LOCUS       sequence-472441-        7964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AvNIRusk plasmid for controlling bacterial expression by 
            near-infrared light.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Meier SSM, Horzing M, Bohm C, Duthorn ELR, Takala H, Uebe R, Moglich
            A
  TITLE     Engineering NIR-Sighted Bacteria
  JOURNAL   bioRxiv 2025.04.25.650650
REFERENCE   2  (bases 1 to 7964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.04.25.650650"
COMMENT     Sequence Label: pAvNIRusk
FEATURES             Location/Qualifiers
     source          1..7964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     69..87
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(140..157)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(205..237)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(241..258)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(268..285)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             304..326
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(655..674)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        880..957
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             5263..5454
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(5448..5470)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5556..5695
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5611..5630
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5710..5727)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5881..6469)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5961..5980)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6580..6599)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6591..7406
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6591..7406
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6664..6683)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(7498..7953)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7635..7656)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7847..7866
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
      181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg
      241 gctgccgcgc ggcaccaggc cgctgctgtg atgatgatga tgatggctgc tgcccatggt
      301 atatctcctt cttaaagtta aacaaaatta tttctagact ccgttgtgat gacgcattgg
      361 tacgcggtat cgggaggttc gaaaatttcg agcgatatct taaggggggt gccttacgta
      421 gaaccccgta ggtcatgccc gaggccggtc ctggatggcg cggcggatac gcttgagcag
      481 gttttcgtcg agaagcggct tcaaaaccac gtcttttacg ccggcctcgg cggcccgggt
      541 cgagatgttt tcgtccggat agccggtgat caggatcacg ggcgtagatc tcgatcctct
      601 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
      661 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
      721 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
      781 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
      841 taatgcagga gtcgcataag ggagagcgtc gagatcccgg acaccatcga atggtgcaaa
      901 acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattgagggt ggtgaatgtg
      961 ctccgactca tgatcatgaa gcttttacaa agggagaccc tggacctgca ccgcgaggtc
     1021 gaggccctga tgcccgtgat gagtgagacg ctgacccggc ggggctatct ggaggtgctg
     1081 cggcgcctgc aagcggtggt cgcgccgctg gaagcacaac tcgacgccct gccgctgccg
     1141 gacgagctgg cgtggcccga gcgccgcaag gcccatctgc tcgctctgga ccttgccgta
     1201 atgggggaag ggccgtcaaa tcagcaggcg ccactgtctg tgcccgagct gaccactctg
     1261 gcgcaggcct tcggagcgct ttacgtgctt gaaggcgcga cgctaggcgg acagctcatc
     1321 agccgccatc tcaggcgcac gctggggcta agccctgagc agggcagcgc ctatttctcc
     1381 ggctatggcc cccagaccgg cccgcgctgg cgctcgttcg gggaagtgct ggaagccagc
     1441 gtgccggcag aagacgcggc agaggtcgtg gcgggagcgc ggcagacctt cggggccttc
     1501 cgccgcgcct tgcaggggct gtcggaagct gaagcggtcc aagtgcctga ggtcgcccac
     1561 gcatgactgc aacccagacc gttgatctga caaattgtga tcgtgaaccg attcatattc
     1621 cgggtagcat tcagccgcat ggttgtctgc tggcatgtga tgcagaaatg gcagttgttc
     1681 tgcgtcatag cgcaaatgcc ggtgaaatgc tgggtgttaa aggtgcagtt aatggtgaac
     1741 aggttcatgt tctgctgggt gatgatgcaa cccatgatct gcgtaatgca ctggcaaccg
     1801 caccggaagc aagccgtccg gcactgctgc cgaatctgcg tctgagctgt ggtcaggttt
     1861 ttaatgttag cgttcatcgt cagcgtgcca ccgttattat tgaactggaa ccggcaaaag
     1921 gtgaacgtga tccgctgcag ctggcacgtg aaatgattgg tcgtattaaa gacatcgacc
     1981 acatcgataa actgattcgt accagcgcac gtctggttcg tgcagttctg ggttatgatc
     2041 gtgttatgat ttatcgcttt gataatgatg gtagcggtaa agttgttagc gaagcaaaaa
     2101 gcatggaact ggaaagtttt ctgggtcagt attttccggc aagcgatatt ccgcagcagg
     2161 cacgtaaact gtatatgcag aatagcattc gcgttattag tgatgcaagc ggtcataaag
     2221 ttgccattga accggaactg gatgccagcg gtgaacctct ggatctgagc tttgcacatc
     2281 tgcgtagcgt tagcccgatt catctggaat atctgcgcaa tatgggtgtt ggtgcaagca
     2341 tgagcattag cattctgctg aatggcgaac tgtggggtct gattgcatgt catcactatc
     2401 gtgcaaaaac cctgagcatg gcacagcgtg ttgcagcaga actgtttggt gaatttttca
     2461 gcctgaccct gctggccctg aaacagaaac ataaactgga tctggccacc ggcacccgtc
     2521 agagcctgga tcgttttctg cacctggcaa gccatcatga aaatctggaa gcactgtttg
     2581 gcgagtgtct gccggaattt tcaagcctgg ttccgtgtga tggtgcaggt ctgtggatga
     2641 atggcacctg gaccgccatt ggcacctgtc cgcctccggc agaaattgaa ccgctgattc
     2701 gttttgttaa tagcgttgca gatggtcaga cctgggccag ccatgcactg agccagtttc
     2761 tgcctcgtgc agaaagctat tatgaaaccg ttagcggtgc aatggttgtt ccgctgagcg
     2821 aaattccgcg tgattatctg ctgttttttc gtaaagaacg cctgcagacc ctgaattggg
     2881 caggtaatcc ggataaaagc tatgaaattg gtccgctggg cgatcgtctg acaccgcgta
     2941 aaagttttgc aatttggaaa gaagcagtga ccaaacagag cgatcattgg agcgaaaatg
     3001 atcgcgaagt tgccgaagca attcgtgccg ttaccgttaa agtggttctg cgccataatg
     3061 aactgatgca agaggagcgt aaaaccgagc atcagcagac acaagcacgt ctccaggaac
     3121 tgcaatccga gctcgtccac gtctccaggc tgagcgccat gggcgaaatg gcgtccgcgc
     3181 tcgcgcacga gctcaaccag ccgctggcgg cgatcagcaa ctacatgaag ggctcgcggc
     3241 ggctgcttgc cggcagcagt gatccgaaca caccgaaggt cgaaagcgcc ctggaccgcg
     3301 ccgccgagca ggcgctgcgc gccggccaga tcatccggcg cctgcgcgac ttcgttgccc
     3361 gcggcgaatc ggagaagcgg gtcgagagtc tctccaagct gatcgaggag gccggcgcgc
     3421 tcgggcttgc cggcgcgcgc gagcagaacg tgcagctccg cttcagtctc gatccgggcg
     3481 ccgatctcgt tctcgccgac cgggtgcaga tccagcaggt cctggtcaac ctgttccgca
     3541 acgcgctgga agcgatggct cagtcgcagc gacgcgagct cgtcgtcacc aacacccccg
     3601 ccgccgacga catgatcgag gtcgaagtgt ccgacaccgg cagcggtttc caggacgacg
     3661 tcattccgaa cctgtttcag actttcttca ccaccaagga caccggcatg ggcgtgggac
     3721 tgtccatcag ccgctcgatc atcgaagctc acggcgggcg catgtgggcc gagagcaacg
     3781 catcgggcgg ggcgaccttc cgcttcaccc tcccggcagc cgacgagatg ataggaggtc
     3841 tagcatgacg accaagggac atatctacgt catcgacgac gacgcggcga tgcgggattc
     3901 gctgaatttc ctgctggatt ctgccggctt cggcgtcacg ctgtttgacg acgcgcaagc
     3961 ctttctcgac gccctgccgg gtctctcctt cggctgcgtc gtctccgacg tgcgcatgcc
     4021 gggccttgac ggcatcgagc tgttgaagcg gatgaaggcg cagcaaagcc cctttccgat
     4081 cctcatcatg accggtcacg gcgacgtgcc gctcgcggtc gaggcgatga agttaggggc
     4141 ggtcgacttt ctggaaaagc ctttcgagga cgaccgcctc accgccatga tcgaatcggc
     4201 gatccgccag gccgagccgg ccgccaagag cgaggccgtc gcgcaggata tcgccgcccg
     4261 cgtcgcctcg ttgagcccca gggagcgcca ggtcatggaa gggctgatcg ccggcctttc
     4321 caacaagctg atcgcccgcg agtacgacat cagcccgcgc accatcgagg tgtatcgggc
     4381 caacgtcatg accaagatgc aggccaacag cctttcggag ctggttcgcc tcgcgatgcg
     4441 cgccggcatg ctcaacgatt gacaattgat gtaagttagc tcactcatta ggcaccggga
     4501 tctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     4561 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     4621 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     4681 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     4741 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccccacgg
     4801 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact
     4861 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg
     4921 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa
     4981 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg
     5041 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt
     5101 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac
     5161 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc
     5221 attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa
     5281 accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc
     5341 aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat
     5401 gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     5461 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     5521 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     5581 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     5641 accattgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     5701 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5761 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5821 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5881 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5941 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6001 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6061 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6121 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6181 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6241 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6301 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     6361 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6421 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6481 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6541 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     6601 ttcaacggga aacgtcttgc tctaggccgc gattaaattc caacatggat gctgatttat
     6661 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt
     6721 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     6781 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     6841 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggga
     6901 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     6961 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     7021 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     7081 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     7141 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     7201 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     7261 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     7321 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     7381 atttgatgct cgatgagttt ttctaagaat taattcatga gcggatacat atttgaatgt
     7441 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     7501 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     7561 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     7621 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     7681 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     7741 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     7801 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     7861 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     7921 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gcca
//
