSOX1-P2 3' UTR in regulation 
            gene function..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10609)
  TITLE     Study the role and mechanism of the 3' UTR in regulating gene 
            function.
REFERENCE   2  (bases 1 to 10609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: QSOX1-P2
FEATURES             Location/Qualifiers
     source          1..10609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(747..1538,1539..1607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1370..1389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1608..1712)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1868..1916
                     /note="synthetic polyadenylation signal"
     CDS             complement(1980..2771)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2091..2110)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2701..2720
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(2823..3755)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     regulatory      3752..3761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3820..3955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        3972..4163
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4120..4140
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4445..4464)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4499..4518
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(7956..9608)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      9605..9614
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9655..10154)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9703..9722)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     10096..10119
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    10211..10494
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactcga catgagcagt gggctaccat
     4681 tattaagcac ttagtgtttg cccagcatgg tacaacaatc tggtaggtgg gtagtttatc
     4741 tccaatgaca catggggaca agaggataag ttatcttgcc caaagtcaca cagccagtga
     4801 gtgttggaag ctggattcaa acccagacgt cagattccag agtccattct cttcaaggct
     4861 ggacttgacc ccaccctgac ccagggccca gatttgaggg ctctctctga gcccacctga
     4921 ctttcctcca gggtcaaggc tggctgggtc ccctggagcc ctctcctcca cccccacacc
     4981 cagaggacag gctgcatcag gggtcttccc acccccaccc cataccaagg ctgctggaga
     5041 ggagcctggc ccaaggtggc gctgcccctc ttggacctcc aagaatgccg catggagcag
     5101 cccctcaggg ctgtagtccc tctacaccta cattgggccc ctccagcctg gacccccacc
     5161 cctcccactt cctggcttcc catccttcag gtctcgcaga agcctcctca acaccctccc
     5221 tgcttggtct ctatccttca gaaccctcat cacaacctgt ccttgcctct agaacggaag
     5281 ccccatagca gcagggacca cgtatctgtt ttagcttcag tttctagccc agtgcagcat
     5341 tgtgaaaatc cttagtaata tttttaaaaa gttttcgaat tctacttttc tttgctgact
     5401 tggcatgtgc tttctcctct gcccgtaacc cccgttcctc cttatttcct tgctaacacc
     5461 tactcgtctt tcaaagctcc atgccactgt ctccttgagg aaattgttcc tgtgccccca
     5521 gtaggtaaca caccagacag aggcgagatt ctctatcccg ccccagacaa ggagcctttg
     5581 ctttgcaggg ctccccttgg gaccctctct tgccaggaga gcctctagag aagccatcta
     5641 gaagggcatt caagactcag caagctccag ctcccagcct ggcagactgg gtgggggagg
     5701 gcccagctgg tgggcgaggc tggtttatcg atgttgccga gagccctggg gaaatcaagg
     5761 tgaaaggcag ccacgctgct gaggaggccc acccgtttat caaccagagg ggctggggcc
     5821 gggcgaaaag aggcttggct gccagaggca gctgtgaggt gggaggtcca caccactccg
     5881 tggacctgga ggctgggcta cacactgtgc agcccgggct gctgcgacag tgtttccaac
     5941 atggcgctaa ttctaggtaa ctaagtttgg ctttagaaat accgtcactg gagccacagg
     6001 gcttgctgac agaaatcccc agcacttggc attgggcaag gagcccttag ggggtgaagg
     6061 gcaggcttat tcagcggcct tggtgcagtg ggcacacagg gcaccggccc tgggagcctc
     6121 tgtcttcctc ttgcaggcca gggttgcctc ctgattgtgg gttcctgtct gtctctcagg
     6181 cccccagcca gggccagccc aagggagcag ggggtgcaga gcacaaccag gaggatggag
     6241 ccaatgggtg cctggagcag gggcacagag agggagtcca acccaggagc ccagcaaggc
     6301 gctgcagcta aagaggtcaa gggaaacatg cagggagact gccatccctc cccagcccca
     6361 gctgccccat agcaggagtg gccttggcat cccacctgct ccacatagca cccaggccat
     6421 ctgagcagag tgaagatgtc aaagagaaaa ccaaatcgga ttgaggctcc tgctccccct
     6481 taatgaggtt cattatcagc agctctgatc cttgtaaaac ccagcaggcc ggattagggg
     6541 caggctctct aatcctggct gactcctccc caccgagtgg ccaaccctgc agctctgaca
     6601 aacaagccct tgcttcctgg aggagctgga attaggcagg tgcagacagg cttctccctc
     6661 accagcccag ggcactgagg caggagctct cagggaggat tggggcaggg ggtggcaagg
     6721 ctgcacatcc agaggcctgt ctccgtgcat gaaaaggcca tggggtttgg gtgcagacag
     6781 ttctatgggc agtctgtggc caggagctgt gtgtgctgag ggacatcgag tctggctgca
     6841 ttccaggtga ggctggtctc tgtgcattct gtccagactg gacactaggt ctccaagtag
     6901 caagttaaag aacacaccaa tgcttgcctt aacaaaaata atttattcca cacacctccc
     6961 aaggcagggg gtagcgtggg aatcaagcat gtgtaaggca ctgcccccgc cagacccttc
     7021 taacttctgc acactggaag gtgaaacctg gagagagaag acactcccct ccctagcttc
     7081 tacctggcac cctccaaaga tgagcattca tcttggagac caaaataaaa aaggacaaaa
     7141 gaccaggctc agagggagca gagctcaatg gggggaagtg aaagcagcca tcttctcctg
     7201 cagctaagcc agggcaaggc actagagacc cacatccttc ccatgccacc aactcgtcag
     7261 gtcccaccaa gcaagccact caccctacag cccagcaacc atggcttgcc tcacctggga
     7321 gaccagcacg acttccagaa gcttccaaac cccaccctga agccaaaccc cacccaggag
     7381 gtctgaggag ctggaggctg gccaggcata gagggcccgg ggtggtcctt cctcccaggg
     7441 tgcaaaggct tcttcattgt ccggaaggga gcagggtggg cggagcccca gcagctagtg
     7501 aggagttcca ttccagccca cccagctggg ggaccctcct ctggcttcac ttgccccagt
     7561 tgagggccaa gaccaagcag gagagactga ggctccagtg agaatgaggc aggacttcaa
     7621 ataagctgaa aaacaggaat gtggtgctaa gaggccaggg actgagctgc cctatgccca
     7681 ctgcccgggg ctgccctccc tgcacagcag gggaacctgg accctgctcc tgtcttttgt
     7741 gtcaaaggag ctctctgtcc tagcaacccc aagaagcttc actggagcaa ctcgttttcc
     7801 tgaatttccc acacttctag gccagacaag gagcaagggg tgggagggga gggaatgggg
     7861 tcagggggct tgaggtgcct agagatggca gctccctctc ccgcctcctc cccaggtggt
     7921 actcgaggct agcgagctcg tttaaacaac tagaattaca cggcgatctt gccgcccttc
     7981 ttggccttaa tgagaatctc gcggatcttg cgggcgtcca acttgccggt cagtccttta
     8041 ggcacctcgt ccacgaacac aacaccaccg cgcagcttct tggcggttgt aacctggctg
     8101 gccacatagt ccacgatctc cttctcggtc atggttttac cgtgttccag cacgacgact
     8161 gcggcgggca gctcgccggc atcgtcgtcg ggcaggccgg cgaccccggc gtcgaagatg
     8221 ttggggtgtt gcagcaggat gctctccagt tcggctgggg ctacctggta gcccttgtat
     8281 ttgatcaggc tcttcagccg gtccacgatg aagaagtgct cgtcctcgtc ccagtaggcg
     8341 atgtcgccgc tgtgcagcca gccgtccttg tcgatgagag cgtttgtagc ctcggggttg
     8401 ttaacgtagc cgctcatgat catggggcca cggacgcaca gctcgccgcg ctggttcaca
     8461 cccagtgtct taccggtgtc caagtccacc accttagcct cgaagaaggg caccaccttg
     8521 cctactgcgc caggcttgtc gtccccttcg ggggtgatca gaatggcgct ggttgtttct
     8581 gtcaggccgt agccctggcg gatgcctggt aggtggaagc gtttggccac ggcctcacct
     8641 acctccttgc tgagcggcgc cccgccgctg gcgatctcgt gcaagttgct taggtcgtac
     8701 ttgtcgatga gagtgctctt agcgaagaag ctaaatagtg tgggcaccag cagggcagat
     8761 tgaatcttat agtcttgcaa gctgcgcaag aatagctcct cctcgaagcg gtacatgagc
     8821 acgacccgaa agccgcagat caagtagccc agcgtggtga acatgccgaa gccgtggtga
     8881 aatggcacca cgctgaggat agcggtgtcg gggatgatct ggttgccgaa gatggggtcg
     8941 cgggcatgac tgaatcggac acaagcggtg cggtgcggta gggctacgcc cttgggcaat
     9001 ccggtactgc cactactgtt catgatcagg gcgatggttt tgtcccggtc gaagctctcg
     9061 ggcacgaagt cgtactcgtt gaagccgggt ggcaaatggg aagtcacgaa ggtgtacatg
     9121 ctttggaagc cctggtagtc ggtcttgcta tccatgatga tgatcttttg tatgatcggt
     9181 agcttctttt gcacgttgag gatcttttgc agccctttct tgctcacgaa tacgacggtg
     9241 ggctggctga tgcccatgct gttcagcagc tcgcgctcgt tgtagatgtc gttagctggg
     9301 gccacagcca caccgatgaa cagggcaccc aacacgggca tgaagaactg caagctattc
     9361 tcgctgcaca ccacgatccg atggtttgta ttcagcccat agcgcttcat agcttctgcc
     9421 agccgaacgc tcatctcgaa gtactcggcg taggtaatgt ccacctcgat atgtgcgtcg
     9481 gtaaaggcga tggtgccggg caccagggcg tagcgcttca tggctttgtg cagctgctcg
     9541 ccggcggtcc cgtcttcgag tgggtagaat ggcgctgggc ccttcttaat gtttttggca
     9601 tcttccatgg tggctttacc aacagtaccg gattgccaag cttgggctgc aggtcgaaag
     9661 gcccggagat gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa
     9721 tgccgggcct ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc
     9781 gcccccggac ccaccccttc ccagcctctg agcccagaaa gcgaaggagc aaagctgcta
     9841 ttggccgctg ccccaaaggc ctacccgctt ccattgctca gcggtgctgt ccatctgcac
     9901 gagactagtg agacgtgcta cttccatttg tcacgtcctg cacgacgcga gctgcggggc
     9961 gggggggaac ttcctgacta ggggaggagt agaaggtggc gcgaaggggc caccaaagaa
    10021 cggagccggt tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt
    10081 gtagcgccaa gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag
    10141 cgcctcccct acccggtaga tctcgatcct ctacgccgga cgcatcgtgg ccggagtact
    10201 gcggtttatc tcgatggcta cgagggcaga cagtaagtgg atttaccata atcccttaat
    10261 tgtacgcacc gctaaaacgc gttcagcgcg atcacggcag cagacaggta aaaatggcaa
    10321 caaaccaccc gaaaaactgc cgcaatcgcg cctgataaat tttaaccgta tgaataccta
    10381 tgcaaccaga gggtacaggc cacattaccc ccacttaatc cactgaagct gccatttttc
    10441 atggtttcac catcccagcg aagggccatc cagcgtgcgt tcctgtattt ccggactata
    10501 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    10561 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
