LOCUS       sequence-472415-       11248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MCU tetrameric fusion. Only the initial MCU has a mitochondrial 
            targeting sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   Rai NK, Eberhardt DR, Balynas AM, MacEwen MJS, Bratt AR, Sancak Y, 
            Chaudhuri D
  TITLE     Mechanism of MCUB-Dependent Inhibition of Mitochondrial Calcium 
            Uptake.
  JOURNAL   J Cell Physiol. 2025 Apr;240(4):e70033. doi: 10.1002/jcp.70033.
  PUBMED    40227803
REFERENCE   2  (bases 1 to 11248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Physiol."; date: "2025-04"; volume: "240(4):e70033. doi"; pages: " 
            10.1002/jcp.70033"
COMMENT     Sequence Label: MCUaaaa-Flag-pLYS1
FEATURES             Location/Qualifiers
     source          1..11248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             complement(2742..2753)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4257..4280
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4331..4448
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4497..5007
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4926..4944
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5029..5628
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5029..5048)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5525..5545
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5756..5989
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6061..6182
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6098..6117)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6152..6171
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6222..6357
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6377..6396)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6378..6396)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6406..6423)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6406..6422)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6415..6437)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6564..7019
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6651..6670)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6861..6882
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7045..7149
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7150..7218,7219..8010)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7368..7387)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8181..8769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8670..8689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8923..8940
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9057..9078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9093..9098,9099..9116,9117..9123)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9131..9147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9136..9158
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9155..9171
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9155..9171
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9190..9210
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9192..9210
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9238..9464
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9465..9645
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9692..9817
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10310..10543
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10728..10772
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     11070..11090
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcatggcggc cgccgcaggt agatcgctcc tgctgctcct ctcctctcgg ggcggcggcg
      421 gcgggggcgc cggcggctgc ggggcgctga ctgccggctg cttccctggg ctgggcgtca
      481 gccgccaccg gcagcagcag caccaccgga cggtacacca gaggatcgct tcctggcaga
      541 atttgggagc tgtttattgc agcactgttg tgccctctga tgatgttaca gtggtttatc
      601 aaaatgggtt acctgtgata tctgtgaggc taccatcccg gcgtgaacgc tgtcagttca
      661 cactcaagcc tatctctgac tctgttggtg tatttttacg acaactgcaa gaagaggatc
      721 ggggaattga cagagttgct atctattcac cagatggtgt tcgcgttgct gcttcaacag
      781 gaatagacct cctcctcctt gatgacttta agctggtcat taatgactta acataccacg
      841 tacgaccacc aaaaagagac ctcttaagtc atgaaaatgc agcaacgctg aatgatgtaa
      901 agacattggt ccagcaacta tacaccacac tgtgcattga gcagcaccag ttaaacaagg
      961 aaagggagct tattgaaaga ctagaggatc tcaaagagca gctggctccc ctggaaaagg
     1021 tacgaattga gattagcaga aaagctgaga agaggaccac tttggtgcta tggggtggcc
     1081 ttgcctacat ggccacacag tttggcattt tggcccggct tacctggtgg gaatattcct
     1141 gggacatcat ggagccagta acatacttca tcacttatgg aagtgccatg gcaatgtatg
     1201 catattttgt aatgacacgc caggaatatg tttatccaga agccagagac agacaatact
     1261 tactattttt ccataaagga gccaaaaagt cacgttttga cctagagaaa tacaatcaac
     1321 tcaaggatgc aattgctcag gcagaaatgg accttaagag actgagagac ccattacaag
     1381 tacatctgcc tctccgacaa attggtgaaa aagattctag tctcgagctg cctacagcta
     1441 atggcgacgg cggagcactg gctctggatt ctctgacagt tcttggcgct ggcggcaaca
     1501 acgccaacga tgtcgacgtg acagtggtgt accagaatgg cctgcctgtg atctctgtgc
     1561 ggctgcctag tcggagagag cggtgccagt ttaccctgaa gcctatcagc gatagcgtgg
     1621 gcgtgttcct gcggcagctg caagaggaag atcggggcat tgacagagtg gccatctaca
     1681 gccctgacgg cgttagagtg gctgccagca caggcattga tctgctgctg ctggacgact
     1741 tcaagctggt catcaacgac ctgacctacc acgtgcggcc tccaaagaga gatctgctga
     1801 gccacgagaa cgccgccaca ctgaacgacg tgaaaaccct ggtgcagcag ctgtacacca
     1861 cactgtgcat cgagcagcac cagctgaaca aagagcgcga gctgatcgag cggctggaag
     1921 atctgaaaga gcagctggcc cctctggaaa aagtgcggat cgagatcagc cggaaggccg
     1981 agaagagaac cacactggtt ctgtggggcg gactggccta tatggccaca cagtttggca
     2041 tcctggccag actgacttgg tgggagtaca gctgggacat catggaaccc gtgacctact
     2101 tcatcaccta cggcagcgct atggctatgt acgcctactt cgtgatgacc cggcaagagt
     2161 acgtgtaccc cgaggccaga gacagacagt acctgctgtt cttccacaag ggcgccaaga
     2221 agtccagatt cgacctggaa aagtacaacc agctcaagga cgcaatcgcc caggccgaga
     2281 tggacctgaa gagactgaga gatcccctgc aagtgcatct gcccctgaga cagatcggcg
     2341 agaaggatgg atcccttccc accgcaaatg gtgatggggg agccttggct ctcgattccc
     2401 tgactgttct tggtgcgggc gggaataatg cgaatgatgg taccgtgaca gttgtgtacc
     2461 agaatggctt gcctgtcatt tcagtgcgat tgccaagtcg cagagagcga tgccagttca
     2521 ctctgaagcc tatcagtgac tccgtcggtg tgtttctccg gcagctgcaa gaagaggacc
     2581 gggggatcga ccgggtcgca atttatagcc ctgatggagt tcgcgtagct gcgtctactg
     2641 gcattgacct cttgttgctg gacgatttca agctggtaat caatgatctg acctatcatg
     2701 tccgccctcc taagagggat ttgctttccc atgaaaacgc ggcgaccctc aatgacgtaa
     2761 agacccttgt tcaacagctt tacacgacgt tgtgcatcga acagcaccaa cttaataagg
     2821 agcgggaact cattgagaga ctggaggatt tgaaggaaca acttgctccg ttggaaaagg
     2881 tccggataga aatttcacga aaggcagaga agcggacgac gttggtcctc tggggaggac
     2941 tggcatatat ggccacacag ttcggtatac tcgctaggtt gacctggtgg gaatattcat
     3001 gggatattat ggagcccgtt acgtacttca taacatacgg cagcgcaatg gcaatgtacg
     3061 cttattttgt aatgacgagg caggaatacg tatatccgga agcgcgggac cgacaatact
     3121 tgctcttctt ccacaagggt gcgaaaaaga gtaggtttga cctcgaaaag tataatcagc
     3181 ttaaggatgc cattgcccaa gcggagatgg accttaaacg cttgagagat cccttgcaag
     3241 tacacttgcc tctccggcaa attggagaga aggataccgg tctgccaact gccaatggag
     3301 atggcggtgc cctggccctg gacagcctca ccgtgctcgg agccggaggc aataacgcta
     3361 atgatctgca ggtaactgtg gtatatcaaa acggtctccc agtgatctcg gtgcggctgc
     3421 cgagccgccg agagcggtgt cagttcacgc tcaagccaat aagcgattcg gtaggtgtat
     3481 tcttgcgaca actccaggaa gaggacaggg gaattgatcg tgtggctatc tactcgcccg
     3541 atggtgttcg tgtggctgcc agtacaggca ttgacctgct cctcctcgac gattttaaac
     3601 tggtgattaa cgatttgacc taccatgtac gcccccccaa acgtgatctc ctgtcgcacg
     3661 agaatgcagc aacgttgaac gatgtcaaga cattggtaca acagctgtac acgaccttgt
     3721 gcattgagca acaccagctc aataaagaaa gggagctcat cgaacgcttg gaagacttga
     3781 aggaacagct ggctccgttg gagaaggtga ggatagaaat cagccggaaa gccgaaaagc
     3841 ggacgacact cgttctgtgg ggtggcctgg catatatggc gacccagttc ggtattctcg
     3901 cacgactgac gtggtgggaa tatagttggg atatcatgga acctgtgacg tactttatca
     3961 cttatggctc cgctatggct atgtatgcat actttgtaat gacacgtcag gagtacgtct
     4021 atcctgaagc ccgagacagg caatatctgc tctttttcca caaaggcgcc aaaaagtccc
     4081 ggttcgatct cgaaaagtac aatcagttga aagatgctat cgcgcaagcg gagatggacc
     4141 tgaaacggct ccgcgatcct ctccaggtcc acctgccctt gcggcagata ggtgagaagg
     4201 attctagagg tggatctggt ggatctggtg gatctggtgg atctggtgga tctatggatt
     4261 acaaggatga cgatgacaag taaggtaccg aattctcgac ctcgagacaa atggcagtat
     4321 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     4381 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     4441 aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg
     4501 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     4561 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     4621 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     4681 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     4741 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     4801 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     4861 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     4921 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     4981 gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccat gaccgagtac
     5041 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     5101 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     5161 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     5221 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     5281 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     5341 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     5401 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     5461 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     5521 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     5581 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac
     5641 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggtacc tttaagacca
     5701 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     5761 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     5821 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     5881 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     5941 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     6001 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     6061 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6121 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6181 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     6241 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6301 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6361 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     6421 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     6481 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     6541 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6601 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6661 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6721 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6781 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6841 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     6901 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     6961 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     7021 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7081 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7141 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7201 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7261 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7321 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7381 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7441 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7501 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7561 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7621 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7681 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7741 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7801 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     7861 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     7921 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7981 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8041 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8101 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8161 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8221 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8281 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8341 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8401 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8461 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8521 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8581 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8641 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8701 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8761 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8821 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8881 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8941 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     9001 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     9061 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     9121 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     9181 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     9241 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     9301 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     9361 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     9421 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     9481 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9541 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9601 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     9661 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     9721 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     9781 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     9841 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     9901 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     9961 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
    10021 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
    10081 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
    10141 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
    10201 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
    10261 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
    10321 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
    10381 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
    10441 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
    10501 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
    10561 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
    10621 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
    10681 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
    10741 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
    10801 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
    10861 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
    10921 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
    10981 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag
    11041 actagcctcg agcggccgcc cccttcaccg agggcctatt tcccatgatt ccttcatatt
    11101 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
    11161 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
    11221 taaaattatg ttttaaaatg gactatca
//
