LOCUS       sequence-472626-        8721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression of ZZ-FL ScVPS30.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Rostislavleva K, Soler N, Ohashi Y, Zhang L, Pardon E, Burke JE, 
            Masson GR, Johnson C, Steyaert J, Ktistakis NT, Williams RL
  TITLE     Structure and flexibility of the endosomal Vps34 complex reveals the
            basis of its function on membranes.
  JOURNAL   Science. 2015 Oct 9;350(6257):aac7365. doi: 10.1126/science.aac7365.
  PUBMED    26450213
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2015-10-9"; volume: "350(6257):aac7365. doi"; pages: " 
            10.1126/science.aac7365"
COMMENT     Sequence Label: pYO227
FEATURES             Location/Qualifiers
     source          1..8721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1966..1985
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1966..1984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2068..2185
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     2366..2389
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             2522..2542
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2558..2731
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             2732..2905
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             2915..2935
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2966..2986
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3008..3028
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(5119..5139)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5119..5137)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5158..5174)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5158..5174)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5171..5193)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5182..5198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5206..5212,5213..5230,5231..5236))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5251..5272
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5389..5406)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5560..6148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5640..5659)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6319..7110,7111..7179))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6942..6961
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7180..7284)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7311..8653
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(8274..8321)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     8694..8712
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     1981 tagggcgaat tgggtaccgc tatgacatga ttacgaattg gcgcgccggc cgcggatctt
     2041 tgcaaagatc caaaaaaagc atgcgtacgg attagaagcc gccgagcggg tgacagccct
     2101 ccgaaggaag actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag
     2161 atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag cttttatggt
     2221 tatgaagagg aaaattggca gtaacctggc cccacaaacc tcaaatgaac gaatcaaatt
     2281 aacaaccaga tatctcgact gaaaaaaaag gtttaaacca gttccctgaa attattcccc
     2341 tacttgacta ataagtatat aaagacggta ggtattgatt gtaattctgt aaatctattt
     2401 cttaaacttc ttgaattcta cttttatagt tagtcttttt tttagtttta aaacaccaag
     2461 aacttagttt cgaataaaca cacataaata aaccatgatg ggagcagggg cgggtgctag
     2521 cgagaacctg tacttccagg gcgcccacga tgaagccgta gacaacaaat tcaacaaaga
     2581 acaacaaaac gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa
     2641 cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc
     2701 taaaaagcta aatgatgctc aggcgccgaa agtagacaac aaattcaaca aagaacaaca
     2761 aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt
     2821 catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa
     2881 gctaaatgat gctcaggcgc cgaaagtaga cgcggaaaac ctgtatttcc agggaggcgg
     2941 atcttctaga ccaactactg cctctgaaaa tttgtacttt caaggcgagt tgaagaccac
     3001 tgcttctgaa aacttgtact tccaaggtga gttactcgag ggtgaattga agactgctgc
     3061 tttggctcaa cacgacgaag ccatgggcat gaagtgccaa acatgtcacc tgccgctgca
     3121 actggaccca tctttagaag gtcttagttt aacccaaaga aatttgctac tttcaaataa
     3181 ttcaattatc actgcaacga atgaaaatgt catcagcaac aagggcatcg aagccgcaga
     3241 taattgtggg cctcaaatac cgaaggagag gctgaggcga ttaggggaga ttcagaacat
     3301 aaaagacttg aatttaatag atgacaaatt gatcactgat tcgtttgttt ttcttaatca
     3361 cgatgatgat gataatgcta atattacgag taatagcaga gaggaccagc gttatggaaa
     3421 tgccaacgga aatgataata aaaaagctaa tagtgatact tccgatggaa ccagtacctt
     3481 tcgagatcat gacgaagagg aacaagaagc tacggatgaa gacgaaaacc aacaaataca
     3541 attgaattcc aagactttgt ccacacaagt caacgcaatg actaatgtat ttaacattct
     3601 atcttctcaa acaaacatag actttcctat ctgtcaggac tgctgtaaca tcttgataaa
     3661 cagactaaag agtgagtatg atgatgcaat aaaagagaga gatacttacg ctcaattttt
     3721 atcgaagtta gagagtcaaa acaaagaaat atcagaaagc aataaagaaa agcagtattc
     3781 ccataatctt tcggaaaaag aaaatttgaa aaaggaagag gaaagactct tggatcaatt
     3841 attacgtttg gaaatgacag atgatgactt agatggagaa ctagttcgtt tacaagaaaa
     3901 gaaagttcaa ctggaaaatg agaaactcca aaaactgagt gatcagaatt taatggattt
     3961 aaataacatt caattcaaca aaaatttaca gtcattaaaa ctgcaatacg aattatcctt
     4021 gaatcaatta gacaaattga ggaaaatcaa tattttcaat gctacattca aaatctcaca
     4081 tagcggcccc tttgccacta taaatggatt gagattaggc agtataccag aatcagtggt
     4141 tccttggaag gaaatcaatg cagcactggg ccaattgatt cttttactgg ctaccataaa
     4201 caaaaacctg aagataaatt tagtggacta cgaattacaa ccgatgggtt cattttccaa
     4261 aatcaagaaa agaatggtta acagtgtcga atataataat tccactacga acgctcccgg
     4321 tgattggttg attctgcctg tctactatga tgagaatttc aatttgggga gaatcttccg
     4381 caaggaaacg aagtttgata aatcattaga aacaacatta gaaataataa gcgaaatcac
     4441 tcggcagctt tctacgatag cgtcatcata ttcatctcaa acactcgcaa caagccaaga
     4501 tgaatcttca atgaataacg caaatgacgt tgaaaatagt acttccattc tcgagctacc
     4561 ctatatcatg aataaggaca agattaatgg attatccgtc aaattgcatg gtagcagtcc
     4621 caaccttgaa tggacaacgg ctatgaaatt tttattaacc aatgtaaagt ggttgttagc
     4681 gttttcttcc aatttactat ccaagtcaat taccttaagc cctacagtga atcacaatga
     4741 taagaccatc agcggaaacg gatcctaacc caagcttaag tcgactttgt tcccactgta
     4801 cttttagctc gtacaaaata caatatactt ttcatttctc cgtaaacaac atgttttccc
     4861 atgtaatatc cttttctatt tttcgttccg ttaccaactt tacacatact ttatatagct
     4921 attcacttct atacactaaa aaactaagac aattttaatt ttgctgcctg ccatatttca
     4981 atttgttata aattcctata atttatccta ttagtagcta aaaaaagatg aatgtgaatc
     5041 gaatcctaag agaattcccc tcgaagatcg atctggcggc ccgcggccgc caccgcggtg
     5101 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     5161 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5221 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5281 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5341 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5401 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5461 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5521 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5581 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5641 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5701 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5761 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5821 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5881 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5941 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6001 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6061 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6121 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6181 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6241 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6301 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6361 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6421 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6481 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6541 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6601 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6661 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6721 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6781 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6841 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6901 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6961 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7021 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7081 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7141 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7201 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7261 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aacgaagcat
     7321 ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
     7381 atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa
     7441 gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     7501 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc tattttacca
     7561 acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc gctatttttc
     7621 taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg cagtctcttg
     7681 ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg tgtctatttt
     7741 ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta gcgaagctgc
     7801 gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat gtggattgcg
     7861 catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga
     7921 acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt
     7981 gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact
     8041 agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt
     8101 ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag
     8161 caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc cggtgcgttt
     8221 ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc tctgaagttc
     8281 ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt ccgaaaacga
     8341 gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac gtcgcaccta
     8401 tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg
     8461 cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct
     8521 gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct
     8581 gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct
     8641 ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     8701 gtatcacgag gccctttcgt c
//
