LOCUS       sequence-472387-        7552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Quayle SN, Chheda MG, Shukla SA, Wiedemeyer R, Tamayo P, Dewan RW, 
            Zhuang L, Huang-Hobbs E, Haidar S, Xiao Y, Ligon KL, Hahn WC, Chin L
  TITLE     Integrative functional genomics identifies RINT1 as a novel GBM 
            oncogene.
  JOURNAL   Neuro Oncol. 2012 Nov;14(11):1325-31. doi: 10.1093/neuonc/nos246. 
            Epub 2012 Oct 16.
  PUBMED    23074196
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/neuonc/nos246"; journalName: "Neuro Oncol"; date: 
            "2012-11"; volume: "14"; issue: "11"; pages: "1325-31"
COMMENT     Sequence Label: pBabe-GW-HA-RINT1
FEATURES             Location/Qualifiers
     source          1..7552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             119..535
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     465..487
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     503..519
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    568..592
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2969..2993)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2998..3024
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3059..3079)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3064..3393
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3244..3379
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3403..4002
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3403..3422)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3899..3919
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4223..4692
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4822..4839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4993..5581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5073..5092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5741..6532,6533..6601))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6364..6383
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6602..6706)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             6737..7206
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    7271..7470
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg
       61 cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg
      121 ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat
      181 cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga
      241 atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac
      301 ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta
      361 catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca
      421 ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg
      481 ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag gcgccggccg
      541 gatcccagtg tggtggtacg tagatcaaca agtttgtaca aaaaagttgg catgctacca
      601 gccggcgaga tcggcgcctc tcctgcagcc ccgtgctgct ctgaaagtgg tgacgaaagg
      661 aagaacctcg aggagaaaag tgacataaat gttacagttc ttattggaag taaacaagtc
      721 agtgaaggta cagataatgg tgatctccct tcttatgtgt ctgcattcat agaaaaggaa
      781 gttggaaatg accttaaatc tttaaagaaa cttgataaac tcatagaaca gaggacagta
      841 agtaaaatgc agttagaaga acaggtactt acaatttcat cagaaattcc taaaagaatt
      901 cgaagtgcct taaaaaatgc agaagaatca aagcaatttc ttaatcagtt tctggagcag
      961 gaaactcatc tcttcagcgc cattaacagc catttgctga ctgcgcaacc ttggatggac
     1021 gatcttggaa ccatgattag ccagattgaa gagatcgaac gtcatcttgc ttaccttaaa
     1081 tggatttcac aaattgaaga actaagtgat aacattcagc aatatctgat gaccaataat
     1141 gtaccggagg cagcctccac tctagtgtct atggcagaac ttgacattaa acttcaggaa
     1201 tcatcttgta ctcatcttct tggtttcatg agagccacag ttaaattctg gcataaaatt
     1261 ctcaaggaca agcttacaag tgattttgag gaaattttag cacagcttca ttggccattc
     1321 atcgcacccc ctcaatcaca aactgttggc ttaagtcgac ctgccagtgc cccggagata
     1381 tacagttacc tggagacact gttttgtcag cttttgaaac tacaaacctc agatgaatta
     1441 cttactgagc caaagcaact cccagaaaaa tactctcttc ctgcctcccc ttctgtcatc
     1501 ctgcccatcc aggttatgct gactcctctt cagaagaggt tcaggtatca cttcagaggg
     1561 aaccggcaga ctaatgtgtt aagcaagcca gaatggtact tggctcaagt acttatgtgg
     1621 attggaaacc atactgaatt tctggatgag aagattcagc caatattaga caaagtaggc
     1681 tctttggtaa acgcaaggct tgaattttct cggggcctta tgatgctggt tcttgagaag
     1741 ttagccactg atattccttg tctgctatat gatgacaatc tcttctgtca tttggtggat
     1801 gaagtactct tgtttgaaag ggagctacac agtgttcatg gctatcctgg cacttttgct
     1861 agttgtatgc atattctatc agaggaaacc tgttttcaga gatggttgac ggtggagaga
     1921 aaatttgctc ttcaaaaaat ggactcaatg ctttcctcag aagctgcctg ggtatcgcaa
     1981 tataaggata tcactgacgt ggatgaaatg aaagttccag attgtgcaga aacttttatg
     2041 actctactct tggttataac tgacaggtat aaaaatcttc ccacagcttc ccgaaagctt
     2101 cagttcctgg agttacagaa ggacttagta gatgatttta ggatacgatt aacacaagtg
     2161 atgaaagaag agactagagc ttcccttggc tttcgatact gtgcaattct taatgctgtg
     2221 aactacatct caacagtact agcagattgg gctgacaatg ttttctttct acaacttcaa
     2281 caggctgcac tggaggtgtt tgcagagaat aatactctga gtaaattgca gctaggacag
     2341 ctagcctcta tggagagctc tgtctttgat gacatgatta acctcttaga acgtttaaag
     2401 catgatatgt tgacccgtca agtagaccac gtttttagag aagttaaaga tgctgcaaaa
     2461 ttgtataaaa aagaaagatg gttgtccttg ccatctcagt cagagcaggc agtgatgtcc
     2521 ctgtccagtt cggcttgccc gttgctgctg acgttacgag accatttact tcagttggag
     2581 cagcagcttt gtttctcctt atttaaaatt ttctggcaaa tgcttgtaga gaagctggat
     2641 gtatacatct accaagaaat aattcttgct aatcacttca atgaaggagg agcagcccag
     2701 ctgcagtttg atatgactcg gaatcttttc cctttgtttt ctcactattg caagagacca
     2761 gaaaattatt ttaaacatat aaaagaagcc tgtattgttt tgaatttgaa cgtcggttct
     2821 gcactactgc tgaaagatgt actgcagtca gcttcagggc agcttcctgc cacagcagca
     2881 ttaaatgaag ttggaattta caaactggct caacaagatg ttgagattct acttaatttg
     2941 aggacaaatt ggcctaatac tggaaaatac ccaactttct tgtacaaagt ggttatgtat
     3001 ccatatgatg ttccagatta tgcttgagat aattcgccag cacagtggtc gaccctgtgg
     3061 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3121 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3181 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3241 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3301 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3361 ggaggctttt ttggaggcct aggcttttgc aaaaagctta ccatgaccga gtacaagccc
     3421 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     3481 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     3541 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     3601 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     3661 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     3721 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     3781 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     3841 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     3901 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     3961 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     4021 cagcgcccga ccgaaaggag cgcacgaccc catgcatcga taaaataaaa gattttattt
     4081 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     4141 gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca
     4201 aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     4261 tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata
     4321 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     4381 tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     4441 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     4501 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct
     4561 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     4621 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     4681 gggggtcttt cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta
     4741 cattaaatgg ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     4801 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     4861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     4921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5341 agcagccact ggtaacagga ttagcagagc taggtatgta ggcggtgcta cagagttctt
     5401 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     5461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     5641 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccgca
     5701 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     5761 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     5821 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5881 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     5941 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6001 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6061 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6121 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6181 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6241 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6301 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     6361 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6421 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6481 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6541 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     6601 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     6661 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     6721 aagtgccacc tgcagcctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     6781 cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     6841 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc
     6901 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
     6961 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
     7021 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt
     7081 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
     7141 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
     7201 ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc
     7261 accgggaggc aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga
     7321 ctgattttat gcgcctgcgt cggtactagt tagctaacta gctctgtatc tggcggaccc
     7381 gtggtggaac tgacgagttc tgaacacccg gccgcaaccc tgggagacgt cccagggact
     7441 ttgggggccg tttttgtggc ccgacctgag gaagggagtc gatgtggaat ccgaccccgt
     7501 caggatatgt ggttctggta ggagacgaga acctaaaaca gttcccgcct cc
//
