LOCUS       sequence-47233-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3050-07
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(107..127)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     722..750
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaatcataaa tcataagaaa ttcgcttatt tagaagtggc gcgccctaca attcgtcgtg
       61 ttcgaatttg tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag
      121 aaggaccatg tggtctctct tttcgttggg atctttcgaa agggcagatt gataggacag
      181 gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg
      241 gtctgctagt tgaacacctc catcttcaat gttgtgtcta attttgaagt tagctttgat
      301 tccattcttt tgtttgtctg cagtgatgta tacattgtgt gagttatagt tgtattccaa
      361 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
      421 aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt
      481 gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga aaaagtcatg
      541 ccgtttcata tgatctgggt atctagcaaa gcacattaga ccataaccca aagtagtgac
      601 aagtgttggc catggaacag gtagttttcc agtagtgcaa atcaatttaa gggtaagttt
      661 tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc cattaacatc
      721 accatctaat tcaacaagaa ttgggacaac tccagt
//
