LOCUS       sequence-472419-       10375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector for full length human PLD4 expression (with flag 
            tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10375)
  TITLE     PLD3/4 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBOBI-hPLD4-flag
FEATURES             Location/Qualifiers
     source          1..10375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4684..4707
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          4776..5005
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4988..5007
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5182..5770
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5235..5255)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5653..5664)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6177..6357
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6383..6400)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6389..6613
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6659..7087
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6746..6765)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6956..6977
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7096..7116)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7101..7430
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7281..7416
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7343..7362
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7478..7525
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7544..7918
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8048..8169
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8085..8104)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8139..8158
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8218..8234)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8218..8234)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8231..8253)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8242..8258
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8266..8272,8273..8290,8291..8296))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8311..8332
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8449..8466)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8620..9208)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8700..8719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9379..10170,10171..10239))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10002..10021
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10240..10344)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagaattcgg atccatgctg aagcctcttt ggaaagcagc agtggccccc
     3181 acatggccat gctccatgcc gccccgccgc ccgtgggaca gagaggctgg cacgttgcag
     3241 gtcctgggag cgctggctgt gctgtggctg ggctccgtgg ctcttatctg cctcctgtgg
     3301 caagtgcccc gtcctcccac ctggggccag gtgcagccca aggacgtgcc caggtcctgg
     3361 gagcatggct ccagcccagc ttgggagccc ctggaagcag aggccaggca gcagagggac
     3421 tcctgccagc ttgtccttgt ggaaagcatc ccccaggacc tgccatctgc agccggcagc
     3481 ccctctgccc agcctctggg ccaggcctgg ctgcagctgc tggacactgc ccaggagagc
     3541 gtccacgtgg cttcatacta ctggtccctc acagggcctg acatcggggt caacgactcg
     3601 tcttcccagc tgggagaggc tcttctgcag aagctgcagc agctgctggg caggaacatt
     3661 tccctggctg tggccaccag cagcccgaca ctggccagga catccaccga cctgcaggtt
     3721 ctggctgccc gaggtgccca tgtacgacag gtgcccatgg ggcggctcac caggggtgtt
     3781 ttgcactcca aattctgggt tgtggatgga cggcacatat acatgggcag tgccaacatg
     3841 gactggcggt ctctgacgca ggtgaaggag cttggcgctg tcatctataa ctgcagccac
     3901 ctggcccaag acctggagaa gaccttccag acctactggg tactgggggt gcccaaggct
     3961 gtcctcccca aaacctggcc tcagaacttc tcatctcact tcaaccgttt ccagcccttc
     4021 cacggcctct ttgatggggt gcccaccact gcctacttct cagcgtcgcc accagcactc
     4081 tgtccccagg gccgcacccg ggacctggag gcgctgctgg cggtgatggg gagcgcccag
     4141 gagttcatct atgcctccgt gatggagtat ttccccacca cgcgcttcag ccaccccccg
     4201 aggtactggc cggtgctgga caacgcgctg cgggcggcag ccttcggcaa gggcgtgcgc
     4261 gtgcgcctgc tggtcggctg cggactcaac acggacccca ccatgttccc ctacctgcgg
     4321 tccctgcagg cgctcagcaa ccccgcggcc aacgtctctg tggacgtgaa agtcttcatc
     4381 gtgccggtgg ggaaccattc caacatccca ttcagcaggg tgaaccacag caagttcatg
     4441 gtcacggaga aggcagccta cataggcacc tccaactggt cggaggatta cttcagcagc
     4501 acggcggggg tgggcttggt ggtcacccag agccctggcg cgcagcccgc gggggccacg
     4561 gtgcaggagc agctgcggca gctctttgag cgggactgga gttcgcgcta cgccgtcggc
     4621 ctggacggac aggctccggg ccaggactgc gtttggcagg gcctcgagcc atggaagctt
     4681 atggactaca aagacgatga cgacaaggat atctaactga ctgaaccggt ggtaccgagg
     4741 aattaattcg ctgtctgcga gggccagctg ttggggtgag tactccctct caaaagcggg
     4801 catgacttct gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg
     4861 gcccgcggtg atgcctttga gggtggccgc gtccatctgg tcagaaaaga caatcttttt
     4921 gttgtcaagc ttgaggtgtg gcaggcttga gatctggcca tacacttgag tgacaatgac
     4981 atccactttg cctttctctc cacaggtgtc cactcccagg tccaactgca ggtcgagcat
     5041 gcatctaggg cggccaattc cgccgatcct ggaaaaacat ggagcaatca caagtagcaa
     5101 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     5161 ttttccagtc acacctcaga caatcaacct ctggattaca aaatttgtga aagattgact
     5221 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5281 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5341 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5401 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5461 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5521 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     5581 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     5641 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     5701 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     5761 gcctccccgc ctggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg
     5821 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     5881 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     5941 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     6001 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     6061 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     6121 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     6181 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6241 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6301 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     6361 ccgctttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     6421 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6481 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6541 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6601 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     6661 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6721 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6781 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6841 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6901 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6961 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7021 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7081 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     7141 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     7201 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     7261 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7321 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     7381 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     7441 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     7501 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     7561 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     7621 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     7681 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     7741 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     7801 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     7861 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     7921 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7981 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     8041 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8101 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8161 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8221 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8281 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8341 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8401 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8461 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8521 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8581 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8641 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8701 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8761 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8821 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8881 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8941 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9001 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9061 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9121 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9181 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9241 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9301 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9361 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9421 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9481 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9541 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9601 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9661 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9721 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9781 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9841 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9901 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9961 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10021 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10081 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10141 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10201 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10261 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10321 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
