LOCUS       sequence-472383-        4806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 600, Emission:
            650.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Lin MZ, McKeown MR, Ng HL, Aguilera TA, Shaner NC, Campbell RE, 
            Adams SR, Gross LA, Ma W, Alber T, Tsien RY
  TITLE     Autofluorescent proteins with excitation in the optical window for 
            intravital imaging in mammals.
  JOURNAL   Chem Biol. 2009 Nov 25;16(11):1169-79. doi: 
            10.1016/j.chembiol.2009.10.009.
  PUBMED    19942140
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2009.10"; journalName: "Chem Biol"; date: 
            "2009-11-25- 25"; volume: "16"; issue: "11"; pages: "1169-79"
COMMENT     Sequence Label: mNeptune-pBAD
FEATURES             Location/Qualifiers
     source          1..4806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             32..49
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             53..85
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(89..97,98..112)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             128..862
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein derived 
                     from mKate"
                     /label=mNeptune
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTG
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     primer_bind     complement(908..925)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1078..1164
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1256..1283
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1302..1393
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1394..1462,1463..2254)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1612..1631)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2425..3013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2914..2933
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3167..3184
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3199..3339
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3265..3284)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3425..3447
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3603..4481)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4508..4792
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4715..4734
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 ctaacaggag gaattaacca tggggggttc tcatcatcat catcatcatg gtatggctag
       61 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatccgag
      121 ctcgagcatg gtgagcaagg gcgaagagct gattaaggag aacatgcaca tgaagctgta
      181 catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc
      241 ctacgagggc acccagaccg gcagaatcaa ggtggtcgag ggcggccctc tccccttcgc
      301 cttcgacatc ctggctacct gcttcatgta cggcagcaag accttcatca accacaccca
      361 gggcatcccc gatttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac
      421 cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg
      481 cctcatctac aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca
      541 gaagaaaaca ctcggctggg aggccagcac cgagaccctg taccccgctg acggcggcct
      601 ggaaggcaga tgcgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaacct
      661 gaagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctactt
      721 tgtggaccgc agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca
      781 cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca aacttaatgg
      841 catggacgag ctgtacaagt aagaattcga agcttggctg ttttggcgga tgagagaaga
      901 ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc
      961 ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc
     1021 gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa
     1081 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1141 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg
     1201 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag
     1261 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat
     1321 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1381 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1441 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1501 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1561 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1621 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     1681 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1741 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1801 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1861 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     1921 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     1981 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     2041 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     2101 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     2161 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     2221 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2281 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     2341 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2401 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2461 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2521 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     2581 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2641 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2701 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2761 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2821 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2881 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2941 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3001 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3061 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     3121 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     3181 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     3241 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     3301 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     3361 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3421 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     3481 cagatcaatt cgcgcgcgaa ggcgaagcgg catgcataat gtgcctgtca aatggacgaa
     3541 gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc
     3601 aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg
     3661 ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa
     3721 ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc
     3781 tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa
     3841 agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa
     3901 ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg attatccatc
     3961 ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga
     4021 tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca
     4081 aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc cgtattggca
     4141 aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac
     4201 tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc tcctggcggg
     4261 aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt caccaccccc
     4321 tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa
     4381 aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac
     4441 gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca
     4501 ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac
     4561 tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct gtaacaaagc
     4621 gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc
     4681 cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga
     4741 ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt
     4801 tttggg
//
