LOCUS       sequence-472398-        6537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express the full-length human SAE1:SAE2 heterodimer ( where SAE1 is 
            untagged and SAE2 contains an N-terminal tag : 
            MGSSHHHHHHSQDADLNSRVD, derivated from the Addgene plasmid #52258 
            pSUMO1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6537)
  TITLE     Production and purification of SUMO-UBC9 and SUMO-RANGAP1CTD
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSUMO1-del
FEATURES             Location/Qualifiers
     source          1..6537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(262..309)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     305..323
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(3362..3379)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    3435..3459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(3459..3478)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3460..3478)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3524..3601
                     /gene="lacI"
                     /label=lacI promoter
     CDS             3602..4684
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(3621..3640)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        5745..5836
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5837..6537,1..91)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
       61 aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgaggggc
      121 cgcaagatcc ggccacgatg acccggtcgt cggttcaggg cagggtcgtt aaatagccgc
      181 ttatgtctat tgctggttta ccggtttatt gactaccgga agcagtgtga ccgtgtgctt
      241 ctcaaatgcc tgaggtttca gcaaaaaacc cctcaagacc cgtttagagg ccccaagggg
      301 ttatgctagt tattgctcag cggtggcagc agcctagccc ttggggccaa ggcactccac
      361 aatcccattc cccttcatgc catcgaagaa gaagaagttg ttgtgaggag ggtcccgctg
      421 agacagggcc ttcacaattt cctgtgccaa aatccctcca accaccgcac acactggggc
      481 catctcggag aagcagtacc tgacaaagtc ctcaggaagc aggtcaggac taatacccag
      541 tgagtcaagc acatcatttc gtatctggag caacaactca gaatcttcct catatgtatc
      601 agaactggga tctcttcctt tatctgtacg gaactttaag agcacttgaa ggagaaagta
      661 gtcggaggtc gtgcgcttca gagcagcctt tgctttctca ctgctccagt ccacctccag
      721 ggcttcttta acagggcaga agaccacctt ctttttgacc atcgttgtct cagaagaatc
      781 aagttttgct ctcttggtgt cgggcccatc ttctactcct tggctaactt tggcaacttt
      841 agttttctcc tctacaaact catgctctcc tagattggca aatgtgtatc catggtagcc
      901 aaaaacatct cctgtaaaga acttgatgct atttttgtga cagatctggt caactttaac
      961 tatgacatcc ctggagcagc aagtcagaca cacagcatcg aattgagtga aaaatgactc
     1021 tggtttcttc tctatatcct cagtgtccac cttcacatcc accatggggt tgagattctg
     1081 agctcgctcc aaagaggctt cagccctatt tcggccaaca gacccagtac gaatcaagaa
     1141 ctgagctccg ggatcttctg gagttacctg ttcgtgatcc agcatggtca gtcctttcac
     1201 tcctgccaag atgagattct tggcaatttc agccccaagt cctttcaagc cgacaagaag
     1261 cacccgagag gcccgcagcc gtttctgggc ctccagtccc cacaggcgga tctgccggtc
     1321 atactgtgcc gcctcctcct cgctaatgcc gccgccagcc tcctccttct ccaccatagt
     1381 taattcctcc ttttaatgcg gccgctcaat ctaatgctat gacatcatca agctcttcct
     1441 tctgttctat acgtgacctc tttgcactga gattctcttt ctcatctaat ttcctcttgc
     1501 ggcttctctc ttcttcactg acgtcggcat tatttgaaga atcttcttca tccgaatcaa
     1561 ctatgagaac gtcatcttgc tcttgagctg tggaggtgga gggctgagct ccatcatcac
     1621 tgccattggt tatgcttttg gcagcatctt cagcttgttt gggccccact ttttccggag
     1681 catcaccaac aacttcaaat tcaacgtcct ttcctaggtc ttcactatga aggatgttga
     1741 tcaataaagt atagtcctgg aggaagtcat ctgcttgaag ccggctgcca tttctaattc
     1801 caaattctga caacttcttg tgattattag cttccgtctc tccctcttcg gaagatatta
     1861 ggattgttcc tttcccatct tcaatttgga catctggtgc taccatagca aatttttctt
     1921 tcactatctt gtcttgtaag gtgagaacag tcactttatg gacattcagc cgcacagtca
     1981 cctctggctt gctggcacat acataacaat tggggttggg aggatccagt gcacaaggca
     2041 caagaagctt ctttcttggg tttggttgtt tattcaaaaa aattgttctg cactggtcta
     2101 tttttcctga taaaatcttc aatccttcca atactatcaa cccagcaatt actgcattag
     2161 tagtagcaat agcaggaata atgttccctg ccattgattt gatatcaaat ctactcttca
     2221 tattcatact gaaaatatgc atcctgaggt ttgcagcaga ggtgacaaaa tccattgcag
     2281 atgggtcatc cttatcccat atgagctcag ctccatcccc cttttctgct aaatgaactc
     2341 tcaaagtctc gatgctcttt gaaaaaagac gtgcatagct ctttacatct agaacctgct
     2401 ggtctttcag gcctaactgg ggttcattct gttgatctga tgcattcgtt tcttctcctt
     2461 gactttgtac ttcagcccag tccaacggaa ctggaggttt ccttttccgc catagtttgt
     2521 ccattgtcaa cagatacctg atgtcatctt taaaaagctt ggtaaaaagt ttaactggat
     2581 catatccagt tgatttagcc cattccttag tagaaatacg tttaatgtca ccatcttcat
     2641 tagatgctct agctctggct tcggcttccg ttggttccca ggcagcttca gggtcagctc
     2701 tgtcaggaga tacttcttga tcagcatctt cttccccaaa caactggttg aacaagtact
     2761 ttgcccaaac gatgcaatgt ataggttctg aaggtgtgtt acgaattgta cagccaggaa
     2821 aggttctctg ggtcggctta ggatgacact cataacactc ggtcacaccc tttttgatag
     2881 tagttacttg tccaagatac ccagctgttc cactttcaat aagaggaaca tcagctgcca
     2941 ggcacattct attaacatgg tttcgggcag ctctgttatc taaagcattc ataaccagta
     3001 taaactgtcg gaaaaattcc acattatagt cagggttcat gatgctgtca tggtaggcaa
     3061 cgatattagc tttcgggtaa aactgcagta cactttcctt ggcaacctgt gcctttgatc
     3121 ttccaacatg tttcttttga aacaaaaact gtctgttgag gttgcttaca tcaatagtat
     3181 ccagatcaat caggtcgatg tgggagaaac cggtgagcac gagattcttg aggagctcgc
     3241 agccgatgcc gcccgccccc accaccagca cccggccccc ggccaccgcc tcagccagct
     3301 cccggggcag cccccgcgac agtgccatgt cgacccggga attgagatct gcatcctggc
     3361 tgtggtgatg atggtgatgg ctgctgccca tggtatatct ccttattaaa gttaaacaaa
     3421 attatttcta caggggaatt gttatccgct cacaattccc ctatagtgag tcgtattaat
     3481 ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg
     3541 caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa
     3601 tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
     3661 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
     3721 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
     3781 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
     3841 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
     3901 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
     3961 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
     4021 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
     4081 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
     4141 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
     4201 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
     4261 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
     4321 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
     4381 cgggctgcgc gttggtgcgg acatctcggt agtgggatac gacgataccg aagacagctc
     4441 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
     4501 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
     4561 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     4621 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4681 gtgagcgcaa cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga
     4741 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactaaca
     4801 tgagaattac aacttatatc gtatggggct gacttcaggt gctacatttg aagagataaa
     4861 ttgcactgaa atctagagcg gttcagtaga aaagatcaaa ggatcttctt gagatccttt
     4921 ttttctgcgc gtaatctttt gccctgtaaa cgaaaaaacc acctggggag gtggtttgat
     4981 cgaaggttaa gcagttcccc aactgactta accttcgaaa aaaccacctg gggaggtggt
     5041 ttgatcgaag gttaagtcag ttggggaact gcttaaccgt ggtaactggc tttcgcagag
     5101 cacagcaacc aaatctgtcc ttccagtgta gccggacttt ggcgcacact tcaagagcaa
     5161 ccgcgtgttt agctaaacaa atcctctgcg aactcccagt taccaatggc tgctgccagt
     5221 ggcgttttac cgtgcttttc cgggttggac tcaagtgaac agttaccgga taaggcgcag
     5281 cagtcgggct gaacggggag ttcttgctta cagcccagct tggagcgaac gacctacacc
     5341 gagccgagat accagtgtgt gagctatgag aaagcgccac acttcccgta agggagaaag
     5401 gcggaacagg tatccggtaa acggcagggt cggaacagga gagcgcaaga gggagcgacc
     5461 cgccggaaac ggtggggatc tttaagtcct gtcgggtttc gcccgtactg tcagattcat
     5521 ggttgagcct cacggctccc acagatgcac cggaaaagcg tctgtttatg tgaactctgg
     5581 caggagggcg gagcctatgg aaaaacgcca ccggcgcggc cctgctgttt tgcctcacat
     5641 gttagtcccc tgcttatcca cggaatctgt gggtaacttt gtatgtgtcc gcagcgcccg
     5701 ccgcagtctc acgcccggag cgtagcgacc gagtgagcta gctatttgtt tatttttcta
     5761 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5821 ttgaaaaagg aagagtatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     5881 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     5941 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     6001 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     6061 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     6121 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     6181 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     6241 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     6301 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     6361 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     6421 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     6481 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggaca
//
