LOCUS       sequence-472465-       10387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses A. thaliana Cpn60-alpha Cpn60-beta Cpn20 Raf2 RbcX Bsd2 O.
            sativa Raf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10387)
  AUTHORS   Archer J, Kathpalia M, Lee BU, Li S, Wang T
  TITLE     Effects of chaperone selectivity on the assembly of plant Rubisco 
            orthologs in E. coli.
  JOURNAL   J Exp Bot. 2025 Mar 31:eraf140. doi: 10.1093/jxb/eraf140.
  PUBMED    40163664
REFERENCE   2  (bases 1 to 10387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Mar 31:eraf140. doi: 10.1093/jxb/eraf140."
COMMENT     Sequence Label: pTW334h
FEATURES             Location/Qualifiers
     source          1..10387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             86..877
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        join(1194..1199,1200..1215,1216..1222)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1230..1246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4522..4544
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             5811..5819
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             6237..6259
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    7981..7997
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8005..8011,8012..8027,8028..8033))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        8276..8353
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8354..9436
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8373..8392)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      9593..10331
                     /direction=RIGHT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     promoter        join(10381..10387,1..85)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
       61 tcaataatat tgaaaaagga agagtatgag ggaagcggtg atcgccgaag tatcgactca
      121 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      181 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
      241 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      301 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      361 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      421 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      481 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      541 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
      601 atggaactcg ccgcccgact gggctggaga tgagcgaaat gtagtgctta cgttgtcccg
      661 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
      721 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
      781 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
      841 cgtgaaaggc gagatcacca aggtagtcgg caaataaacg ccatgggcat taaaaaatgc
      901 cctcttgggt tatcaagagg gtcattatat ttgctgagga gtgccgttaa tgcgcaacgc
      961 aattaatgta agttagctca ctcattaggc acaattctca tgtttgacag cttatcatcg
     1021 actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg
     1081 tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa
     1141 tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa
     1201 ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     1261 cagccagtcc gtttaggtgt tttcacgagc acttcaccaa caacgcgtac aactaggaga
     1321 tataccatgg gtatggacca actgtatcag ccgtttcacc cgccgccgag tccactccct
     1381 gataaataca aagacttaga tctagggcaa cgcctggctg tactccgtga tcgactgggt
     1441 ctgtggcatg aatacgcgcc gctgatctcg gcgctatcac gtgaaggatt cactccgtcc
     1501 tctattgaag aagctaccgg tattagtggt gttgaacaga attcggtggt ggtcgcgaca
     1561 caggtgcgcg acagcctggt ggcggatgag ggtggcttcc cagccgaact cttgcgctat
     1621 ttcgatagct atggaggccc ggagctcttg tatgaattac gctttctgaa cgcacgccag
     1681 cgtgccgacg cggcccgcca tgccattgat cgtcgtcttg aacctcgagg tgtgcgtgaa
     1741 ctggcgcgca gcatgaagga ttttccgcag cgccgcgggg acgacggttg ggaggctttc
     1801 acccgcgata atcccggtga ctgcctggca tttgctcgtt ttcgtcagtc ccgcgaagcc
     1861 atcgatgcgg aagatagcgt tgcggagctg gaacgcgcct tggaagtagt tgacaccgag
     1921 cctgctcgcg cgcgtgtaga ggcagaactg gatcgcgcgc gccgcaaagc cgcgggcgaa
     1981 gaagtggatg atgaagacgg ggcagcaaac gccgcggccg cggcgtctcg gcccgctgta
     2041 ccggtggtcc gccttatgta cggggaagtg gcagaagcca ccacggtgct gctgttgccc
     2101 gttgtgcgcg agggcgacgg cggtgaagcg cttgcccacg cgccgagacg tactcgtacc
     2161 gacgcggatt taggcatggt ggaagtggat aagggctgga cgcgctgggc cgtcgttcca
     2221 ggctggggac cggttgcaga ggttgcaggc gaagcggttg tgattgagct ggcagatggc
     2281 cgtacgcttc cgtggaggag cgcggaggcg gaacgtgtcc tggtcgtcgc caaccgtggc
     2341 cggcgggaag tagtggagga cggcatttac gtcgtggaac gtgaaggacg tctggttgta
     2401 gagaaaggtc gaaaattagc cgcagagggc gtgggcgaag cagccgccga agtgttagtt
     2461 gtagtcaggc ctccgcgcga tgaagatgat atgatctcag atgatgaatg ggattaaatt
     2521 acaaaaatta ggagatatac atatgatgtc taatctggcg caggattttc ttggtgactt
     2581 cggagctcgt gacccttacc cggaggagat agcgagtcaa ttcggagata aagtgttggg
     2641 atgccaaagc actgagcaca agattttgat accaaacgca tctgttttgt ctctctccca
     2701 gcttcagtgt tcccctgttt cgtcttcaca gcctcctttg tccggcgatg atgccagaac
     2761 tctcctccac aaggttttgg gatggagtat agtggataat gaagcgggtg gtctgaaaat
     2821 aaggtgtatg tggaaagtga gggattttgg gtgcggtgtt gaactcataa acaggatcca
     2881 taaggttgct gaagcttctg gtcattaccc ttctcttcac ttggaaagtc ctacccaagt
     2941 tcgagctgaa ctatttacct cttctatcgg agggttgagc atgaatgatt tcataatggc
     3001 ggctaaaata gatgatatca agacttctga tctttcccct aggaaaagag cttgggcgtg
     3061 acggctgaaa ttaatgagga gatatacata tggtatcgag aattgcgaat cgaaagagca
     3121 aaaagctctt gattgtgaat gaggatgttg ctggtaatta cgacgatacc ttcggtgatg
     3181 tacaaaagca aattgttaat tattttacat acaaagctgt gaggacggtt cttcatcagc
     3241 tgtacgaaat gaatcctcct caatacactt ggttctataa tcatatcata acaaacagac
     3301 cgacggatgg caaacgtttc ctccgtgccc tcggcaagga gagtcaggag cttgcagaaa
     3361 gagtgatgat cacgcgtctt cacttgtatg gcaaatggat caagaaatgc gaccatggga
     3421 agatatacca ggaaatatcg gatgaaaact tggcgttgat gcgtgaacgc ctgatggaga
     3481 cagtgatatg gccttccgat gacacaaact caaggtaatt cagctaagac actgcacagg
     3541 agatatacat atgatggccg caaacaataa tcctcagggc actaaaccaa acagtttagt
     3601 ttgtgcaaat tgtgaagggg aaggttgtgt ggcatgttct caatgcaaag gaggtggtgt
     3661 gaatttgatt gatcatttca atggtcagtt taaagctggt gcactctgtt ggctttgcag
     3721 aggtaaaaag gaggtgttgt gtggggactg caatggagct ggtttcattg gtgggttctt
     3781 gagcaccttc gatgagtaga gtcagtaggg ccctaaaaaa aaaccccgcc cctgacaggg
     3841 cagggttttt tttggcgaaa aaaccccgct gaagcggggt tttttgcgta cattggctcc
     3901 gtctaagaaa gtatagccat cacatctgaa gctctgaggg caatgtagtt ggaaccatct
     3961 ttgcccttga agtcgttacc agcatacttg gagtaaagta ctgtgcttcc ggttgatact
     4021 ggtagaggcg taattttacc ttcctcgtct agggaacccg gtccaactgc tatcacggtg
     4081 ccaatagaag gcttctcttt ggtagtctcg gttaacaaca accctccagc tgttttctcc
     4141 tccgcctcag caaccttaat aaagactcgg tcattcaaag gtttgagatc tttgatgtcc
     4201 tctgtctcaa gaatgccaac aatatcatct tccttgagga taagatgctt cacatcattg
     4261 aactccacct cagttcctgc gtatttggag tagataattt gtgctccagt agggacagtg
     4321 atatcaattt tgttcttccc aatagttctt ccttcaccca cagcaacgac ttcacctcct
     4381 tgaggttttg attgagcagt ggatggaagt aagataccac ctaaagtctt ctcctctgcc
     4441 tccttgatct tcaccaaaac tcgatctccc aatggcttaa ttgaagtata cttaggggca
     4501 acaacagagg ccatatgtat atctccttct taaagttaaa caaaattatt tctagagggc
     4561 aattgttatc cggtcgacgt cgaccttagt atccgtaacc tgagttgtcc attgggttgc
     4621 cgacgggaac tggttcaggt tccttgatct caacaacaac gcagtccgac atcaagaaag
     4681 tctttgcaac cgaagctgcg tgttccaagc aacatctcac aacctttgtt ggatcgatga
     4741 ttcctgcagc catcaaatcc tcgtacttgc cggttgcagc attgtaaccg aatttcacgt
     4801 tatcgttgga aagcaccttc tcgctaacta cacttccatt aacaccagca ttcttggcga
     4861 ttagttttag ggggtaactt agtgctcttt taactatatc cgctccaacc ttttcttcat
     4921 cattatcaag cgtggcttta atggcatcaa ccttggaagc aagacgaagc agagtgcaac
     4981 caccaccaac aacaatacct tcctcgacag cggcctttgt tgcattaaga gcatcttcaa
     5041 ctctcaactt cttctctttg agttctgtct cagtttgtgc tccaacctga atcacagcaa
     5101 ctccaccaga gagctttgca attctctcat tgagtttctc cttctcgtaa tcttgctccg
     5161 cttgctcaat aagattttta atctgtgtaa cacgcttttt cactgcatcc tgtgtgcttc
     5221 catcacccac tatggtcgag gtttcctttg tgaggacaac ctttgaagca ttaccaagaa
     5281 cctcttttcc agctttgtca agtgaaagac caacttcctc acggatcaca gttgctccag
     5341 ttagaatggc aatatcatca aggtattggc tcttgcgctc tccaaaccct ggagctctga
     5401 gagctgctat cttcagtgta cctctaagct tgtttacaac aagagtcgct aaagcttctt
     5461 gttcaatgtc ttcagcaata attaatattg ggtatccgcc tctaattgca tcctccaaaa
     5521 caccaacaag atcccttgca ttggtaattt tcttgtcaac gagaagtaac ttgcaattgt
     5581 cgaactcgac ggacattttc tcgctgtctg tcacaaagta aggagagata taaccacggt
     5641 caaattgcat tccttctacg acgtagaggt tgttctcggc acttttaccc tcctcaaggg
     5701 taaccacacc tttcctgccc actttgctca ttgcttcagc aatcatattt ccaatttcgt
     5761 cgttgttacc cgcactaaca gctgcaacat ctgcaagttc actgtcttca acctccttag
     5821 acattttctt gagctcggta accaaagcct ttgctgtctt ctcaatacct ctggtgatca
     5881 aaacagggtt tgcaccagca gctaccacct tgacaccctc agcgataaaa ccttgtgcaa
     5941 gaacaacaga tgttgttgta ccgtcaccag ccaagtcatt ggttttggca gccgcttgcc
     6001 tcacaagctt agcgccaatg ttctcaaccg ggtcttccaa ctcaacctct cttgcaacag
     6061 tcacaccatc attaacaatt cgtggtgatc catacttgct ctcaagaaca acatttcgtc
     6121 ctttgggtcc aagtgtaacg ccaacaagat ctgcgagctt gttgacacca gcttgaagtc
     6181 tcctaatggt agtcccatct ttgttgaaat gtaattcctt tgctgccata tgtatatctc
     6241 cttcttaaag ttaaacaaaa ttatttctag ctccttacac catgaggccc tcaggagcag
     6301 cagcagcagg agccttgggt ttcggtttgt caacaacaat ggcctgagtg gtcagtacca
     6361 ttcctgctac ggaagcagcg ttctgtagcg cacatcttgt cactttagcc ggatcaatca
     6421 ctccagcttc gaacagattc tcgtaagtat cagtcattgc gttgtaccca ttctcccaat
     6481 cgctgaacat aatcttttcc acaacaactt ctccttcaac tccagcgttc tgagcaataa
     6541 gagcagctgg tgacagcaat gccttttgta ctatgtcagc tcccaaacgt tcatcagcat
     6601 cctcaaaagt ctccttaatg gcgggaataa cagtggagag atgcaccaaa gcagcaccac
     6661 cacctggaac tatgccttcc tcgatagcag caaatgttgc gttctttgca tcctcgatac
     6721 gaagcttacg gtcctcaagc tcagtttcag ttgctgctcc gactttaatg acagcaacac
     6781 ctccagatag cttcgcaatt ctctcagcga gcttctctga gtcatacaca gaatcagtct
     6841 caaatagttc tttcttcagc tgagcaatcc gagcttgtaa ttcgtccttg gaagctgcat
     6901 ctgcaatgag ggtagtcgaa tctttgctaa tcgtgacttt tcgggcaata cccaattgat
     6961 ctatggttgc gttttcaacc agtaagctca tgtccatggc taggtactca gctcctgtca
     7021 agattgcaat atcttgaagc atggcttttc ttctttctcc aaatcctggc gctttaacgg
     7081 caacaacatt gaggacacca cgaagcttgt tcacgacaag ggtagctaag gcttcaccag
     7141 taacatcctc tgcaataatc aacaacggag cacgaagctg agtggtcttc tccaaaatag
     7201 ggatgatgtc tttgatcgca gtgatcttct gatcagtgat caatacccta gcattctcaa
     7261 actcagctag tagtttctca gggtttgtaa caaactgagg cgagatgtaa cctctgtcaa
     7321 tctccatccc ttcttcaact tcgaccgtgg tttcaaaaga agatgaagat tcaatggaca
     7381 aaacaccatc aggtccaact ttgtcgatgg catcagcaat cattgaccct ataaggtcat
     7441 catttccagc agagatagaa gccacagctc tgatgtcatc acgacctttc acgggcctag
     7501 ctttcttctg aagctcttcg atcagacctt gaacagtctt atcaattccc ctcttgagtg
     7561 aaacgggatt cgcaccagaa gtgacgctca acaatccatg tttgattatc tcccgagcaa
     7621 ggatagacgc agtggttgtc ccatcaccag cagagtcatt agtcttactc gcaacctcac
     7681 gaataagcgc tgcaccagca ttttccatgg cattaggtaa ctcaatagct ctagcaatgg
     7741 tgactccatc attcacaacc ttaggacttc caaattcatc caacacaaca ttcctccctc
     7801 tggggccaag cgtaagacca acacaatcag caagcttatc aataccagct tgtagagcag
     7861 ctctagagtg ctggtcaaaa gctatttcct ttacattcat atgtatatct cctactgcac
     7921 ctattgttgg tgaagtgctc gtgaaaacac ctaaacggac tggctgtttc ctgtgtgaaa
     7981 ttgttatccg ctcacaattc cacacattat acgagccgat gattaattgt caacagctca
     8041 tttcagaata tttgccagaa ccgttatgat gtcggcgcaa aaaacattat ccagaacggg
     8101 agtgcgcctt gagcgacacg aattatgcag tgatttacga cctgcacagc cataccacag
     8161 cttccgatgg ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgat gataagctgt
     8221 caaacatgag aattgtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgcgacac
     8281 catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
     8341 cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
     8401 ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
     8461 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
     8521 ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
     8581 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
     8641 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
     8701 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
     8761 ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat
     8821 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
     8881 attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct
     8941 gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
     9001 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
     9061 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
     9121 cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac
     9181 cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
     9241 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
     9301 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac
     9361 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     9421 ggaaagcggg cagtgaaggg tccctaagtc tcctcagcaa aacgaaaggc ccagtctttc
     9481 gactgagcct ttcgttttat ttgaccggat gtcctcttcc tcctgggctg acttcaggtg
     9541 ctacatttga agagataaat tgcactgaaa tctagagcgg ttcagtagaa aagatcaaag
     9601 gatcttcttg agatcctttt tttctgcgcg taatcttttg ccctgtaaac gaaaaaacca
     9661 cctggggagg tggtttgatc gaaggttaag tcagttgggg aactgcttaa ccgtggtaac
     9721 tggctttcgc agagcacagc aaccaaatct gtccttccag tgtagccgga ctttggcgca
     9781 cacttcaaga gcaaccgcgt gtttagctaa acaaatcctc tgcgaactcc cagttaccaa
     9841 tggctgctgc cagtggcgtt ttaccgtgct tttccgggtt ggactcaagt gaacagttac
     9901 cggataaggc gcagcagtcg ggctgaacgg ggagttcttg cttacagccc agcttggagc
     9961 gaacgaccta caccgagccg agataccagt gtgtgagcta tgagaaagcg ccacacttcc
    10021 cgtaagggag aaaggcggaa caggtatccg gtaaacggca gggtcggaac aggagagcgc
    10081 aagagggagc gacccgccgg aaacggtggg gatctttaag tcctgtcggg tttcgcccgt
    10141 actgtcagat tcatggttga gcctcacggc tcccacagat gcaccggaaa agcgtctgtt
    10201 tatgtgaact ctggcaggag ggcggagcct atggaaaaac gccaccggcg cggccctgct
    10261 gttttgcctc acatgttagt cccctgctta tccacggaat ctgtgggtaa ctttgtatgt
    10321 gtccgcagcg cccgccgcag tctcacgccc ggagcgtagc gaccgagtga cggccttaat
    10381 ttggctt
//
