LOCUS       sequence-47258-e        4542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Yamamoto Y, Takeshita H, Sawa H
  TITLE     Multiple Wnts redundantly control polarity orientation in 
            Caenorhabditis elegans epithelial stem cells.
  JOURNAL   PLoS Genet. 2011 Oct;7(10):e1002308. Epub 2011 Oct 13.
  PUBMED    22022276
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2011-10"; volume: "7(10)"; pages: "e1002308. Epub 
            2011 Oct 13"
COMMENT     Sequence Label: pPD95.75-wVenus
FEATURES             Location/Qualifiers
     source          1..4542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1982..2004)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2104..2123
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2201..2219)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2287..2391
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2392..2460,2461..3252)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2610..2629)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3423..4011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3912..3931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4165..4182
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4299..4320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4335..4340,4341..4358,4359..4365)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4373..4389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4378..4400
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4397..4413
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4397..4413
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
       61 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttggagggt
      121 accggtagaa aaaatgtcca aaggtgagga gcttttcaca ggagttgtgc caattctcgt
      181 cgaactcgat ggtgacgtta atggacataa gttctccgta tcgggagagg gagaaggaga
      241 tgctacttat ggaaagttaa cccttaagtt gatctgtaca acgggaaaac ttccagtgcc
      301 atgggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt ttcagccaac
      361 cctggttaca actcttggat acggattgca atgcttcgca agatacccag atcacatgaa
      421 gcgacatgac ttcttcaaaa gtgccatgcc agagggatac gtgcaggaac gtaccatttt
      481 ttttaaagac gatggaaatt acaaaacaag taagtttaaa cagttcggta ctaactaacc
      541 atacatattt aaattttcag gagccgaagt caaatttgag ggagatactc tcgttaatcg
      601 aatcgaactt aagggtatcg acttcaaaga agatggaaac atccttggac acaagttgga
      661 atacaattac aattcgcaca atgtttacat tacagcagat aagcaaaaga acggaattaa
      721 agcagtaagt ttaaacatga ttttactaac taactaatct gatttaaatt ttcagaactt
      781 caagatccgt cacaatattg aggacggagg agttcagtta gctgatcatt atcaacagaa
      841 cactccaatc ggtgacggtc cagtactttt gccagacaat cactacctgt cctaccaatc
      901 tgcactctcg aaagatccaa acgaaaagcg tgaccacatg gtccttttgg agttcgtgac
      961 cgccgctgga attacactgg gaatggatga actctacaaa tagcattcgt agaattgtaa
     1021 gtttaaactt tctcatacta actaacgcgc tctatttaaa ttttcagaat tccaactgag
     1081 cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc
     1141 agagtaagtt taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc
     1201 gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct
     1261 ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt
     1321 ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta
     1381 attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc
     1441 ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt
     1501 ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt
     1561 atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca
     1621 tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt
     1681 aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg
     1741 cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag
     1801 gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt
     1861 gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt
     1921 ttcctactag tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     1981 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     2041 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     2101 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     2161 gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat acgcctattt
     2221 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     2281 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     2341 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     2401 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2461 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2521 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     2581 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     2641 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2701 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2761 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2821 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2881 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     2941 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     3001 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     3061 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     3121 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     3181 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3241 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3301 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3361 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3421 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3481 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3541 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     3601 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3661 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3721 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3781 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     3841 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3901 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3961 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4021 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4081 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4141 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     4201 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     4261 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     4321 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     4381 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct gtaagtttaa
     4441 acatgatctt actaactaac tattctcatt taaattttca gagcttaaaa atggctgaaa
     4501 tcactcacaa cgatggatac gctaacaact tggaaatgaa at
//
