LOCUS       sequence-472363-       20572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 (–)-Eldh1 in Gordonibacter 
            urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20572)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 20572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF4-Elen0511-(–)-Eldh1-Accs(ElA2)
FEATURES             Location/Qualifiers
     source          1..20572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     regulatory      4350..4359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      7202..7229
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      14355..14382
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(14814..15665)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(15652..16491)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(17002..17973)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            19212..19299
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      19641..20035
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(20198..20241)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            20337..20446
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagattcag
     1501 gctttttcgt ctcggcggtg gcgcatgatg atttcgcgtg attcatcaag tgcttcaatg
     1561 agcggcttga ggcgttcttc gttttcctgc ttgtagatgc agtagatgat gaaatgcgca
     1621 tcctcgtccg aaatggggat gcttacggtt tggtttacat cttcgagaaa tttgagttgc
     1681 cgaagattct tctgcaagat gagcacgccg ccgtcaggag aggtagtcgc atagcttgtc
     1741 acggcacgcc ctagtttggg acgttttttt ggctcgaatc cgtgttcgtg gcatatctgc
     1801 tcgatccgtc tccatcccga aacggagtat tcatcgatga actggataaa cgtctcgtca
     1861 gctagttcgt ctatgctgat ttccgagcga tcagcaagtg gatggtcgcg atcaacgacg
     1921 gcaacaaatg gcgtttgaac aagcgggcgg aaaaccagcc ctcgctcttc gagcgcatga
     1981 gcctcgtcgt acgcgaacac catatccacc tcgtctcgat ccaactgatc gaacagaggt
     2041 cgatcctgat ggtgctcaaa cagaatgggt acatggtgct ggtcattgag cagcacggtg
     2101 gacagcgaga tgatgctcga caccgtgttg tcgtagagta tcccgtctac ccggatgggc
     2161 cgttctttca cgaccgtggc tatggctgct tgggccctgt cgtatgcatc gaccatctcc
     2221 atcgcgcatc cgaacagaat acgtcctgct tcgctcagtt gcacggttct tcggtcgcgg
     2281 atgaataact cggcgtcgaa ctcgcgttcc aacgcaacga tatgcttgct cagcgcaggc
     2341 tgggtcatat gcagcttctc agctgcttcg gtgtagttga ggcagcgcgc caaaacgatg
     2401 aactcgcgat agaagtcgat gttcatagga tccccttccg cccgttagag cgttccccgc
     2461 ttcatggtat cacgtcgacg gcgtccgcgc gtttccggat tgtctgtggt agaaaacgga
     2521 ataacgatat gcaccttcat ctcggttggc ctgcgacgtt cgttttcatt atttccatat
     2581 gctttggctg ctcgccatac tcaggtttaa ggaaagcctt gagagaagga gggtgtatgg
     2641 ggaagacgac cgtgacaagg cgcgcgttcg ctcagcttgc cgccgcgaca ggcgcaatag
     2701 ccgctatggg tgttggaact cgtcctgcgg tggcgcttac cgatggcaat tcggggacgg
     2761 cgggcgagcg ggggatcaag aagatacgct cgtgctgccg cggctgcgga aaggtcgaat
     2821 gcggtgtgtg ggtgtacatc caagatggca aagtggttcg aaccgaaggt gacgaaacct
     2881 gcttcaacac tatgggcaat cattgcagca aggggcaagc gtccattcag gctgcatatc
     2941 atcctgatcg tatcaagttc ccaatgaagc gcacgaaccc gaagggcagc gaagatccag
     3001 gttgggtgag gatcagctgg gacgaggcct accggaccat tgcggacaat attatgcagt
     3061 tgcgtgagaa gtacggtcct gaaagcttgt tcacgtggtg cggtacgggc cgacagtggt
     3121 gcatgcagtc cgatgctggc atggcgcttg agctgttcgg tacgccgaac atcatcgcgg
     3181 cgtaccaagt gtgcaagggt ccgcgccatt tttcgtcgcg tctcgacaac gttcaggcgt
     3241 ggtcttggag tgaggtgata aaccattcga cgaaatacgt ccaatgggct accgatccct
     3301 ccgtttcgaa ttacgacgat tcgtctcgtt tggtaattga tgtggcgcgc gaagctgagg
     3361 cttttatcgt ggttgaccct cgcctgtcga atcttggccg cactgcgaag tattggttaa
     3421 acttgcgacc tggcaccgat aatgccatgg cgctaggatg gtgccatatc atattgaagc
     3481 acgatctggt ggattggcag ttcgtgaaac gctggtccaa tgcctcgttc atcgtcgtgc
     3541 ccgatatgga cccttcgggc tacaccgagg cggttcaaaa cacgaaaagc ccctacgaat
     3601 atcgtacgcg tttgctgacc gaagccgaca tagatccttc tatggttgac tgggagattg
     3661 aaggcgaggg gaatccaaaa cgttatcttg tgtacgatca gatcaatcgg cgctggacat
     3721 attggcaagc cgatcccgaa gacgcgcatt gggaaggcga gacctggaca aaacaaacct
     3781 ctggattcac gcaagacgtt tcgcgtcttc gcgacgacga atcgaaggtg gctggctgga
     3841 tcgcggatct gtcggaattc gatccgcgta tcgatccggc gctcaccggg gaattcgaag
     3901 taaggcttaa ggacggaagt acgcatatcg gccgtcctgc atgggatttg tgggctgagt
     3961 acctgcaaca attcactccc gaacgagtgt cggagatcac gggcgtggat gcccagctta
     4021 tcgaagacgc cgtggttgaa tgggcaacgc gcgatgaccc gcgcataccc aacggcggta
     4081 taaattacgg tctgggcgtg gagcatgcgg gcaactcaac gcagaattgc agggccatta
     4141 tggcggcttg cgcaatggta ggcgccatcg acacgccggg cggccagcgc ggtgcgacga
     4201 acggatggac cgaacagtcg ggtccgtgcg ccatgcttcc gtcgatggcg gcattcgcgt
     4261 tcatgcccac gcccgacttg tcgctcaaga tggccggaaa tgagaagatg ccgcttctat
     4321 actggtacgg tgtgtggtgc gatgccaatg ccgccatgga atgcgcgcac aaggagccgg
     4381 atgcgcccta tgagattcat ggtggcatga tcggctcggg cgaccatatg aacatgggca
     4441 acgcaacgta caattgggaa gcgctcaaca tgctcgactt tctgttcgag gcgaatctgt
     4501 ggcattcccc tacttcaggt gcggccgaca tcctccttcc ggtctgccat tggaccgaga
     4561 tcaacgcgta tcgcattgcc caaggggcat ccggtgggtt tggcctatgc gtgaaagctg
     4621 ttgatcctcc tggcgaatgc aaaagcgatc cgttgtactt catggagctg tcgaagtatt
     4681 tcggcgtccc agcgtttgac ggcgacgatc cgtggctcga gaacaaacct gatgccgatc
     4741 tcgaaatcga aaacctcacg attcagtgct gtgtccaagg atgcgcgcca tacaacaatt
     4801 ggaacgagtt ggaggctgca ttccaagagc acggttggtg gaacatgaaa cgcgagatcc
     4861 cggaagactg gggcacgtac cgtcgatacg aggtggggca ggcgtatcgg ttggctcccc
     4921 atcagcaacc ggctcagctg aacatcaata aaccggggtt ccccacacct acgatgaaac
     4981 atgagttttg gtgcacttcc atcgaatcgt tcttcccgga gggggcggat ggccctgagc
     5041 ttgcacccgg gttcacttcc gaagcgctgc cttattatgc agagcccgca cacggccctg
     5101 tggtcgatgc ggaaacgtac aaggagtatc ccattacctg catcactgga cgacgcatac
     5161 cggtgtactt ccactccgag caccgacagc tgccctggtg tcgcgagctt tggccggtgc
     5221 ctcgcatgga gatcaatccc gatacggctg ctgaacttgg actcgaacaa ggagattggg
     5281 cctggatcga gagcccttgg ggcaaggtgc gacagacggt cgatctctat tacggcatta
     5341 agccgaacat gatcaacgcc gagcatcaat ggtggtatcc ggagttggct caagcggaca
     5401 aaggatatga gttgtcatgc atcaattgca ttaccgatcg gaagactcag gataaataca
     5461 atggatcgtc gaatgtgcgc acctatccag tgaaggtgta caaagccacg cctgagaatt
     5521 ccccctttgg gaatcctatc ccttgcggaa acgatgggac tgagatcatt catgactctt
     5581 ctgatcctcg cctcaagcta tgggaaatcg gcgctgctgg catccatccg gatcacttcg
     5641 agtaggagag ggggaattat gacgcagtac gctatcgtca ccgatctcga tcgttgcgta
     5701 ggttgtcttg cctgttcggt tgcctgcaag gtagtcaacg gcgttccgac gggctcgttt
     5761 tggaacaaga cattgcgcat tggcccgaat cctcgagaag gcggcagtgg gcaatggccc
     5821 gatgtggagc tgtattttct caatgtgcag tgccagcact gcgagaaccc cgaatgcgtg
     5881 aaggtgtgcc cgacgggcgc ctcgcacaag accgaggacg gcaccgtgca gatcgacaag
     5941 tcgaagtgca tcggctgcca gttctgcgcg atgtcgtgcc cgtacggggt gcgctacctc
     6001 aacgaggagg agcgcgtcgt ggagaagtgc accttgtgcg agcagaagat cgctcagggc
     6061 gaacttcctc agtgcgtgag tcaatgctgc ggtatggcac gctggttcgg cgatgcggac
     6121 gaaggtatcg aaggtttcgt cgggcctcgc ggcgagatac tgggcgagta tatatcgcca
     6181 ttctcggatg atcaggtgta tagcctgccg gatgtgggca ataagccgtc gctcaggtac
     6241 atcttgcgcg atatgaaatg gcaaggaggc ggtgaatgat ggaactgcaa tggcctttga
     6301 ttttgttcac cacgctggtt gcgtggagcg cggggctgtt cgggacgcag gcgctgatgg
     6361 cggtgttcgg cgtcgggaag aaggcgcagg tgccggcttg ggtttgctcg gccgtcttgc
     6421 tggcggccgg cgggatcgcg gtgttcttcc atctcgagca ctgggagcgg atcttcaacg
     6481 ggttcgggca tttgacgagc gggatcacgc aggaattgat cgcgatcgtg gtcttggccg
     6541 tggtggctat cgtctacttg gtgctcatgc gcaagtccga cgacggcgcc agcgtgccga
     6601 agtggctggc atgggtctcc gtcgccttgt cggtcgtgct cgtggccgtc atggcgcaca
     6661 gctacacgat ggccgcccgc cccgcctggg actcggccct ctggatcctc tacgtcctcg
     6721 gcaacgcctg cgtgttgggc cccgcgacgt tcgccctcct ctccgccttg gctgcagggg
     6781 gaccccggga ccagcccgcc gagcgcgcag ccgacgccgg ggcccccgcg ggtctcgcgg
     6841 ccctcgccgg cgccgccctc aacgccctcg cggccctcgc cttcgccgcc ttcctccagc
     6901 tctccgccgg ctccttcgcc gacgtcggcc tctacttcga cccgacgcac cccacgaagg
     6961 ccatggccga cgccgccgcg acggtcgcct cccaggcccc gctgctgtgg ctgggcgccg
     7021 tggccgtggg cgcgatcgtg cccctggccg ccgccttcct aggcaggcgc acggggaact
     7081 ggaagctctg ggtcccggtc gcgatagccg ccgccctggt cggcgccgtc tgcatgcgcg
     7141 tcgtcttcta caacctgggg ctcagcgtgt tcatgttcta ctagactagt cgactcacac
     7201 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc
     7261 agattattaa tccggctttt ttattattta attcctgtac cgctggttgc cacgacgccc
     7321 tcgaagtctc cttcgagggc gttctgtcgt ttgcaagaag ggtctggaga ggcaaatcgc
     7381 gctcgaaggg gcttcgaggc cggggtaggt cgggagggtg gaatccggat gtttcacgtg
     7441 aaacatccgt tcttttacgg ccggtgtttc gcgtgaaaca ttttgcggaa aggactcctt
     7501 cccttcccgt gcgccacccc aacgccccgc tcctagcgct cgtacacctg gtcgcagttg
     7561 agcagatcct tcatggagtg ccagatctgg tgcgggttgc ccggcgggtt cttgagcggc
     7621 ttcatcggat agcgctcctg cgcgcccgac agcagatcga cggcgaacac ttcgtcggac
     7681 agctcgagcg cgccttccgg gcagatgtcg gtgcagcagc ggcacttcac gcaatcgacc
     7741 ggacgatgcg tcacgccgat cgagccgtcc tcacccgcgt acttcgcgat ggccccggtg
     7801 gggcagaacg tggcgcacat ctggcacgac gagcacttct cgggatcgat gatcacatgg
     7861 ccccacaagc gcgtgtccat gagcacatcc tgcggctgcc ccaacgaatc gagcgcggcc
     7921 agcaaacgtt cgcggcggtc gggaatgaag tgaggcagcg tgccgtcctt gcccaccttc
     7981 acgaacctcg gctcccgccg ctcctccaca cccaatgcgt ccttgacggc gtagtcagcc
     8041 gtcaccgccg caacgttctt agcctcgtcc ttcatgctgg agaagaagct ccgacggctc
     8101 gcgtcgtagc cctcgtcgcc cgcccggcgc acggatgagg gaagcttttc cgccaggtcg
     8161 atgcgcatat cgttgttcca ggttgcaagc agcgcgcttg ccgtgtcgca cacaacccgc
     8221 gccatctcga aacccgagac atgctcgcat tgcgcgcagc gcgcctgcac gaggctcacg
     8281 cggcgcacgc ccaacgccgc cagcgtgacc agcaggctct cctccacgcg ccccaggcac
     8341 gtgacgccca ccaccttctc ggggtcgtac aacccgtcgg ccgcggcaag cagctgatcg
     8401 caggcgataa ccacctgtcc gtcggccacg tcagccgccg cgcggcatgc ttgcagaagc
     8461 tcggcatcgt tcggacgatg cgcttcaagc gcgcaggtgg gacacacggt ggcgcaggtt
     8521 ccgcacccga tgcaccgctc gggttcgatg accagctcgt tgtcgtcata ggagatgcaa
     8581 ccgctcgtgc acacgtcggc gcacttcatg cacgttgcgt tgcgattgcg cacaatagcg
     8641 caacgaccct gatgaaccgt aatgtcggcg ctctgaagtt gttcgagcag accgaggaaa
     8701 ttcgtcacgt gagccatgac ttcacccttt ctcgtctacc cccttgcacc gcacagtata
     8761 accactttcg ccgtctcacg cgagaaaaaa cgcgcttgac accttcgaca ggcctcccta
     8821 tcatgtggcc ttggggagtc cgccggcacg ctgatcgcgt gcgaagcgga cattgtttgc
     8881 aaaagtggga acctcagggg tgaggtttcg cggtgctgtg cgccgtaagt gcaaacaatg
     8941 tctgacgagg ggcggaacat gactcacgat tccacgaacc agcatctttc tcttgagccc
     9001 gactacgacg ggttcgatcc acttgaagat ttcgaagacg atgcgaccga ggtcgaaggc
     9061 cgtgccgacg agttcgtcgc cacggtgagc gccgccgaag acgcgctcgc tcacgacgcg
     9121 atggcacccg ccacgcccgc catctgcgaa aagcccgctg aagagcgcat cgccgacttg
     9181 ttggaggcca tggcgccgcg ccgcacggtg ctgctgggca tcctggccgc ctgcgccgag
     9241 ccgcagccgg catccgccgt gaacgctcgc gtcgacgagc tgcaggagaa caacttctcg
     9301 gtgtacagcg cggccaacct gtgctccttg ctggagaagg cgggcgccat cgagcgcgtc
     9361 acggccgagg gcgaacccgc ggaggacatc gaaatcgagc cacagaccgt cgtcgtcgac
     9421 ggcgtcgaat acctggaagc ccgcgaaccc gtcgagatct actggcacat caccgagccg
     9481 ggccgcgcgg cgcttgaggc cgacaaaccg ctcgagcgcc tgcgcgccct cctggacgaa
     9541 gatgccgcct acgcgcccat ctaccagcgc atcctccgct tgtgcacagc agacggcggc
     9601 gccacgacac ccgccatcaa caacgcggtc gaccacaacc cgctggtgca gaagccgcgc
     9661 ttctacgcgc cccacttcgt cgaccgcctg gagaaatgcg acgcgctggc ctggaagaag
     9721 gcgtggtgca tcaccgacat cggacgcgcc ggcctcgata tgctggcaga cgtcgtagac
     9781 gagaacgcgc ccgccaccca gcccgaaacc cccgcaaccc ctgatcccgc cgcatccaag
     9841 gaggactaac catgaccgaa gcaaccgcgc ccgcaaccga gaccctcgcc gacgaagcga
     9901 ccatgcagga gctcgtcaaa ctgaacgagc agcgcgccgc cacgtacggc ctgctgtcgc
     9961 gcctgtaccg cgtggaggtg gaccagcccc ttctcgacga gctgcgcagc atgcgcttcc
    10021 ccgccaagac gggcaacgag aacgtggacg agggctaccg cctgctggcc acgtacctga
    10081 gcaacacctg ggacaacagc gtcaccgacc tcgccatcga ctacgtgcgc gtgttcatcg
    10141 gacacggcat cgacgcgttc tcggccgcct acccgttcga atcggtgtac acctccgaga
    10201 agcgcctgct catgcaggaa gcacgcgacg aggtgctggc catctaccgc agcgcaggcc
    10261 tcgacaagca ggacacatgg aaagagggcg aggatcatat cgcgctcgag ctggaattcg
    10321 agcagatcat ggccacgcgc acggtcgaag cgctgcgcaa aggcgacgaa gacgaggccg
    10381 tcgcgctgct gaccacgcag aagaactttc tcgaagacca tctggcggca tgggctccca
    10441 tgatgacctc cgacatgaag cgattcgcgc aaaccgactt ctaccggggc ttggcgtact
    10501 tgacggacgg gttcctccaa accgaccaaa cgttcctcga cgacgtgctg gccgaagact
    10561 aactgcacag gatcgaaaga aggagcgatg gacaacaagc aggagaaaac ctacgccggc
    10621 atgacgcgac gcacgctgtg cctgggcgca ggcggcgctg cggtcatgct aggtctaggc
    10681 ggattgaagt acgcgggatc gcaaccgctc gtgcgcccgc cgggaggcca ggacgaggaa
    10741 cggctcatct cggcctgcat ccgctgcgag aagtgctacg agatctgccc gcgcgacgtc
    10801 atcgcgcccg cccacctcga ggacggtgtg ctgaacatgc gcacccccac gttcgacttc
    10861 tcggccaact ggtgcgactg gtgcgccgaa gagcacgacg gcaccccgtt gtgcgtgagc
    10921 gcctgcccca ccgaagcgct caagctgccc gacgacgcca cggccgagaa caccatcatc
    10981 ggcaaagccg ttatcaacac ggactggtgc ttggcgtaca agctgatcgg atgccggttt
    11041 tgctacgacg cctgtcccta cgaggccatg gagctcgacg agaacaaccg ccccgtcgtg
    11101 atcgaggaca agtgcaacgg ctgcggcgcg tgcgagagcg tgtgcgtctc gctgcagaac
    11161 ggctccatct ccgaaggagc caccgcgcgc gccatcgtcg tccacccgct tgacgaagtt
    11221 gaaggataag gtgccctcat gaagaagaac tccaagaagc tgcgcaccct cgtcgcactg
    11281 gccatcgtgg ccgtcgtcgc cgtcggcttc gtcacgaacg tcgggatcgg caccatgtcg
    11341 gcaccgggca tctgggacat ctccatccta tgcccgctgg gcgccttggg aacgatgctc
    11401 gcctccaaga tgatggtgcc gcgcgccgta atctcgctga tcgtgatggt gctgctcatc
    11461 gttctgttcg cccgcgcgtt ctgcggatgg atttgccccg tgccgctcgt gcagaagttg
    11521 cgcggcttgt tcgcgaagaa gcaacccgtc gaacgcggcg acgatgccga tcctgccgcg
    11581 gcgccgctca cgaaagtcga gatcgacgcg ctgggcgttt catgctcgaa ggggtcgaag
    11641 aatggcagcg gatgcgcgtc ctgcgcctcc aagcgcggag ccgcgctcga tgctcgccac
    11701 ttcgtgctgg gcggcgcgct ggcgtccacc ttcatctttg gattcccggt gttctgcctc
    11761 gtctgcccca tcggactcac gttcgccacc atcctgctgg tcatcaacct gttctcacac
    11821 ggcgatgtca cctggtcgct catcgtggtg cccgcgctgc ttatcgccga ggtgctgctg
    11881 ttcaagaagt ggtgccataa gctgtgcccg ctttcggcgt tcatgagcct gatcgcgaag
    11941 gcgaacaaga ccttcgtgcc cacgatcgac gatgcgaagt gcctggaaac ggcgaagggc
    12001 ggcgcgtgcg gggcctgcgg ccgcgtctgc gaggagggca tcgacccgcg ccacccgcag
    12061 ctttcgcagg cggcatggag cgagtgcacc aagtgccgcg cctgcgtgga cgcatgccct
    12121 gcgaacgcga tcaccatgcc gctgctgccg cggcgaacgc cgtctaccgc agaacctgtc
    12181 gccgtgccaa cgaacgccga gaagatcgac gcggagaatg cgcgccccgt tccgtaaagc
    12241 ccctcctgcg cgacccggct cgcagctgca gcggtctggt cgcgcatccg gaatcgggcc
    12301 gagcccgacc ccgggcaaaa ggcggcttgg caagggcttt gggggacgag ccgccttttg
    12361 cccgggagcg acatccccgc tccccctccc tgcgaaaaga gggatgccgc ttccggcctt
    12421 catctgacaa ccacgacgtg ggaggacgaa aacccatggc atacgaaacg acaagcgaat
    12481 cgaccgaggc cctgctcgtc accggaggcg tcggcaccgg caaaacgcaa cgtctcgtcg
    12541 agcgcgctgc cgcgctgctg gccgaaaacg ccgccgcggg caacgtgctc gtgctgtgcg
    12601 cgacgcccca ggccgcacgc ctgttctcgg agcggttgac agcccgcgtc ggtgccaagc
    12661 gcgctgccgg cgcaaccgtc gccaccgcac gcgccttagc gctcgacgtg ctggccgacg
    12721 acggcgcgcg ccgctggtcg gggcgcgagc cgcgactact gaccgccttc gaagagacgt
    12781 tcctcatgga ggacatgaag gtgagcgggc tgcggccgaa gcggctgcgc gagatgctca
    12841 agttcttcta ccgctcgtgg accgagctgg cggacgacga tcccgattgg ctgctaccgg
    12901 gcgaggaggc cgatgtccat acgctgttga aagagaacct ggtcttcatg cgcgccgtca
    12961 tagaacccga agcggcgaac cttgctgcac gctacctccg agaacacgac gaagcgcggg
    13021 cggcgcattc ctacgcgcac gtgctgatgg acgactacgc ctgcgcaagc cgtgcctcgc
    13081 agacgctggc gaatctcgtc gcaacctgct cgatcaccgt ggcgggcgac gcggaaacct
    13141 gcatcgagac atacgattcg tacccctatg ccgccggaat cgacgaattc ctcgcaacgc
    13201 atcccgatgc tgcttgcgag cacctggatg ccgacgcgcc cgatgccgtc gaacgggtcg
    13261 ctctggcaag cgacacgccc gaagccgagt tcgaacaggt ggcgcgcacg gtggccgaag
    13321 ccgtcgccgc aggaacgccc gcacggcaga tcgtcgtggc cgtaccgaac agcgtgtggt
    13381 cgcgcaacat cgcaaccgct ttgcacgcac gaggcgtgcc aaccgaagcg ctcgccatct
    13441 cgcagcccct gcgcggcgac gtgcgggaca acgcccgctg cacgcctgcg cgcatcgcca
    13501 cggccctcaa cctcgtggcg aacccagacg acaccgcggc ttggcggtgc tggtgtggat
    13561 tcggcgacta cctggtgaac agcgcggctt tcgccagcct gcggacttgc gcgaaggagc
    13621 gcagtatcgg cctcgtggac gctttggaga tgctaagcga aaacgactgc gagcacgtag
    13681 tgggcgcgca acgcgtggcg accgcctaca aggacggtcg cgcgctcatc gagcaggtac
    13741 gaggattgcg gggcacggca ctgctggacg agctgacgcg catcgtcacc gacggagcgg
    13801 aatcccaagc gcccgccgtt ctgcgcgagc tctgcctcga gcacggcaac gcagccgatg
    13861 aaaacccgga aagcctttac gatgccgatg ctgcggcgat ggcgcggcgc atggccgagc
    13921 gcctgctcgc gcccacgctg gaggttgccg acgcggtagc cgtcgtcccc tacgacttcg
    13981 cctgcggctt ctcgcccgac ctgctgatcg tcgccggctt cgtgaacggt ttcatcccct
    14041 cccgcgacta tttcgacgcc acggtgaccc cgcccgagcg ccaggagaag atgcgcgccg
    14101 tcgacgaacg acgcgtgcgc gcgctcacag gaaaggccgg ccgcgcggtg gcgctgtcgt
    14161 acttcacgtc caccgacctc gaaagcgccg gcaagctgaa gctcaagatc agtcgcatcc
    14221 gcctcaagaa cggtatccgc acctgcacta tagagcccag cctattcctg gagcaaatcg
    14281 cagcgcagga gtagtaaaaa aaggatcaat tttgaactct ctcccaaagt tgatccctta
    14341 acgatccctg caggagaagg ccatcctgac ggatggcctt tttgaagctt tctagaatcg
    14401 ggggcccccg gggggattct agaacaatgg aagtcctcgt ctcgccctcg aattagcccg
    14461 cttaatgagc gggctttttt tgaattaatt ctcgcgagct ggcacgacag gtttcccgac
    14521 tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agcgcgaatt gcaagctggc
    14581 cgacgcgctg ggctacgtct tgctggcgtt cgggagcaga agagcataca tctggaagca
    14641 aagccaggaa agcggcctat ggagctgtgc ggcagcgctc agtaggcaat ttttcaaaat
    14701 attgttaagc cttttctgag catggtattt ttcatggtat taccaattag caggaaaata
    14761 agccattgaa tataaaagat aaaaatgtct tgtttacaat agagtggggg gggtcagcct
    14821 gccgccttgg gccgggtgat gtcgtacttg cccgccgcga actcggttac cgtccagccc
    14881 agcgcgacca gctccggcaa cgcctcgcgc acccgctggc ggcgcttgcg catggtcgaa
    14941 ccactggcct ctgacggcca gacatagccg cacaaggtat ctatggaagc cttgccggtt
    15001 ttgccggggt cgatccagcc acacagccgc tggtgcagca ggcgggcggt ttcgctgtcc
    15061 agcgcccgca cctcgtccat gctgatgcgc acatgctggc cgccacccat gacggcctgc
    15121 gcgatcaagg ggttcagggc cacgtacagg cgcccgtccg cctcgtcgct ggcgtactcc
    15181 gacagcagcc gaaacccctg ccgcttgcgg ccattctggg cgatgatgga taccttccaa
    15241 aggcgctcga tgcagtcctg tatgtgcttg agcgccccac cactatcgac ctctgccccg
    15301 atttcctttg ccagcgcccg atagctacct ttgaccacca tggcatcagc ggtgacggcc
    15361 tcccacttgg gttccaggaa cagccggagc tgccgtccgc cttcggtctt gggttccggg
    15421 ccaagcacta ggccattagg cccagccatg gccaccagcc cttgcaggat gcgcagatca
    15481 tcagcgccca gcggctccgg gccgctgaac tcgatccgct tgccgtcgcc gtagtcatac
    15541 gtcacgtcca gcttgctgcg cttgcgctcg ccccgcttga gggcacggaa caggccgggg
    15601 gccagacagt gcgccgggtc gtgtcggacg tggctgaggc tgtgcttgtt cttaggcttc
    15661 accacggggc acccccttgc tcttgcgctg cctctccagc acggcgggct tgagcacccc
    15721 gccgtcatgc cgcctgaacc accgatcagc gaacggtgcg ccatagttgg ccttgctcac
    15781 accgaagcgg acgaagaacc ggcgctggtc gtcgtccaca ccccattcct cggcctcggc
    15841 gctggtcatg ctcgacaggt aggactgcca gcggatgtta tcgaccagta ccgagctgcc
    15901 ccggctggcc tgctgctggt cgcctgcgcc catcatggcc gcgcccttgc tggcatggtg
    15961 caggaacacg atagagcacc cggtatcggc ggcgatggcc tccatgcgac cgatgacctg
    16021 ggccatgggg ccgctggcgt tttcttcctc gatgtggaac cggcgcagcg tgtccagcac
    16081 catcaggcgg cggccctcgg cggcgcgctt gaggccgtcg aaccactccg gggccatgat
    16141 gttgggcagg ctgccgatca gcggctggat cagcaggccg tcagccacgg cttgccgttc
    16201 ctcggcgctg aggtgcgccc caagggcgtg caggcggtga tgaatggcgg tgggcgggtc
    16261 ttcggcgggc aggtagatca ccgggccggt gggcagttcg cccacctcca gcagatccgg
    16321 cccgcctgca atctgtgcgg ccagttgcag ggccagcatg gatttaccgg caccaccggg
    16381 cgacaccagc gccccgaccg taccggccac catgttgggc aaaacgtagt ccagcggtgg
    16441 cggcgctgct gcgaacgcct ccagaatatt gataggctta tgggtagcca ttgattgcct
    16501 cctttgcagg cagttggtgg ttaggcgctg gcggggtcac tacccccgcc ctgcgccgct
    16561 ctgagttctt ccaggcactc gcgcagcgcc tcgtattcgt cgtcggtcag ccagaacttg
    16621 cgctgacgca tccctttggc cttcatgcgc tcggcatatc gcgcttggcg tacagcgtca
    16681 gggctggcca gcaggtcgcc ggtctgcttg tccttttggt ctttcatatc agtcaccgag
    16741 aaacttgccg gggccgaaag gcttgtcttc gcggaacaag gacaaggtgc agccgtcaag
    16801 gttaaggctg gccatatcag cgactgaaaa gcggccagcc tcggccttgt ttgacgtata
    16861 accaaagcca ccgggcaacc aatagccctt gtcacttttg atcaggtaga ccgaccctga
    16921 agcgcttttt tcgtattcca taaaaccccc ttctgtgcgt gagtactcat agtataacag
    16981 gcgtgagtac caacgcaagc actacatgct gaaatctggc ccgcccctgt ccatgcctcg
    17041 ctggcggggt gccggtgccc gtgccagctc ggcccgcgca agctggacgc tgggcagacc
    17101 catgaccttg ctgacggtgc gctcgatgta atccgcttcg tggccgggct tgcgctctgc
    17161 cagcgctggg ctggcctcgg ccatggcctt gccgatttcc tcggcactgc ggccccggct
    17221 ggccagcttc tgcgcggcga taaagtcgca cttgctgagg tcatcaccga agcgcttgac
    17281 cagcccggcc atctcgctgc ggtactcgtc cagcgccgtg cgccggtggc ggctaagctg
    17341 ccgctcggac agttcgaggc tggccagcct gcgggccttc tcctgctgcc gctgggcctg
    17401 ctcgatctgc tggccagcct gctgcaccag cgccgggcca gcggtggcgg tcttgccctt
    17461 ggattcacgc agcagcaccc acggctgata accggcgcgg gtggtgtgct tgtccttgcg
    17521 gttggtgaag cccgccaagc ggccatagtg gcggctgtcg gcgctggccg ggtcggcgtc
    17581 gtactcgctg gccagcgtcc gggcaatctg cccccgaagt tcaccgcctg cggcgtcggc
    17641 caccttgacc catgcctgat agttcttcgg gctggtttcc actaccaggg caggctcccg
    17701 gccctcggct ttcatgtcat ccaggtcaaa ctcgctgagg tcgtccacca gcaccagacc
    17761 atgccgctcc tgctcggcgg gcctgatata cacgtcattg ccctgggcat tcatccgctt
    17821 gagccatggc gtgttctgga gcacttcggc ggctgaccat tcccggttca tcatctggcc
    17881 ggtggtggcg tccctgacgc cgatatcgaa gcgctcacag cccatggcct tgagctgtcg
    17941 gcctatggcc tgcaaagtcc tgtcgttctt catcgggcca ccaagcgcag ccagatcgag
    18001 ccgtcctcgg ttgtcagtgg cgtcaggtcg agcaagagca acgatgcgat cagcagcacc
    18061 accgtaggca tcatggaagc cagcatcacg gttagccata gcttccagtg ccacccccgc
    18121 gacgcgctcc gggcgctctg cgcggcgctg ctcacctcgg cggctacctc ccgcaactct
    18181 ttggccagct ccacccatgc cgcccctgtc tggcgctggg ctttcagcca ctccgccgcc
    18241 tgcgcctcgc tggcctgctg ggtctggctc atgacctgcc gggcttcgtc ggccagtgtc
    18301 gccatgctct gggccagcgg ttcgatctgc tccgctaact cgttgatgcc tctggatttc
    18361 ttcactctgt cgattgcgtt catggtctat tgcctcccgg tattcctgta agtcgatgat
    18421 ctgggcgttg gcggtgtcga tgttcagggc cacgtctgcc cggtcggtgc ggatgccccg
    18481 gccttccatc tccaccacgt tcggccccag gtgaacaccg ggcaggcgct cgatgccctg
    18541 cgcctcaagt gttctgtggt caatgcgggc gtcgtggcct gcccgctcta atgcccggtt
    18601 ggcatggtcg gcccatgcct cgcgggtctg ctcaagccat gccttgggct tgagcgcttc
    18661 ggtcttctgt gccccgccct tctccggggt cttgccgttg taccgcttga accactgagc
    18721 ggcgggccgc tcgatgccgt cattgatccg ctcggagatc atcaggtggc agtgcgggtt
    18781 ctcgccgcca ccggcatgga tggccagcgt atacggcagg cgctcggcac cggtcaggtg
    18841 ctgggcgaac tcggacgcca gcgccttctg ctggtcgagg gtcagctcga ccggcagggc
    18901 aaattcgacc tccttgaaca gccgcccatt ggcgcgttca tacaggtcgg cagcatccca
    18961 gtagtcggcg ggccgctcga cgaactccgg catgtgcccg gattcggcgt gcaagacttc
    19021 atccatgtcg cgggcatact tgccttcgcg ctggatgaag tcggccttgg ccctggccga
    19081 ttggccgccc gacctgctgc cggttttcgc cgtaaggtga taaatcgcca tgctgcctcg
    19141 ctgttgcttt tgcttttcgg ctccatgcaa tggccctcgg agagcgcacc gcccgaaggg
    19201 tggccgttag gccagtttct cgaagagaaa ccggtaagtg cgccctcccc tacaaagtag
    19261 ggtcgggatt gccgccgctg tgcctccatg atagcctacg agacagcaca ttaacaatgg
    19321 ggtgtcaaga tggttaaggg gagcaacaag gcggcggatc ggctggccaa gctcgaagaa
    19381 caacgagcgc gaatcaatgc cgaaattcag cgggtgcggg caagggaaca gcagcaagag
    19441 cgcaagaacg aaacaaggcg caaggtgctg gtgggggcca tgattttggc caaggtgaac
    19501 agcagcgagt ggccggagga tcggctcatg gcggcaatgg atgcgtacct tgaacgcgac
    19561 cacgaccgcg ccttgttcgg tctgccgcca cgccagaagg atgagccggg ctgaatgatc
    19621 gaccgagaca ggccctgcgg ggctgcacac gcgcccccac ccttcgggta gggggaaagg
    19681 ccgctaaagc ggctaaaagc gctccagcgt atttctgcgg ggtttggtgt ggggtttagc
    19741 gggctttgcc cgcctttccc cctgccgcgc agcggtgggg cggtgtgtag cctagcgcag
    19801 cgaatagacc agctatccgg cctctggccg ggcatattgg gcaagggcag cagcgcccca
    19861 caagggcgct gataaccgcg cctagtggat tattcttaga taatcatgga tggatttttc
    19921 caacaccccg ccagcccccg cccctgctgg gtttgcaggt ttgggggcgt gacagttatt
    19981 gcaggggttc gtgacagtta ttgcaggggg gcgtgacagt tattgcaggg gttcgtgaca
    20041 gttagtacgg gagtgacggg cactggctgg caatgtctag caacggcagg catttcggct
    20101 gagggtaaaa gaactttccg ctaagcgata gactgtatgt aaacacagta ttgcaaggac
    20161 gcggaacatg cctcatgtgg cggccctcgc gagaattaat tcagataaaa aaaatcctta
    20221 gctttcgcta aggatgattt ctagcgatga ccctgctgat tggttcgctg accgtagact
    20281 ggttggcttg gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg
    20341 ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa
    20401 gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg
    20461 gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgacatc
    20521 gcgcgcgcgc gcgcggatgc tagcggcgcg ccaaaaaaaa gcccgctcat ta
//
