LOCUS       sequence-472453-        3098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-229E-nsp7
FEATURES             Location/Qualifiers
     source          1..3098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(15..603)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(95..114)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(696..1487)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     1918..1934
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1918..1934
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1939..1958
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1939..1957
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1975..2074
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2383..2482)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2498..2515)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2498..2514)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2507..2529)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2578..2664
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2756..2783
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2942..2959)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
       61 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      121 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      181 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      241 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      301 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      361 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      421 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
      481 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
      541 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
      601 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga cgcgtaactc
      661 acgttaaggg attttggtca tgggtggctc gagggttatt tgccgactac cttggtgatc
      721 tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct
      781 tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc
      841 aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg
      901 ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc
      961 ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc
     1021 ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt
     1081 gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg
     1141 tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg
     1201 atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg
     1261 gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt
     1321 acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg
     1381 gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact
     1441 acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcataat gtttaacttt
     1501 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga
     1561 cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac cccaaaaaaa
     1621 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     1681 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagcttacg aaccgaacag
     1741 gcttatgtcc actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc
     1801 ttgggtagca gcgaagtcga ggcatttctg tcctggctgg tctagaggag gcatgatgat
     1861 atatttttat cttgtgcaat gtaacatcag agattttgag acacgggcca gagctgccag
     1921 gaaacagcta tgaccatgta atacgactca ctatagggga tatcagctgg atggcaaata
     1981 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaattattt
     2041 tttataatgc caactttgta caagaaagct gggcccatga ggccgcgatc gcgtcttaat
     2101 taagttgcaa aatggagtcg ttctcaaaat aagaatcgac aagatcacca agaccaaaat
     2161 cactatgctt agacaagaaa aaggccaaca acgccagcaa taactcttga gcagtttcag
     2221 ggtcgtcaca caagtttatt ttattgtgca tttcaacaca atatgcccac tcctttgaat
     2281 tagaagctat gttcatgtta gacaaaatgc ccattagaac gacgtttgtg cacttaagat
     2341 cagtcaattt agaggatccc atggatcctg gccttgacgg cccttctgct tttttgtaca
     2401 aagttggcat tataaaaaag cattgctcat caatttgttg caacgaacag gtcactatca
     2461 gtcaaaataa aatcattatt tggggcccga gcttaagact ggccgtcgtt ttacaacgtc
     2521 gtgactggga aaacatccat gctagcgtta acgcgagagt agggaactgc caggcatcaa
     2581 ataaaacgaa aggctcagtc ggaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2641 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgt gaagcaacgg
     2701 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaac taagcagaag
     2761 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttcctggcta gcggtacgcg
     2821 tattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     2881 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2941 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     3001 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3061 caaaaggcca gcaaaaggcc aggaaccgta aaaaggcc
//
