LOCUS       sequence-472464-       10375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses A. thaliana Cpn60-alpha Cpn60-beta Cpn20 Raf2 RbcX Bsd2 S.
            tuberosum Raf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10375)
  AUTHORS   Archer J, Kathpalia M, Lee BU, Li S, Wang T
  TITLE     Effects of chaperone selectivity on the assembly of plant Rubisco 
            orthologs in E. coli.
  JOURNAL   J Exp Bot. 2025 Mar 31:eraf140. doi: 10.1093/jxb/eraf140.
  PUBMED    40163664
REFERENCE   2  (bases 1 to 10375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Mar 31:eraf140. doi: 10.1093/jxb/eraf140."
COMMENT     Sequence Label: pTW334g
FEATURES             Location/Qualifiers
     source          1..10375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             86..877
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        join(1194..1199,1200..1215,1216..1222)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1230..1246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4510..4532
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             5799..5807
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             6225..6247
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    7969..7985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7993..7999,8000..8015,8016..8021))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        8264..8341
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8342..9424
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8361..8380)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      9581..10319
                     /direction=RIGHT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     promoter        join(10369..10375,1..85)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
       61 tcaataatat tgaaaaagga agagtatgag ggaagcggtg atcgccgaag tatcgactca
      121 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      181 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
      241 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      301 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      361 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      421 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      481 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      541 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
      601 atggaactcg ccgcccgact gggctggaga tgagcgaaat gtagtgctta cgttgtcccg
      661 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
      721 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
      781 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
      841 cgtgaaaggc gagatcacca aggtagtcgg caaataaacg ccatgggcat taaaaaatgc
      901 cctcttgggt tatcaagagg gtcattatat ttgctgagga gtgccgttaa tgcgcaacgc
      961 aattaatgta agttagctca ctcattaggc acaattctca tgtttgacag cttatcatcg
     1021 actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg
     1081 tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa
     1141 tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa
     1201 ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     1261 cagccagtcc gtttaggtgt tttcacgagc acttcaccaa caacgcgtac aactaggaga
     1321 tataccatgg gtatgcaaca gttataccag ccttttcgtc cgccaccgtc gccgcttccg
     1381 cccaaattca ggcacttgga taccaactcc aagttggaag tgctggccaa ccgactgggc
     1441 ctgtggtatg agtatgcccc tttaattccg tcgctgacct cagaaggttt tactccgagt
     1501 acactagaag aaattaccgg tattaccggc gtcgagcaaa atcgcttggt ggtcgccgcc
     1561 caagtccgcg ataccctcgt ggaatgtggc ctggatgaag aaacgctcag cttcttcgag
     1621 tcaggcggcg cggaactcct gtatgagatc cgcctgttaa gtgttaagca gcgggcggat
     1681 gcggcgcgct ttatggtgcg caatggattt gacatgaaac aggcgcagga cctggcacgt
     1741 gccatgaaag actttccgcg tcggcgtatt gattatggct gggacaaatt tacgggtgac
     1801 tcccctggtg attgtcttgc attttggttc ttccgtctgg cacaagagca tgcggccgcg
     1861 gcagccgaag agagccgtgt tgaagcaatg gaaaaagcgc tggaggtagt tgaaaccgaa
     1921 tcggctcgca atgttctggt tgaggttctg gaagggaaag gtgtcgataa ggatagcgtg
     1981 atcgacgagc aggtaaaagt gcctttagtt cgcatgaaac taggggaagt cgcggagtct
     2041 actaaggtag tagtgctgcc agtatgcaaa gcggaaaaac gtgaatttga agtcgaagca
     2101 gccccgtggg aatgcggagg cgttggtgat ttcggcgtgg ttgaggctga aaaagactgg
     2161 cgccgctggg tggttcttcc gggctggcag ccaattgccg gccttgaacg tggtggtgtc
     2221 gcggtgagct tcaaaagcgg aaaactgctg ccctggaaag aaaagcgtaa gtacaaagaa
     2281 gaaccggtgt tggtggtggc tgaccgcggt agaaaggaag tggtcgttga tgatggcttt
     2341 tacttagtac tgtcaggcgg caacgggtct ggcgatgagg gcctcatggt ggaaaaaggg
     2401 ttgacgctga aagaaatggg tgtggaaaaa agcttaggaa tggtgcttct ggtggtacgc
     2461 cccccgcgat gggaagatga ggagcagctg atcgatgaag attaaattac aaaaattagg
     2521 agatatacat atgatgtcta atctggcgca ggattttctt ggtgacttcg gagctcgtga
     2581 cccttacccg gaggagatag cgagtcaatt cggagataaa gtgttgggat gccaaagcac
     2641 tgagcacaag attttgatac caaacgcatc tgttttgtct ctctcccagc ttcagtgttc
     2701 ccctgtttcg tcttcacagc ctcctttgtc cggcgatgat gccagaactc tcctccacaa
     2761 ggttttggga tggagtatag tggataatga agcgggtggt ctgaaaataa ggtgtatgtg
     2821 gaaagtgagg gattttgggt gcggtgttga actcataaac aggatccata aggttgctga
     2881 agcttctggt cattaccctt ctcttcactt ggaaagtcct acccaagttc gagctgaact
     2941 atttacctct tctatcggag ggttgagcat gaatgatttc ataatggcgg ctaaaataga
     3001 tgatatcaag acttctgatc tttcccctag gaaaagagct tgggcgtgac ggctgaaatt
     3061 aatgaggaga tatacatatg gtatcgagaa ttgcgaatcg aaagagcaaa aagctcttga
     3121 ttgtgaatga ggatgttgct ggtaattacg acgatacctt cggtgatgta caaaagcaaa
     3181 ttgttaatta ttttacatac aaagctgtga ggacggttct tcatcagctg tacgaaatga
     3241 atcctcctca atacacttgg ttctataatc atatcataac aaacagaccg acggatggca
     3301 aacgtttcct ccgtgccctc ggcaaggaga gtcaggagct tgcagaaaga gtgatgatca
     3361 cgcgtcttca cttgtatggc aaatggatca agaaatgcga ccatgggaag atataccagg
     3421 aaatatcgga tgaaaacttg gcgttgatgc gtgaacgcct gatggagaca gtgatatggc
     3481 cttccgatga cacaaactca aggtaattca gctaagacac tgcacaggag atatacatat
     3541 gatggccgca aacaataatc ctcagggcac taaaccaaac agtttagttt gtgcaaattg
     3601 tgaaggggaa ggttgtgtgg catgttctca atgcaaagga ggtggtgtga atttgattga
     3661 tcatttcaat ggtcagttta aagctggtgc actctgttgg ctttgcagag gtaaaaagga
     3721 ggtgttgtgt ggggactgca atggagctgg tttcattggt gggttcttga gcaccttcga
     3781 tgagtagagt cagtagggcc ctaaaaaaaa accccgcccc tgacagggca gggttttttt
     3841 tggcgaaaaa accccgctga agcggggttt tttgcgtaca ttggctccgt ctaagaaagt
     3901 atagccatca catctgaagc tctgagggca atgtagttgg aaccatcttt gcccttgaag
     3961 tcgttaccag catacttgga gtaaagtact gtgcttccgg ttgatactgg tagaggcgta
     4021 attttacctt cctcgtctag ggaacccggt ccaactgcta tcacggtgcc aatagaaggc
     4081 ttctctttgg tagtctcggt taacaacaac cctccagctg ttttctcctc cgcctcagca
     4141 accttaataa agactcggtc attcaaaggt ttgagatctt tgatgtcctc tgtctcaaga
     4201 atgccaacaa tatcatcttc cttgaggata agatgcttca catcattgaa ctccacctca
     4261 gttcctgcgt atttggagta gataatttgt gctccagtag ggacagtgat atcaattttg
     4321 ttcttcccaa tagttcttcc ttcacccaca gcaacgactt cacctccttg aggttttgat
     4381 tgagcagtgg atggaagtaa gataccacct aaagtcttct cctctgcctc cttgatcttc
     4441 accaaaactc gatctcccaa tggcttaatt gaagtatact taggggcaac aacagaggcc
     4501 atatgtatat ctccttctta aagttaaaca aaattatttc tagagggcaa ttgttatccg
     4561 gtcgacgtcg accttagtat ccgtaacctg agttgtccat tgggttgccg acgggaactg
     4621 gttcaggttc cttgatctca acaacaacgc agtccgacat caagaaagtc tttgcaaccg
     4681 aagctgcgtg ttccaagcaa catctcacaa cctttgttgg atcgatgatt cctgcagcca
     4741 tcaaatcctc gtacttgccg gttgcagcat tgtaaccgaa tttcacgtta tcgttggaaa
     4801 gcaccttctc gctaactaca cttccattaa caccagcatt cttggcgatt agttttaggg
     4861 ggtaacttag tgctctttta actatatccg ctccaacctt ttcttcatca ttatcaagcg
     4921 tggctttaat ggcatcaacc ttggaagcaa gacgaagcag agtgcaacca ccaccaacaa
     4981 caataccttc ctcgacagcg gcctttgttg cattaagagc atcttcaact ctcaacttct
     5041 tctctttgag ttctgtctca gtttgtgctc caacctgaat cacagcaact ccaccagaga
     5101 gctttgcaat tctctcattg agtttctcct tctcgtaatc ttgctccgct tgctcaataa
     5161 gatttttaat ctgtgtaaca cgctttttca ctgcatcctg tgtgcttcca tcacccacta
     5221 tggtcgaggt ttcctttgtg aggacaacct ttgaagcatt accaagaacc tcttttccag
     5281 ctttgtcaag tgaaagacca acttcctcac ggatcacagt tgctccagtt agaatggcaa
     5341 tatcatcaag gtattggctc ttgcgctctc caaaccctgg agctctgaga gctgctatct
     5401 tcagtgtacc tctaagcttg tttacaacaa gagtcgctaa agcttcttgt tcaatgtctt
     5461 cagcaataat taatattggg tatccgcctc taattgcatc ctccaaaaca ccaacaagat
     5521 cccttgcatt ggtaattttc ttgtcaacga gaagtaactt gcaattgtcg aactcgacgg
     5581 acattttctc gctgtctgtc acaaagtaag gagagatata accacggtca aattgcattc
     5641 cttctacgac gtagaggttg ttctcggcac ttttaccctc ctcaagggta accacacctt
     5701 tcctgcccac tttgctcatt gcttcagcaa tcatatttcc aatttcgtcg ttgttacccg
     5761 cactaacagc tgcaacatct gcaagttcac tgtcttcaac ctccttagac attttcttga
     5821 gctcggtaac caaagccttt gctgtcttct caatacctct ggtgatcaaa acagggtttg
     5881 caccagcagc taccaccttg acaccctcag cgataaaacc ttgtgcaaga acaacagatg
     5941 ttgttgtacc gtcaccagcc aagtcattgg ttttggcagc cgcttgcctc acaagcttag
     6001 cgccaatgtt ctcaaccggg tcttccaact caacctctct tgcaacagtc acaccatcat
     6061 taacaattcg tggtgatcca tacttgctct caagaacaac atttcgtcct ttgggtccaa
     6121 gtgtaacgcc aacaagatct gcgagcttgt tgacaccagc ttgaagtctc ctaatggtag
     6181 tcccatcttt gttgaaatgt aattcctttg ctgccatatg tatatctcct tcttaaagtt
     6241 aaacaaaatt atttctagct ccttacacca tgaggccctc aggagcagca gcagcaggag
     6301 ccttgggttt cggtttgtca acaacaatgg cctgagtggt cagtaccatt cctgctacgg
     6361 aagcagcgtt ctgtagcgca catcttgtca ctttagccgg atcaatcact ccagcttcga
     6421 acagattctc gtaagtatca gtcattgcgt tgtacccatt ctcccaatcg ctgaacataa
     6481 tcttttccac aacaacttct ccttcaactc cagcgttctg agcaataaga gcagctggtg
     6541 acagcaatgc cttttgtact atgtcagctc ccaaacgttc atcagcatcc tcaaaagtct
     6601 ccttaatggc gggaataaca gtggagagat gcaccaaagc agcaccacca cctggaacta
     6661 tgccttcctc gatagcagca aatgttgcgt tctttgcatc ctcgatacga agcttacggt
     6721 cctcaagctc agtttcagtt gctgctccga ctttaatgac agcaacacct ccagatagct
     6781 tcgcaattct ctcagcgagc ttctctgagt catacacaga atcagtctca aatagttctt
     6841 tcttcagctg agcaatccga gcttgtaatt cgtccttgga agctgcatct gcaatgaggg
     6901 tagtcgaatc tttgctaatc gtgacttttc gggcaatacc caattgatct atggttgcgt
     6961 tttcaaccag taagctcatg tccatggcta ggtactcagc tcctgtcaag attgcaatat
     7021 cttgaagcat ggcttttctt ctttctccaa atcctggcgc tttaacggca acaacattga
     7081 ggacaccacg aagcttgttc acgacaaggg tagctaaggc ttcaccagta acatcctctg
     7141 caataatcaa caacggagca cgaagctgag tggtcttctc caaaataggg atgatgtctt
     7201 tgatcgcagt gatcttctga tcagtgatca ataccctagc attctcaaac tcagctagta
     7261 gtttctcagg gtttgtaaca aactgaggcg agatgtaacc tctgtcaatc tccatccctt
     7321 cttcaacttc gaccgtggtt tcaaaagaag atgaagattc aatggacaaa acaccatcag
     7381 gtccaacttt gtcgatggca tcagcaatca ttgaccctat aaggtcatca tttccagcag
     7441 agatagaagc cacagctctg atgtcatcac gacctttcac gggcctagct ttcttctgaa
     7501 gctcttcgat cagaccttga acagtcttat caattcccct cttgagtgaa acgggattcg
     7561 caccagaagt gacgctcaac aatccatgtt tgattatctc ccgagcaagg atagacgcag
     7621 tggttgtccc atcaccagca gagtcattag tcttactcgc aacctcacga ataagcgctg
     7681 caccagcatt ttccatggca ttaggtaact caatagctct agcaatggtg actccatcat
     7741 tcacaacctt aggacttcca aattcatcca acacaacatt cctccctctg gggccaagcg
     7801 taagaccaac acaatcagca agcttatcaa taccagcttg tagagcagct ctagagtgct
     7861 ggtcaaaagc tatttccttt acattcatat gtatatctcc tactgcacct attgttggtg
     7921 aagtgctcgt gaaaacacct aaacggactg gctgtttcct gtgtgaaatt gttatccgct
     7981 cacaattcca cacattatac gagccgatga ttaattgtca acagctcatt tcagaatatt
     8041 tgccagaacc gttatgatgt cggcgcaaaa aacattatcc agaacgggag tgcgccttga
     8101 gcgacacgaa ttatgcagtg atttacgacc tgcacagcca taccacagct tccgatggct
     8161 gcctgacgcc agaagcattg gtgcaccgtg cagtcgatga taagctgtca aacatgagaa
     8221 ttgtgcctaa tgagtgagct aacttacatt aattgcgttg cgcgacacca tcgaatggtg
     8281 caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa
     8341 tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
     8401 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
     8461 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
     8521 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
     8581 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
     8641 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
     8701 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
     8761 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
     8821 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
     8881 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
     8941 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
     9001 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
     9061 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
     9121 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc
     9181 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
     9241 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
     9301 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     9361 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     9421 gtgaagggtc cctaagtctc ctcagcaaaa cgaaaggccc agtctttcga ctgagccttt
     9481 cgttttattt gaccggatgt cctcttcctc ctgggctgac ttcaggtgct acatttgaag
     9541 agataaattg cactgaaatc tagagcggtt cagtagaaaa gatcaaagga tcttcttgag
     9601 atcctttttt tctgcgcgta atcttttgcc ctgtaaacga aaaaaccacc tggggaggtg
     9661 gtttgatcga aggttaagtc agttggggaa ctgcttaacc gtggtaactg gctttcgcag
     9721 agcacagcaa ccaaatctgt ccttccagtg tagccggact ttggcgcaca cttcaagagc
     9781 aaccgcgtgt ttagctaaac aaatcctctg cgaactccca gttaccaatg gctgctgcca
     9841 gtggcgtttt accgtgcttt tccgggttgg actcaagtga acagttaccg gataaggcgc
     9901 agcagtcggg ctgaacgggg agttcttgct tacagcccag cttggagcga acgacctaca
     9961 ccgagccgag ataccagtgt gtgagctatg agaaagcgcc acacttcccg taagggagaa
    10021 aggcggaaca ggtatccggt aaacggcagg gtcggaacag gagagcgcaa gagggagcga
    10081 cccgccggaa acggtgggga tctttaagtc ctgtcgggtt tcgcccgtac tgtcagattc
    10141 atggttgagc ctcacggctc ccacagatgc accggaaaag cgtctgttta tgtgaactct
    10201 ggcaggaggg cggagcctat ggaaaaacgc caccggcgcg gccctgctgt tttgcctcac
    10261 atgttagtcc cctgcttatc cacggaatct gtgggtaact ttgtatgtgt ccgcagcgcc
    10321 cgccgcagtc tcacgcccgg agcgtagcga ccgagtgacg gccttaattt ggctt
//
