LOCUS       sequence-47245-e         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HPV 8E6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Spangle J, Ghosh-Choudhury N, Munger K
  TITLE     Activation of cap dependent translation by mucosal Human 
            Papillomavirus E6 proteins is dependent on the integrity of the 
            LXXLL binding motif.
  JOURNAL   J Virol. 2012 May 2.
  PUBMED    22553330
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2012 May 2."
COMMENT     Sequence Label: pNCMV 8E6
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             40..66
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atggactaca aggatgacga tgacaagctc gatggaggat acccatacga tgttccagat
       61 tacgctgata tcggatccga cgggcaggac aaggcttcat atttagacac taataaggac
      121 gagctaccct ctactattaa agagttagct gcggctttag gtattccatt gcaggactgt
      181 ttagtaccgt gcaacttttg tggtaacttt ttggatttct tagaactgtg tgagtttgac
      241 aaaaagagac tgtgcctaat ttggaaaaat tacgttgtta ctgcgtgttg tcgttgttgt
      301 tgtgtagcaa ccgcaacgtt tgaatttaat gaatattatg agcaaactgt gctaggcaga
      361 gatattgaat tagctacagg acgttcaatt tttgagatag acgttaggtg tcaaaactgc
      421 ttgtcatttt tggatatcat agagaaatta gattgctgtg ggagaggccg tccctttcat
      481 aaagttagag gaggctggaa aggagtttgc aggctttgta agcatttgta tcatgattgg
      541 taaagatccg tcgaggaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct
      601 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
//
