LOCUS       sequence-47255-e        1957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Palmer AE, Giacomello M, Kortemme T, Hires SA, Lev-Ram V, Baker D, 
            Tsien RY
  TITLE     Ca2+ indicators based on computationally redesigned 
            calmodulin-peptide pairs.
  JOURNAL   Chem Biol. 2006 May;13(5):521-30.
  PUBMED    16720273
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2006-05"; volume: "13(5)"; pages: "521-30"
COMMENT     Sequence Label: pBAD-D2
FEATURES             Location/Qualifiers
     source          1..1957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(8..10,11..13,14..526)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             join(1232..1234,1235..1237,1238..1948)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 ggatcccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
       61 gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg agggcgatgc
      121 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      181 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca
      241 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgtac
      301 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      361 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      421 ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca
      481 gaagaacggc atcaaggccc acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      541 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      601 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      661 catggtcctg ctggagttcg tgaccgccgc ccgcatgcat gaccaactga cagaagagca
      721 gattgcagag ttcaaagaag ccttctcatt attcgacaag gatggggacg gcaccatcac
      781 cacaaaggaa cttggcaccg ttatgaggtc gcttggacaa aacccaacgg aagcagaatt
      841 gcaggatatg atcaatgaag tcgatgctga tggcaatgga acgatttact ttcctgaact
      901 tcttactatg ctggctagaa aaatgaagga cacagacagc gaagaggaaa tccgagaagc
      961 attccgtgtt tttgacaagg atgggaacgg ctacatcagc gctgctgaat tacgtcacgt
     1021 catgacaaac ctcggggaga agttaacaga tgaagaagtt gatgaaatga taagggaagc
     1081 agatatcgat ggtgatggcc aagtaaacta tgaagagttt gtacaaatga tgacagcaaa
     1141 gggggggaag aggcgctggc agaaaacagg ccatgctgtc cgaataatag gaagactgaa
     1201 gaagatctcc agctccgggg cactggagct catggtgagc aagggcgagg agctgttcac
     1261 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1321 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1381 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgat
     1441 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1501 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1561 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1621 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1681 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     1741 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1801 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     1861 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1921 cactctcggc atggacgagc tatacaagta agaattc
//
