LOCUS       sequence-47244-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Yao B, Li S, Jung HM, Lian SL, Abadal GX, Han F, Fritzler MJ, Chan 
            EK
  TITLE     Divergent GW182 functional domains in the regulation of 
            translational silencing.
  JOURNAL   Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2.
  PUBMED    21131274
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-04"; volume: "39(7)"; pages: "2534-47. Epub
            2010 Dec 2"
COMMENT     Sequence Label: NHA-GW182
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          101..233
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     216..235
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        278..296
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      300..309
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             306..371
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             408..434
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             414..440
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     protein_bind    492..516
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag tgactctctt
       61 aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca aggttacaag
      121 acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt
      181 ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca caggtgtcca
      241 ctcccagttc aattacagct cttaaggcta gagtacttaa tacgactcac tataggctag
      301 ccaccatgga cgcacaaaca cgacgacgtg agcgtcgcgc tgagaaacaa gctcaatgga
      361 aagctgcaaa cccaccgctc gaggctgccc aagcttacac catggcctac ccatatgatg
      421 ttccagatta cgcttcactc gacgcggatc ccgtcgaatt cacgcgtggt acctctagag
      481 tcgacccatc aacaagtttg tacaaaaaag caggctcttt aaaggaacca attcagtcga
      541 ctggatccgc catggatgct gattctgcct ccagttctga atcagagaga aacatcacta
      601 tcatggcttc agggaacaca ggtggtgaaa aagatggcct tcggaatagc actggacttg
      661 gttcccaaaa caagtttgta gttggtagca gcagcaataa tgtgggccat ggaagtagta
      721 ctgggccatg gggtttttcc catggagcca taataagcac atgtcag
//
