LOCUS       sequence-47234-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3060-01
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..761)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
ORIGIN
        1 aatcataaat cataagaaat tcgcttattt agaagtggcg cgccttattt gtacaattca
       61 tcaataccat gggtaatacc agcagcagta acaaattcta acaagaccat gtggtctctc
      121 ttttcgtttg gatctttgga taaggcagat tgataggata agtaatggtt gtctggtaac
      181 aagactggac catcaccaat tggagtattt tgttgataat ggtcagctaa ttgaacacca
      241 ccatcttcaa tgttgtgtct aattttgaag ttagctttga taccattctt ttgtttgtca
      301 gcagtgatgt aaacattgtg agagttatag ttgtattcca atttgtgacc taaaatgtta
      361 ccatcttctt taaaatcaat accttttaat tcgattctat taactaaggt atcaccttca
      421 aacttgactt cagctctggt cttgtagtta ccgtcatctt tgaaaaaaat agttctttct
      481 tgaacataac cttctggcat ggcagacttg aaaaagtcat gttgtttcat atgatctggg
      541 tatctagcaa aacattgcaa accataacct aaagtagtga ctaaggttgg ccatggaact
      601 ggcaatttac cagtagtaca aatcaatttt aaggtcaatt taccgtaagt agcatcacct
      661 tcaccttcac cggagacaga aaatttgtga ccattaacat caccatctaa ttcaaccaaa
      721 attgggacaa caccagtgaa taattcttca cctttagaca tgttaattaa accagcaccg
      781 tcaccgt
//
