LOCUS       sequence-472513-       10225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression of ScVPS38, untagged + ScVPS30 FRK to DDD at 
            430-432, untagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10225)
  AUTHORS   Rostislavleva K, Soler N, Ohashi Y, Zhang L, Pardon E, Burke JE, 
            Masson GR, Johnson C, Steyaert J, Ktistakis NT, Williams RL
  TITLE     Structure and flexibility of the endosomal Vps34 complex reveals the
            basis of its function on membranes.
  JOURNAL   Science. 2015 Oct 9;350(6257):aac7365. doi: 10.1126/science.aac7365.
  PUBMED    26450213
REFERENCE   2  (bases 1 to 10225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2015-10-9"; volume: "350(6257):aac7365. doi"; pages: " 
            10.1126/science.aac7365"
COMMENT     Sequence Label: pYO979
FEATURES             Location/Qualifiers
     source          1..10225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1966..1985
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1966..1984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2070..2187
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     protein_bind    4178..4295
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(6623..6643)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6623..6641)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6662..6678)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6662..6678)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6675..6697)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6686..6702
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6710..6716,6717..6734,6735..6740))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6755..6776
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6893..6910)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7064..7652)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7144..7163)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7823..8614,8615..8683))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8446..8465
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8684..8788)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8815..10157
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9778..9825)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10198..10216
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     1981 tagggcgaat tgggtaccgc tatgacatga ttacgaattg gcgcgccggc cggccggatc
     2041 tttgcaaaga tccaaaaaaa gcatgcgtac ggattagaag ccgccgagcg ggtgacagcc
     2101 ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc
     2161 agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact agcttttatg
     2221 gttatgaaga ggaaaattgg cagtaacctg gccccacaaa cctcaaatga acgaatcaaa
     2281 ttaacaacca gatatctcga ctgaaaaaaa aggtttaaac cagttccctg aaattattcc
     2341 cctacttgac taataagtat ataaagacgg taggtattga ttgtaattct gtaaatctat
     2401 ttcttaaact tcttgaattc tacttttata gttagtcttt tttttagttt taaaacacca
     2461 agaacttagt ttcgaataaa cacacataaa taaaccatgg gcatgaaacg gttcttactc
     2521 agtcgaagac agagacacct tcgtatgatt tgctttcaca atataagtct ctttagagct
     2581 aatggtgact ccaaattgat caaggaatat ggtgatggat ttataccatg tttctttatc
     2641 ttggaatcca taagagggga gctgctttat gtgagcgagg tacaatcagg ttctttgcgt
     2701 aaactctctt ttcaggaatt accaaaacta actggtgcat ctactatgat tgtcctaaaa
     2761 ttggtaggac tagttccgag tgatattttg tgtaccataa gttcagataa aaatggtatc
     2821 attgacgata aatggtgcgt tttatgtacc tacacgatag atcttaacaa gttacaaccc
     2881 ataaatgaag acacagttct aattacaggc accaacgcac cagtacttga tttaatagat
     2941 ggatcctaca cattggctgc agaaaaaatt aagccactaa agggccttgt tagttctcac
     3001 aaaaggaata taagtcaagt caagatcaaa ttttcattgg catatagctc tttactaaaa
     3061 ttaaacaaac tattggagta ttcttcccaa gtccatgagg aaatcaatga aatttccagc
     3121 aaaatagaag atgacttttt gtcactcaag aatcaaaatc attggtatat gagaacagta
     3181 cagaagtcca tagaaacgct agaaaaggaa gtcctacaaa ggaaaaaaag caagaaaaat
     3241 atagaaatgg cgcaactcga gagtaatgat actatcaacc attctaaaac agaactgtct
     3301 ctgatgtcgc aggacgagtc tataaatgat gactatggaa gcatatattc tagatttgtg
     3361 caaataaaag ataggttgga ccaactgagg tttaagaaac tgtatcagct gatagggatc
     3421 ttccattcaa cggatttatt taattcagat agaggctata tttactttga aaaaccttct
     3481 agcgtaaatg atgttattaa taggctcaag ctaaaacctt tgaatataga gatattactt
     3541 agacaagccg gcgaatccac aaagcacaga gaatatgtaa acagccagtt gggttattac
     3601 cttttatttc tccatttgac agccatccaa attttcaagg ctcctttacc ctatagacta
     3661 atgtattatg gtagtacatc agtgatcgac agtcaatatc cgttgtattt cactgatcaa
     3721 atgatttcta agcatcaagc aaagctgatt aaagccatac attattttaa tgctgatata
     3781 ttgcaattta aacagattct ggagaattac cgtccaacag gatcctaacc caagcttaag
     3841 tcgactttgt tcccactgta cttttagctc gtacaaaata caatatactt ttcatttctc
     3901 cgtaaacaac atgttttccc atgtaatatc cttttctatt tttcgttccg ttaccaactt
     3961 tacacatact ttatatagct attcacttct atacactaaa aaactaagac aattttaatt
     4021 ttgctgcctg ccatatttca atttgttata aattcctata atttatccta ttagtagcta
     4081 aaaaaagatg aatgtgaatc gaatcctaag agaattcccc tcgaagatcg atctggcggc
     4141 cgcggatctt tgcaaagatc caaaaaaagc atgcgtacgg attagaagcc gccgagcggg
     4201 tgacagccct ccgaaggaag actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc
     4261 tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag
     4321 cttttatggt tatgaagagg aaaattggca gtaacctggc cccacaaacc tcaaatgaac
     4381 gaatcaaatt aacaaccaga tatctcgact gaaaaaaaag gtttaaacca gttccctgaa
     4441 attattcccc tacttgacta ataagtatat aaagacggta ggtattgatt gtaattctgt
     4501 aaatctattt cttaaacttc ttgaattcta cttttatagt tagtcttttt tttagtttta
     4561 aaacaccaag aacttagttt cgaataaaca cacataaata aaccatgggc atgaagtgcc
     4621 aaacatgtca cctgccgctg caactggacc catctttaga aggtcttagt ttaacccaaa
     4681 gaaatttgct actttcaaat aattcaatta tcactgcaac gaatgaaaat gtcatcagca
     4741 acaagggcat cgaagccgca gataattgtg ggcctcaaat accgaaggag aggctgaggc
     4801 gattagggga gattcagaac ataaaagact tgaatttaat agatgacaaa ttgatcactg
     4861 attcgtttgt ttttcttaat cacgatgatg atgataatgc taatattacg agtaatagca
     4921 gagaggacca gcgttatgga aatgccaacg gaaatgataa taaaaaagct aatagtgata
     4981 cttccgatgg aaccagtacc tttcgagatc atgacgaaga ggaacaagaa gctacggatg
     5041 aagacgaaaa ccaacaaata caattgaatt ccaagacttt gtccacacaa gtcaacgcaa
     5101 tgactaatgt atttaacatt ctatcttctc aaacaaacat agactttcct atctgtcagg
     5161 actgctgtaa catcttgata aacagactaa agagtgagta tgatgatgca ataaaagaga
     5221 gagatactta cgctcaattt ttatcgaagt tagagagtca aaacaaagaa atatcagaaa
     5281 gcaataaaga aaagcagtat tcccataatc tttcggaaaa agaaaatttg aaaaaggaag
     5341 aggaaagact cttggatcaa ttattacgtt tggaaatgac agatgatgac ttagatggag
     5401 aactagttcg tttacaagaa aagaaagttc aactggaaaa tgagaaactc caaaaactga
     5461 gtgatcagaa tttaatggat ttaaataaca ttcaattcaa caaaaattta cagtcattaa
     5521 aactgcaata cgaattatcc ttgaatcaat tagacaaatt gaggaaaatc aatattttca
     5581 atgctacatt caaaatctca catagcggcc cctttgccac tataaatgga ttgagattag
     5641 gcagtatacc agaatcagtg gttccttgga aggaaatcaa tgcagcactg ggccaattga
     5701 ttcttttact ggctaccata aacaaaaacc tgaagataaa tttagtggac tacgaattac
     5761 aaccgatggg ttcattttcc aaaatcaaga aaagaatggt taacagtgtc gaatataata
     5821 attccactac gaacgctccc ggtgattggt tgattctgcc tgtctactat gatgagaatt
     5881 tcaatttggg gagaatcgat gacgatgaaa cgaagtttga taaatcatta gaaacaacat
     5941 tagaaataat aagcgaaatc actcggcagc tttctacgat agcgtcatca tattcatctc
     6001 aaacactcgc aacaagccaa gatgaatctt caatgaataa cgcaaatgac gttgaaaata
     6061 gtacttccat tctcgagcta ccctatatca tgaataagga caagattaat ggattatccg
     6121 tcaaattgca tggtagcagt cccaaccttg aatggacaac ggctatgaaa tttttattaa
     6181 ccaatgtaaa gtggttgtta gcgttttctt ccaatttact atccaagtca attaccttaa
     6241 gccctacagt gaatcacaat gataagacca tcagcggaaa cggatcctaa cccaagctta
     6301 agtcgacttt gttcccactg tacttttagc tcgtacaaaa tacaatatac ttttcatttc
     6361 tccgtaaaca acatgttttc ccatgtaata tccttttcta tttttcgttc cgttaccaac
     6421 tttacacata ctttatatag ctattcactt ctatacacta aaaaactaag acaattttaa
     6481 ttttgctgcc tgccatattt caatttgtta taaattccta taatttatcc tattagtagc
     6541 taaaaaaaga tgaatgtgaa tcgaatccta agagaattcc cctcgaagat cgatctggcg
     6601 gcccgagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     6661 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6721 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6781 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6841 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6901 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6961 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7021 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7081 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7141 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7201 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7261 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7321 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7381 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7441 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7501 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7561 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7621 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7681 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7741 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7801 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7861 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7921 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7981 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8041 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8101 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8161 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8221 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8281 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8341 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8401 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8461 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8521 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8581 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8641 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8701 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8761 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaacgaa
     8821 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
     8881 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
     8941 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc
     9001 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt
     9061 accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt
     9121 tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct
     9181 cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta
     9241 ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag
     9301 ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat
     9361 tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt
     9421 atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg
     9481 tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa
     9541 tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa
     9601 aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt
     9661 tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc
     9721 gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa
     9781 gttcctatac tttctagaga ataggaactt cggaatagga acttcaaagc gtttccgaaa
     9841 acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac agctcactgt tcacgtcgca
     9901 cctatatctg cgtgttgcct gtatatatat atacatgaga agaacggcat agtgcgtgtt
     9961 tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta gtctagtacc
    10021 tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc actacccttt
    10081 agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa tgctatcatt
    10141 tcctttgata ttggatcata ctaagaaacc attattatca tgacattaac ctataaaaat
    10201 aggcgtatca cgaggccctt tcgtc
//
