LOCUS       sequence-472448-        9752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression of KmTor by integrating ZZ-KmTOR into the K. 
            marxianus genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Baretic D, Berndt A, Ohashi Y, Johnson CM, Williams RL
  TITLE     Tor forms a dimer through an N-terminal helical solenoid with a 
            complex topology.
  JOURNAL   Nat Commun. 2016 Apr 13;7:11016. doi: 10.1038/ncomms11016.
  PUBMED    27072897
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-13"; volume: "7:11016. doi"; pages: " 
            10.1038/ncomms11016"
COMMENT     Sequence Label: pYO503
FEATURES             Location/Qualifiers
     source          1..9752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            complement(700..1819)
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     terminator      complement(700..897)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(903..1475)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(1476..1819)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    1907..2024
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     2205..2228
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             2361..2381
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2397..2570
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             2571..2744
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             2754..2774
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2805..2825
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2847..2867
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(7564..7584)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7564..7582)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7603..7619)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7603..7619)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7616..7638)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7627..7643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7651..7657,7658..7675,7676..7681))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7696..7717
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7834..7851)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8005..8593)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8085..8104)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8764..9555,9556..9624))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9387..9406
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9625..9729)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgt
      661 tttcgacact ggatggcggc gttagtatcg aatcgacagc agtatagcga ccagcattca
      721 catacgattg acgcatgata ttactttctg cgcacttaac ttcgcatctg ggcagatgat
      781 gtcgaggcga aaaaaaatat aaatcacgct aacatttgat taaaatagaa caactacaat
      841 ataaaaaaac tatacaaatg acaagttctt gaaaacaaga atctttttat tgtcagtact
      901 gattaggggc agggcatgct catgtagagc gcctgctcgc cgtccgaggc ggtgccgtcg
      961 tacagggcgg tgtccaggcc gcagagggtg aaccccatcc gccggtacgc gtggatcgcc
     1021 ggtgcgttga cgttggtgac ctccagccag aggtgcccgg cgccccgctc gcgggcgaac
     1081 tccgtcgcga gccccatcaa cgcgcgcccg accccgtgcc cccggtgctc cggggcgacc
     1141 tcgatgtcct cgacggtcag ccggcggttc cagccggagt acgagacgac cacgaagccc
     1201 gccaggtcgc cgtcgtcccc gtacgcgacg aacgtccggg agtccgggtc gccgtcctcc
     1261 ccgtcgtccg attcgtcgtc cgattcgtcg tcggggaaca ccttggtcag gggcgggtcc
     1321 accggcacct cccgcagggt gaagccgtcc ccggtggcgg tgacgcggaa gacggtgtcg
     1381 gtggtgaagg acccatccag tgcctcgatg gcctcggcgt cccccgggac actggtgcgg
     1441 taccggtaag ccgtgtcgtc aagagtggta cccatggttg tttatgttcg gatgtgatgt
     1501 gagaactgta tcctagcaag attttaaaag gaagtatatg aaagaagaac ctcagtggca
     1561 aatcctaacc ttttatattt ctctacaggg gcgcggcgtg gggacaattc aacgcgtctg
     1621 tgaggggagc gtttccctgc tcgcaggtct gcagcgagga gccgtaattt ttgcttcgcg
     1681 ccgtgcggcc atcaaaatgt atggatgcaa atgattatac atggggatgt atgggctaaa
     1741 tgtacgggcg acagtcacat catgcccctg agctgcgcac gtcaagactg tcaaggaggg
     1801 tattctgggc ctccatgtcg ctggccgggt gacccggcgg ggacgaggca agctaaacag
     1861 atctgcggcc gcggatcttt gcaaagatcc aaaaaaagca tgcgtacgga ttagaagccg
     1921 ccgagcgggt gacagccctc cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg
     1981 tcgcgttcct gaaacgcaga tgtgcctcgc gccgcactgc tccgaacaat aaagattcta
     2041 caatactagc ttttatggtt atgaagagga aaattggcag taacctggcc ccacaaacct
     2101 caaatgaacg aatcaaatta acaaccagat atctcgactg aaaaaaaagg tttaaaccag
     2161 ttccctgaaa ttattcccct acttgactaa taagtatata aagacggtag gtattgattg
     2221 taattctgta aatctatttc ttaaacttct tgaattctac ttttatagtt agtctttttt
     2281 ttagttttaa aacaccaaga acttagtttc gaataaacac acataaataa accatgatgg
     2341 gagcaggggc gggtgctagc gagaacctgt acttccaggg cgcccacgat gaagccgtag
     2401 acaacaaatt caacaaagaa caacaaaacg cgttctatga gatcttacat ttacctaact
     2461 taaacgaaga acaacgaaac gccttcatcc aaagtttaaa agatgaccca agccaaagcg
     2521 ctaacctttt agcagaagct aaaaagctaa atgatgctca ggcgccgaaa gtagacaaca
     2581 aattcaacaa agaacaacaa aacgcgttct atgagatctt acatttacct aacttaaacg
     2641 aagaacaacg aaacgccttc atccaaagtt taaaagatga cccaagccaa agcgctaacc
     2701 ttttagcaga agctaaaaag ctaaatgatg ctcaggcgcc gaaagtagac gcggaaaacc
     2761 tgtatttcca gggaggcgga tcttctagac caactactgc ctctgaaaat ttgtactttc
     2821 aaggcgagtt gaagaccact gcttctgaaa acttgtactt ccaaggtgag ttactcgagg
     2881 gtgaattgaa gactgctgct ttggctcaac acgacgaagc catgggcgga tcccttagaa
     2941 gaaaggggga gtctgtgaag aaaaggttgg tgtcaggtac agcagcctca ttgcaagaac
     3001 tctccttaca gccttcttct aatggtattg catcggctgg aaagagcgaa gatggagagg
     3061 ctggtgatgg aatgtctgga gaatttgggg cagcattgac tgattcgaat gtggagaaca
     3121 atgttaaggc atttgatttg atctttacga agcttaaaag tacgaatcaa ctggaacgag
     3181 tggcagcgtc atacgagttg aaatcgtgtc tgatttcgct agctcgagag gtttctacag
     3241 agtacttcca gcgtttcagc aatgatataa ataataaggt tttcgagttg atccatggga
     3301 ccgattctaa tgagaagctt ggtggtgtgc tagctgttga tacgttgatt gatttctatt
     3361 cacatactga tgaattgccc aatcagacgt cgagattagc gaattacttg cgtgtgttaa
     3421 taccgtccaa cgacatcgag gtgatgagag ctgcggcgac gacgttgggg aagttagcgg
     3481 tgcccggtgg gacattaacg tcagagtttg ttgagttcga ggtgaaaact tgcattgaat
     3541 ggttgacgac ttctccggag agttcctctt cgaattccaa gcaagagtac agaaagcatg
     3601 catctttgtt gattatcagt tcgatcgcgc acaactcgcc atatttacta tacccgttcg
     3661 tgaactcgat cttagataac atatggaggg ctctaagaga cacgaagcta gttatcagaa
     3721 ccgatgcagc ggtcacgcta gggaaatgtt tgacaattat caacgatcgt gactcttctt
     3781 tgactaaaaa atgggtacaa agacttttca atggctgcat atacggattg caactaaata
     3841 caacggtgtc tatacacggt actctattgg tatacagaca acttgtgtcc ctagaagggg
     3901 gatacctgaa ggacaaatat gaagaaattt acgagacaac aatgaagcat agagacaata
     3961 agaatattat tatcagaaag gaaatatatg ctatcattcc tgttttggca gcatttgatc
     4021 caaaactctt cactcaaaaa tatttggatg caaccatgat acattaccta acactattga
     4081 agaacatgac ttctcaccaa attgcgctat cggataaagg ccctatatta gtctccatcg
     4141 gtgatattgc atatcatgtg ggttccgaaa ttggcccata ccttgatgcc attgtagaga
     4201 atttaaggga tggtctagaa acaaagttca aaactaggaa agaattcgaa aaggacatat
     4261 tttattgtgt tagcaaacta gcatgtgctg ttaggccatt gttagccaaa tatttaaaca
     4321 gaggattgct tgacgctcta ttggcatgtc ccttgagcga tcatatgcaa gaaactcttc
     4381 tggttatttg cgaaaaaatc ccttcgatgg aagtcacagt taatgagaag cttttaaata
     4441 tcatttgttt ggttctttca ggggagaagt tcagacctcc aggatcacca acgccaatga
     4501 aatcgctatc gagcgaaact gcaagggctt atagagacca atctttactc agaaagacag
     4561 gtgaaaataa taatgatact ctcgacgcaa tgattctcag aaaagcttta agaatgcttt
     4621 cagatattaa accaaaatat tctttgactg aatttattag gagagttata atatcctaca
     4681 tggaacatga taatatgcaa gtcagaaaac tagctgctct gacttcttgt gacttgttcg
     4741 taaaggattc aatctgcaaa caaacctcat tatatgcatt aaatgtggtt tcagaggttt
     4801 tgagtaagct attaacggtc gcaattacag atccagtggc ggaaatccgt ctagaaatac
     4861 taaaacattt agacaggaac tttgatccac aactttctca accagataat acaaaacttt
     4921 tatttatggc gctgaatgac gaagtttttg ccatccaaat ggaagcaatg aaaatctgtg
     4981 gtagacttgc cttggtgaac ccagcatatg tcattccatc tttaaggaaa actttaattc
     5041 aactattgac tgagctaaaa cactctaaaa tgacaagaaa aaaggaagaa tgtgcatcat
     5101 tgctctgtac tttgatcagt tccagtagcg acgttaccaa accttacctt gagcctgtga
     5161 ttgagatttt gcttccaaag tctcaagatc catcttctgc tgtagcatca acagcgttaa
     5221 aagctttagg agaaatatca gtcgttggtg gtgaagatat gctacctttc ttggatgaat
     5281 taatgccatt aatcatcgat acattccaag atcaatccaa ttctttcaag agaggggctg
     5341 cactaaaggc tttaggacaa ctagctgcct cttcaggata cgttatcgat cctttattag
     5401 actaccctga attactcggt gtgcttatca atattttgaa atcagaaagt tcacagaata
     5461 ttcgcagaga aactgtcaga ttgatcggta ttctcggggc attagatcca tacaaacata
     5521 gagaagtgga aagaacatct ataacaaata ttacttctga gcaaaatgcg cctcctattg
     5581 atgtcgcatt actaatgcaa ggtatgtcac catctaacga agaatactac ccaacagttg
     5641 tcatcggcgt tctaatgaaa atattgaaag atccttcctt atccatacac cattctactg
     5701 tcattcaagc aataatgcat attttccaaa caatgggtct tcgttgtgtc attttcttaa
     5761 agcagattat tcctggtttt atacttgtga tgcatacttg tccaccatcc cttcttgaac
     5821 tttatttcca acaactcagt gtattaatat ccattgtaaa gcaacatatc agaccacatg
     5881 ttaccgaaat tgttgatgtt atttcagaat tcttccctgt attgaagttg cagctaacga
     5941 tcatatctgt tattgaatcg ttatcgaggg ctttagaagg tgagtttaat cctcatttac
     6001 ctactattct ttctttgttt ttagatgttt tggagaaaga tcaatcaaac aagaaagttg
     6061 tatctgttag aattttgaaa tctttggtgg tgttcggtcc tcatctagaa gaatatgtcc
     6121 atcttgttct acctaccatt attaaactat ctgaattcag ctcagggaat ttgaaaaaag
     6181 ctgcaattat cactattggt aggttatcca agaatgttaa tgcattggaa atgtcttcaa
     6241 gaatcgttca agctttggtc agagtcttaa atacttcgga aacggaatac gtgaaagcaa
     6301 caatgaacac cttaagttta ctacttttgc aattaaacat tgatttcact gtattcatac
     6361 cagtaatcaa taaaacgttg gtaaaacaga atatccaaca taccatatat gatcgcttag
     6421 ttgcaaagct tctaaacaat gaacctttac caacaaaaat tattattgac aaggactttg
     6481 atttacctaa caaagaaatg gatgatgtca aggttgcatc gaagaaactc ccggtcaatc
     6541 aaatggtttt gaaaaatgct tgggattgta gccaacagag aacaaaagaa gattggcaag
     6601 agtggattag aagactttcc atatctttac tcaaggaatc atcatctcat gcattgcgtg
     6661 catgctctgg attggcaggt atatattacc cattggctag agaacttttc aacgcttcgt
     6721 ttgcaagttg ttggggtgaa ctttacacac agtaccaaga ggatttgata caatcacttt
     6781 gcgcagcttt atcttcacca aacaacccac ctgaaatcca tcagactcta ttaaatttag
     6841 ttgagttcat ggaacacgat gacaaacccc taccaatatc gatttcgact ttaggcgaat
     6901 acgcccaacg ttgccatgcc tatgcaaagg ctcttcatta caaggaatta gagtttatcg
     6961 aagaaccttc gacatctacg attgagtcat taataacaat taataatcaa ttacatcaaa
     7021 cggatgcggc aatcggtata ttgaagcacg ctcaacaaca ccatgatttg caattgaaag
     7081 aaacttggta cgagaaactt caaagatggg atgatgcatt gaatgcatac aacaaacgtg
     7141 aagctgctgg ggaagattca gtagaggtga ccattggtaa aatgagatct ctacatgctt
     7201 tgggtgaatg ggatcaactg tctgaactcg ccgctgataa gtgggcttct tctcaactag
     7261 aagttaagag agctatcgca ccacttgccg ctggtgcagc atggggctta gcacaatggg
     7321 atagagtaga gcaatatatt gaagtgatga agccacaatc tccagacaaa gctttctttg
     7381 acgctattct atgtctccat cgtaacaatt ttgaaaaggc ggcggaacac attttctctg
     7441 ccagagatct tctagttact gaaatgtctg cattggtaaa tgagagttat aatagagctt
     7501 acggtgttgt ggtaagaact caaatggttg ctgagttggg ggcccggtac ccagcttttg
     7561 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     7621 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7681 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7741 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7801 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7861 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7921 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7981 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8041 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8101 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8161 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8221 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8281 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8341 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8401 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8461 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8521 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8581 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8641 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8701 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8761 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8821 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8881 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8941 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9001 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9061 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9121 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9181 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9241 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9301 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9361 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9421 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9481 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9541 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9601 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9661 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9721 ggttccgcgc acatttcccc gaaaagtgcc ac
//
