LOCUS       sequence-472486-       10426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses O. sativa Cpn60-alpha Cpn60-beta Cpn20-2 Raf1 Raf2 RbcX 
            Bsd2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10426)
  AUTHORS   Archer J, Kathpalia M, Lee BU, Li S, Wang T
  TITLE     Effects of chaperone selectivity on the assembly of plant Rubisco 
            orthologs in E. coli.
  JOURNAL   J Exp Bot. 2025 Mar 31:eraf140. doi: 10.1093/jxb/eraf140.
  PUBMED    40163664
REFERENCE   2  (bases 1 to 10426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Mar 31:eraf140. doi: 10.1093/jxb/eraf140."
COMMENT     Sequence Label: pTW386c
FEATURES             Location/Qualifiers
     source          1..10426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(57..795)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(952..2034)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1996..2015
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(2035..2112)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        join(2355..2360,2361..2376,2377..2383)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    2391..2407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4132..4154
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             5844..5866
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    9181..9197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9205..9211,9212..9227,9228..9233))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             complement(9550..10341)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(join(10342..10426,1..7))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagccaaatt aaggccgtca ctcggtcgct acgctccggg cgtgagactg cggcgggcgc
       61 tgcggacaca tacaaagtta cccacagatt ccgtggataa gcaggggact aacatgtgag
      121 gcaaaacagc agggccgcgc cggtggcgtt tttccatagg ctccgccctc ctgccagagt
      181 tcacataaac agacgctttt ccggtgcatc tgtgggagcc gtgaggctca accatgaatc
      241 tgacagtacg ggcgaaaccc gacaggactt aaagatcccc accgtttccg gcgggtcgct
      301 ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg atacctgttc cgcctttctc
      361 ccttacggga agtgtggcgc tttctcatag ctcacacact ggtatctcgg ctcggtgtag
      421 gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg ttcagcccga ctgctgcgcc
      481 ttatccggta actgttcact tgagtccaac ccggaaaagc acggtaaaac gccactggca
      541 gcagccattg gtaactggga gttcgcagag gatttgttta gctaaacacg cggttgctct
      601 tgaagtgtgc gccaaagtcc ggctacactg gaaggacaga tttggttgct gtgctctgcg
      661 aaagccagtt accacggtta agcagttccc caactgactt aaccttcgat caaaccacct
      721 ccccaggtgg ttttttcgtt tacagggcaa aagattacgc gcagaaaaaa aggatctcaa
      781 gaagatcctt tgatcttttc tactgaaccg ctctagattt cagtgcaatt tatctcttca
      841 aatgtagcac ctgaagtcag cccaggagga agaggacatc cggtcaaata aaacgaaagg
      901 ctcagtcgaa agactgggcc tttcgttttg ctgaggagac ttagggaccc ttcactgccc
      961 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1021 agaggcggtt tgcgtattgg gtgccagggt ggtttttctt ttcaccagtg agacgggcaa
     1081 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
     1141 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
     1201 gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc
     1261 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
     1321 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
     1381 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc
     1441 cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg
     1501 acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat
     1561 actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc
     1621 agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac
     1681 gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac
     1741 catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat
     1801 ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt
     1861 gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc
     1921 cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt
     1981 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac
     2041 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcacca
     2101 ttcgatggtg tcgcgcaacg caattaatgt aagttagctc actcattagg cacaattctc
     2161 atgtttgaca gcttatcatc gactgcacgg tgcaccaatg cttctggcgt caggcagcca
     2221 tcggaagctg tggtatggct gtgcaggtcg taaatcactg cataattcgt gtcgctcaag
     2281 gcgcactccc gttctggata atgttttttg cgccgacatc ataacggttc tggcaaatat
     2341 tctgaaatga gctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg
     2401 ataacaattt cacacaggaa acagccagtc cgtttaggtg ttttcacgag cacttcacca
     2461 acaataggtg cagtaggaga tatacatatg gacgccaagg acatcgcctt cgaccagaaa
     2521 tcccgggcgg cgctccaggc cggcgtcgag aagctcgcca acgccgttgg cgtcaccctc
     2581 ggcccacgag ggagaaacgt agtgcttgat gagtacggca gtcccaaagt ggtaaatgat
     2641 ggagtcacca ttgcccgggc tattgagcta tatgatccga tggaaaatgc tggtgcagca
     2701 ttgatccgtg aggttgctag caagaccaat gattctgctg gtgatgggac tacaaccgcc
     2761 tctgtccttg ctcgtgaaat catcaagctg ggccttttga gtgtaacttc tggtgcaaac
     2821 cctgtatcac ttaagaaagg aatagacaaa acagtgcatg gtcttattga agagctggag
     2881 aagaaggcta gaccagtcaa gggcagtggt gatatcaaag ctgttgcctc tatctctgct
     2941 ggcaacgatg aacttattgg atctatgatt gctgatgcta ttgacaaagt gggtcctgat
     3001 ggtgtccttt caattgagtc ttcatcatct ttcgagacta ctgttgatgt tgaagaagga
     3061 atggagattg accgtggata catttcccct caatttgtga caaacctcga gaaatcgatc
     3121 gtagagtttg agaatgctaa ggttcttatt actgatcaga agatcaccag cataaaggag
     3181 attcttccaa tcttggagaa gacaacacag ttgagagctc cactgtttat tattgctgag
     3241 gacattactg gcgaagcttt ggcaaccctt gttgttaata agctccgagg aattctcaat
     3301 gttgcagcaa tcaaagcccc aagttttggt gagcgacgca aggctgtcct tcaggatatt
     3361 gccattgtga caggcgctga atttctagca aaagacctgg gtttgctggt tgagaatgca
     3421 actgaagagc aacttgggac agcgagaaaa gtcacaatac atcagactac aacaaccctc
     3481 atagcggatg cagctagcaa agatgagatt caggcaaggg ttgcacagct aaagaaggaa
     3541 ctctccgaaa ctgattcgat atatgataca gagaaattgg ctgagaggat tgcaaagctt
     3601 tctggtggtg tggctgtcat caaggttggg gctgcaactg agactgagct tgaggaccgc
     3661 caactacgta tcgaggatgc gaagaatgca actttcgcag ctattgagga gggcattgtt
     3721 cctggaggtg gtacagcgta tgtgcatctt tcaactaccg tccctgcgat caaagaaaca
     3781 atcgaagacc atgatgagcg ccttggtgca gacattattc aaaaggcgtt ggtagctccc
     3841 gcttcactga ttgcccacaa tgctggagtg gaaggggaag tggtagttga gaagatcaag
     3901 gacggcgagt gggaagtcgg gtacaatgcg atgaacgaca agtatgagaa cttgattgag
     3961 gcgggtgtta ttgacccagc taaggtgacg agatgcgcgc tccagaatgc cgcctcggtg
     4021 gctggaatgg tccttaccac acaggccatt gttgtcgaga agccgaagcc caaggctcct
     4081 gttgctgagc cggcagaggg aacccttacc gtctaaggag ctagaaataa ttttgtttaa
     4141 ctttaagaag gagatataca tatggccaag gagttgtact tcaacaagga tggctcggct
     4201 atcaagaagc ttcagactgg agtaaacaag cttgctgacc ttgttggagt tacactggga
     4261 ccaaagggaa ggaatgttgt tttggagagc aagtatgggt cccctaggat tgtcaatgat
     4321 ggtgttacag ttgcaagaga ggttgaactg gaggatcctg ttgaaaatat tggagctaag
     4381 ttggttaggc aagcagcagc aaagaccaat gatttagctg gagatggcac aactacttct
     4441 gttgtgcttg ctcaggggct gattgctgag ggtgttaagg tcgttgcagc tggtgccaat
     4501 cctgtgcaaa ttacacgtgg tatcgagaaa actgcgaaag cactagttga agagctaaag
     4561 aaattgtcaa aggaggttga agacagtgag cttgcggatg ttgctgcagt cagtgctggc
     4621 aacaattatg aaatcggtaa tatgatagca gaggctatga gcaaggttgg caggaagggt
     4681 gtggttaccc ttgaagaagg gagaagttct gagaataatc tctatgttgt ggagggaatg
     4741 cagtttgaac gtggttatat ctccccgtat tttgtaactg acagcgagaa aatgtctgca
     4801 gaatatgaga actgcaagct tcttttggtg gacaaaaaaa tcaccaacgc aagggatctt
     4861 atcaatgttt tggaggaggc cataagaggt gcatacccaa ttctgataat tgctgaggat
     4921 attgagcagg aggctcttgc tactcttgtt gtcaacaagc ttagagggtc attgaaaatt
     4981 gccgctatca aagcccctgg ttttggtgag cgcaagaccc agtacttgga tgatattgcc
     5041 attctaactg gagcaactgt aatcagggat gaagttgggc tgtcacttga caaggcagac
     5101 aaatcagttt taggaacggc tgcaaaggtg gtccttaaca aagagtcaac aacaattgtt
     5161 ggtgatggca gtacacagga agaagtgact aagagggttg cacaaataaa aaatctcatc
     5221 gaggctgccg aacaagaata cgagaaggaa aagctcaatg agaggattgc aaagcttgct
     5281 ggtggtgttg cagtcattca ggtgggagca caaacagaga ctgaactaaa agagaagaag
     5341 ttgagagttg aggatgccct aaatgctacc aaggctgctg ttgaggaagg tattgttgtt
     5401 ggtggagggt gcactcttct gagactggca gctagagttg acgccatcaa ggacaacctg
     5461 gagaatgatg agcagaaggt tggagctgaa attgtaagga gagcgctgag ctacccgctt
     5521 aaattgattg ctaagaatgc tggtgttaat ggaagcgttg ttaccgagaa ggttctctct
     5581 aacgataact tcaagtttgg ttacaatgct gcaactggac agtacgagga tttgatggct
     5641 gctggtatca ttgaccccac taaggtggtg agatgttgtc ttgagcatgc tgcgtcggtg
     5701 gccaagacat tcctcacatc ggatgtcgtc gtcgtcgaga tcaaggagcc tgagcctgcg
     5761 cccgtcacca acccgatgga caactccggc tacgggtact gaggtcgacg tcgaccggat
     5821 aacaattgcc ctctagaaat aattttgttt aactttaaga aggagatata catatggccg
     5881 ccacggtcgt caccccaaag tatacctcgc tcaagcctct gggagacaga gtgcttgtga
     5941 agcttggtgc tgcagaggag aagactgttg gtgggattct gcttccgtca accgcacagt
     6001 ctaagcctca gggaggtgag gttgttgctg ttggagaggg aagaactatt ggggataaga
     6061 aagttgaggt cagcttgcag attggggctg aagttgtata ttcaaaatat gctgggactg
     6121 aggtgcaatt taacgacacc aaacatctta ttctaaaaga ggatgatatc attggtgttc
     6181 ttgagactga tgatgtcaaa gatatgaagc cccttaatga ccgggttctc atcaaggttg
     6241 ctgaggctga agacaaaact gctggtggcc ttattctcac tgaaaccact aaggagaagc
     6301 catcaatcgg aacagttgta gccgttggtc caggccctct tgatgatgaa ggcaagaggc
     6361 agccattgtc agtttcagcg ggcagcactg tgatgtactc caagtacgca ggcagtgagt
     6421 tcaagggagc tgatggcacc aactacattg tcttgagagt atcagatgtg atggctgtcc
     6481 tatcttgaac ggagccaatg tacgcaaaaa accccgcttc agcggggttt tttcgccaaa
     6541 aaaaaccctg ccctgtcagg ggcggggttt ttttttaggg ccctactgac tctattcggc
     6601 ggtgctatcg ctagtggaca tgaaacctcc taagaagccg gcgccgttgc acgagccgca
     6661 gagaatttca cgcttaccac ggcacagcca acatagcgcg cccgctttaa agcgcccatt
     6721 aaaatgatct acggaattaa caccgtctcc tttacactgg ttgcagacga ttgcaccatt
     6781 gccttcgcag tcctgacaca ccagtgacga gccttttggc gggctttttt cggtttgatc
     6841 gttctctgta gccatcatat gtatatctcc tgtgcagtgt cttagctgaa ttacccaatc
     6901 ttctcggtat tggtgtcatc cgtcggccaa attaccgttt ccatcagacg ctccctcata
     6961 agtgccaaat tttcgtcgct gatgcgttca tacatcatcg catgatcaca tttctttatc
     7021 catttgctgt acaggtgaag gcgagtaatc atcacgcgtt cagctaactc ttgtttttct
     7081 ttacccaggg ctcgcagaaa gactttccca tcggtgggct tattcaccgc tacaaagtta
     7141 tacagccaac ggtacgaagg tgggttcatt tcatataatt gagtcaaaac ggttttaacg
     7201 gctttgtacg tgaagacgtt aattagctga acatggatat cctcgaagcc ttcatcatag
     7261 gtgccgccca ggttcatatg tatatctcct cattaatttc agccgttacg cccacacccg
     7321 tttcttagga atcagatcca ccgttttaac tttgtcaata cgtgcggcga caatgaagtc
     7381 gtttactgtc agtccgcccg cgctgggggt ctgaagttcg gcacgcacct ggtttggtgc
     7441 ttcaaagacg acggtcgccg gcgcaccgtc tactgccgcg ttaatgcgtg ccaccaactc
     7501 atgaccgcac gcttcatcac gcaccttcca tacgcactgg aggcgcatgc catctgcatc
     7561 atccaacagt cgccagccca caactttgtg aagtaaacgc ctagcatccg cggcgtcaac
     7621 cggtgcagga ttcggttcca gcgggaggcg ggctattgat aacaccgaca gagttgggat
     7681 caagatatta tgcagcgtat ctacattacc taaaacacgt tcgccaaagt tgctctcaat
     7741 ctcttccgga aacgggtcgc gcgccccgaa atctcccagc agatcggcca tcatatgtat
     7801 atctcctaat ttttgtaatt taatcccatt catcatctga gatcatatca tcttcatcgc
     7861 gcggaggcct gactacaact aacacttcgg cggctgcttc gcccacgccc tctgcggcta
     7921 attttcgacc tttctctaca accagacgtc cttcacgttc cacgacgtaa atgccgtcct
     7981 ccactacttc ccgccggcca cggttggcga cgaccaggac acgttccgcc tccgcgctcc
     8041 tccacggaag cgtacggcca tctgccagct caatcacaac cgcttcgcct gcaacctctg
     8101 caaccggtcc ccagcctgga acgacggccc agcgcgtcca gcccttatcc acttccacca
     8161 tgcctaaatc cgcgtcggta cgagtacgtc tcggcgcgtg ggcaagcgct tcaccgccgt
     8221 cgccctcgcg cacaacgggc aacagcagca ccgtggtggc ttctgccact tccccgtaca
     8281 taaggcggac caccggtaca gcgggccgag acgccgcggc cgcggcgttt gctgccccgt
     8341 cttcatcatc cacttcttcg cccgcggctt tgcggcgcgc gcgatccagt tctgcctcta
     8401 cacgcgcgcg agcaggctcg gtgtcaacta cttccaaggc gcgttccagc tccgcaacgc
     8461 tatcttccgc atcgatggct tcgcgggact gacgaaaacg agcaaatgcc aggcagtcac
     8521 cgggattatc gcgggtgaaa gcctcccaac cgtcgtcccc gcggcgctgc ggaaaatcct
     8581 tcatgctgcg cgccagttca cgcacacctc gaggttcaag acgacgatca atggcatggc
     8641 gggccgcgtc ggcacgctgg cgtgcgttca gaaagcgtaa ttcatacaag agctccgggc
     8701 ctccatagct atcgaaatag cgcaagagtt cggctgggaa gccaccctca tccgccacca
     8761 ggctgtcgcg cacctgtgtc gcgaccacca ccgaattctg ttcaacacca ctaataccgg
     8821 tagcttcttc aatagaggac ggagtgaatc cttcacgtga tagcgccgag atcagcggcg
     8881 cgtattcatg ccacagaccc agtcgatcac ggagtacagc caggcgttgc cctagatcta
     8941 agtctttgta tttatcaggg agtggactcg gcggcgggtg aaacggctga tacagttggt
     9001 ccgggggtgc ggtcggtggc ggtgtgaaag gcagcttatt gtcacgtgga cctccaccaa
     9061 tcgggcgtcc gtctggtccc aggataacag ccatacccat ggtatatctc ctagttgtac
     9121 gcgttgttgg tgaagtgctc gtgaaaacac ctaaacggac tggctgtttc ctgtgtgaaa
     9181 ttgttatccg ctcacaattc cacacattat acgagccgat gattaattgt caacagctca
     9241 tttcagaata tttgccagaa ccgttatgat gtcggcgcaa aaaacattat ccagaacggg
     9301 agtgcgcctt gagcgacacg aattatgcag tgatttacga cctgcacagc cataccacag
     9361 cttccgatgg ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgat gataagctgt
     9421 caaacatgag aattgtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgcattaa
     9481 cggcactcct cagcaaatat aatgaccctc ttgataaccc aagagggcat tttttaatgc
     9541 ccatggcgtt tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc
     9601 ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct
     9661 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
     9721 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
     9781 cactacattt cgctcatctc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
     9841 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
     9901 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
     9961 gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg
    10021 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac
    10081 ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt
    10141 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
    10201 atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
    10261 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
    10321 ggcgatcacc gcttccctca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10381 ttattgtctc atgagcggat acatatttga atgtatttag aaaaat
//
