LOCUS       sequence-472432-        4004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-HKU1-Hemagglutinin-esterase
FEATURES             Location/Qualifiers
     source          1..4004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1315..1414)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1431..1450)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1432..1450)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1455..1471)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1455..1471)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1902..2693
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2786..3374
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3275..3294
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3528..3545
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3704..3731)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3823..3909)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3958..3980
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3972..3989
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3973..3989
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatcct taattatatt cctgttcttc tacttttgct acggattcaa cgagccactg
      181 aacgtggtaa gccacctgaa tcacgattgg ttcttgttcg gagacagccg ctccgactgc
      241 aatcacatca acaacttgaa gatcaagaac ttcgactacc tggacatcca tccatccctg
      301 tgtaataacg ggaaaatatc ttccagcgca ggggactcaa tcttcaaatc cttccacttt
      361 accagattct acaactatac gggtgagggc gaccagatca tcttctacga aggggtgaac
      421 ttcaacccat accacaggtt caaatgcttc cccaacggaa gcaacgacgt gtggctgctg
      481 aataaagtcc gcttctacag agcactctac tccaacatgg cgttcttcag gtacctgacc
      541 ttcgtggaca tcccctacaa cgtctccttg agtaaattca actcctgcaa gtcagacata
      601 ctgagcttga ataacccaat attcataaac tactccaaag aggtgtactt cacgttgctc
      661 ggctgctccc tgtacttggt gcctctgtgt ctgttcaaga gcaatttctc acaatattac
      721 tacaatatcg acacaggaag cgtgtacggc ttctcaaacg tcgtctaccc cgacctggat
      781 tgcatataca tcagcttgaa gcccgggagt tacaaggtaa gcactacggc tcccttcctc
      841 tctttgccaa ccaaggcact gtgcttcgac aagagcaagc agttcgtccc cgtgcaagtg
      901 gtcgactccc ggtggaataa tgaacgggct tccgacatct cactgtcagt ggcttgccag
      961 ctgccctact gctacttccg gaactcatcc gccaactacg tcggaaaata cgacatcaat
     1021 catggagaca gcggcttcat cagcatcctc agtggcttgc tgtacaacgt gtcctgcatc
     1081 tcctactacg gagtcttcct ctacgacaac ttcacttcaa tatggcctta ctactcattc
     1141 ggcagatgcc ccactagttc cataatcaag caccccatat gcgtatacga cttcctgcca
     1201 atcatcctgc agggaatcct cttgtgcctg gcactgctgt tcgtagtctt cctgctgttc
     1261 cttctgtaca atgacaagtc ccatcttaat taagacgcga tcgcggcctc atgggcccag
     1321 ctttcttgta caaagttggc attataaaaa ataattgctc atcaatttgt tgcaacgaac
     1381 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     1441 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     1501 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     1561 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     1621 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     1681 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     1741 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     1801 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1861 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     1921 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     1981 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     2041 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2101 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2161 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2221 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2281 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2341 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2401 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2461 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     2521 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     2581 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     2641 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     2701 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     2761 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2821 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2881 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2941 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3001 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3061 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3121 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3181 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3241 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3301 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3361 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3421 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3481 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3541 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3601 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3661 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     3721 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     3781 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     3841 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     3901 ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc
     3961 agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
