LOCUS       sequence-47243-e         649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HPV 5E6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Spangle J, Ghosh-Choudhury N, Munger K
  TITLE     Activation of cap dependent translation by mucosal Human 
            Papillomavirus E6 proteins is dependent on the integrity of the 
            LXXLL binding motif.
  JOURNAL   J Virol. 2012 May 2.
  PUBMED    22553330
REFERENCE   2  (bases 1 to 649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2012 May 2."
COMMENT     Sequence Label: pNCMV 5E6
FEATURES             Location/Qualifiers
     source          1..649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             40..66
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atggactaca aggatgacga tgacaagctc gatggaggat acccatacga tgttccagat
       61 tacgctgata tcggatccgc tgagggagcc gaacaccaac agaaactgac agaaaaagat
      121 aaggcagaat tacctttaag tattagagac ttagctgaag ccttaggcat ccctgtgatt
      181 gattgtttaa taccttgcaa tttctgtggc aactttctaa attatttgga agcttgtgaa
      241 ttcgactaca aaaggcttag tctaatttgg aaagattatt gtgtgtttgc gtgctgtcgc
      301 gtatgctgtg gcgccactgc aacttatgaa tttaaccaat tttatgagca gacagtgtta
      361 ggaagagata ttgaattagc ttcaggactt tcaatatttg atattgatat caggtgtcaa
      421 acttgcttag catttcttga cattatagaa aagttagatt gctgtggcag aggccttccc
      481 tttcataagg tgaggaacgc ctggaaggga atctgtaggc agtgtaagca tttttatcat
      541 gattggtaag gatccgtcga ggaattcact cctcaggtgc aggctgccta tcagaaggtg
      601 gtggctggtg tggccaatgc cctggctcac aaataccact gagatcttt
//
