LOCUS       sequence-472420-        9194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expressing SARS-CoV-2 KP.3 spike protein for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9194)
  TITLE     SARS-CoV-2 variants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: KP.3
FEATURES             Location/Qualifiers
     source          1..9194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1211..1230)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1402..1781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1782..1985
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1936..1956
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1982..2006
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(5932..5951)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5976..6024
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6226..6654
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6313..6332)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6523..6544
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6663..6683)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6668..6997
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6848..6983
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6910..6929
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7064..7858
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7118..7137)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7728..7747
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8034..8155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8071..8090)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8125..8144
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8204..8220)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8204..8220)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8217..8239)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8228..8244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8252..8258,8259..8276,8277..8282))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8297..8318
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8435..8452)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8606..9194)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8686..8705)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1201 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1261 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1321 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     1381 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     1441 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1501 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1561 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     1621 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     1681 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     1741 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     1801 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     1861 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     1921 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1981 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
     2041 atagaagaca ccgggaccga tccagcctcc ggactgagct ccttaaggtt aactctagag
     2101 aattcggatc catgtttgtg ttcctggtgc tgctgccact ggtgtccagc cagtgtgtga
     2161 tgcctctgtt caacctgatc accacgaccc aatcctacac caactccttc accaggggag
     2221 tctactaccc tgacaaggtg ttcaggtcct ctgtgctgca cctcacccag gacctgttcc
     2281 tgccattctt cagcaatgtg acctggttcc atgccatctc tggcaccaat ggcaccaaga
     2341 ggtttgacaa ccctgtgctg ccattcaatg atggagtcta ctttgccagc acagagaaga
     2401 gcaacatcat caggggctgg atttttggca ccaccctgga cagcaagacc cagtccctgc
     2461 tgattgtgaa caatgccacc aatgtgttca ttaaggtgtg tgagttccag ttctgtaatg
     2521 acccattcct ggacgtctac cacaagaaca acaagtcctg gatggagtct gagagtggag
     2581 tctactcctc tgccaacaac tgtacctttg aatatgtgag ccaaccattc ctgatggact
     2641 tggagggcaa gcagggcaac ttcaagaacc tgagggagtt tgtgttcaag aacattgatg
     2701 gctacttcaa gatttacagc aaacacacac caatcatcgg tagggacttt ccacagggct
     2761 tctctgcctt ggaaccactg gtggacctgc caattggcat caacatcacc aggttccaga
     2821 ccctgctggc tctgaacagg tcctacctga cacctggaga ctcctcctct ggctggacag
     2881 caggagcagc agactactat gtgggctacc tccaaccaag gaccttcctg ctgaaataca
     2941 atgagaatgg caccatcaca gatgctgtgg actgtgccct ggacccactg tctgagacca
     3001 agtgtaccct gaaatccttc acagtggaga agggcatcta ccagaccagc aacttcaggg
     3061 tccaaccaac agagagcatt gtgaggtttc caaacgtcac caacctgtgt ccatttcacg
     3121 aggtgttcaa tgccaccagg tttgcctctg tctatgcctg gaacaggacg aggattagca
     3181 actgtgtggc tgactactct gtgctctaca actttgcccc cttcttcgcc ttcaagtgtt
     3241 atggagtgag cccaaccaaa ctgaatgacc tgtgtttcac caatgtctat gctgactcct
     3301 ttgtgattaa gggaaatgag gtgagtcaga ttgcccctgg acaaacaggc aacattgctg
     3361 actacaacta caaactgcct gatgacttca caggctgtgt gattgcctgg aacagcaaca
     3421 agctggacag caagcactca ggcaactacg actactggta cagatcgctc aggaagagca
     3481 agctgaaacc atttgagagg gacatcagca cagagattta ccaggctggc aacaaaccat
     3541 gtaagggaaa gggcccgaac tgttactttc cactcgagtc ctatggcttc agaccaacct
     3601 atggagtggg ccaccaacca tacagggtgg tggtgctgtc ctttgaactg ctccatgccc
     3661 ctgccacagt gtgtggacca aagaagagca ccaacctggt gaagaacaag tgtgtgaact
     3721 tcaacttcaa tggactgaca ggcacaggag tgctgacaaa gagcaacaag aagttcctgc
     3781 cattccaaca gtttggcagg gacattgttg acaccacaga tgctgtgagg gacccacaga
     3841 ccttggagat tctggacatc acaccatgtt cctttggagg agtgtctgtg attacacctg
     3901 gcaccaacac cagcaaccag gtggctgtgc tctaccaggg tgtgaactgt actgaggtgt
     3961 ctgtggctat ccatgctgac caacttacac caacctggag ggtctacagc acaggcagca
     4021 atgtgttcca gaccagggct ggctgtctga ttggagcaga gtatgtgaac aactcctatg
     4081 agtgtgacat cccaattgga gcaggcgtct gtgcctccta ccagacccag accaagagcc
     4141 gtaggagggc aaggtctgtg gcaagccaga gcatcattgc ctacacaatg agtctgggag
     4201 cagagaactc tgtggcttac agcaacaaca gcattgccat cccaaccaac ttcaccatct
     4261 ctgtgaccac agaaattctg cctgtgagta tgaccaagac ctctgtggac tgtacaatgt
     4321 atatctgtgg agacagcaca gagtgtagca acctgctgct ccaatatggc tccttctgta
     4381 cccaacttaa gagggctctg acaggcattg ctgtggaaca ggacaagaac acccaggagg
     4441 tgtttgccca ggtgaagcag atttacaaga cacctccaat caagtacttt ggaggcttca
     4501 acttcagcca gattctgcct gacccaagca agccaagcaa gaggtccttc attgaggacc
     4561 tgctgttcaa caaggtgacc ctggctgatg ctggcttcat caagcaatat ggagactgtc
     4621 tgggagacat tgctgcaagg gacctgattt gtgcccagaa gttcaatgga ctgaccgtgc
     4681 tgcctccact gctgacagat gagatgattg cccaatacac ctctgccctg ctggctggca
     4741 ccatcacctc tggctggacc tttggagcag gagcagccct ccaaatccca tttgctatgc
     4801 agatggctta caggttcaat ggcattggag tgacccagaa tgtgctctat gagaaccaga
     4861 aactgattgc caaccagttc aactctgcca ttggcaagat tcaggactcc ctgttcagca
     4921 cagcctctgc cctgggcaaa ctccaagatg tggtgaacca taatgcccag gctctgaaca
     4981 ccctggtgaa gcaactttcc agcaagtttg gagccatctc ctctgtgctg aatgacatcc
     5041 tgagcagact ggacaaggtg gaggctgagg tccagattga cagactgatt acaggcagac
     5101 tccaatccct ccaaacctat gtgacccaac aacttatcag ggctgctgag attagggcat
     5161 ctgccaacct ggctgccacc aagatgagtg agtgtgtgct gggacaaagc aagagggtgg
     5221 acttctgtgg caagggctac cacctgatga gttttccaca gtctgcccct catggagtgg
     5281 tgttcctgca tgtgacctat gtgcctgccc aggagaagaa cttcaccaca gcccctgcca
     5341 tctgccatga tggcaaggct cactttccaa gggagggagt gtttgtgagc aatggcaccc
     5401 actggtttct gacccagagg aacttctatg aaccacagat tatcaccaca gacaacacct
     5461 ttgtgtctgg caactgtgat gtggtgattg gcattgtgaa caacacagtc tatgacccac
     5521 tccaacttga actggactcc ttcaaggagg aactggacaa atacttcaag aaccacacca
     5581 gccctgatgt ggacctggga gacatctctg gcatcaatgc ctctgtggtg aacatccaga
     5641 aggagattga cagactgaat gaggtggcta agaacctgaa tgagtccctg attgacctcc
     5701 aagaactggg caaatatgaa caatacatca agtggccatg gtacatctgg ctgggcttca
     5761 ttgctggact gattgccatt gtgatggtga ccataatgct gtgttgtatg acctcctgtt
     5821 gttcctgtct gaaaggctgt tgttcctgtg gctcctgttg taagtgactc gagccatgga
     5881 agcttgatat ctaactgact gaaccggtgg taccaaacgg gggaggctaa ctgaaacacg
     5941 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     6001 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     6061 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     6121 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     6181 ctgccatagc agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6241 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6301 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6361 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6421 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     6481 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6541 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6601 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     6661 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6721 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6781 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6841 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6901 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     6961 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     7021 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     7081 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     7141 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     7201 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     7261 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     7321 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     7381 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     7441 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     7501 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     7561 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     7621 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     7681 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     7741 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     7801 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     7861 gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     7921 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     7981 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     8041 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8101 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8161 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     8221 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8281 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8341 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8401 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8461 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8521 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8581 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8641 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8701 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8761 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8821 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8881 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8941 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9001 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9061 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9121 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9181 aaaaaggatc tcaa
//
