LOCUS       sequence-472427-       10327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector for full length human PLD3 expression (with flag 
            tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10327)
  TITLE     PLD3/4 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBOBI-hPLD3-flag
FEATURES             Location/Qualifiers
     source          1..10327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4636..4659
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          4728..4957
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4940..4959
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5134..5722
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5187..5207)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5605..5616)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6129..6309
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6335..6352)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6341..6565
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6611..7039
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6698..6717)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6908..6929
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7048..7068)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7053..7382
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7233..7368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7295..7314
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7430..7477
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7496..7870
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8000..8121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8037..8056)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8091..8110
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8170..8186)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8170..8186)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8183..8205)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8194..8210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8218..8224,8225..8242,8243..8248))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8263..8284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8401..8418)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8572..9160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8652..8671)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9331..10122,10123..10191))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9954..9973
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10192..10296)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagaattcgg atccatgaag cctaaactga tgtaccagga gctgaaggtg
     3181 cctgcagagg agcccgccaa tgagctgccc atgaatgaga ttgaggcgtg gaaggctgcg
     3241 gaaaagaaag cccgctgggt cctgctggtc ctcattctgg cggttgtggg cttcggagcc
     3301 ctgatgactc agctgtttct atgggaatac ggcgacttgc atctctttgg gcccaaccag
     3361 cgcccagccc cctgctatga cccttgcgaa gcagtgctgg tggaaagcat tcctgagggc
     3421 ctggacttcc ccaatgcctc cacggggaac ccttccacca gccaggcctg gctgggcctg
     3481 ctcgccggtg cgcacagcag cctggacatc gcctccttct actggaccct caccaacaat
     3541 gacacccaca cgcaggagcc ctctgcccag cagggtgagg aggtcctccg gcagctgcag
     3601 accctggcac caaagggcgt gaacgtccgc atcgctgtga gcaagcccag cgggccccag
     3661 ccacaggcgg acctgcaggc tctgctgcag agcggtgccc aggtccgcat ggtggacatg
     3721 cagaagctga cccatggcgt cctgcatacc aagttctggg tggtggacca gacccacttc
     3781 tacctgggca gtgccaacat ggactggcgt tcactgaccc aggtcaagga gctgggcgtg
     3841 gtcatgtaca actgcagctg cctggctcga gacctgacca agatctttga ggcctactgg
     3901 ttcctgggcc aggcaggcag ctccatccca tcaacttggc cccggttcta tgacacccgc
     3961 tacaaccaag agacaccaat ggagatctgc ctcaatggaa cccctgctct ggcctacctg
     4021 gcgagtgcgc ccccacccct gtgtccaagt ggccgcactc cagacctgaa ggctctactc
     4081 aacgtggtgg acaatgcccg gagtttcatc tacgtcgctg tcatgaacta cctgcccact
     4141 ctggagttct cccaccctca caggttctgg cctgccattg acgatgggct gcggcgggcc
     4201 acctacgagc gtggcgtcaa ggtgcgcctg ctcatcagct gctggggaca ctcggagcca
     4261 tccatgcggg ccttcctgct ctctctggct gccctgcgtg acaaccatac ccactctgac
     4321 atccaggtga aactctttgt ggtccccgcg gatgaggccc aggctcgaat cccatatgcc
     4381 cgtgtcaacc acaacaagta catggtgact gaacgcgcca cctacatcgg aacctccaac
     4441 tggtctggca actacttcac ggagacggcg ggcacctcgc tgctggtgac gcagaatggg
     4501 aggggcggcc tgcggagcca gctggaggcc attttcctga gggactggga ctccccttac
     4561 agccatgacc ttgacacctc agctgacagc gtgggcaacg cctgccgcct gctcctcgag
     4621 ccatggaagc ttatggacta caaagacgat gacgacaagg atatctaact gactgaaccg
     4681 gtggtaccga ggaattaatt cgctgtctgc gagggccagc tgttggggtg agtactccct
     4741 ctcaaaagcg ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg aggaggattt
     4801 gatattcacc tggcccgcgg tgatgccttt gagggtggcc gcgtccatct ggtcagaaaa
     4861 gacaatcttt ttgttgtcaa gcttgaggtg tggcaggctt gagatctggc catacacttg
     4921 agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca ggtccaactg
     4981 caggtcgagc atgcatctag ggcggccaat tccgccgatc ctggaaaaac atggagcaat
     5041 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     5101 ggaggaggtg ggttttccag tcacacctca gacaatcaac ctctggatta caaaatttgt
     5161 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5221 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5281 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     5341 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5401 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5461 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     5521 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     5581 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     5641 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     5701 tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga ccaatgactt
     5761 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     5821 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     5881 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
     5941 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga
     6001 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
     6061 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
     6121 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6181 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6241 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6301 tctctagcag ggccgcttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     6361 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6421 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     6481 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     6541 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     6601 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6661 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6721 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6781 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6841 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6901 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6961 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7021 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     7081 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     7141 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7201 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     7261 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7321 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     7381 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     7441 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     7501 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     7561 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     7621 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     7681 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     7741 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     7801 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     7861 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     7921 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     7981 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     8041 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8101 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     8161 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8221 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8281 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8341 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8401 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8461 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8521 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8581 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8641 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8701 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8761 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8821 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8881 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8941 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9001 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9061 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9121 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9181 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9241 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9301 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9361 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9421 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9481 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9541 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9601 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9661 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9721 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9781 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9841 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9901 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9961 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10021 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10081 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10141 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10201 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10261 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10321 tgacgtc
//
