LOCUS       sequence-472421-       12434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-Cas9 plasmid for use in Candida albicans. Contains Cas9, 
            NEUT5L integration site, and the sgRNA cloning site (SNR52 
            promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12434)
  TITLE     Efficient and targeted loss of heterozygosity in Candida albicans 
            using CRISPR-Cas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRS118
FEATURES             Location/Qualifiers
     source          1..12434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_RNA        1611..1685
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     1722..1741
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1955..1977
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1969..1986
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1970..1986
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1993..2012
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1993..2011
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2975..3318
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      3919..4116
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(5052..5068)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5053..5069)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(5074..5094)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(5107..9210)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(5107..9210)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        complement(10244..10262)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10283..10299)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10283..10299)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10296..10318)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10307..10323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10331..10337,10338..10355,10356..10361))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10376..10397
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10514..10531)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10685..11273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10765..10784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11444..12235,12236..12304))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12067..12086
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12305..12409)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg aaggacgttg aaggagagaa agtagtaaga
      361 caatatgact ttggacagac atgagataaa agttttaaag gacaagaaaa ggtttgggtt
      421 aaaagatgag tattgtattt ttcaaaatat caatgctttt tctgttcttg ggttagtggt
      481 accaccattg gtgataaaac ctttggggag cctcctccga gctttctctt tgttactttt
      541 tttctcaact gatacttgtc attcttacta caattacttt tttttttttc tccagagtat
      601 atgaaaagat gaattttgat ctcggattca accttttcat agaagtaata ttacttctta
      661 gtaccataat ggaactaaat cctatcaagt gattcagcat tcttcatttc atgtttgatt
      721 tatgaatata tgaatatatt tttctgggta ttattataca aagcgaattg cttttttttc
      781 ttttacttaa ttgttatata caaatttctt tttttgttat aactatttta attaagaatt
      841 tctagtcact tgacacgacc ttggagtgat cttttttctt tcaaattttc aattttgtga
      901 ttcttagtta attattatta aagattatca tgaattttaa tttttaaatt ctaatacacg
      961 tttacttaat tcttctcttc ttctctttct cttctctttc tttcttttta ttccatatat
     1021 cattacaact ataacccact gaattctaca tcgaacaaga gaaacctttt tttttttttt
     1081 tcaaacttga acaaagaaaa gaaaaagaaa gagaaaaagc aatcacagct tattaacgat
     1141 tcgtgtttaa ataatcaatt gaaaggaaaa tagaagattt ttgaaaaaaa gttattggta
     1201 tttattaact taatatatca gtaacatcaa gagaaagaaa ctgaattata aacagagtat
     1261 gtgaagcaat tgcaattgca attattagtg atccagaaaa ctgaattgtg cttgaatacc
     1321 acttgtttag ccggtaaaag tcattggtca ataactatac tcgagtattg cctcatcaaa
     1381 gaaacaatca aatattatag atactcactc catcacgtga taatttcact ggtatggaaa
     1441 agtggaaaat tttataaaaa aaaatttgat gcctttggca tagctgaaac ttcggcccaa
     1501 taggattgga gaatatgttt tcgcagcgtt cttacaatta aattgtggtg gaagttcgag
     1561 acttgcgtaa actattttta atttcgagga agagcattaa tggctcttca gttttagagc
     1621 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     1681 cggtggtgct tttttttgcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     1741 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     1801 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     1861 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     1921 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     1981 gccagtgaat tgtaatacga ctcactatag ggcgaattgg agactaatag gaattgattt
     2041 ggatggtata aacggaaaca aaaaaaagag ctggtactac tttctttaaa attattttat
     2101 tatttgattt tatttaatag tatatattat attttgaacg tagattattt tgttgaaagt
     2161 tgctgtagtg ccattgattc gtaacactaa ttctgtatta gtcattcctc ttgtttgata
     2221 gtatccaaaa aaacggctat ttttttgcaa tcttatttcc tgcatattat acagataaca
     2281 taatgaaaga aaaaatcttt ttttttgttc ttcaatgatg atttcaacca ttcttttaaa
     2341 cattgatcaa ttcctgagca acaaccccat acacactggt ttatatatcg ccccttttac
     2401 agttgaagaa agaaatagaa atagaaatag caaacaaaag atatgacagt caacactaag
     2461 acctatagtg agagagcaga aactcatgcc tcaccagtag cacaacgatt atttcgatta
     2521 atggaactga agaaaaccaa tttatgtgca tcaattgatg ttgataccac taaggaattc
     2581 cttgaattaa ttgataaatt gggtccttat gtatgcttaa tcaagactca tattgatata
     2641 atcaatgatt tttcctatga atccactatt gaaccattat tagaactttc acgtaaacat
     2701 caatttatga tttttgaaga tagaaaattt gctgatattg gtaataccgt gaagaaacaa
     2761 tatattggtg gagtttataa aattagtagt tgggcagata ttactaatgc tcatggtgtc
     2821 actgggaatg gagtagttga aggattaaaa cagggagcta aagaaaccac caccaaccaa
     2881 gagccaagag ggttattgat gttagctgaa ttatcatcag tgggatcatt agcatatcaa
     2941 gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct
     3001 tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc
     3061 catacatccc catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag
     3121 caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc
     3181 gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact
     3241 gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac
     3301 atccgaacat aaacaaccgg atcctacaat tatgtctact actttggatg atactgctta
     3361 tagatacaga acttctgttc caggtgatgc tgaagctatt gaagctttgg atggttcttt
     3421 cactaccgat actgttttta gagttactgc tactggtgat ggtttcactt tgagagaagt
     3481 tccagttgat ccaccattga ctaaggtttt cccagatgat gaatccgatg atgaatccga
     3541 tgctggtgaa gatggtgatc cagattctag aactttcgtt gcttatggtg atgatggtga
     3601 tttggctggt ttcgttgttg tttcttattc tggttggaac agaagattga ctgttgaaga
     3661 tattgaagtt gctccagaac atagaggtca tggtgttggt agagctttga tgggtttggc
     3721 tactgaattc gccagagaaa gaggtgctgg tcatttgtgg ttggaagtta ccaatgttaa
     3781 tgctccagct attcatgctt atagaagaat gggtttcact ttgtgtggtt tggatactgc
     3841 tttatacgat ggtactgctt ccgatggtga acaagctttg tatatgtcta tgccatgtcc
     3901 ataataactc gagtcagtac tgacaataaa aagattcttg ttttcaagaa cttgtcattt
     3961 gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt gatttatatt
     4021 ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa agtaatatca
     4081 tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata ctaacgccgc
     4141 catccagtgt cgacgtttaa acgagctcga attcgtatgg agaatattct caaaaaactg
     4201 ttgaaattgc taaatccgat aaggaatttg ttattggatt tattgcccaa cgtgatatgg
     4261 gtggacaaga agaaggattt gattggctta ttatgacacc tggagttgga ttagatgata
     4321 aaggtgatgg attaggacaa caatatagaa ctgttgatga agttgttagc actggaactg
     4381 atattatcat tgttggtaga ggattgtttg gtaaaggaag agatccagat attgaaggta
     4441 aaaggtatag agatgctggt tggaatgctt atttgaaaaa aactggccaa ttataaatgt
     4501 gaagggggag attttcactt tattagattt gtatatatgt ataataaata aataaataag
     4561 ttaaataaat aattaaataa gggtggtaat tattactatt tacaatcaaa ggtggtcctt
     4621 ctagaatttt atgatggaat gaatgggatg aatcatcaaa caagagaaaa tacccgtgac
     4681 gaaaataata aaataagttc ctctgataca gaagatgaaa acaacaacaa caagatatag
     4741 aaatgccttg ggtggctatt ttatagtctt aactttttaa tgtatatttg ttttgttttt
     4801 ttacataata atactttata aaagctaagc taaattcaag taaaatttca atctctcaaa
     4861 taaaacattt ttctcttttt cttaaattta gttttatata tttataaaat aaacaaagat
     4921 ttttttaaaa aagtaacaag ttatatatgt aataacaaaa agaagaataa caagaataca
     4981 aaaccagatt tccagatttc cagaatttca ctcggatccc ccgggctgca ggaattcgat
     5041 atcaagctta tcgataccgt cgacctcgag tcagactttt cgttttttct taggatccgc
     5101 tcggctatca ccccccaatt gtgacaaatc gattctagtt tcatataaac cagttatgga
     5161 ctgatgaatc aatgtggcgt ctaacacttc ttttgttgaa gtatatctct tacggtcaat
     5221 ggtggtgtca aagtacttaa acgcggctgg agctcccaag ttagtcaatg tgaacaagtg
     5281 gataatattt tcagcttgtt ctcgaattgg tttgtcacga tgcttattat aagcagataa
     5341 aactttatcc aaatttgcat cagccaaaat aactctttta gaaaactcgg agatttgttc
     5401 aattatctca tccaaatagt gtttatgttg ttctacgaat aattgctttt gctcattgtc
     5461 ttcaggggac cccttcaact tttcataatg agatgctaag tataagaaat taacgtactt
     5521 tgaaggcaaa gctaactcgt ttcctttttg caattctcca gctgaagcca acattctctt
     5581 tctcccattt tcaagttcaa agagagaata ctttggcaac ttaataatca aatctttctt
     5641 cacttcttta tagcccttcg cttcgagaaa gtcaattggg tttttctcaa aggatgaacg
     5701 ctccatgatg gtaataccta acaattcttt cacagatttc aattttttgg atttaccctt
     5761 ttcaacttta gccacgacca agacagaata tgcgacagta ggagaatcaa aacctccgta
     5821 tttcttagga tcccaatctt tttttcgggc gatcaacttg tctgagtttc gttttggaag
     5881 aatggattcc ttagagaaac caccagtttg aacttcagtc ttctttacaa tattaacttg
     5941 aggcattgat aaaacttttc taacagtagc aaaatctctc cccttatccc acacaatttc
     6001 accggtttct ccatttgttt caatcaaagg acgttttctg atttccccgt tagccaaggt
     6061 gatttcggtc ttaaagaaat tcatgatgtt ggagtaaaag aaatactttg cggtggcttt
     6121 cccaatttct tgttctgatt ttgctatcat ctttctgaca tcgtagactt tataatcacc
     6181 atacacgaat tcggattcta acttaggata cttctttatt aaggcagttc ctaccactgc
     6241 attcaaatat gcgtcgtgag catgatgata attgttgatc tctctcacct tgtagaattg
     6301 aaaatccttt cggaaatctg acaccaactt tgactttaat gtgatcactt tgacctctct
     6361 gattaatttg tcattttcat catatttagt attcattcgg gaatctaaaa tttgggctac
     6421 atgtttagta atttgtcttg tttcaaccaa ctgacgtttt atgaatccag ctttatctaa
     6481 ttcggataat cctcctcttt cagctttagt taagttgtca aattttctct gagtaatcaa
     6541 ctttgcattt aacaattgtc tccaatagtt cttcattttc ttaaccactt cttctgatgg
     6601 cacattgtct gatttacctc tattcttatc agatcttgtt aaaactttgt tgtcaatgga
     6661 atcatctttt aaaaatgact gaggaactat gtgatcgaca tcgtaatctg aaagtctatt
     6721 gatatccaac tcttgatcta catacatgtc acgcccgttt tgcaaatagt acaagtaaag
     6781 cttctcgttc tgtaactggg tgttttcgac tgggtgttct tttaagattt gagaacccaa
     6841 ctctttaatt ccttcttcta ttcttttcat tctttcacga gaattctttt gtcctttctg
     6901 agttgtttga ttttctctag ccatttctat aactatattt tcaggcttat gacgtcccat
     6961 aactttcacc aactcatcaa caactttaac agtttgtaaa attccctttt tgatagctgg
     7021 agaccctgct aagttggcaa tatgttcatg caatgaatca ccctgtccag aaacctgagc
     7081 tttttgaata tcttccttaa aggtcaaact atcatcatga atcaactgca taaagtttct
     7141 gtttgcaaaa ccatcacttt taaggaaatc taagatggtt ttaccacttt gcttatctct
     7201 aataccatta atcaactttc tagataatct tccccaaccg gtatatcttc ttctttttaa
     7261 ttgtttcatg actttatcgt cgaataagtg agcgtaagtt tttaaacgtt cttcgatcat
     7321 ttctctatcc tcaaacaaag tcaaagtcaa tacaatgtcc tccaagatat cttcgttttc
     7381 ttcattatcc aaaaaatctt tgtctttgat aattttcaac aaatcgtgat aagttcctaa
     7441 actagcatta aatctatcct caaccccact gatttcaacg gaatcgaaac actctatttt
     7501 cttaaaatag tcctctttaa gttgtttgac agtgacttta cgattagttt tgaataacaa
     7561 gtcaacgata gctttctttt gttcaccact taagaatgct ggttttctca taccttcagt
     7621 gacgtatttg acttttgtta attcattata aacagtgaaa tattcatata acaaagaatg
     7681 tttaggtaac accttctcat ttggcaagtt tttgtcgaaa ttggtcattc tctcgatgaa
     7741 tgattgagcg gaggctccct tatcaacaac ttcctcaaaa ttccatgggg tgatagtctc
     7801 ttcagattta cgagtcatcc atgcaaatct agaattacct ctagccaatg gcccgacgta
     7861 ataaggaatt ctgaaagtca aaattttttc aattttttca cgattgtctt tcaaaaaagg
     7921 gtaaaaatct tcctgacgtc tcaagatagc gtgtaactca cctaagtgga tttgatgagg
     7981 gatagaaccg ttatcaaatg ttctttgctt acgaagtaaa tcctctcgat tcaacttaac
     8041 caacaattcc tcagtaccat ccattttttc aagaattggc ttgataaact tataaaattc
     8101 ttcttgacta gcaccaccat caatataacc agcgtaacca tttttagatt gatcaaagaa
     8161 tatttcttta tacttttctg gtaattgttg acgtacaaga gctttcaaca aggttaaatc
     8221 ttgatggtgt tcatcatatc ttttaatcat agaggcggat aaaggagctt ttgtaatttc
     8281 tgtgttgact ctcaaaatgt cacttaacaa aattgcatca ctcaagttct tagctgccaa
     8341 gaataaatct gcatattgat caccaatttg ggcaagaaga ttatctaaat cgtcatcgta
     8401 tgtatcctta gacaattgaa gtttagcatc ctcggccaaa tcaaagtttg atttgaaatt
     8461 tggggtcaaa cccaaagata atgcaattaa gtttccaaac aatccatttt ttttttcacc
     8521 tggcaattgg gcaataagat tttccaaacg tctacttttt gacaatctag cagataaaat
     8581 agctttagca tccacacctg aggcattgat tggattttcc tcgaaaagtt ggttgtaagt
     8641 ttgaaccaat tgaataaaca acttgtcgac atcggaatta tctggattca aatcaccttc
     8701 aattaagaag tgacctctga acttgatcat gtgagccaat gccaaatata taagtcttaa
     8761 gtcagcttta tcagtggagt caactaattt ttttctcaaa tgataaattg taggatattt
     8821 ttcgtggtaa gccacttcat ctacaatatt accaaagatt ggatgtcttt catgtttctt
     8881 atcttcttca actaagaaac tttcttccaa tctatgaaaa aaagaatcat caacctttgc
     8941 catttcatta gaaaagattt cttgcaagta acaaattctg ttctttcttc tggtatatct
     9001 tcttctagct gttcttttca aacgagttgc ttcagctgtt tcaccactgt caaacaacaa
     9061 ggcaccaatt aaattctttt taatggagtg tctatcagta tttcctaaaa ctttgaactt
     9121 tttagaagga accttatatt catcagtgat tacagcccat ccaactgaat tggttccaat
     9181 gtccaaacct atggagtatt ttttatccat tttgaatgat tatatttttt taatattaat
     9241 atcgagataa tgtttctaag aaagaaagaa aaccaggagt gaaaattaga aaaggaaagg
     9301 aaaggaaaaa aagaaaaatc tgaaaatata taaaaaaaaa ttgtttcgtt ggcaataaat
     9361 cttggtgaga acagcgaccg aaagcaaata agaacaaaat atgagtgtat tacgttgaac
     9421 aactaattaa cgtgtgtgta tggatctttt tttctttttt ctctttaacc gactataaac
     9481 aacaaacatt tttgggcagt gcacacacta cttaatatac acagcataaa ttacacgatt
     9541 agaaacaaat tagcttatta aaataaccta atcaaaccga atattttatg gtattatgag
     9601 taaactatat aatataaata gcacacaccc acaacaacaa caaaggaaaa ctaaaaggtt
     9661 ttttcttttt gaaaagatcg ttttctttat tattctctag ttttgacgct gaaatattcg
     9721 ttgtactgtt gcaaatttgt atttggtctg ccttatcttc ttttctacac tttttactta
     9781 tgagctttat aattcacaga taagtttcag gtttgccaga tatacttgct tcactggtta
     9841 ctaaactctc ggtgtacaaa tgacttgctt atgtaagagg ctacttttct agttttgcag
     9901 tcattcattt aaaaacaatt gatcctgaag gctctaaaga catagtaggt ttactttgac
     9961 tgtatatttc cgtggcatgt ttaaaggccc cttggccata ggatattggt ggcggcacag
    10021 taagcattaa aattgttgtt tcgcggaatt acaacatttc ttcttttaca taataatgtc
    10081 actccttttt gattggcata gccgagatct ttttttaaca cagactttaa gctgtttttt
    10141 gccacctgag aacagtttct attttggtcc aaaagaaata ttagactaag tttattataa
    10201 acgaggctgg tgatcttaat agctctggta ccagcttttg ttccctttag tgagggttaa
    10261 tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    10321 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    10381 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    10441 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    10501 gctcttacgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    10561 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    10621 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    10681 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    10741 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    10801 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    10861 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    10921 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    10981 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    11041 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    11101 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    11161 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    11221 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    11281 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    11341 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    11401 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    11461 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    11521 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    11581 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    11641 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    11701 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    11761 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    11821 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    11881 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    11941 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    12001 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    12061 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    12121 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    12181 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    12241 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    12301 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    12361 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    12421 gaaaagtgcc acct
//
