LOCUS       sequence-472451-        3179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-229E-nsp1
FEATURES             Location/Qualifiers
     source          1..3179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     597..613
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     597..613
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     618..637
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        618..636
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    654..753
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1143..1242)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1258..1275)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1258..1274)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1267..1289)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      1338..1424
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1516..1543
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1702..1719)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1873..2461)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1953..1972)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2554..3179,1..166))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg
       61 agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta
      121 cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcataatg tttaactttg
      181 ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
      241 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac
      301 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg
      361 ttctggacca gttgcgtgag cgcatacgct acttgcatta cagcttacga accgaacagg
      421 cttatgtcca ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct
      481 tgggtagcag cgaagtcgag gcatttctgt cctggctggt ctagaggagg catgatgata
      541 tatttttatc ttgtgcaatg taacatcaga gattttgaga cacgggccag agctgccagg
      601 aaacagctat gaccatgtaa tacgactcac tataggggat atcagctgga tggcaaataa
      661 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caattatttt
      721 ttataatgcc aactttgtac aagaaagctg ggcccatgag gccgcgatcg cgtcttaatt
      781 aagaccacca cctctcttac cgaagacaac atcaaattcc tccaaaaggt aatttgaatt
      841 ggaaaaaacc aaccacccat gaagcataaa aggacggtca gaaaatttca ttatgttgca
      901 aaacaacgtc tgattgccat gcagacccat aacatatgta tcgtctgcaa tgccaacgat
      961 gcaatcctgc aaatctaatg aaacaaatcg gcatgcccta aaaccattgc tagcggcctc
     1021 gctataacgg cggacggctt gcgcaatagt agaacagcca tttgcagaaa tttcagaatc
     1081 acttgctacg gcaagtgtca cacggttgca ggcggatccc atggatcctg gccttgacgg
     1141 cccttctgct tttttgtaca aagttggcat tataaaaaag cattgctcat caatttgttg
     1201 caacgaacag gtcactatca gtcaaaataa aatcattatt tggggcccga gcttaagact
     1261 ggccgtcgtt ttacaacgtc gtgactggga aaacatccat gctagcgtta acgcgagagt
     1321 agggaactgc caggcatcaa ataaaacgaa aggctcagtc ggaagactgg gcctttcgtt
     1381 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
     1441 tgaacgttgt gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
     1501 ggcatcaaac taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc
     1561 ttcctggcta gcggtacgcg tattaattgc gttgcgctca ctgcccgctt tccagtcggg
     1621 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     1681 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1741 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1801 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1861 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1921 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1981 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2041 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2101 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2161 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2221 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2281 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2341 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2401 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2461 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgacg cgtaactcac
     2521 gttaagggat tttggtcatg ggtggctcga gggttatttg ccgactacct tggtgatctc
     2581 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     2641 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     2701 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     2761 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     2821 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     2881 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     2941 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     3001 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     3061 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     3121 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagcctta
//
