LOCUS       sequence-472416-        9191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expressing SARS-CoV-2 KP.3.1.1 spike protein for pseudovirus 
            production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9191)
  TITLE     SARS-CoV-2 variants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: KP.3.1.1
FEATURES             Location/Qualifiers
     source          1..9191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(170..961,962..1030))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     793..812
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1031..1135)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1210..1229)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1401..1780
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1781..1984
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1935..1955
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1981..2005
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(5928..5947)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5972..6020
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6222..6650
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6309..6328)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6519..6540
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6659..6679)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6664..6993
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6844..6979
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6906..6925
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7060..7854
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7114..7133)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7724..7743
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8030..8151
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8067..8086)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8121..8140
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8200..8216)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8200..8216)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8213..8235)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8224..8240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8248..8254,8255..8272,8273..8278))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8293..8314
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8431..8448)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8602..9190)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8682..8701)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
       61 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      181 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
      241 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
      301 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
      361 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
      421 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
      481 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
      541 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
      601 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
      661 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
      721 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
      781 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
      841 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
      901 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      961 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     1021 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     1081 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     1141 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     1201 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     1261 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     1321 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     1381 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     1441 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     1501 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     1561 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     1621 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     1681 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     1741 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     1801 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     1861 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     1921 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     1981 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     2041 tagaagacac cgggaccgat ccagcctccg gactgagctc cttaaggtta actctagaga
     2101 attcggatcc atgtttgtgt tcctggtgct gctgccactg gtgtccagcc agtgtgtgat
     2161 gcctctgttc aacctgatca ccacgaccca atcctacacc aacttcacca ggggagtcta
     2221 ctaccctgac aaggtgttca ggtcctctgt gctgcacctc acccaggacc tgttcctgcc
     2281 attcttcagc aatgtgacct ggttccatgc catctctggc accaatggca ccaagaggtt
     2341 tgacaaccct gtgctgccat tcaatgatgg agtctacttt gccagcacag agaagagcaa
     2401 catcatcagg ggctggattt ttggcaccac cctggacagc aagacccagt ccctgctgat
     2461 tgtgaacaat gccaccaatg tgttcattaa ggtgtgtgag ttccagttct gtaatgaccc
     2521 attcctggac gtctaccaca agaacaacaa gtcctggatg gagtctgaga gtggagtcta
     2581 ctcctctgcc aacaactgta cctttgaata tgtgagccaa ccattcctga tggacttgga
     2641 gggcaagcag ggcaacttca agaacctgag ggagtttgtg ttcaagaaca ttgatggcta
     2701 cttcaagatt tacagcaaac acacaccaat catcggtagg gactttccac agggcttctc
     2761 tgccttggaa ccactggtgg acctgccaat tggcatcaac atcaccaggt tccagaccct
     2821 gctggctctg aacaggtcct acctgacacc tggagactcc tcctctggct ggacagcagg
     2881 agcagcagac tactatgtgg gctacctcca accaaggacc ttcctgctga aatacaatga
     2941 gaatggcacc atcacagatg ctgtggactg tgccctggac ccactgtctg agaccaagtg
     3001 taccctgaaa tccttcacag tggagaaggg catctaccag accagcaact tcagggtcca
     3061 accaacagag agcattgtga ggtttccaaa cgtcaccaac ctgtgtccat ttcacgaggt
     3121 gttcaatgcc accaggtttg cctctgtcta tgcctggaac aggacgagga ttagcaactg
     3181 tgtggctgac tactctgtgc tctacaactt tgcccccttc ttcgccttca agtgttatgg
     3241 agtgagccca accaaactga atgacctgtg tttcaccaat gtctatgctg actcctttgt
     3301 gattaaggga aatgaggtga gtcagattgc ccctggacaa acaggcaaca ttgctgacta
     3361 caactacaaa ctgcctgatg acttcacagg ctgtgtgatt gcctggaaca gcaacaagct
     3421 ggacagcaag cactcaggca actacgacta ctggtacaga tcgctcagga agagcaagct
     3481 gaaaccattt gagagggaca tcagcacaga gatttaccag gctggcaaca aaccatgtaa
     3541 gggaaagggc ccgaactgtt actttccact cgagtcctat ggcttcagac caacctatgg
     3601 agtgggccac caaccataca gggtggtggt gctgtccttt gaactgctcc atgcccctgc
     3661 cacagtgtgt ggaccaaaga agagcaccaa cctggtgaag aacaagtgtg tgaacttcaa
     3721 cttcaatgga ctgacaggca caggagtgct gacaaagagc aacaagaagt tcctgccatt
     3781 ccaacagttt ggcagggaca ttgttgacac cacagatgct gtgagggacc cacagacctt
     3841 ggagattctg gacatcacac catgttcctt tggaggagtg tctgtgatta cacctggcac
     3901 caacaccagc aaccaggtgg ctgtgctcta ccagggtgtg aactgtactg aggtgtctgt
     3961 ggctatccat gctgaccaac ttacaccaac ctggagggtc tacagcacag gcagcaatgt
     4021 gttccagacc agggctggct gtctgattgg agcagagtat gtgaacaact cctatgagtg
     4081 tgacatccca attggagcag gcgtctgtgc ctcctaccag acccagacca agagccgtag
     4141 gagggcaagg tctgtggcaa gccagagcat cattgcctac acaatgagtc tgggagcaga
     4201 gaactctgtg gcttacagca acaacagcat tgccatccca accaacttca ccatctctgt
     4261 gaccacagaa attctgcctg tgagtatgac caagacctct gtggactgta caatgtatat
     4321 ctgtggagac agcacagagt gtagcaacct gctgctccaa tatggctcct tctgtaccca
     4381 acttaagagg gctctgacag gcattgctgt ggaacaggac aagaacaccc aggaggtgtt
     4441 tgcccaggtg aagcagattt acaagacacc tccaatcaag tactttggag gcttcaactt
     4501 cagccagatt ctgcctgacc caagcaagcc aagcaagagg tccttcattg aggacctgct
     4561 gttcaacaag gtgaccctgg ctgatgctgg cttcatcaag caatatggag actgtctggg
     4621 agacattgct gcaagggacc tgatttgtgc ccagaagttc aatggactga ccgtgctgcc
     4681 tccactgctg acagatgaga tgattgccca atacacctct gccctgctgg ctggcaccat
     4741 cacctctggc tggacctttg gagcaggagc agccctccaa atcccatttg ctatgcagat
     4801 ggcttacagg ttcaatggca ttggagtgac ccagaatgtg ctctatgaga accagaaact
     4861 gattgccaac cagttcaact ctgccattgg caagattcag gactccctgt tcagcacagc
     4921 ctctgccctg ggcaaactcc aagatgtggt gaaccataat gcccaggctc tgaacaccct
     4981 ggtgaagcaa ctttccagca agtttggagc catctcctct gtgctgaatg acatcctgag
     5041 cagactggac aaggtggagg ctgaggtcca gattgacaga ctgattacag gcagactcca
     5101 atccctccaa acctatgtga cccaacaact tatcagggct gctgagatta gggcatctgc
     5161 caacctggct gccaccaaga tgagtgagtg tgtgctggga caaagcaaga gggtggactt
     5221 ctgtggcaag ggctaccacc tgatgagttt tccacagtct gcccctcatg gagtggtgtt
     5281 cctgcatgtg acctatgtgc ctgcccagga gaagaacttc accacagccc ctgccatctg
     5341 ccatgatggc aaggctcact ttccaaggga gggagtgttt gtgagcaatg gcacccactg
     5401 gtttctgacc cagaggaact tctatgaacc acagattatc accacagaca acacctttgt
     5461 gtctggcaac tgtgatgtgg tgattggcat tgtgaacaac acagtctatg acccactcca
     5521 acttgaactg gactccttca aggaggaact ggacaaatac ttcaagaacc acaccagccc
     5581 tgatgtggac ctgggagaca tctctggcat caatgcctct gtggtgaaca tccagaagga
     5641 gattgacaga ctgaatgagg tggctaagaa cctgaatgag tccctgattg acctccaaga
     5701 actgggcaaa tatgaacaat acatcaagtg gccatggtac atctggctgg gcttcattgc
     5761 tggactgatt gccattgtga tggtgaccat aatgctgtgt tgtatgacct cctgttgttc
     5821 ctgtctgaaa ggctgttgtt cctgtggctc ctgttgtaag tgactcgagc catggaagct
     5881 tgatatctaa ctgactgaac cggtggtacc aaacggggga ggctaactga aacacggaag
     5941 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     6001 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     6061 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     6121 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     6181 catagcagat ctgcgcagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     6241 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6301 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     6361 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6421 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6481 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6541 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6601 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     6661 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6721 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     6781 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     6841 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6901 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     6961 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     7021 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     7081 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     7141 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     7201 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     7261 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     7321 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     7381 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     7441 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     7501 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     7561 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     7621 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     7681 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     7741 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     7801 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     7861 ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     7921 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     7981 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     8041 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8101 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8161 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     8221 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8281 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8341 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8401 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8461 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8521 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8581 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8641 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8701 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8761 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8821 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8881 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8941 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9001 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     9061 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9121 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9181 aggatctcaa g
//
